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Citations for Thermo Fisher :
1951 - 2000 of 10000+ citations for 2 4 Methyl 5 thiazolyl ethyl decanoate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... Cells were passaged with 80-90% confluence at a split ratio of 1:2-1:4 using TrypLE (Gibco) for 8 min at 37°C ...
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bioRxiv - Bioengineering 2023Quote: ... We activate the gel surface by sulfosuccinimidyl 6-(4’-azido-2’-nitrophenylamino) (sulfo-SANPAH, Thermo Scientific, Cat. No. 22589), which allows covalent conjugation of type IV collagen to the gel surface ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were incubated with secondary antibodies for 2 hours at RT in a humid chamber and DNA was counterstained with 0.1 µg/ml of DAPI (DNA intercalator, 4’,6-Diamidino-2-Phenylindole, D1306, Invitrogen). Coverslips were mounted with ProLong Diamond Antifade (Invitrogen) ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were washed with SSCT and incubated with 600 nM 4′,6-Diamidin-2-phenylindol (Dapi, D1306, Thermo Scientific) for 10 minutes before final washing in SSCT ...
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bioRxiv - Microbiology 2023Quote: ... 2 ug of EV protein content were separated on a 4-12% Bis-Tris gel (Thermo Scientific, Rockford, IL), then blotted onto a PVDF membrane ...
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bioRxiv - Microbiology 2024Quote: ... cells were stained with either 100 nM 4′,6-diamidino-2-phenylindole (DAPI) (Thermo Fisher Scientific, Waltham, MA, USA) or Hoechst 33342 (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: shRNA transfected cells were treated with 2 mM HU for 4 h along with 0.1 µg/mL colcemid (KaryoMAX, Gibco) 30 h post-transfection ...
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bioRxiv - Microbiology 2024Quote: ... cells were mounted using Prolong gold anti-fade reagent containing 4′,6-diamidino-2-phenylindole (DAPI, Invitrogen, Carlsbad, CA). Images were captured using a Leica SP8 laser scanning confocal microscope (Leica Microsystems ...
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bioRxiv - Physiology 2024Quote: ... Slides were then stained with DAPI (1:10,000; 4’, 6-diamidino-2-phenylindole; Cat. No. D3571; Thermo Fisher Scientific) for 10 minutes at room temperature and mounted with glass coverslips using 1:1 PBS and glycerol as mounting medium ...
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bioRxiv - Neuroscience 2024Quote: ... retinas were dissected out and mounted with SlowFade mounting media containing DAPI ( 4′,6-diamidino-2-phenylindole) (Life Technologies). Images were acquired with an Axio Imager M2 optical sectioning microscope (Zeiss) ...
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bioRxiv - Microbiology 2024Quote: ... Cells that were infected with CHIKV-IOL-ZsGreen were stained with 4’,6-diamidino-2-phenylindole (DAPI, Thermo Scientific) diluted 1:1000 in PBS and incubated for 1 h at room temperature ...
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bioRxiv - Immunology 2024Quote: ... fluorescent antibodies (4 µg/mouse) and dyes (2 µl of a 10 mM Sytox Green solution; Thermo Fisher Scientific) were dissolved in 100 µl sterile PBS and injected intravenously 10 minutes before the surgery ...
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bioRxiv - Physiology 2024Quote: ... Slides were then stained with DAPI (1:10,000; 4’ ,6-diamidino-2-phenylindole; cat. no. D3571; Thermo Fisher Scientific) for 10 minutes at room temperature and mounted with glass coverslips using 1:1 PBS and glycerol as mounting medium ...
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Entorhinal cortex vulnerability to human APP expression promotes hyperexcitability and tau pathologybioRxiv - Neuroscience 2024Quote: ... 2/3rd of the eluted protein samples were resolved on a 4–12% Bris-Tris gel (Invitrogen: cat# NW04125box) and subjected to Silverstein (Pierce™ Silver Stain Kit ...
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bioRxiv - Bioengineering 2024Quote: ... PEGαMA hydrogels were made by dissolving the PEGαMA in pH 8.4 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES; Life Technologies) at 12.5-15.5 weight percent (wt%) ...
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bioRxiv - Neuroscience 2024Quote: ... After 2-4 h the coverslips were flipped over an astroglia feeder layer in Neurobasal medium (GIBCO - Life Technologies) supplemented with SM1 supplement (1:50 dilution ...
