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Citations for Thermo Fisher :
1951 - 2000 of 10000+ citations for rno mir 143 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... Quantitative RT-PCRs were performed using the SYBR Green Master Mix (Thermo Fisher Scientific, Waltham, MA, USA) in a ViiA™ 7 Real-Time PCR system (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... TaqMan MicroRNA RT kit and TaqMan Universal PCR master Mix II no UNG (all from Applied Biosystems). miRNA levels were quantified by using standard curves generated from serially diluted synthetic miRNAs ...
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bioRxiv - Microbiology 2022Quote: ... RT–qPCR was performed using a StepOnePlus real-time PCR System (Applied Biosystems, Foster City, CA, USA) in standard mode (10 min at 95 °C followed by 40 cycles of 15 s at 95 °C ...
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bioRxiv - Biochemistry 2022Quote: ... cDNA was then diluted and used in RT-PCR reaction using cyber green master mix (Applied Biosystems). RT-PCR reactions were performed in QuantStudio Flex 7 instrument and data were analysed using Max studio quantification ...
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bioRxiv - Bioengineering 2022Quote: ... Proteins were analyzed in a 96-well microtiter plate using a StepOnePlus RT-PCR system (Applied Biosystems) applying a continuous heating gradient from 25 to 99°C with a step of 1% of temperature/min ...
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bioRxiv - Microbiology 2022Quote: ... and then quantified by real-time quantitative PCR (RT–qPCR) on a Viia7 light cycler (Applied Biosystems) using iTaq Universal SYBR Green Supermix (BioRad) ...
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bioRxiv - Plant Biology 2021Quote: ... The RT-qPCR reactions were done using Power SYBR Green PCR Master Mix (Applied Biosystems, Waltham, USA), 10× diluted cDNA ...
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bioRxiv - Microbiology 2020Quote: ... The viral genomic segments were amplified using the SuperScript IV One-Step RT-PCR system (Thermo Fisher) according to the previously described M-RTPCR protocol 52 ...
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bioRxiv - Cancer Biology 2020Quote: ... Duplicate RT-qPCR assays were carried out in a StepOnePlus™ Real-Time PCR System (Applied Biosystems) with SsoFast™ EvaGreen® supermix (Bio-Rad ...
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bioRxiv - Neuroscience 2020Quote: ... Reverse transcription was performed using SuperScript III first strand synthesis system for RT-PCR (Invitrogen, 18080-051) according to the manufacturer’s protocol from fibroblasts ...
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bioRxiv - Microbiology 2021Quote: ... The RT-qPCR was performed in a QuantStudio 3 Real-Time PCR System (ThermoFisher Scientific, Waltham, MA) using a Quantabio qScriptTM XLT One-Step RT-qPCR ToughMix kit (Quantabio ...
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bioRxiv - Neuroscience 2022Quote: ... RT-qPCR experiments were performed with the 7500 Fast Real-Time PCR System (Applied Biosystems/Life Technologies). All reactions were performed using primers listed in the supplementary table 3 and the iTaq Universal SYBR Green Supermix (Bio-Rad Laboratories) ...
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bioRxiv - Neuroscience 2022Quote: ... RT-qPCR experiments were performed with the 7500 Fast Real-Time PCR System (Applied Biosystems/Life Technologies). All reactions were performed using primers listed in the supplementary table 3 and the iTaq Universal SYBR Green Supermix (Bio-Rad Laboratories) ...
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bioRxiv - Neuroscience 2022Quote: ... Quantitative RT-PCR was performed in 20 µL reaction volumes containing 1X TaqMan Gene Expression (Applied Biosystems) Assay ...
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bioRxiv - Cell Biology 2022Quote: ... RT-PCR was conducted on BioRad C1000 Touch Thermal Cycler system with Phusion High Fidelity (Thermo Scientific). For RT-qPCR ...
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bioRxiv - Microbiology 2019Quote: ... RT-PCR was performed using SuperScript IV reverse transcriptase and Phusion High-Fidelity DNA polymerase (Thermo Scientific). cDNA was obtained by retrotranscription from RVFV M-segment RNA using primer MRV1ag (5’-CAAATGACTACCAGTCAGC-3’ ...
