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Citations for Thermo Fisher :
2151 - 2200 of 10000+ citations for 2 4 Methyl 5 thiazolyl ethyl decanoate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... the sections were incubated with goat anti-rabbit IgG Alexa 488 secondary antibody (1:500 for 2 h at 4°C; Invitrogen) in solution with PBS ...
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bioRxiv - Neuroscience 2021Quote: ... in PBS for two hours at room temperature followed by 0.5 μg/mL of 4′,6-diamidino-2-phenylindole (DAPI) (ThermoFisher Scientific) in PBS for five minutes at room temperature prior to mounting.
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bioRxiv - Cell Biology 2021Quote: ... the cells were washed three times with PBS and nuclei counterstained with 1 µg/ml 4’,6-diamidino-2-phenylindole (DAPI) (#D3571, Invitrogen) in PBS for 30 min at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... Grids were blotted for 2-4 s and plunge-frozen in liquid ethane cooled by liquid nitrogen with a Vitrobot MarkIV (ThermoFisher) at 4°C and 100% humidity ...
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bioRxiv - Cell Biology 2021Quote: ... Secondary antibodies were used at 1:200 dilution in PBT for 2 hours at room temperature or at 4 °C overnight (Goat anti-Chicken Alexa 488, ThermoFisher Scientific cat#A11039 ...
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bioRxiv - Neuroscience 2020Quote: ... the sections were counterstained with DAPI (4’,6-diamidino-2-phenylinodole) and mounted onto glass slides (Superfrost Plus; Fisher Scientific) with Vectashield mounting medium (H-1200 ...
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bioRxiv - Neuroscience 2021Quote: Hair cell nuclei were specifically labeled by briefly exposing free-swimming larvae to 4’,6-Diamidino-2-Phenylindole (DAPI; Invitrogen/ThermoFisher) diluted to 2.5 μg/ml in E3 ...
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bioRxiv - Microbiology 2021Quote: ... Cells were stimulated with poly(I:C) at a final concentration of 2 μg/mL using 4 μL Lipofectamine 2000 (Thermo Fisher). At three hours post-transfection ...
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FGF10 triggers de novo alveologenesis in a BPD model: impact on the resident mesenchymal niche cellsbioRxiv - Cell Biology 2022Quote: ... incubated with secondary antibodies at RT and washed three times with TBST before being mounted with Prolong Diamond Anti-fade Mountant with DAPI (4′,6-diamidino-2-phenylindole; Invitrogen).
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bioRxiv - Neuroscience 2022Quote: ... Sections were treated with RNAScope H2O2 for 4 min at RT and subsequently washed 2 x 3 min with UltraPure Distilled Water (Invitrogen) at RT ...
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bioRxiv - Molecular Biology 2022Quote: ... Lysates were centrifuged at 17,000 × g for 10 min and supernatant incubated overnight at 4 °C with 2 μg of anti-PTBP1 antibody (monoclonal-ThermoFisher) or anti-FLAG antibody (monoclonal-Sigma) ...
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bioRxiv - Immunology 2022Quote: ... F-actin staining was performed at 4°C overnight in PBS 1x containing 0,1% TritonX100 2% FCS with Phalloidin AF405plus (Life technologies). Samples were washed in PBS 1x and mounted with Prolong Antifade Gold mounting medium (Life technologies) ...
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bioRxiv - Neuroscience 2022Quote: ... Secondary antibodies were applied in incubation buffer (1:500 in PBS/BSA) with 4‘,6-diamidino-2-phenylindole (DAPI, ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... The 4-micron Dionex IonPac AG-11-HC guard column (2 × 50 mm) and 4-micron IonPac AS11-HC analytical IC column (2.0 × 250 mm) were from Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... 10 µg input protein (typically < 2% total input protein) and 40% of IP eluates were loaded onto 4-12% Bis-Tris gradient gels (Invitrogen) and analyzed by immunoblotting.
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bioRxiv - Systems Biology 2022Quote: ... Transcriptomics using the isolated mRNA from liver tissues (0, 2, 4, 6, 8 and 10 weeks; n=3 per time point) was performed by Affymetrix GeneChip®Mouse Gene 2.0 ST Arrays (902118) ...
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bioRxiv - Genetics 2022Quote: ... fixed ovaries and eggs were incubated in a 300 nM DAPI stain solution (4′,6-diamidino-2-phenylindole, ThermoFisher Scientific) on a shaking platform (150-200 rpm ...
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bioRxiv - Developmental Biology 2022Quote: ... at 4°C for 2 hours with gentle agitation and subsequently with 45 μL Dynabeads Protein G (Thermo Fisher Scientific) at 4°C overnight with gentle agitation ...
