Labshake search
Citations for Thermo Fisher :
2101 - 2150 of 10000+ citations for 2 4 Methyl 5 thiazolyl ethyl decanoate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2023Quote: ... Cells were passaged at circa 70-80% confluency or every 4 or 5 days using Accutase (7002353, Thermo Fisher / Life Technologies). Briefly ...
-
bioRxiv - Developmental Biology 2023Quote: ... Cells were passaged at circa 70-80% confluency or every 4 or 5 days using Accutase (7002353, Thermo Fisher / Life Technologies). Briefly ...
-
bioRxiv - Cell Biology 2023Quote: ... incubated in secondary antibody solution for 4 hours at RT (5% normal goat serum in PBST, Alexa Fluor 633 anti-guinea pig 1:200 (Thermo Scientific)) ...
-
bioRxiv - Cancer Biology 2023Quote: ... a final extension step (68°C for 5 min) and holding the reaction at 4°C in a thermocycler (Applied Biosystems) with a preheated lid (105°C) ...
-
bioRxiv - Cell Biology 2024Quote: ... The resulting cell suspension was passed through a 70-μm filter, centrifuged (400xg, 5 minutes, 4 °C) and resuspended in Advanced DMEM/F12 (ThermoFisher #12634010) supplemented with FCS (10% vol/vol ...
-
bioRxiv - Biochemistry 2024Quote: ... the cells were washed with 5 mL PBS (4°C) and trypsinized with 1 mL 0.25% Trypsin-EDTA per dish (Gibco, 25200-056) for 5 min at 37°C and 5% CO2 ...
-
bioRxiv - Cancer Biology 2024Quote: ... Cells were subsequently permeabilized with 0.5% Triton solution for 5 min at 4°C and then incubated with PicoGreen (1:1000 dilution) (Cat No: P11496, ThermoFisher Scientific) in PBST buffer (PBS plus 0.1% Tween-20 ...
-
bioRxiv - Bioengineering 2024Quote: ... the cells were washed again at 1224 rpm for 5 minutes at 4 degrees Celsius and then counted using an automated cell counter (Countess, Thermo Fisher) to obtain the cell concentrations ...
-
bioRxiv - Physiology 2024Quote: Intracellular Ca2+ [Ca2+]i measurements were performed in cardiomyocytes loaded with Fluo-4 AM dye (5 μmol.L-1, ThermoFisher, Cat #F14201). After a 30-minute loading period ...
-
bioRxiv - Neuroscience 2024Quote: LDS-treated samples were thawed and boiled at 95°C for 5 minutes before loading into 1.0 mm-thick Bis-Tris 4-12% polyacrylamide gels (Invitrogen, cat# NP0323) and subjected to electrophoresis ...
-
bioRxiv - Molecular Biology 2024Quote: ... The fixed cells were spun down at 900 × g for 5 min at 4°C and then washed twice with 1 ml of ice-cold PBS (Gibco 70011044). Cells were lysed twice with 1 ml of ice-cold 4C permeabilization buffer (50 mM Tris-HCl pH 7.5 ...
-
bioRxiv - Molecular Biology 2024Quote: ... 2.5 μl of the cytoplasmic fraction and 5 μl of the mitochondrial fraction were loaded onto a 4-12% Bis-Tris gel (Invitrogen, NP0322BOX) and run using MES buffer at 100V for 1 hour ...
-
bioRxiv - Cell Biology 2024Quote: ... Samples were heated at 95°C for 5 min and proteins resolved on 4–20% acrylamide Tris-glycine gradient gels (Life Technologies). Proteins were then transferred to polyvinylidene difluoride membranes (EMD Millipore ...
-
bioRxiv - Microbiology 2024Quote: ... cells were lysed with 23G needles and the parasites passed through a 5 µm filter before fixation in 4% PFA and staining with Hoechst 33342 (Thermofisher Scientific). Samples were analysed on a BD LSR Fortessa flow cytometer and with FlowJo software v10 ...
-
bioRxiv - Biophysics 2024Quote: Aliquots of 3 μL of complex sample was applied on UltrAuFoil r1.2/1.3 Grids covered with Graphene Oxide as described before76 and the grids were blotted for 3.5 s at force -1 at 4°C and plunge frozen into liquid ethane using a Vitrobot Mark IV (Thermo Fisher). 26,386 Movies were recorded on a 300 keV Titan Krios microscope (Thermo Fisher ...
-
bioRxiv - Immunology 2024Quote: ... 35 μg of protein lysate was heated at 95 °C for 5 min and loaded onto Novex Tris-Glycine Mini-Protein Gel 4–12% (ThermoFisher #XP04200BOX) and run at 100V for 1.5 h ...