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bioRxiv - Microbiology 2019Quote: ... RT-qPCR was carried out in a StepOnePlus Real-Time PCR System (Applied Biosystems, Foster City, CA). All reactions were performed using 5× HOT FIREpol EvaGreen qPCR Mix Plus (ROX ...
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bioRxiv - Physiology 2019Quote: ... RT-qPCR was performed on a QuantStudio 5 Real-Time PCR System (Thermo Fisher Scientific, Carlsbad, California), with Fast SYBR Green master mix (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2020Quote: ... the reaction was carried out by 7500 Fast RT-PCR system (Applied Biosystems, Foster City, CA, USA). Endogenous control was glyceraldehyde 3-phosphate dehydrogenase (GAPDH) ...
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bioRxiv - Pathology 2020Quote: ... Quantitative real-time reverse transcription PCR (RT-qPCR) was performed with SYBR Select Master Mix (Applied Biosystems) using 10 ng of cDNA and 400 nM forward and reverse primers (Supplementary table 1) ...
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bioRxiv - Cancer Biology 2020Quote: ... Real-time RT-PCR was performed with Power SYBR Green master mix (Applied Biosystems, Foster City, CA) and 50ng cDNA ...
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bioRxiv - Pathology 2021Quote: ... with minor modification to adapt it to the AgPath-ID One-Step RT-PCR Kit (Life Technologies). RT-PCR targets a portion of envelope protein gene (position 26,141-26,253 of GenBank NC_004718) ...
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bioRxiv - Microbiology 2020Quote: ... Semiquantitative RT-PCR was performed with the Quantum RNA™ kit (Thermo Fisher Scientific, Dallas, TX, USA) containing primers to amplify 18S ribosomal RNA (18S rRNA ...
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bioRxiv - Microbiology 2019Quote: ... Isolated RNA (50ug) from cell lines was used as a template for RT-PCR (SuperScript III; Invitrogen). Sequences of interest were PCR amplified from cDNA using Phusion High-Fidelity mastermix (Thermo ...
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bioRxiv - Physiology 2021Quote: ... Quantitative real-time RT-PCR was performed using SYBR Premix Ex Taq with StepOne Plus (Applied Biosystems). The relative levels of Kcc2 were normalized to Gapdh ...
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A rapid, highly sensitive and open-access SARS-CoV-2 detection assay for laboratory and home testingbioRxiv - Molecular Biology 2021Quote: ... 1-step RT-qPCR was performed using the SuperScript III Platinum One-Step qRT-PCR Kit (Thermofisher) or Luna Universal One-Step RT-qPCR Kit (NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... A one-step RT-qPCR reaction was performed in a StepOnePlus Real-Time PCR System (Applied Biosystems). Cutoff points for Cq values (Cycle of quantification ...
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bioRxiv - Neuroscience 2021Quote: ... pcDNA3.1-Ptprt was cloned from mouse brain RNA by SuperScript III one-step RT–PCR system (Invitrogen). Lentiviral shRNAs against mouse ptprt were constructed in pLKO.1 (Invitrogen ...
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bioRxiv - Physiology 2021Quote: ... Quantitative reverse transcription PCR (RT-qPCR) was performed using the Universal SYBR Select Master Mix (Applied Biosystems) with a Thermal Cycler Dice TP800 system (TaKaRa) ...
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bioRxiv - Microbiology 2020Quote: ... RT-PCR was performed using the Power Up™ SYBR™ Green Master mix kit (Applied Biosystems) and the following primers ...
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bioRxiv - Developmental Biology 2020Quote: ... Quantitative RT-PCR was performed in triplicate wells using Power SYBR® Green Master Mix (Applied Biosystems) with the 7900HT Sequence Detection System (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2020Quote: ... cDNA was created using SuperScript IV® First-Strand Synthesis System for RT-PCR (ThermoFisher, Asheville, NC). Resulting samples were quantified on a Qubit 2.0 Fluorometer (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... RT-qPCR was performed using the SuperScript III Platinum One-Step qRT-PCR Kit (Thermo Fisher Scientific) following the manufacturer’s instructions with the following changes ...
