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Citations for Thermo Fisher :
1901 - 1950 of 2521 citations for 7 Octenyldimethylmethoxysilane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: MCF-7 and MDA-MB 231 (was a gift from Dr Annette Meeson, Newcastle University) were cultured in DMEM (GIBCO) supplemented with 10% foetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2023Quote: ... iTaq Universal SYBR Green Supermix was used according to the manufacturer’s protocol for RT-qPCR reaction in a QuantStudio 7 Flex (Applied Biosystem by Life Technologies). qRT-PCR primers can be found in Supplementary Information Table1 ...
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bioRxiv - Developmental Biology 2023Quote: ... and Ccnd1 was performed using SYBR Green qPCR Reagent (Quantabio) on a ViiA 7 Real-Time PCR machine (Applied Biosystems), as described above ...
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bioRxiv - Microbiology 2023Quote: ... Glass coverslips with CFBE41o-cells grown for 7 days were affixed into the chamber with continuous flow of Minimal Essential Media (MEM) (GIBCO) without phenol red supplemented with 2 mM L-glutamine (GIBCO) ...
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bioRxiv - Cell Biology 2023Quote: The ROS dye assay was performed using Di(Acetoxymethyl Ester) (6-Carboxy-2’,7’-Dichlorodihydrofluorescein Diacetate) ROS dye (Invitrogen, D23844), a fluorogenic dye that is converted to 6-CarboxyFluorescein ...
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bioRxiv - Plant Biology 2023Quote: ... 7-day-old seedlings stably expressing LIP1-GFP were vacuum-infiltrated for 30 min with 200 nM MitoTracker Orange (Invitrogen). To test for colocalization seedlings were imaged on a confocal microscope (Zeiss LSM 780 ...
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bioRxiv - Cell Biology 2023Quote: ... the COS-7 cells were transfected with plasmids encoding tubulin (3×mEmerald-ensconsin) and ER (mCherry-KDEL) by using Lipofectamine 3000 (Invitrogen) according to the manufacturer’s protocol17 ...
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bioRxiv - Biochemistry 2023Quote: ... Cell proliferation was assessed at different time points (3, 5, 7 and 9 days) using AlamarBlue Cell Viability Reagent (Invitrogen) per manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... quantitative polymerase chain reaction (qPCR) was performed using a QuantStudio 7 Flex qPCR system (4485701; ThermoFisher Scientific, Waltham, MA, USA) and PowerTrack SYBR Green Master Mix (A46109 ...
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bioRxiv - Biophysics 2022Quote: The proteoliposomes were incubated with buffer IN (100 mM KCl, 20 mM HEPES pH 7, containing either 250 μM Calcein (Invitrogen), 100 μM Fura-2 (ThermoFischer Scientific) ...
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bioRxiv - Cell Biology 2022Quote: Cellular glucose uptake was measured by culturing cells in glucose free DMEM for 2 hours before adding 100µM of the fluorescent glucose analogue 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl) Amino)-2-Deoxyglucose (2-NBDG; ThermoFisher, N13195). The analogue was incubated with MCF7s for 30 minutes and MSCs for 60 minutes at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... 3.6 x 106 HEK293T17 cells were transfected on a 6-well plate with 7 µl Lipofectamine 3000 (Thermo Fisher Scientific), the packaging plasmids psPax2 (3 µg ...
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bioRxiv - Biophysics 2023Quote: ... cells were incubated with a dye master mix containing CellEventTM Caspase 3/7 Green Detection Reagent (ThermoFisher Scientific, 1:1000) and Zombie NIR fixable viability stain (BioLegend ...
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bioRxiv - Genetics 2023Quote: ... Quantitative real time PCR (n = 7–8 per genotype) was performed on an ABI 7300 Sequence Detection system (Applied Biosystems) using the PowerUp SYBR Green Master Mix Kit (Applied Biosystems).
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bioRxiv - Microbiology 2023Quote: ... approximately 5×105 Huh-7 cells were transfected with 2 µg of purified BAC DNA using Lipofectamine 3000 (Fisher Scientific) as a transfection reagent ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were passaged every 5-7 days once they reached about 70-80% confluency using TrypLE cell dissociation reagent (Gibco) and gentle scraping before being replated onto new MEFs ...
