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Citations for Thermo Fisher :
1851 - 1900 of 2521 citations for 7 Octenyldimethylmethoxysilane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: Astrocytes at 3 DIV or at 7-8 DIV after lentivirus infection were loaded with 1 μg/ml Fura-2-AM (#F1221, ThermoFisher) in astrocyte culture medium for 30 min at 37 °C ...
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bioRxiv - Bioengineering 2021Quote: ... Culture medium from day 0 until day 7 was RPMI1640 (HyClone, SH30027.01) plus B-27 serum-free supplement without insulin (Gibco, A1895601). From day 7 and onwards RPMI1640 with B-27 serum free supplement with insulin (Gibco ...
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bioRxiv - Pathology 2021Quote: ... 35 mins in MES buffer for aSyn detection) and transferred to nitrocellulose membranes (0.2 μm) via Iblot (7 mins, 20 mV; Fisher Scientific). Initial blots were tested for optimisations of aSyn retention as labelled by MJFR1 either by pre-blocking heat treatment (microwaved ...
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bioRxiv - Immunology 2021Quote: ... 1 μg/ml -CD28 and 1:50 diluted conditioned supernatant from COS-7 cells transfected with a pcDNA1 vector (Invitrogen) encoding for recombinant chicken IL-2 (a kind gift from prof ...
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bioRxiv - Microbiology 2021Quote: ... cDNA was generated with 7 µl of resuspended RNA using SuperScript™ III First-strand synthesis system (Thermo Fisher Scientific), and primers A1 and A2 (Table 1 ...
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bioRxiv - Immunology 2020Quote: Cell survival was determined by analyzing forward/sideward scatter properties of cells and simultaneous staining with either Propidium iodide (PI), or 7-amino-Actinomycin D (7AAD eFluor670 dye, and LIVE/DEAD fixable violet stain (ThermoFisher), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Quantification of these genes was performed using SYBR Green on a ViiA™ 7 sequence detection system (Applied Biosystems, USA) with two replications for each sample ...
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bioRxiv - Immunology 2021Quote: ... NKp46+ and stained as indicated by manufacturer’s protocol with Cell Event Caspase 3/7 Green Flow Cytometry Assay Kit (Molecular Probes). All samples were analyzed using BD Canto FACS instrument ((BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... 2019-nCoV CDC RUO Kit (Integrated DNA Technologies) and QuantStudio™ 7 Flex Real-Time PCR System (Applied Biosystems™). PCR amplicons were quantified against in vitro transcribed RNA N gene fragment standard ...
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bioRxiv - Immunology 2020Quote: PBMC were seeded at a concentration of 7-8 × 106 cells/mL in RPMI 1640 medium without phenol red and glutamine (Gibco) supplemented with 10% heat-inactivated FBS (Gibco ...
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bioRxiv - Genomics 2021Quote: ... Apoptosis staining was performed using the CellEvent Caspase-3/7 Green Flow Cytometry Assay Kit (Thermo Fisher Scientific, Waltham, MA). Stained cell suspensions were measured with the BD LSRFortessa Cell Analyzer (BD Biosciences ...
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bioRxiv - Physiology 2020Quote: ... 1 mM fluorescent glucose analog 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Life Technologies) was diluted in Live Cell Imaging Solution (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... reverse transcription and second strand synthesis performed on the slide with cDNA quantification undertaken with qRT-PCR using KAPA SYBR FAST-qPCR kit (KAPA systems) and analysed on the QuantStudio 7-Flex system (ThermoFisher). FFPE samples were cut at the standardised 5 µm thickness onto four 6.5mm x 6.5mm capture areas ...
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bioRxiv - Immunology 2022Quote: ... The amplification reaction was monitored on the ViiA 7 Real-Time PCR System running on the QuantStudio Real Time PCR Software V1.3 (Thermo Fisher). The size of the respective amplicons was confirmed by 2% agarose gel and ethidium bromide staining ...
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bioRxiv - Cancer Biology 2022Quote: ... MCF-7 human breast cancer cells were obtained from ATCC and cultured in Roswell Park Memorial Institute medium (RPMI-1640; Gibco) supplemented with penicillin/streptomycin and 10% FCS ...
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bioRxiv - Biophysics 2022Quote: ... HEK293 and COS-7 cells were maintained at 37 ºC and 5% CO2 and cultured in Dulbecco’s modified Eagle media (Life Technologies) supplemented with 10% FBS (Hyclone ...