-
bioRxiv - Immunology 2024Quote: ... murine lung or spleen cells were stimulated for 4 hrs with Ancestral spike (5 μg/mL) and then supplemented with Protein Transport Inhibitor cocktail (Life Technologies) for a further 10-12 hrs ...
-
bioRxiv - Biochemistry 2024Quote: ... Protein isolates were mixed with protein loading buffer containing 100 mM DTT reducing agent and denatured for 5 min at 95°C before loading onto 4–12% Bis-Tris NuPAGE precast gels (Thermo Fisher) for electrophoresis ...
-
bioRxiv - Systems Biology 2024Quote: ... about 10 cm of leaf length were cut into small pieces and placed in chilled 5-mL tubes containing 4 mL of RNALater (Fisher Scientific) solution for RNA stabilization and storage ...
-
bioRxiv - Neuroscience 2024Quote: ... The RNase I treatment was carried out as described by Ingolia et al.19 for 45 min at 4 °C using 5 µl of RNase I (Ambion, AM2295) per 250 µg of crude total RNA and quenched by adding 20 µl of SUPERase-In (Ambion ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... and the samples were run on a thermocycler (60 min, 37 °C; 5 min, 95 °C; held, 4 °C; Applied Biosystems); cDNA was stored at −20 °C until use.
-
bioRxiv - Neuroscience 2024Quote: ... The protein mixture was boiled at 95 °C for 5 minutes before run on a 4-12 % Bis-Tris gel (Invitrogen, #NP0335BOX) in NuPAGE MOPS buffer (ThermoFisher ...
-
Novel RNA-binding protein YebC enhances translation of proline-rich amino acid stretches in bacteriabioRxiv - Molecular Biology 2024Quote: ... 5 µL of the lysates were resolved on a NuPAGE 4-12% Bis-Tris Protein Gel with MOPS buffer (Thermo Scientific) and transferred to a 0.45 µm nitrocellulose membrane (Cytiva ...
-
bioRxiv - Plant Biology 2024Quote: ... Protein– antibody complexes were visualized using the alkaline phosphatase substrate 5-bromo-4-chloro-3-indolyl phosphate and nitro blue tetrazolium for color development (Life Technologies).
-
bioRxiv - Genetics 2024Quote: ... Samples were heated at 95°C for 5 min (protein denaturation step) before loading onto a 4–12% NuPAGE precast gel for Western analysis (ThermoFisher Scientific). After the transfer to PVDF membranes (LC2005 ...
-
bioRxiv - Biophysics 2024Quote: ... 1 µL of the completed PCR reactions was then combined with 4 µL of water and 5 µL of print buffer (2x: 20 mg/mL UltraPure BSA (Thermo Fisher), 24 mg/mL trehalose dihydrate ...
-
bioRxiv - Cancer Biology 2024Quote: ... Radiation-induced alterations in ZFE length were measured at 5 days post-fertilization (dpf) following embryo fixation (4% PFA) and microscopic imaging (Evos XL Core Cell Imaging System, Thermo Fisher). Analysis was conducted using ImageJ software ...
-
bioRxiv - Cell Biology 2024Quote: ... At day 4 and 5 media was replaced with Endothelial induction media composed by StemPro-34 SFM Media (10639011, Life Technologies) with StemPro-34 supplement ...
-
bioRxiv - Biophysics 2024Quote: ... The cells were allowed to attach to the grids for 4-5 h before vitrification in liquid ethane using a Vitrobot Mark IV (ThermoFisher Scientific). The following settings were used ...
-
bioRxiv - Cell Biology 2024Quote: ... Cells were centrifuged at 1000 rpm for 5 min at 4°C and resuspended in FACS buffer containing Alexa Fluor 488 goat anti-mouse IgG (Invitrogen, #A11001) at a dilution of 1:500 ...
-
bioRxiv - Cell Biology 2024Quote: ... Samples of ∼ 30 µg protein prepared with (reducing) 5% β-mercaptoethanol were separated on a 4-12% Bis-Tris NuPAGE gels (Invitrogen) and transferred to polyvinylidene-fluoride membrane for 1 h at 4°C at 40 V ...
-
bioRxiv - Cancer Biology 2024Quote: ... The cell solutions were added to cytofunnels and the samples were spun for 5 mins at 700 RPM in a Cytospin 4 centrifuge (Thermo Fisher). The slides were air-dried overnight at RT in a vertical position and the next day the cell smears were circled with a hydrophobic pen (Abcam #ab26-01 ...
-
bioRxiv - Cell Biology 2024Quote: ... Lysates were mixed with 5 µg antibody and incubated at 4°C for overnight followed by incubation with Dynabeads protein G (Life Technologies) for 4 h at 4°C ...
