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Citations for Thermo Fisher :
2001 - 2050 of 10000+ citations for 2 4 Methyl 5 thiazolyl ethyl decanoate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... cells were treated with DMNB-caged cAMP (4,5-Dimethoxy-2-Nitrobenzyl Adenosine 3’,5’-Cyclicmonophosphate, Molecular Probes, D1037) for at least 30 min prior to imaging at a final concentration of 1 mM ...
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bioRxiv - Biochemistry 2022Quote: ... 2-3 µL of 5 µM protein solution was introduced directly into Q-Exactive UHMR mass spectrometer (ThermoFisher) through gold coated capillary needles that were prepared in-house30 ...
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bioRxiv - Cell Biology 2023Quote: Embryos were incubated for 1hr at 28ºC with 400 µM 5-ethynyl-2’-deoxyuridine (EdU, Invitrogen Cat# A5040) and 5% DMSO in E3/PTU ...
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bioRxiv - Biochemistry 2023Quote: ... 5 mM disuccinimidyl suberate (DSS) or 2 mM tert-butyl disuccinimidyl phenyl phosphonate (tBu-PhoX)(Thermo Fisher Scientific) in DMSO was added to the solution and incubated for 1 hr at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... organoids were passaged every 1-2 weeks by incubating organoids for 5-10 min in TrypLE Express (Gibco) at 37℃ and mechanical disruption to make organoids fall apart ...
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bioRxiv - Genetics 2023Quote: ... Cells were then blocked with 2 mL blocking solution made of 5% Normal Goat Serum (cat#PCN5000 Thermofisher) and 0.05% Triton X-100 in PBS for 2 hours at RT or 4°C on a shaker.
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bioRxiv - Bioengineering 2024Quote: ... 5 µL of sample were mixed with 2 µL of 1 U/µL DNase I (ThermoFisher Scientific, USA) and 13 µL of 10X DNase I reaction buffer with MgCl2 (ThermoFisher Scientific) ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were allowed to attach overnight prior to transfection with plasmid DNAs (0.5-0.75 μg/well) using Lipofectamine 2000 (2-5 μL/well; Invitrogen). Lipofection was done using small volumes of Opti-MEM (200 μL ...
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bioRxiv - Systems Biology 2024Quote: ... followed by reduction and alkylation in 5 mM tris(2 - carboxyethyl)phosphine (TCEP) (Thermo Fisher, Cat No. 77720) and 50 mM 2-Chloroacetamide (CAA ...
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bioRxiv - Cell Biology 2024Quote: ... The same test was done in parallel to assess the cell viability using MTT at 5 ug/mL (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) (Invitrogen). Sixteen sites per well were acquired using a 20x Olympus objective plan fluorite NA 0.45 (1320517 ...
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bioRxiv - Cell Biology 2024Quote: ... and then nutated in Grace’s Insect Medium containing 0.25 mg/ml 5-ethynyl-2’-deoxyuridine (EdU) (Invitrogen A10044) for 30 min at room temperature to label S-phase cells ...
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bioRxiv - Cancer Biology 2024Quote: ... 2-5 mg of minced tissue was loaded into a cuvette containing Amplex Red (Invitrogen, Waltham, MA, USA), horseradish peroxidase (5U/mL) ...
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Arc mediates intercellular synaptic plasticity via IRSp53-dependent extracellular vesicle biogenesisbioRxiv - Neuroscience 2024Quote: ... with 4% sucrose/4% formaldehyde (ThermoFisher Scientific) in 1X PBS ...
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bioRxiv - Systems Biology 2021Quote: The relative mitochondrial transmembrane potential (ΔΨm) was measured using the membrane-potential-dependent fluorescent dye TMRE (Tetramethylrhodamine, Ethyl Ester, Perchlorate) (Molecular Probes, Thermo Fisher Scientific)[87] ...
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bioRxiv - Cell Biology 2024Quote: ... aliquots of aerobic or microaerobic cells were incubated with 200 nM of membrane potential sensitive dye tetramethylrhodamine ethyl ester (TMRE, Thermo Fisher Scientific T669). Cells were first incubated in the dark with dye for 20 minutes at room temperature followed by three washes in water prior to imaging with a 561 nm laser line ...
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bioRxiv - Genetics 2023Quote: ... 4 mM 4-acetamido-4’- maleimidylstilbene-2,2’-disulfonate (AMS) (Invitrogen, catalogue no. A485), and then keep in the dark at room temperature for 1.5 hours ...
