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Citations for Thermo Fisher :
1901 - 1950 of 10000+ citations for rno mir 143 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... primer/probe mixes were used together with Taqman 2× Universal PCR Master Mix (Thermofisher Scientific, cat# 4318157), as recommended by the manufacturer ...
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bioRxiv - Molecular Biology 2024Quote: ... primers and miRNAs were detected using miRNA-specific Taqman probes and Taqman Universal PCR mastermix (Thermo Fisher). All miRNAs were normalized to hsa-miR-16 ...
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bioRxiv - Biophysics 2024Quote: Mutations were generated with partially overlapping primers and PCR with Phusion Hot Start II DNA Polymerase (ThermoFisher) according to published guidelines 70 ...
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bioRxiv - Cancer Biology 2019Quote: ... First-strand cDNA synthesis was performed on 5 µg of total RNA from either fresh tumor tissue or archival FFPE CIN3 tissue using the exon 4 primer and SuperScript™II RT (Thermo Fisher Scientific) following manufacture’s protocol ...
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Enhancing gene transfer to renal tubules and podocytes by context-dependent selection of AAV capsidsbioRxiv - Cell Biology 2023Quote: ... Point eight μg of DNase-treated RNA was reverse-transcribed with a reverse transcription (RT)-specific primer using High-Capacity cDNA Reverse Transcription Kit (4368813, Applied Biosystems, Waltham, MA) or SuperScript IV Reverse Transcriptase (18090200 ...
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bioRxiv - Cell Biology 2023Quote: ... 1µg for total RNA) primed with a 1:1 ratio of random hexameric primers and oligodT using RevertAid RT kit (ThermoFisher Scientific cat# K1691). qPCR was performed on an QuantStudio5 qPCR system (Life Technologies ...
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Efficient Suppression of Endogenous CFTR Nonsense Mutations Using Anticodon Engineered Transfer RNAsbioRxiv - Molecular Biology 2021Quote: ... one-step reverse transcriptase and quantitative PCR (RT-qPCR) was performed on the QuantStudio 3 Real-Time PCR System (Applied Biosystems, Waltham, MA, USA) using the Luna Universal One-Step RT-qPCR Kit (New England BioLabs ...
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bioRxiv - Bioengineering 2020Quote: Quantitative RT-PCR analysis was performed using 20 ng cDNA per reaction using the Taqman Universal PCR Master Mix (Thermo Fisher, cat. No. 4304437) and SYBR® Green PCR Master Mix (Thermo Fisher ...
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bioRxiv - Microbiology 2022Quote: ... RNA samples were quantitated by qRT-PCR targeting NSP5 using the AgPath-ID One-Step RT-PCR Buffer and Enzyme Mix (Life Technologies, Carlsbad, CA, USA). The reactions were carried out in 20 µL reactions using NSP5 forward primer (5’-CTGGCACAGACTTAGAAGGTAACTT-3’) ...
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bioRxiv - Microbiology 2021Quote: ... Extracted RNAs from swab samples were subjected to an established IAV RT-PCR (32) targeting a conserved region of the matrix gene using the AgPath-IDTM One Step RT-PCR mix (Applied Biosystems, Foster City, CA) and an ABI 7500 real-time PCR System (Applied Biosystems ...
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bioRxiv - Immunology 2022Quote: Clones with binding-profile-of interest after secondary screening were selected and their VH and VL sequences were obtained by reverse transcription PCR (RT-PCR) using Superscript III (Thermo Fisher, catalog no. 18080044) according to manufacturer’s instruction ...
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bioRxiv - Cell Biology 2019Quote: ... real-time reverse transcription-quantitative PCR (RT-qPCR) was performed in an Applied Biosystems Step One Plus real-time PCR system (Applied Biosystems, Foster City, CA) using 2 μl cDNA diluted 1:10 ...
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bioRxiv - Pathology 2021Quote: ... The mRNA levels of cytokines and chemokines were measured with reverse transcription–polymerase chain reaction (RT-PCR) with TaqMan using a StepOnePlus real time PCR system (Thermo Fisher Scientific, Waltham, MA). We analyzed the mRNA expression level of interleukin-1β (IL-1β ...
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bioRxiv - Immunology 2020Quote: ... from each sample was reverse-transcribed using the qScript™ One-Step SYBR® Green qRT-PCR kit in a ViiA-7 RT-PCR system (Thermo Fisher Scientific). The GAPDH gene was used as the endogenous control ...