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bioRxiv - Immunology 2020Quote: ... RT-qPCR was performed in triplicate using the Power SYBR Green PCR master mix (Thermo Fisher Scientific) and the Applied Biosystems Step One Plus or QuantStudio 6 Flex RT-PCR systems ...
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bioRxiv - Immunology 2020Quote: ... Downstream single cell IgG RT-PCR reactions were conducted using Superscript IV Reverse Transcriptase (Thermo Fisher, # 18090050), random hexamers (Gene Link # 26400003) ...
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bioRxiv - Immunology 2020Quote: ... Virus detection was performed using One-Step RT-PCR SuperScript(tm) III Platinum(tm) Kit (Life Technologies). Thermal cycling was achieved at 55°C for 10 minutes for reverse transcription ...
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bioRxiv - Genetics 2019Quote: ... Synthesis of cDNA was performed with SuperScript III First-Strand Synthesis System for RT-PCR (Life technologies) using random hexamers as primers ...
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bioRxiv - Immunology 2020Quote: ... Expression levels of mRNA were measured with the StepOne Plus RT-PCR system (Applied Biosystems, Burlington, ON), and the comparative Ct method was used to quantify mRNA levels using GAPDH as the normalization control.
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bioRxiv - Immunology 2022Quote: ... and used for cDNA synthesis using the SuperScript III First-Strand Synthesis System for RT-PCR (Invitrogen) as described (14 ...
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bioRxiv - Molecular Biology 2022Quote: ... RT-qPCR was carried out with a Step-One PLUS Fast Real-time PCR cycler (Applied Biosystems) under standard fast cycling conditions using PowerUP SYBR Green PCR Master Mix (Applied Biosystems ...
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bioRxiv - Physiology 2022Quote: ... RT-qPCR reactions were performed in duplicates on the ABI HT7900 real-time PCR system (Life Technologies). The expression values were calculated including the efficiency of the reactions (49 ...
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bioRxiv - Physiology 2022Quote: ... RT-qPCR reactions were performed in duplicates on the ABI HT7900 real-time PCR system (Life Technologies). The expression values were calculated including the efficiency of the reactions (49 ...
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bioRxiv - Plant Biology 2022Quote: ... The RT-qPCR was carried out on a QuantStudio 3 Real-Time PCR System (Thermo Fisher Scientific) using three independent biological samples and three technical replicates of each sample ...
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bioRxiv - Microbiology 2022Quote: Viruses were detected by RT-PCR using a 2720 Thermal Cycler (Applied Biosystems, Foster City, CA, USA) and SuperScript III One-Step RT-PCR System with Platinum Taq DNA polymerase (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... Real-time RT-PCR analysis was performed using Fast Virus 1-Step Master Mix (Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... with the final volume made up with RT-PCR grade nuclease-free water (Thermo Fisher Scientific, Germany). The PCR reactions were conducted in the Bio-Rad CFX96 real-time PCR detection system ...
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bioRxiv - Neuroscience 2022Quote: ... Gene expression levels were measured by RT-qPCR using StepOne Plus Real-Time PCR machine (Life Technologies) with FAM-labeled TaqMan probes (Thermo Scientific ...
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bioRxiv - Microbiology 2022Quote: ... The RT-qPCR was performed in a QuantStudio 3 Real-Time PCR System (ThermoFisher Scientific, Waltham, MA) using a Quantabio qScript XLT One-Step RT-qPCR ToughMix kit (Quantabio ...
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bioRxiv - Cancer Biology 2022Quote: ... The resulting cDNA was analyzed by quantitative RT-PCR (qPCR) using SYBR green master mix (Life Technologies) on QuantStudio6 Real-Time PCR system (Life Technologies) ...
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bioRxiv - Plant Biology 2023Quote: ... Quantitative RT-PCR reactions were carried out in MicroAmp Fast Optical 96-well reaction plates (Applied Biosystems) using Sybr Green Mix 2x ...