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Citations for Thermo Fisher :
1851 - 1900 of 10000+ citations for 2 4 Methyl 5 thiazolyl ethyl decanoate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... 10 μL of PageRuler Protein Ladder and 7 μg of each sample of proteins mixed with 5 μL of loading buffer were loaded and separated on a NuPAGE 4 to 12% Bis-Tris Gel (Invitrogen) using MOPS SDS Running Buffer 20X (Life technologies ...
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bioRxiv - Developmental Biology 2023Quote: ... Brains were then washed using 0.5% PBT (4×15 minutes) and incubated 1:500 with secondary antibody (anti-mouse/rabbit Alexa-488/647, Invitrogen/ThermoFisher) for 4 hours at RT before transferring to 4°C for three overnights ...
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bioRxiv - Microbiology 2023Quote: ... was used for induction of gene expression and X-gal (X-Gal 5-Bromo-4-chloro-3-indolyl-b-D-galactopyranoside; Thermofisher) TSA plates were used for bacterial assessment ...
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bioRxiv - Neuroscience 2022Quote: ... samples were mixed using either standard SDS Lämmli sample buffer and heated at 80°C for 5 min prior to loading onto gels (Novex Nupage 4-12% Bis- Tris gels, 1mm, 15 well, Invitrogen) and ran with Novex Nupage MES-SDS buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... 5% CO2 in a humidified incubator and were passaged every 3-4 days using 0.05% Trypsin-EDTA (ThermoFisher Scientific, 25300). Stable U2OS-derived cell lines ...
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bioRxiv - Neuroscience 2023Quote: ... Then the samples were heated at 95°C for 5 min and loaded onto a pre-cast Polyacrylamide 4-12% gradient gel (Bolt, Invitrogen). The proteins were then transferred onto a nitrocellulose membrane and then blocked with 10% Blotto (Santa Cruz Biotechnology ...
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bioRxiv - Molecular Biology 2023Quote: Thermal shift assays were carried out using 4 μM PhPINK1 (residues 115–575) and 5× SYPRO Orange Protein Gel Stain (Invitrogen) in 25 mM Tris (pH 8.5) ...
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bioRxiv - Plant Biology 2023Quote: ... and then rotated for another 5 min before loading the strip into the well of a NuPAGE 4-12% Bis-Tris protein gel (Invitrogen).
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bioRxiv - Microbiology 2023Quote: ... The membrane was then incubated in primary antibody in 5% milk and TBS-T overnight at 4°C at the following dilutions: 1:1000 rabbit αFLAG polyclonal (Invitrogen) and 1:1000 rabbit αHsp70 polyclonal (Abcam) ...
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bioRxiv - Microbiology 2023Quote: ... 1ml of culture was pelleted and resuspended in 30μl 1X PBS buffer containing 5 µg/ml FM 4-64 (Invitrogen #T13320) and incubated for 2 min at room temperature in the dark ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were heated at 95°C for 5 minutes before loading on a 4-12% gradient Bis-Tris gel (Invitrogen). MOPS SDS running buffer (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were centrifuged at 500 g for 5 minutes at 4°C and red blood cells were hemolyzed using ACK buffer (Gibco). White blood cells were then incubated with 1:1000 Fc Block (AbLab ...
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Striated muscle-specific base editing enables correction of mutations causing dilated cardiomyopathybioRxiv - Genetics 2022Quote: ... blocked in 5% donkey serum and incubated overnight at 4°C with a rabbit anti-Rbm20 antibody (PA5-53068, Invitrogen) at 0.5 μg/ml ...
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bioRxiv - Biophysics 2023Quote: ... denatured by heating for 5 min at 95°C and subjected to electrophoresis using precast NuPAGE Novex 4–12% Bis-Tris Proteins Gels (Invitrogen) in NuPAGE MES SDS Running Buffer (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 100mM DTT and were heated at 95°C for 5 min prior to resolving (40% of the sample) on a 4-12% Bis-Tris NuPage NOVEX gradient gel (Invitrogen) in 1x Novex NuPAGE MES SDS Running Buffer (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... NIKWT and NIKKO BMDMs were treated with 20ng/mL IL-4 for 5 hours before cells were detached using PBS containing 1mM EDTA (Gibco, ThermoFisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... The first half was treated with 1x SDS loading dye for protein visualisation and incubated at 70°C for 5 minutes and loaded onto a WedgeWell Tris Glycine 4-12% gradient gel (ThermoFisher). Coomassie Brilliant Blue (CBB ...