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bioRxiv - Cell Biology 2023Quote: ... Quantitative reverse transcription (RT) and real-time PCR (qRT-PCR) were done using TaqMan™ RNA-to_Ct 1-Step kit (Thermo Fisher Scientific, AB 4392938) on BioRad CFX 96 Real-time systems with the following TaqManTM probes and primers purchased from Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... and quantitative RT-PCR was performed using Power SYBR Green PCR Master Mix in a QuantStudioTM 5 thermocycler (Applied Biosystems, Foster City, CA, USA). The following immune response genes were detected ...
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bioRxiv - Developmental Biology 2024Quote: ... Katna1 transcripts were reverse-transcribed and PCR-amplified using the SuperScript™ III One-Step RT-PCR System with Platinum® Taq (ThermoFisher Scientific, France) from 200 ng of katna1+/+ and katna1-/-MZ RNA extracts using the following primers:
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bioRxiv - Molecular Biology 2023Quote: ... We measured relative expression of Ahrr and Cyp1a1 in dam lungs using quantitative real-time RT-PCR (qPCR) performed on the QuantStudio 3 Real-Time PCR System (Thermo Fisher Scientific, Waltham, MA). Ahrr and Cyp1a1 expression was quantified using the ΔΔCq method28,29 after normalizing against mean β-actin and Eif2a levels in the same sample ...
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bioRxiv - Cell Biology 2023Quote: ... Target mtDNA gene (Forward primer: 5′-CACCCAAGAACAGGGTTTGT-3′, Reverse primer: 5′-TGGCCATGGGTATGTTGTTAA-3′, Invitrogen custom primers) and reference 18S ribosomal RNA gene (Forward primer ...
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bioRxiv - Evolutionary Biology 2023Quote: ... DNA concentrations for 143 available samples from the original study were quantified using a NanoDrop One spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA). A genomic region of 226 bp comprising the rs1047626 position was amplified ...
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bioRxiv - Cancer Biology 2019Quote: ... Real time PCR analysis was performed on 1µL of cDNA using TaqMan Gene Expression Master Mix according to the manufacture’s procedures using FAM labeled TaqMan® and PrimePCR gene expression human primer/probe sets (Thermo Scientific and BioRab; see Supplementary Table 3). mRNA expression was compared to untreated control using the ΔCT method ...
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bioRxiv - Cancer Biology 2019Quote: ... or modified 5-FU-miR-15a (Dharmacon Lafayette, CO) using Oligofectamine (Thermo Fisher) following the manufacturer’s protocols.
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bioRxiv - Genetics 2019Quote: ... miR-544a inhibitors or NC inhibitors at 20 nM using Lipofectamine 3000 (Invitrogen) with serum-free media for 48h per the manufacturer’s instructions ...
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bioRxiv - Physiology 2020Quote: mirVana hsa-miR-150-5p (ID MC10070) mimic or scrambled miRNA control (Ambion) in complex with DACC lipoplex preparation (Silence Therapeutics)(Fehring et al. ...
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bioRxiv - Biochemistry 2019Quote: MiR-216a-5p inhibitor and its relative NC were compounded by Life Technologies Corporation (Carlsbad ...
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bioRxiv - Cancer Biology 2020Quote: ... with 50 nM miR-10b-5p mimic / ∼105 cells (Thermo Fisher, miRVana, 4464066) or scrambled control (Merck ...
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bioRxiv - Cancer Biology 2021Quote: ... miR-335 antagomirs were obtained from the following companies: antagomir 1 from Ambion; antagomir 2 from Exiqon (miRCURY LNA microRNA Power Inhibitor ...
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bioRxiv - Cancer Biology 2020Quote: ... miR-224 inhibitor and inhibitor Negative Control(inhibitor NC) were obtained from Ambion, Inc (Austin ...
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bioRxiv - Molecular Biology 2022Quote: ... Small-RNAs were further purified by mir-Vana miRNA isolation kit (Thermo Scientific). Small-RNA cloning was carried out as previously described (Shen et al. ...
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bioRxiv - Neuroscience 2022Quote: ... we used hsa-miR-423-5p (suggested as endogenous control by ThermoFisher, Inc) for PD ...
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bioRxiv - Neuroscience 2021Quote: ... cells were treated for 48 hours with 40nM miR-146a-5p mimic (Ambion) or mirVana miRNA mimic negative control 1 (Ambion) ...