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bioRxiv - Neuroscience 2024Quote: ... After 2-4 h the coverslips were flipped over an astroglia feeder layer in Neurobasal medium (GIBCO - Life Technologies) supplemented with SM1 supplement (1:50 dilution ...
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DeFrND: detergent-free reconstitution into native nanodiscs with designer membrane scaffold peptidesbioRxiv - Biophysics 2024Quote: ... N,N’-Dimethyl-N-(Iodoacetyl)-N’-(7-Nitrobenz-2-Oxa-1,3-Diazol-4-yl)Ethylenediamine (IANBD amide) and Oregon GreenTM 488 (OG) maleimide were obtained from ThermoFisher. All other chemicals were acquired from Sigma.
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bioRxiv - Cell Biology 2024Quote: ... then fixed with 4% formaldehyde in HBSS for 2 hrs and stained with Alexa Fluor 568 phalloidin (0.5 U/ml, Invitrogen) in PBS with 0.1% Triton X-100 (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... which was boiled in the SDS loading buffer with 5% β-mercaptoethanol (Cat. #60-24-2, Acros Organics). The denatured protein samples were separated by Novex™ WedgeWell™ 4-20% SDS-PAGE Tris-Glycine gel and transferred to PVDF membrane (iBlot™ 2 Transfer Stacks ...
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bioRxiv - Genomics 2020Quote: ... Here, 40 μL pre-mix (5 μL 10x NEBuffer 2.1, 2 μL 100 mM ATP (Thermo Fisher, R0441), 3 μL 100 mM DTT ...
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bioRxiv - Developmental Biology 2021Quote: ... These vectors were co-transfected into GATA3 reporter cells (G3KI#5-2) using the Lipofectamine Stem reagent (Invitrogen). Two days after transfection ...
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bioRxiv - Immunology 2021Quote: ... we measured the proliferation capacity of NK92 cells by an EdU (5- ethynyl-2’-deoxyuridine; Invitrogen, California, USA) proliferation assay ...
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bioRxiv - Biochemistry 2020Quote: ... and an Acclaim PepMap 100 trap column (100 μm × 2 cm, nanoViper, C18, 5 μm, 100Å; Thermo Scientific) were used to separate tryptic peptides by increasing concentrations of 80% acetonitrile (can ...
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bioRxiv - Microbiology 2021Quote: ... and an Acclaim PepMap 100 trap column (100 µm × 2 cm, nanoViper, C18, 5 µm, 100Å; Thermo Scientific), the tryptic peptides were separated by increasing concentrations of 80% acetonitrile (ACN ...
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bioRxiv - Microbiology 2021Quote: ... the monolayers were overlaid with 2 ml of Minimal Essential Medium (Mediatech) supplemented with 5% FBS (Life Technologies), nonessential amino acids (Gibco) ...
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bioRxiv - Bioengineering 2022Quote: ... and blocked for 2 hours with a solution containing 0.5% TritonX-100 and 5% donkey serum (Thermo Fisher). Primary antibodies used were rat CD68 (FA-11 ...
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bioRxiv - Microbiology 2020Quote: Cells grown until A600 of ∼0.1 were labelled with 10 μM EdU (5-Ethynyl-2’-deoxyuridine, Thermofisher, C10337) for 15 min after which cells were washed ...
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bioRxiv - Neuroscience 2021Quote: ... Pipettes had resistances of 5–7 MΩ when filled with this solution supplemented with Fura-2 (Molecular Probes). Recordings were made using a Multiclamp 700B amplifier (Molecular Devices ...
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bioRxiv - Immunology 2020Quote: ... the proliferation capacity of NK92 cells was measured by an EdU (5-ethynyl-2 ‘-deoxyuridine, Invitrogen, CA, USA) proliferation assay ...
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bioRxiv - Biophysics 2021Quote: ... Cells were incubated with 10 μM of the synthetic nucleoside 5-Ethynyl-2’-deoxyuridine (EdU) (Thermo Fisher Scientific) for 25 min at 37°C and 5% CO2.
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bioRxiv - Cancer Biology 2020Quote: ... Samples were then treated with ExoSAP-IT enzyme (2 μl of enzyme for 5 μl of sample, ThermoFisher) at 37 °C for 15 min followed by a 15 min inactivation at 80 °C ...