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bioRxiv - Immunology 2020Quote: ... CAR T cells were incubated for 30 min at 37°C with either 1μM fura-2 AM or fluo-4 AM (Life Technologies). T cells were then washed in HBSS and resuspended in TexMACS complete medium with 3% AB serum ...
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bioRxiv - Microbiology 2021Quote: ... 2 μL of 10 mM NBD-(linezolid-7-nitrobenz-2-oxa-1,3-diazol-4-yl)-amino-D-alanine (NADA) (Thermo Fisher) was added to each tube and incubated at 37° C ...
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bioRxiv - Cancer Biology 2021Quote: ... ionomycin (1 μg/ml) and monensin (2 μg/ml) for 3-4 hours at 37°C in complete IMDM medium (Gibco). Viability staining was performed using the fixable viability dye eFluor780 ...
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bioRxiv - Cancer Biology 2020Quote: ... organoids were incubated with 2 mM Calcein-AM and 4 mM Ethidium homodimer-1 (LIVE/DEAD assay kit, Molecular Probes) for up to 6h ...
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bioRxiv - Bioengineering 2021Quote: ... The ovaries were dissected into 2–4 pieces and transferred into maintenance media (α-MEM, P/N 32561, Gibco, USA) and placed in a CO2 incubator prior to encapsulation.
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bioRxiv - Neuroscience 2020Quote: ... tissue was placed on slides and cover-slipped with mounting media containing DAPI (Fluoromount-G; 4’,6-diamidino-2-phenylindole; Invitrogen). All immunofluorescence runs contained tissue from more than one age (1 ...
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bioRxiv - Immunology 2021Quote: ... samples were incubated for 30 minutes at 4°C with 2 μl/ml Fixable Viability Dye eFluor506 (Thermo Fisher Scientific) while in PBS ...
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bioRxiv - Immunology 2022Quote: ... Doublets and dead cells were excluded based on forward scatter (FSC) and side scatter (SSC) and 4′,6-diamidino-2-phenylindole staining (DAPI, 1 µg/ml; ThermoFisher). All depicted flow cytometry plots were pre-gated on non-debris (by FSC and SSC) ...
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bioRxiv - Neuroscience 2022Quote: ... HEK293T cells were plated in 10cm dishes at 80-90% confluency the day before transfection and transfected 16 hours later with 4 μg total DNA (2 μg of each plasmid) using Lipofectamine 3000 (Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2022Quote: ... The cells were placed onto 4 ml MSgg agar (1.5% w/v) set into each chamber of LabTek II 2-chamber microscope vessels (Thermo Scientific) set into various containers ...
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bioRxiv - Molecular Biology 2022Quote: ... coverslips were mounted onto glass slides with mounting media containing DAPI (4’, 6-diamidino-2-phenylindole; ProLong Gold, Life Technologies).
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bioRxiv - Plant Biology 2022Quote: ... A second round of reverse transcription was initiated with the addition of 4 µL of reverse transcription mix (4:2:1:1 of First Strand Buffer, 0.1 M DTT, RNaseOUT, SuperScript II; ThermoFisher Scientific) to each sample then incubated at 25□ for 10 min ...
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bioRxiv - Developmental Biology 2020Quote: ... Nuclei were counter stained with 4′,6-diamidino-2-phenylindole (DAPI) and mounted in ProLongTM Gold antifade mounting media (Invitrogen). Images were captured using Nikon C2 confocal microscope.
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bioRxiv - Cell Biology 2020Quote: ... Primary antibodies in 2% BSA/PBS were incubated overnight at 4°C and secondary antibodies (Thermo Fisher Scientific, Alexa Fluor) were incubated for 2 hours at room temperature and washed with PBS-T ...
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bioRxiv - Microbiology 2021Quote: ... Cells were washed three times with DPBS and nuclei were counterstained with 4’,6-diamidino-2-phenylindole (DAPI) nuclear stain (300 nM DAPI stain solution in PBS; Invitrogen) for 10 minutes at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Primary microglial cells were prepared from post-natal day 2-4 pups as previously described and cultured in DMEM containing 1% PSF (ThermoFisher) 10% heat inactivated FBS (ThermoFisher ...
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bioRxiv - Cell Biology 2021Quote: ... was crosslinked to the PAAm gels using N-sulfosuccinimidyl-6-(4′-azido-2′-nitrophenylamino)hexanoate (sulfo-SANPAH, from Life Technologies). 30 μL of 1mg/mL sulfo-SANPAH dissolved in milliQ H2O was added to PAAm gels which were irradiated under a 365nm UV lamp (ABM ...