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bioRxiv - Microbiology 2023Quote: Cells were resuspended in PBS and incubated at 4 °C for 5 mins with 8 µg ml−1 Alexa Fluor 555 NHS ester (Invitrogen). Cells were then washed in PBS.
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bioRxiv - Molecular Biology 2023Quote: ... while TSC were passaged every 4-5 days by washing with phosphate buffered saline (1xPBS) followed by brief incubation in 0.05% Trypsin-EDTA (Gibco, 25300054) at 37°C ∼2-3 mins) ...
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bioRxiv - Neuroscience 2023Quote: ... 5 ml of Fetal Bovine Serum (Fisher) and 125 μl of a 4 mg/ml human insulin stock solution (Gibco) were added ...
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bioRxiv - Microbiology 2023Quote: ... Matched high and low molecular weight fractions adjusted to supernatant concentration equivalents in 0.9% saline were measured for fluorescence before and after addition (15 min incubation) of 5 μg/mL FM 4-64 (Invitrogen), and the background reading subtracted to obtain the final measurement.
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bioRxiv - Molecular Biology 2023Quote: ... Lysates were suspended in 710 µl of RIPA buffer with 7.5 µl PIC and incubated with gentle mixing overnight at 4 °C with 20 µl of DynabeadsTM Protein G (Invitrogen) coated with antibody against the analysed protein (listed in Supplementary Table S2) ...
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bioRxiv - Molecular Biology 2023Quote: ... 5 µg was loaded for each sample into a NuPAGETM 4-12% Bis-Tris polyacrylamide gel (ThermoFisher Scientific, catalog #NP0336BOX) and run in NuPAGETM MES SDS running buffer (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were collected (5 minutes, 1,000 rpm, 4°C) and RNA was isolated using the PureLink™ RNA Mini Kit (Ambion). RNA concentration was measured using NanoDrop 8000 (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... Two skin swab samples were taken in a 5 mL centrifuge tube and mixed with 1 mL of pre-cooled methanol-water (V:V=4:1, Thermo Fisher), after brief sonication in ice water for 20 min ...
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bioRxiv - Bioengineering 2024Quote: ... 4–5 × 104 cells were plated per well in 8-well Lab-Tek II Chamber Slides (Nunc, Rochester, NY, USA) one day before experiments ...
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bioRxiv - Immunology 2024Quote: ... The cells were washed in serum-free cRPMI medium and treated with 5 nM Fluo-4 calcium indicator dye (Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... 5 or 50 ng of each sample was loaded per lane on NuPAGE 4-12% Bis-Tris SDS-PAGE (Invitrogen) in 1X NuPAGE MOPS SDS running buffer (Invitrogen ...
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bioRxiv - Molecular Biology 2024Quote: ... they were dissociated with 0.5 mM EDTA in DPBS and split every 4-5 days at a ratio 1:8-1:12 using Revitacell Supplement (Gibco). Fibroblasts and early passage iPSCs were cultured at 37°C in 5% CO2 and frozen at passage 4-5.
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bioRxiv - Neuroscience 2024Quote: ... pH = 7.6) followed by an overnight incubation at 4°C with 1:1500 anti-mouse Tau-5 antibody (Invitrogen #AHB0042) in superblock blocking buffer ...
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bioRxiv - Immunology 2024Quote: ... heat-denatured at 95°C for 5 minutes and loaded onto a 4-12% gradient premade 1.0 mM Bis-Tris gel (Life Technologies) with molecular weight standard SeeBlue Plus2 (Life Technologies) ...
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bioRxiv - Immunology 2024Quote: ... Sorted iNKT cells were cultured in cRPMI in 96-well plates that were previously coated for 12 h at 4 °C with 5 μg/mL of anti-CD3ε (clone 145-2C11, Invitrogen) in PBS ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 300 µL of dense rosette cultures were first stained for ECM with fluorescein-jacalin (Eurobio Scientific FL-1151-5) and for membranes with FM 4-64 (Invitrogen T13320 ...