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bioRxiv - Developmental Biology 2020Quote: ... 5-ethynyl-2’-deoxyuridine (EdU)) staining were performed using the Click-iT Plus EdU Assay kit (C10339; Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: ... Probes consisting of oligodeoxyribonucleotides (see Supplementary Table 2) were 5′-end labeled using T4 polynucleotide kinase (ThermoFisher Scientific) with 25 μCi of [γ-32P]dATP ...
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bioRxiv - Cell Biology 2021Quote: ... cells were treated with DMNB-caged cAMP (4,5-Dimethoxy-2-Nitrobenzyl Adenosine 3’,5’-Cyclicmonophosphate, Molecular Probes, D1037) for at least 30 minutes prior to imaging at a final concentration of 1 mM ...
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bioRxiv - Molecular Biology 2021Quote: ... and HeLa (ATCC CCL-2) cells were cultured at 37 °C with 5% CO2 in Dulbecco’s modified Eagle’s medium (Invitrogen) supplemented with 10% fetal bovine serum ...
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bioRxiv - Biochemistry 2021Quote: ... by heating at 95 °C for 5 min with 2 µL of SDS-PAGE loading dye (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell proliferation flow cytometry assays were performed using the 5-ethynyl-2’deoxyuridine (EdU) assay kit (Invitrogen #C10634). Cells were incubated with EdU for 24 h and labeled ...
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bioRxiv - Developmental Biology 2022Quote: ... cells were treated with 10 μM of Edu (5-ethynyl-2′-deoxyuridine; Invitrogen™, Thermo Fisher Scientific, USA), and incubated for 30 min ...
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bioRxiv - Developmental Biology 2022Quote: ... cells were treated with 10 μM of Edu (5-ethynyl-2′-deoxyuridine; Invitrogen™, Thermo Fisher Scientific, USA), and incubated for 30 min ...
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bioRxiv - Cancer Biology 2022Quote: ... The reaction was stopped by adding cold blocking buffer (5 mM EDTA, 2% fetal bovine serum (FBS) (Gibco) in HBSS) ...
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bioRxiv - Neuroscience 2024Quote: ... each animal was injected intraperitoneally with 37 mg/kg BW of 5-ethynyl-2′-deoxyuridine (EdU, ThermoFisher, A10044) at 48 hours after surgery ...
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bioRxiv - Neuroscience 2024Quote: ... each animal was injected intraperitoneally with 37 mg/kg BW of 5-ethynyl-2′-deoxyuridine (EdU, ThermoFisher, A10044) at 48 hours after surgery ...
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bioRxiv - Cell Biology 2024Quote: ... The seeded cells were allowed to attach overnight prior to transfection with plasmid DNAs (0.1-0.2 μg/well) using Lipofectamine 2000 (2-5 μL/well; Invitrogen) within a small volume of Opti-MEM (200 μL ...
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bioRxiv - Immunology 2024Quote: ... or BA.5 (2 μg/mL) spike proteins were coated onto 96-well Maxisorp plates (ThermoFisher, Cat. #439454) in 50 μL of 50 mM Na2CO3 pH 9.6 overnight at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... washed with PBS and incubated 5 minutes with PBS containing 2 µg/mL Hoechst (33342, Fisher Scientific, UK) and washed in PBS and briefly in ddH2O then mounted on glass slides using ProLong GoldTM anti-fade glass mounting medium (P36980 ...
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bioRxiv - Microbiology 2022Quote: ... and an Acclaim PepMap 100 trap column (100 μm × 2 cm, nanoViper, C18, 5 μm, 100Å; Thermo Scientific), the tryptic peptides were separated by increasing concentrations of 80% acetonitrile (ACN ...
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bioRxiv - Neuroscience 2023Quote: ... 2 x 5 min with 0.05 M PB and mounted on Superfrost Plus (ThermoFisher Catalog# 12-550-15) microscope slides ...
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bioRxiv - Neuroscience 2023Quote: ... in plating media (Neurobasal, Gibco; supplemented with 5% horse serum, Sigma; 2 mM GlutaMAX, Gibco; and 1xGS21, GlobalStem). 500,000 cortical neurons were plated per well of a 6 well plate pre-coated with PDL ...
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bioRxiv - Genetics 2023Quote: ... 3-5 million cells or approximately 15 ug of DNA crosslinked with 2% PFA (Fisher Scientific F79-500) were used as input per reaction ...