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bioRxiv - Biochemistry 2021Quote: ... with a 10-fold excess of N,N’-dimethyl-N-(iodoacetyl)-N’-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) ethylenediamine (IANBD-amide, Molecular Probes). After 90 min on ice ...
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bioRxiv - Neuroscience 2021Quote: ... P0-2) animals were removed and placed in 4°C cooled Hank’s Buffered Salt Solution (HBSS; GIBCO Life Technologies, Germany). Hippocampi were carefully dissected out and placed in Neurobasal-A Medium supplemented with B27 ...
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bioRxiv - Neuroscience 2021Quote: ... P0-2) animals were removed and placed in 4°C cooled Hank’s Buffered Salt Solution (HBSS; GIBCO Life Technologies, Germany). Hippocampi were carefully dissected out and placed in Neurobasal-A Medium supplemented with B27 ...
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bioRxiv - Neuroscience 2021Quote: Adult female flies were fed for 2-4 days on wet yeast paste and ovaries hand dissected in Grace’s Insect Medium (Life Technologies). Ovaries were fixed in 4% formaldehyde (37% formaldehyde diluted in PBST (0.2% BSA ...
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bioRxiv - Neuroscience 2021Quote: ... Coverslips were washed 3 × 5 minutes in PBS and finally mounted onto glass slides using Prolong Gold mounting medium containing 4′,6-diamidino-2-phenylindole (DAPI) (Thermofisher). Images were acquired via confocal microscopy using a Zeiss LSM-880 confocal microscope (Plan-Apochromat 40x/1.3 Oil DIC UV-IR M27objective ...
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bioRxiv - Cancer Biology 2021Quote: ... coverslips were mounted on slides using ProLong Gold Antifade Reagent with DAPI (4′,6-diamidino-2-phenylindole) (Thermo Fisher Scientific) and allowed to set overnight ...
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bioRxiv - Cell Biology 2021Quote: ... Whole brains were removed and immediately placed in 4% low-melt agarose mixed 50:50 with Neurobasal media plus B27 supplement (2% agarose final solution) (Thermofisher). Once solidified ...
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bioRxiv - Cell Biology 2020Quote: ... media was changed gradually into a gradient of (1/4) Neurobasal media (NB: based medium contained 1% Lglutamine, 1% N2 and 2% B27 (all from Invitrogen)) plus reduced gradient of (3/4 ...
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bioRxiv - Microbiology 2021Quote: ... the cells were counterstained in 1 μg/mL DAPI (4’,6-diamidino-2-phenylindole; Thermo Fisher Scientific GmbH, Bremen, Germany) for 10 min at 46°C ...
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bioRxiv - Molecular Biology 2021Quote: ... N2a cells were transfected with pSpCas9(BB)-Puro empty vector or vectors expressing each of the three gRNAs targeting the eIF4E3 exons (meIF4E3 gRNA exon 1, exon 2, exon 4) using Lipofectamine 2000 (Invitrogen). pSpCas9(BB)-Puro was a generous gift from Dr ...
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bioRxiv - Microbiology 2021Quote: ... The supernatant was then incubated overnight at 4°C and under agitation with 2 mL Ni-NTA resin (50% v/v, Thermofisher) pre-equilibrated with lysis buffer.
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bioRxiv - Developmental Biology 2021Quote: ... were crossed for 2-3 days and then transferred to fly food made from 0.6 g of Carolina Formula 4-24 (Fisher Scientific) and 2 mL of ddH2O ...
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bioRxiv - Genetics 2020Quote: ... then incubated for 2h at room temperature with Alexa Fluor secondary antibodies (S2 Table) and nuclear staining with 4’,6-diamidino-2-phenylindole (DAPI) (Invitrogen). Images were captured on a BX51 fluorescence microscope (Olympus ...
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bioRxiv - Molecular Biology 2021Quote: ... 10’000 rcf for 30 min and then ultracentrifuged at 120’000 rcf at 4 °C for 2 h (TH 64.1 rotor, Thermo Fisher Scientific). The epididymosomal pellet was then washed in PBS at 4 °C and ultracentrifuged at 120’000 rcf at 4 °C for 2 h ...
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bioRxiv - Cancer Biology 2022Quote: ... The protein lysates were combined with 6× loading buffer containing 2-mercaptoethanol and loaded on NuPAGE 4%-12% Bis-Tris Midi gels (Invitrogen). After separation of protein by SDS-PAGE ...