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bioRxiv - Biochemistry 2024Quote: ... 6 μg of cross-linked sample was loaded per lane and separated for 5 min on a 4-12% Bis-Tris gel (Invitrogen) using MOPS buffer ...
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bioRxiv - Bioengineering 2024Quote: ... The cells were split (at 1:6) every 4 to 5 days by treatment with Accutase (Thermo/Life Technologies; A1110501). The minimize cell loss ...
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bioRxiv - Cell Biology 2024Quote: ... Lysates were boiled at 95°C for 5 min and separated by SDS-PAGE electrophoresis using 4-12% Bis-Tris 80 NuPage gels (Invitrogen) at 180V for 70 min ...
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bioRxiv - Developmental Biology 2024Quote: ... samples were heated for 5 min at 95°C and loaded on a 4-12% Tris-Glycine gel (XP04122BOX, Invitrogen). The transfer onto a PVDF membrane (IB24001 ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were mounted on ProLong Gold with 4’,6’-diamidino-2-phenylindole (DAPI) (Thermo Fisher Scientific, Waltham, MA, USA) and imaged using a Delta-Vision II microscope system with an Olympus IX-71 inverted microscope using a 100x objective A ...
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bioRxiv - Developmental Biology 2021Quote: ... 2 x 105 – 4 x 105 mESCs were cultured in 60mm Petri dishes in DFNB medium (Neurobasal medium/Gibco, DMEM F12 1:1/Gibco ...
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bioRxiv - Neuroscience 2022Quote: ... Fluorescent indicators (fluo-4 AM, fura-2 AM, fura-FF AM) were purchased from Molecular Probes (Thermo Fisher Scientific). NMDA was from Merck Millipore ...
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bioRxiv - Molecular Biology 2020Quote: ... were resolved on precast NuPAGE 4-12% Bis-Tris midi 12+2-well protein gels (cat. no. WG1401BOX, Invitrogen) at 200 V for 40 min in NuPAGE 2-(N-morpholino)ethanesulfonic acid (MES ...
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bioRxiv - Molecular Biology 2020Quote: ... Slides were mounted in ProLong™ Gold Antifade Mountant containing 4’,6’-diamidino-2-phenylindole (DAPI; Thermo Fisher Scientific). Images were acquired at 100x magnification using a Nikon Eclipse Ti fluorescence microscope fitted with a Hamamatsu C11440 digital camera ...
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bioRxiv - Microbiology 2020Quote: ... Finally samples were counter stained for nucleus with 1 μM DAPI (4′, 6-diamidino-2′-phenylindoldihydrochloride; Thermo Fisher Scientific). Zeiss inverted Axio Observer fluorescent microscope (Zeiss ...
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bioRxiv - Immunology 2021Quote: ... Nuclei were stained with 4′,6-diamidino-2-phenylindole and were embedded with ProLong Gold mounting medium (Life Technologies). ImageJ/Fiji software (National Institutes of Health ...
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bioRxiv - Neuroscience 2020Quote: Human sections were counterstained with 4’,6-diamidino-2-phenylindole (DAPI) and coverslips were mounted using Prolong Gold (Invitrogen) for imaging ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were mounted in ProLong Gold antifade reagent supplemented with 4′,6-diamidino-2-phenylindole (DAPI; Molecular Probes). Conventional immunocytochemical staining was done to quantify the activated PAK1 and actin in oAβ and IPA-3 treated cells using phospho-PAK1 (Thr423)/PAK2 (Thr402 ...
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bioRxiv - Systems Biology 2021Quote: ... Fat and lower dermis was cut away and discarded before dispase (2 U/ml, Gibco, UK, 20h, +4°C) digestion ...
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bioRxiv - Bioengineering 2021Quote: ... cells were live-dead stained with 2 µM calcein acetoxymethyl and 4 µM ethidium homodimer-1 (#L3224, Thermo Fisher) and imaged using fluorescent microscopy to compare cell density and viability between each silicone substrate ...
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bioRxiv - Biochemistry 2021Quote: ... 2 µg PAX2 and 4 µg shRNA in pLKO.1 backbone using Lipofectamine 3000 (Thermo Fisher Scientific, Waltham, MA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... transfected with respective siRNAs (200 pmol/ well) on day 2 and day 4 using oligofectamine (Invitrogen; 10 μl/ well), and processed on day 6 for IFM or biochemical analyses ...