-
bioRxiv - Microbiology 2021Quote: ... We mixed an equal volume of the extracted LPS samples with an equal volume of 2× SDS buffer and electrophoresed 10 µl of each sample on Novex 4-20% plyacrylamide gradient gels (ThermoFisher) in Tris-Glycine-SDS buffer ...
-
bioRxiv - Cell Biology 2020Quote: ... 0.2% (v/v) Triton X-100) and analyzed by SDS-PAGE on 4-12% NuPAGE Bis-Tris gradient gels (Invitrogen) followed by immunoblotting ...
-
bioRxiv - Cell Biology 2020Quote: ... and then loaded with fluorescent Ca2+ probes (3 μM of fura-2 AM or 5.69 μM of Fluo-4 AM, Life Technologies) in HBSS for 30 minutes at 37°C ...
-
bioRxiv - Cell Biology 2020Quote: ... Cells were washed twice with 1X PBS and stained with either DAPI (4’,6-diamidino-2-phenylindole, from Life Technologies), for the visualization of the nucleus ...
-
bioRxiv - Developmental Biology 2021Quote: ... at 4°C for 2 hours with gentle agitation and subsequently with 45 μL Dynabeads Protein G (Thermo Fisher Scientific) at 4°C overnight with gentle agitation ...
-
bioRxiv - Microbiology 2021Quote: ... Slides were washed for 3 × 5 mins in PBS after each antibody step and in the penultimate wash 2μg/ml 4’,6-diamidino-2-phenylindole (DAPI) (Molecular Probes) was added ...
-
bioRxiv - Neuroscience 2022Quote: ... slices were incubated in Alexa488 Streptavidin for 2-4 hours in the same incubation solution at room temperature (S32354, Invitrogen). After final PBS washes ...
-
bioRxiv - Neuroscience 2022Quote: ... The final pellet was resuspended in 0.5 mL of NRB containing 6 μM 4’,6-diamidino-2-phenylindole (DAPI, ThermoFisher; D1306). The suspension was filtered through a 20 μm filter (Sysmex ...
-
bioRxiv - Biochemistry 2022Quote: ... at a concentration of 2 x 105 cells per ml (4 ml per dish hereafter) in DMEM (Nissui Pharmaceutical) supplemented with 10% FBS (Gibco), glutamine ...
-
bioRxiv - Molecular Biology 2020Quote: ... The suspension was incubated at 25°C for an additional 2 hr with Protein G SepharoseTM 4 Fast Flow or DynabeadsTM Protein G (Invitrogen), pre-blocked with 1 mg/ml BSA and 0.25 mg/ml Salmon Sperm DNA ...
-
bioRxiv - Neuroscience 2020Quote: ... third instar larvae were dissected in zero Ca2+ HL-3 solution at room temperature and stimulated with a HL-3 solution of 90 mM K+/2 mM Ca2+/4 μM FM4-64 (Invitrogen) for 5 min to load FM4-64 dye into the boutons ...
-
Therapy-induced lipid uptake and remodeling underpin ferroptosis hypersensitivity in prostate cancerbioRxiv - Cancer Biology 2020Quote: ... DNA and F-actin were counterstained for 20 min in the dark with 1 μL/ml 4’,6-diamidino-2-phenylindole (DAPI, Invitrogen) and Alexa Fluor 647 phalloidin (Thermo Fisher) ...
-
bioRxiv - Immunology 2021Quote: ... Supernatants were subjected to high speed centrifugation at 16,000 x g for 2 hours at 4°C (Sorvall LYNX 6000, Thermo Scientific). The virus pellet was suspended in 0.9% NaCl and sonicated on ice at 20kHz frequency and 70% amplitude for 15 seconds x 3 cycles (FB505 ...
-
bioRxiv - Immunology 2021Quote: ... then the lysate equivalent to 2×105 cells was mixed at a 1:4 ratio with 4x LDS buffer containing 10% sample reducing agent (Invitrogen). Samples were heated at 95°C for 5 minutes and collected by centrifugation before loading.
-
bioRxiv - Neuroscience 2020Quote: ... The coverslips were then mounted on slides using prolong Gold mounting medium with DAPI (4’,6-diamidino-2-phenylindole) (Invitrogen). RGCs immunohistochemistry was performed as described earlier 36–39 using SOX2 ...
-
bioRxiv - Cell Biology 2020Quote: ... proteins were desalted into reaction buffer (50 mM Tris-HCl, pH 7.5, 500 mM NaCl and 4 mM Tris (2-Carboxyethyl) Phosphine (TCEP) (Invitrogen, T2556)) for removing DTT in storage buffer ...
-
bioRxiv - Bioengineering 2021Quote: ... the cells were incubated with 2 μM EthD-1 and 4 μM Calcein AM solutions of cell viability/cytotoxicity kit (Invitrogen) for 20 minutes ...