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bioRxiv - Genomics 2022Quote: ... Bisulfite-PCR was conducted using primer sets (Integrated DNA Technologies; Table 1) designed against specific genomic regions with Methyl Primer Express v1.0 software (Thermofisher Scientific). PCR amplicons were cleaned with AmpureXP beads (#A63882 ...
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bioRxiv - Biochemistry 2020Quote: ... N,N-dimethyl formamide (DMF) and water and HPLC grade methyl tert-butyl ether (MTBE) were obtained from Thermo Fisher used throughout ...
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bioRxiv - Systems Biology 2021Quote: ... A total of 90 μL of N-methyl-N-(trimethylsilyl) trifluoroacetamide (MSTFA) + 1% trimethylchlorosilane (TMCS) (Thermo Scientific, Lafayette, CO, U.S.A.) was then added to the samples and shaken at 1400 rpm for 30 min at 37 °C ...
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bioRxiv - Microbiology 2021Quote: ... The freeze-dried material was dissolved in 300 μl of 6:1 (v/v) of methyl sulphoxide D6 (99.9% atom D + 1% tetramethylsilane, ACROS Organics):D2O (100% atom D ...
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bioRxiv - Biochemistry 2021Quote: The KDELR/Legobody complex at 0.8mg/ml was PEGylated by incubation with MS(PEG)12 methyl-PEG-NHS-ester (Thermo Fisher) at a 1:40 molar ratio for 2 hrs on ice to reduce preferred particle orientation on the grids ...
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bioRxiv - Biochemistry 2022Quote: Changes in the mitochondrial membrane potential were determined as the changes in tetramethylrhodamine methyl ester (TMRM, T668, Thermo Fisher Scientific) fluorescence according to the manufacturer’s instructions with few modifications ...
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bioRxiv - Microbiology 2023Quote: ... aeruginosa growth support by pyochelin and pyochelin methyl ester were performed in M9 minimal medium supplemented with 500 µM 2,2’-Bipyridine (Fisher Scientific). M9 minimal medium was prepared as follows ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were cultured for 24 hours in culture medium supplemented with 0.5% (w/v) methyl cellulose and B-27 (1/50, Life technologies).
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bioRxiv - Molecular Biology 2020Quote: ... whole cell lysate was collected by centrifugation at 13,000 × g for 15 min at 4 °C and treated with 5 U/ml RNase T1 (Thermo Fisher Scientific) and Turbo DNase (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2020Quote: ... 10,000 freshly sorted EpCAM+ DC were transfected with 10pmol of a pool of 4 ON-Targetplus siRNA (Dharmacon) (see Supplementary Table 5) for each candidate gene using Lipofectamine RNAimax (Life Technologies) according to manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2021Quote: RNA was isolated from WT and MafAS64F/+ islets (n=4 for males, n=5 for females) using the RNAqueous total RNA isolation kit (Ambion; Thermo Fisher), and then analyzed on an Agilent 2100 Bioanalyzer ...
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bioRxiv - Cell Biology 2022Quote: Cell lysates in the SDS-PAGE sample buffer were boiled for 5 min before separating the protein on 4-12% gradient gels (Bolt Bis-Tris Plus Gels, Thermo Scientific). Proteins were then transferred onto PVDF membranes (Merck ...
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bioRxiv - Molecular Biology 2021Quote: ... was denatured at 70°C for 5 min and resolved on 4-20% Tris-borate-EDTA (TBE) Urea gel (ThermoFisher scientific) in cold 0.5 × TBE buffer (Invitrogen ...
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bioRxiv - Genetics 2020Quote: ... secondary antibodies diluted to 4 μg/ml in PBS containing 5 μg/ml Hoechst 33342 dye (Molecular probes, cat no. H3570). After treatment with secondary antibodies ...
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bioRxiv - Biochemistry 2020Quote: ... Samples were analyzed by SDS-PAGE under reducing conditions (5% β-mercaptoethanol) on 4-12% Bis-Tris NuPage Gels (Thermo Fisher) and cleavage products were detected using mouse monoclonal BC-3 antibody which detects aggrecan cleavage at the Glu392↓Ala393 bond (Cat n. ...
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bioRxiv - Biochemistry 2020Quote: ... Successful enrichment was validated by running 5 ul sample elution on NuPAGE 4-12% Bis-Tris gels followed by silver stain (ThermoFisher LC6070) following the manufacturer’s protocol.
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bioRxiv - Neuroscience 2021Quote: ... Sections were blocked in PBS-T containing 5% NDS and then incubated overnight at 4°C with rabbit anti-PSD95 (1:1000, Invitrogen/ThermoFisher) and guinea pig anti-vGlut2 (1:10,000 ...