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bioRxiv - Physiology 2023Quote: ... MiR-125b-5p (MIMAT0000423) or control mimic (Life Technologies, USA, Supplementary Table 1) were delivered to the cell culture using Lipofectamine RNAiMAX (Life Technologies ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were transfected with miR-1253 mimic (miRVanaTM miRNA mimic, ThermoFisher, 100 nM) or scramble negative control (100 nM ...
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bioRxiv - Genetics 2023Quote: ... Each miRNA (mimic miR-34c, 50pmole, #MC11039, or mimic negative control, #4464058, Thermofisher) was added to 5 ul R buffer (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... TaqMan real-time quantitative RT-PCR was performed utilizing the Applied Biosystems StepOne Plus cycler (Applied Biosystems) and TaqMan Gene Expression Assay primer and probe sets (Applied Biosystems ...
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bioRxiv - Microbiology 2019Quote: ... and tested samples using the SuperScript III Platinum One-Step Quantitative RT-PCR System with ROX (Invitrogen) and primers and probes for universal detection of influenza A and B (CDC protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... RT-qPCR was performed in triplicate using the Power SYBR Green PCR master mix (Thermo Fisher Scientific) and the Applied Biosystems Step One Plus or QuantStudio 6 Flex RT-PCR systems ...
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bioRxiv - Molecular Biology 2021Quote: ... RT-PCR amplicons were assessed by Qubit® fluorometric DNA Quantification (Thermo Fisher Scientific, Waltham, MA, USA). For samples with post RT-PCR DNA quantity below 250 ng ...
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bioRxiv - Immunology 2021Quote: ... RT-PCR was performed using the AmpliTaq Gold 360 Master Mix (Thermo Fisher Scientific, Waltham, MA, USA). The reaction mixture (20 μL ...
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bioRxiv - Developmental Biology 2019Quote: Sorted cells in RT-STA Reaction mix from the CellsDirectTM One-Step qRT-PCR Kit (Life Technologies) were kept at -80°C at least overnight before reverse transcription and specific target pre-amplification (20 cycles for single cells and 18 cycles for 20 cells) ...
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bioRxiv - Microbiology 2020Quote: ... The RT-qPCR reactions were conducted using the thermocycler StepOnePlus™ Real-Time PCR Systems (Applied Biosystems). Reactions were incubated at 50°C for 5min and 95°C for 20sec followed by 40 cycles at 95°C for 5sec and 60°C for 30sec ...
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bioRxiv - Microbiology 2020Quote: ... RT-qPCR was carried out in a StepOnePlus Real-Time PCR system (Applied Biosystems, Foster City, CA). Reactions were performed using 5× HOT FIREpol EVAGreen qPCR Mix Plus (ROX ...
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bioRxiv - Biochemistry 2021Quote: ... Quantitative real-time RT-PCR was performed on the ABI Prism 7900 Sequence Detection System (Applied Biosystems). Primer sequences were ...
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bioRxiv - Neuroscience 2021Quote: ... RT-qPCR was performed using an ABI StepOne Plus Real-Time PCR system (Thermo Fisher Scientific, Inc.) and SYBR Premix Ex Taq II master mix (Takara Biotechnology Co. ...
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bioRxiv - Neuroscience 2021Quote: ... RT-qPCR experiments were performed with the 7500 Fast Real-Time PCR System (Applied Biosystems/Life Technologies). All reactions were performed using primers ordered from Integrated DNA Technologies (IDT ...
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bioRxiv - Neuroscience 2021Quote: ... RT-qPCR experiments were performed with the 7500 Fast Real-Time PCR System (Applied Biosystems/Life Technologies). All reactions were performed using primers ordered from Integrated DNA Technologies (IDT ...
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bioRxiv - Neuroscience 2021Quote: ... and RT-qPCRs were run on a QuantStudio™ 7 Flex Real-Time PCR System (Applied Biosystems). The following primer pairs were used ...
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bioRxiv - Neuroscience 2021Quote: ... quantitative real-time PCRs (RT-qPCR) were conducted using SYBR GreenER qPCR SuperMix (Invitrogen, Carlsbad, CA, USA) for all samples in triplicates ...
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bioRxiv - Plant Biology 2020Quote: ... RT-qPCR was performed using a StepOnePlus Real-Time PCR system (Applied Biosystems, Foster City, CA, USA) or a CFX Connect Real-Time System (Bio-Rad Laboratories ...
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bioRxiv - Plant Biology 2020Quote: RT-qPCR reactions were performed using an Applied Biosystems 7500 Real-Time PCR System (Thermo Fisher Scientific). Each reaction consisted of ...