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bioRxiv - Microbiology 2023Quote: ... Quantitative Real-Time PCR was performed using KAPA SYBR FAST One-Step kit (KAPA) on a QuantStudio 7 Flex (ThermoFisher) with the following cycling conditions ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were stained with Annexin V and propidium iodide using Annexin V apoptosis detection kit (Invitrogen Cat# 50-929-7) as per manufacturers’ instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were treated with either 0.1% DMSO or 7 nM bortezomib and 30 min prior to fixation cells were treated with MitoTracker Deep Red (500 nM, Invitrogen). 16 % paraformaldehyde (EMS ...
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bioRxiv - Microbiology 2023Quote: ... 7% of heat-inactivated fetal bovine serum (FBS) and 2% of G-418 disulfate 50mg/mL (all from ThermoFisher Scientific).
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bioRxiv - Neuroscience 2023Quote: ... 7 x 106 AAV-293 cells were seeded into 150 mm cell culture plates in DMEM (# 41965062, Thermo Fisher Scientific) containing 10% fetal bovine serum (#16000-044 ...
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bioRxiv - Molecular Biology 2023Quote: The staining of oxidants was performed with the commercially available 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA) (#C6827, Thermo Fisher Scientific, USA). Cryo-sections (10 µm ...
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bioRxiv - Immunology 2023Quote: ... Bone Marrow Derived Macrophages were isolated and cultured as previously described (Schappe et al., 2018) and differentiated into mature macrophages over 7 days through culture in BMDM media (Gibco RPMI 1640 ...
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bioRxiv - Immunology 2023Quote: ... Bone Marrow Derived Macrophages were isolated and cultured as previously described (Schappe et al., 2018) and differentiated into mature macrophages over 7 days through culture in BMDM media (Gibco RPMI 1640 ...
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bioRxiv - Physiology 2023Quote: ... qPCR was performed using the QuantStudio™ 7 Flex Real Time PCR System (Applied Biosystems™, New York, NY, USA) and SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Physiology 2024Quote: ... TaqMan-based real time quantitative polymerase chain reactions (qRT-PCR) were then performed using a QuantStudio 7 Pro Real-Time PCR System (ThermoFisher). The housekeeping gene Vinculin was used as an internal control for cDNA quantification and normalization of gene amplified products.
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bioRxiv - Microbiology 2023Quote: The RAW 264.7 macrophage cell line (referred to herein as RAW cells) (ATCC TIB-71) and CD300lf-expressing Huh-7 cells were maintained in DMEM-10 medium (Gibco DMEM medium #11995-065 with 4.5 g/L D-Glucose and 110 mg/L Sodium Pyruvate ...
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bioRxiv - Neuroscience 2023Quote: ... or dominant negative (DN) CaMKII were transfected into COS-7 cells using the Lipofectamine 2000 transfection reagent (Thermo Fisher Scientific). COS-7 cells were lysed in 20 mM HEPES (pH 7.5) ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 12.5 ng/mL IL-7 (Miltenyi, USA) and 12.5 ng/mL IL-15 (Miltenyi, USA) or RPMI 1640 (Gibco, USA) medium supplemented with 100 U/mL IL-2 (Sci-Store ...
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bioRxiv - Neuroscience 2023Quote: ... Spinal cords were collected from postnatal 7–14 day old mice and placed in Hanks balance buffer solution (Gibco; UK) on ice ...
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bioRxiv - Microbiology 2023Quote: ... Five to 10 μg of total RNA were separated on a 7% (v/v) polyacrylamide gel (7.3 M urea, 1× TBE) and compared to RiboRuler LowRange RNA ladder (ThermoFisher Scientific). RNA from the gel was then blotted on a nylon membrane (Roche ...
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bioRxiv - Cancer Biology 2023Quote: ... and the Applied Biosystems® ViiATM 7 Real-Time PCR System with 384-well Block (Thermo Fisher Scientific; Cat #4453536). We used the comparative Ct (ýýCt ...
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bioRxiv - Biochemistry 2023Quote: ... buffer was exchanged to 200 mM ammonium acetate (pH 4.5) with a Zeba™ 7 K MWCO desalting column (ThermoFisher). After buffer exchange ...