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bioRxiv - Cell Biology 2022Quote: Human breast cancer (MCF-7) cells were obtained from ATCC and cultured every two days in Minimum Essential Medium (MEM) Alpha media (Gibco) supplemented with 1 mM sodium pyruvate ...
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bioRxiv - Cell Biology 2022Quote: Lipid overlay assay was performed using PIP strips (nitrocellulose membrane spotted with 8 phosphoinositides and 7 other biologically relevant lipids) according to the manufacturer’s instructions (Thermo Scientific). Briefly ...
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bioRxiv - Immunology 2022Quote: ... Treg cells were stimulated for 7-8 days with Dynabeads™ Human T-Activator CD3/CD28 (1:1 beads:Treg cell ratio, Gibco), and cultured in X-VIVO 15 with Gentamicin ...
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bioRxiv - Genetics 2022Quote: ... The medium was changed to 500 μl EDM media 7 days later in 24-well flat-bottom plates for further differentiation (Nunc). After 4-7 days of culturing ...
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bioRxiv - Immunology 2022Quote: ... Bone marrow-derived macrophages (BMDMs) and dendritic cells (BMDCs) were generated for 7 days in respectively IMDM or RPMI-1640 medium (Gibco), supplemented with glutamine ...
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bioRxiv - Immunology 2022Quote: ... 5-7 × 106 cells were resuspended in 3 mL of FACS buffer (4% FBS in phosphate buffered saline (PBS, Gibco)) and sorted at the University of Massachusetts Amherst Flow Cytometry Core Facility using a BD FACSAria Fusion (Becton Dickinson) ...
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bioRxiv - Plant Biology 2022Quote: ... Entry clones were then introduced into the destination vector pH-Ubi-Cas9-7 which also encodes the Cas9-enzyme (Miao et al., 2013) using LR recombination (Invitrogen). T1 CRISPR/Cas9 lines were genotyped for the absence of the T-DNA construct by using the primers priMR410/411 (Hyg-1F/Hyg-1R) ...
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bioRxiv - Microbiology 2022Quote: ... TcdB protein was diluted to 0.05 µg/µL using phosphate buffer (100 mM KPO4, 150 mM NaCl, pH 7) containing 5X SYPRO Orange (Invitrogen S6650), and a serial dilution of test compound ...
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SLC15A4 favors inflammasome function via mTORC1 signaling and autophagy restraint in dendritic cellsbioRxiv - Immunology 2022Quote: Bone marrow cells were isolated and cultured for 7-9 d in RPMI-1640 medium (Gibco, ThermoFisher Scientific, Waltham, MA) supplemented with 10% low endotoxin-FBS (Hyclone ...
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SLC15A4 favors inflammasome function via mTORC1 signaling and autophagy restraint in dendritic cellsbioRxiv - Immunology 2022Quote: Bone marrow cells were isolated and cultured for 7-9 d in RPMI-1640 medium (Gibco, ThermoFisher Scientific, Waltham, MA) supplemented with 10% low endotoxin-FBS (Hyclone ...
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bioRxiv - Microbiology 2022Quote: ... CellEvent™ Caspase-3/7 Green Detection Reagent (C10723) and PrestoBlue™ Cell Viability Reagent (A13261) were purchased from ThermoFisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... using either a QuantStudio™ 7 Flex Real-Time PCR System or a StepOnePlus Real-Time PCR System (Applied Biosystems). Individual standard curves were generated specifically for each system ...
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bioRxiv - Microbiology 2022Quote: ... Sydney Strain SS1 (cagA+, vacA s2m2) were grown on brain heart infusion (BHI) agar (Difco, USA) containing 7% heat-inactivated horse serum (Invitrogen), antibiotics and IsoVitaleX as mentioned previously (33) ...
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bioRxiv - Microbiology 2022Quote: ... The loaded plate was amplified for 40 cycles via the ViiA 7 real-time PCR system (Thermo Fisher Scientific, Waltham). For each biological replicates (n = 3) ...
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bioRxiv - Molecular Biology 2023Quote: ... Northern blot analysis of U1 snRNA was performed using 5’-radiolabeled DNA probes (Sequence is listed in Supplemental Table 7) in ultrasensitive hybridization Buffer (Thermofisher). The radioactivity signals were analyzed by PhosphorImager (Typhoon FLA 7000).
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bioRxiv - Plant Biology 2023Quote: ... These cassettes were sub-cloned into a modified binary cas9 vector pH-Ubi-cas9-7 (Fayos et al., 2020; Miao et al., 2013) in a Gateway LR reaction (Invitrogen) to generate p-OsCPR5.1-A and p-OsCPR5.1-C constructs (Fig ...