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Insights into the secondary structural ensembles of the full SARS-CoV-2 RNA genome in infected cellsbioRxiv - Biochemistry 2021Quote: ... 1.5 μg of rRNA subtracted total RNA or 10 μg of in vitro-transcribed RNA was added to 4 μl 5× first strand buffer (ThermoFisher Scientific), 1 μl 10μM reverse primer ...
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Insights into the secondary structural ensembles of the full SARS-CoV-2 RNA genome in infected cellsbioRxiv - Biochemistry 2021Quote: ... 1.5 μg of rRNA subtracted total RNA or 10 μg of in vitro-transcribed RNA was added to 4 μl 5× first strand buffer (ThermoFisher Scientific), 1 μl 10μM reverse primer ...
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bioRxiv - Plant Biology 2021Quote: ... The membrane was rewashed with TTBS for 4 x 5 min and was developed for chemiluminescence (with agent SuperSignal West Pico, Thermo Scientific) using a LAS 4000 Image Analyzer (ImageQuant).
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bioRxiv - Biophysics 2021Quote: ... plus 1% penicillin/streptomycin in a humidified incubator at 37 °C and 5% CO2 and passaged every 3-4 days using 0.05% of Trypsin-EDTA (Thermo Fisher Scientific) to lift the cells from the culture dish ...
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bioRxiv - Neuroscience 2020Quote: Murine cortical cultures in control or supplemented media were cultured for 4 passages before being dissociated into a single cell suspension and stained with 5 µM CFSE (Molecular Probes) in PBS at 37°C for 15 minutes in the dark ...
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bioRxiv - Biochemistry 2022Quote: ... and heated at 95ºC for 5 minutes and resolved using a 4-20% tris-glycine gel (ThermoFisher Scientific, Waltham MA, XP04200). Next ...
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bioRxiv - Developmental Biology 2022Quote: ... centrifuged for 3 min at 300g at 4°C and resuspended in 1 ml pre-warmed enzymatic mix of 5 mg/ml Dispase (Gibco, 17105041) and 0.5 mg/ml Liberase TH Research Grade (Roche ...
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bioRxiv - Microbiology 2022Quote: Total RNA was isolated from 5 HIOs per group with a total of 4 replicates per condition using the mirVana miRNA Isolation Kit (Thermo Fisher). The quality of RNA was confirmed ...
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bioRxiv - Developmental Biology 2021Quote: ... transferred to 1.6 mL Eppendorf tubes and spun at 2000 rcf for 5 minutes at 4°C in an angle rotor (Thermo Scientific Fisher Heraeus Biofuge Primo R Centrifuge) ...
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bioRxiv - Molecular Biology 2022Quote: ... then the supernatant was diluted 10x and pre-cleared for 30 min at 4 °C with rotation using 5 µl protein A- and G-coupled dynabeads (ThermoFisher Scientific). An input sample was taken ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell lysates were cleared by centrifugation at 16,000 × g for 5 min at 4°C and protein concentration was determined using the Pierce BCA protein assay kit (Thermo Scientific). Cell lysates were mixed with Laemmli sample buffer (Alfa Aesar ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were cleared by centrifugation at 21,000g for 20 min at 4 °C and 5 μl/ml of RNase A/T1 (Thermo Fisher Scientific) was added to the supernatants ...
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bioRxiv - Pathology 2020Quote: ... and resolved on 4-12% Bis-Tris Plus Bolt™ gels at 4-5 ug of protein per lane in MES SDS Running Buffer (Invitrogen). Target-protein signal was detected with Horseradish Peroxidase (HRP ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples were heated to 95 °C for 5 min and cooled on ice prior to loading on NuPAGE 4-12% Bis-Tris gels (Thermo Fisher). SDS-PAGE was performed of all compared samples in parallel according to the manufacturer’s specification ...
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bioRxiv - Cancer Biology 2020Quote: ... incubated in ice for 5 min and then loaded into to a pre-cast gel (NuPAGE® 4-12% Bis-Tris Protein Gels, Invitrogen). Gel electrophoresis was performed for 90 min at 125V ...
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bioRxiv - Neuroscience 2020Quote: ... EV lysates corresponding to 5–10 µg of total protein were run into 4-12% Bis-Tris plus gels (Invitrogen, ThermoFisher Scientific). Prior to loading ...
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bioRxiv - Immunology 2020Quote: ... and heated at 70°C for 5 min before separation on 4-12% Bis-Tris Plus ready gel (Thermo Fisher Scientific). To determine PGE2R glycosylation in the hemolymph samples ...