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bioRxiv - Cell Biology 2023Quote: ... Cyclin B14E7E cells were selected for 72 h with Nutlin-3A 5 µM and for 7 days with Geneticin (Gibco) 0.4 mg/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... following manufacturer’s protocols and gene expression quantified via Applied Biosystems Quant Studio 6/7 Real-Time PCR System (Applied Biosystems), for TaqMan (Thermo Fisher Scientific ...
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A gene desert required for regulatory control of pleiotropic Shox2 expression and embryonic survivalbioRxiv - Developmental Biology 2023Quote: ... and on a and on a ViiA 7 Real-Time PCR System using PowerTrack SYBR Green Master Mix (Applied Biosystems) for SV-EnhΔ samples ...
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bioRxiv - Immunology 2023Quote: ... and washed with HBSS and resuspended in appropriate volume of HBSS pH 7.4 supplemented with 5% FBS before staining with 7-aminoactinomycin D (Life Technologies) to exclude dead cells ...
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bioRxiv - Neuroscience 2023Quote: ... was peeled off from the underlying tissue using a wet sterile cotton swab (7) and placed in Opti-MEM medium (Invitrogen) containing Pen-Strep ...
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bioRxiv - Biochemistry 2024Quote: HEK293 cells with Fzd1/2/4/5/7/8 knocked out were provided by Michael Boutros (Voloshanenko et al., 2017) and maintained in DMEM (Gibco) supplemented with 10% fetal bovine serum (Gemini) ...
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bioRxiv - Bioengineering 2024Quote: COS-7 cells (ATCC, CRL-1651) and HeLa cells (ATCC, CCL-2) were cultured in high-glucose DMEM (Gibco, 21063029) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2023Quote: ... organoids developed within 7 - 21 days after initial seeding and were first passaged (1:2) by dissociation using TrypLE Express Enzyme (ThermoFisher) and resuspension in OcellO primary organoid medium ...
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bioRxiv - Neuroscience 2023Quote: ... The relative levels of mRNA expression were examined utilizing real-time PCR (Applied Biosystems ViiA 7 Real-Time PCR System) with SYBR® GreenER™ reagent (Invitrogen ...
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bioRxiv - Bioengineering 2023Quote: A549 cells encapsulated in hydrogels were stained at the beginning (Day 7) and the end of culture (Day 28) with 2 µM Calcein-AM (Invitrogen) and 30 µg/mL propidium iodide (PI ...
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bioRxiv - Evolutionary Biology 2023Quote: ... gonads were then dissected in 7% MgCl2·H2O on ice and stored in RNAlater (Thermo Fisher Scientific Inc., Waltham, USA), following manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: Animals were kept individually in 6-well plates with 7 mL of 0.22 µm FASW and final concentration of 1,000 U/mL penicillin and streptomycin (Fisher Scientific, 15140122). Feeding began 3 days post amputation to allow for the gut to reform ...
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bioRxiv - Molecular Biology 2024Quote: The fluorescence-microscopy based analysis of proliferation described above for the screen was used to validate the effect of the 7 selected miRNA mimics (Dharmacon, ThermoFisher) on HPASMCs (n=4) ...
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Neuroprotective Effects of VEGF-B in a Murine Model of Aggressive Neuronal Loss With Childhood OnsetbioRxiv - Neuroscience 2023Quote: ... RT-qPCR assays were carried out in accordance with the manufacturer’s instructions on the QuantStudioTM 7 Flex RT PCR System (Applied Biosystems), utilizing the indicated amplification cycling conditions ...
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bioRxiv - Immunology 2024Quote: ... or 96-well flat-bottom plates at a concentration of 7 × 105 – 1 × 106 T cells/mL in T cell media with 10 U/mL rhIL-10 (Gibco). For both workflows ...
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bioRxiv - Neuroscience 2024Quote: ... The reaction was cycled at 58°C annealing temperature along with a melt curve analysis using a QuantStudio 7 Flex (ThermoFisher). For the telomere primers ...
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bioRxiv - Neuroscience 2024Quote: ... The reaction was cycled at 56°C annealing temperature along with a melt curve analysis using a QuantStudio 7 Flex (ThermoFisher). Each sample was run in duplicate ...