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bioRxiv - Neuroscience 2022Quote: Larval fish (4-7 dpf) were mounted in a small drop of 2% low melting point agarose (ThermoFisher Scientific 16520) in the center of the uncoated side of a mirror galvanometer (Thorlabs GVS0111) ...
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bioRxiv - Physiology 2023Quote: ... at 1,000g for 2min and then immediately loaded into the QuantStudio 6 and 7 Flex real-time PCR system (ThermoFisher Scientific). A two-step cycling protocol was implemented to collect cycle threshold (Ct ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were resolved by 7% SDS-PAGE and transferred to nitrocellulose membrane by semi-dry i-blot transfer system (IB1001, Invitrogen). Inputs and elution fractions were analyzed on separate gels ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were resolved by 7% SDS-PAGE and transferred to nitrocellulose membrane by semi-dry i-blot transfer system (IB1001, Invitrogen). Inputs and elution fractions were analyzed on separate gels ...
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bioRxiv - Cancer Biology 2022Quote: The COS-7 cells were seeded on gold-nanoparticle-coated cover glasses and cultured overnight in DMEM (11965-092, Gibco) containing 10% FBS (10082147 ...
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bioRxiv - Synthetic Biology 2022Quote: ... 5’ and 3’ RACE ready cDNA was generated from RNA extracted from ~30 pairs of ovaries or testes dissected from 5-7 days-post-emergence (dpe) Liverpool strain adults using Trizol (Invitrogen). Primers LA1076 then nested with LA1352 (5’ ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... ABI QuantStudio (model 7 Flex) was used for quantitative PCR reactions with SYBR green master mix (Applied Biosystems; Cat #4367659). The catalog of chosen primers can be found in supplementary data (Supplementary Table 1) ...
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bioRxiv - Neuroscience 2023Quote: ... Three technical replicates per reaction was performed on the QuantStudio 7 Flex Real-Time PCR system (Applied Biosystems, Life Technologies). Data was normalized to housekeeping genes (Tubulin ...
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bioRxiv - Neuroscience 2023Quote: ... Three technical replicates per reaction was performed on the QuantStudio 7 Flex Real-Time PCR system (Applied Biosystems, Life Technologies). Data was normalized to housekeeping genes (Tubulin ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were then washed and stained for 20 min at 4 °C with the following antibodies: anti-mouse CD8α APC-efluo780 (clone 53-6-7, ebioscience/ Thermofisher), anti-mouse CD8β AF700 or BUV495 (clone YTS156 ...
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bioRxiv - Cell Biology 2023Quote: ... qRT-PCR reactions were performed on a QuantStudio 7 Flex PCR machine using PowerUp Sybr Green Master Mix (Applied Biosystems). Corresponding changes in the expression levels of selected genes were calculated using the ΔΔCt method relative to housekeeping genes ...
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bioRxiv - Neuroscience 2022Quote: ... samples were diluted to 1M urea with a blend of 468 μL of 50 mM ammonium bicarbonate (32) and 7 μg of Trypsin (ThermoFisher). The samples were subsequently incubated overnight at 25°C for digestion with trypsin ...
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bioRxiv - Immunology 2023Quote: Lungs were transferred into 7 ml bijoux tubes containing 500 µl digestion mix consisting of 5 mg/ml collagenase I (Gibco) and 10 µg/ml DNase I (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... Coding sequences were amplified using total RNA from 7 days post-fertilization (dpf) zebrafish using the SuperScript IV One-step RT-PCR kit (ThermoFisher) using the primers listed in Table S1 ...
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bioRxiv - Bioengineering 2023Quote: ... Monocytes were then differentiated into macrophages by 7–9 d of culture in IMDM + 10% AB human serum (Life Technologies).
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bioRxiv - Developmental Biology 2023Quote: ... and Ccnd1 was performed using SYBR Green qPCR Reagent (Quantabio) on a ViiA 7 Real-Time PCR machine (Applied Biosystems), as described above ...
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bioRxiv - Plant Biology 2023Quote: ... 7-day-old seedlings stably expressing LIP1-GFP were vacuum-infiltrated for 30 min with 200 nM MitoTracker Orange (Invitrogen). To test for colocalization seedlings were imaged on a confocal microscope (Zeiss LSM 780 ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then dissociated using 0.5mM EDTA into a single cell suspension and incubated in 5 μM of cell-permeant 2’,7’-dichlorodihydrofluorescein diacetate (CM-H2DCFDA) dye (Invitrogen) for 30 min at 37°C in the dark ...