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Citations for Thermo Fisher :
2151 - 2200 of 10000+ citations for Mouse Presenilin Enhancer 2 Homolog C. Elegans PSENEN ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2024Quote: ... Cells were loaded at 37°C for 60 minutes with 4 μM Fura-2-AM in the presence of 1X Power Load permeabilizing reagent (Thermo Fisher). Injection of AngII (10-8M) ...
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bioRxiv - Biophysics 2024Quote: ... followed by a 2-minute incubation at 37 °C (with gentle rotation) in 1 mL warm TrypLE (Thermo Fisher Scientific, #12604013). The majority of TrypLE was removed by pipetting after incubation ...
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bioRxiv - Molecular Biology 2024Quote: ... MDA-MB-231 and HEK-293T RNAs (5 μg) were incubated with 2 U TURBO DNase for 30 min at 37 °C (Thermo Fisher). After DNase digestion ...
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bioRxiv - Bioengineering 2024Quote: ... 3D iHeps (Day 22) were incubated for 2 hours with HCM media containing 1μmol/L of 6-CDF (Thermofisher, #C-369). Samples were then washed with PBS for 15 minutes before staining with Hoescht in HCM.
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bioRxiv - Cell Biology 2021Quote: ... cells were pulsed with 10µM 5-Ethynyl-2’deoxyuridine (EdU) for 2 hours following manufacturer’s instructions (Click-iT® EdU Alexa Fluor® Imaging Kit, Life Technologies). Cells were subsequently fixed and stained with Hoechst 33342 ...
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bioRxiv - Microbiology 2023Quote: The purified PCR products were cloned into pCR-XL-2-TOPO™ vectors using the TOPO™ XL-2 Complete PCR Cloning Kit (Invitrogen). After incubation ...
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bioRxiv - Cell Biology 2021Quote: CFAP61 was immunoprecipitated from mouse testis using the Pierce crosslink IP kit (Thermo Scientific) with anti-CFAP61 antibody described above ...
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bioRxiv - Cancer Biology 2022Quote: RNA was extracted from frozen mouse tissues using the Purelink RNA Extraction Kit (Invitrogen). Tissues were homogenized with a rotor-stator homogenizer in RNA lysis buffer containing 1% β-mercaptoethanol and RNA was isolated following manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... following standard protocols supplied in the Pierce Mouse IgG1 Fab Preparation Kit (Thermo Fisher). Complexes of refolded MAGEA4 pMHC bound to 2M2 Fab were isolated by mixing the two components and separating the complex by SEC using a Superdex 200 Increase column ...
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bioRxiv - Microbiology 2022Quote: ... and Dynabeads™ Untouched™ Mouse CD4 Cells Kit were purchased from Thermo Fisher Scientific Inc ...
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bioRxiv - Systems Biology 2022Quote: ... To enrich for lineage negative (Lin-) cells Dynabeads Untouched Mouse CD4 Cells kit (Invitrogen) were used according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... and depleted of rRNA by the RiboMinus™ Human/Mouse Transcriptome Isolation Kit (Invitrogen). RNA was then reverse transcribed by the SMARTer PCR cDNA Synthesis Kit (Clontech ...
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bioRxiv - Cancer Biology 2024Quote: ... Genomic DNA was isolated from total mouse splenocytes using PureLink Genomic DNA kit (Invitrogen). 500ng of DNA was subsequently used for library preparation using NEBNext® Ultra™ II FS DNA Library Prep Kit for Illumina® following manufacturer’s recommendations ...
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bioRxiv - Immunology 2022Quote: ... CD8+ T cells were enriched using Dynabeads Untouched Mouse CD8 Cells Kit (Invitrogen, #11417D) according to manufacturer’s instructions.
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bioRxiv - Cancer Biology 2023Quote: ... and brachial lymph nodes with the Dynabeads Mouse DC Enrichment Kit (cat# 11429D, Invitrogen) according to the manufacture’s protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... RNA extraction from mouse kidneys was performed using the PureLink RNA Mini kit (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Naive CD4+ T cells were isolated via Dynabeads untouched mouse CD4 isolation kit (ThermoFisher) according to kit specifications ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were isolated using Dynabeads™ FlowComp™ Mouse CD8 Kit (Invitrogen, CAT#: 11462D) and treated with enough antibody for the number of cells isolated ...
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bioRxiv - Immunology 2023Quote: ... mouse anti-mouse PCSK9 (Invitrogen), mouse anti-mouse vinculin (Santa Cruz) ...
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bioRxiv - Cell Biology 2020Quote: ... The cells were labeled with the rabbit anti-tau (C-terminal) antibody (MilliporeSigma) or the mouse anti-phospho-Tau (Thr181) Clone AT270 antibody (Thermo Fisher Scientific) at room temperature at dilution of 1:1000 in 1% BSA for 1 hour and then labeled with Alexa Fluor488 goat anti-mouse IgG secondary antibody at a dilution of 1:500 for 30 minutes at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... 1:40 and anti-GFP 1:400 Rockland 600-101- 215 were incubated 24h at 4° C followed by secondary antibodies: Alexa 568 anti-mouse 1:200 (Thermo Fisher A10037), Alexa 568 anti-Rabbit (Thermo Fisher A10042) ...
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bioRxiv - Neuroscience 2019Quote: ... The blots were blocked in PBS containing 5% skim milk and then probed with the following primary antibodies over night at 4°C: mouse anti-total tau (HT7, 1:1000, Thermo Fisher #MN1000), mouse anti-phospho tau Ser202/Thr205 (AT8 ...
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bioRxiv - Microbiology 2021Quote: ... Cells infected with YFV were incubated with the pan-flavivirus anti-Env 4G2 antibody for 1h at 4°C and then with Alexa 488 anti-mouse IgG secondary antibodies (Thermo Fisher, #A28175) for 45 min at 4°C in the dark ...
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bioRxiv - Molecular Biology 2020Quote: ... the sections were incubated at 4°C overnight with anti-puromycin antibody and visualized using an Alex Fluor 568 conjugated goat anti-mouse IgG (Thermo Fisher, A11019). Nuclei were stained with DAPI (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2021Quote: ... the samples were washed with PBS and incubated in the dark for 1 h at 4 °C with secondary antibody (Alexa Fluor-568-conjugated anti-mouse antibody, 1:750, Thermo Fisher Scientific). Finally ...
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bioRxiv - Cancer Biology 2022Quote: ... Sections were washed 3 times for 10 min in PBS 1X + 0.1% Triton X-100 + 1% BSA and incubated for 6h with secondary antibodies at 4°C: goat anti-mouse IgG (H+L) highly cross-adsorbed secondary antibody Alexa Fluor 568 (1/500, Invitrogen #A-11031), goat anti-rabbit IgG (H+L ...
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bioRxiv - Microbiology 2022Quote: ... cultures were washed three times with 1X PBS and then stained overnight at 4°C with rabbit anti-mouse AlexaFluor ™488-conjugated antibody (Thermo Fisher) diluted 1:1,000 in stain buffer ...
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bioRxiv - Physiology 2022Quote: 60 x 106 of mouse splenocytes were incubated for 15 min at 4°C in PBS containing 5% Normal Rat Serum (Thermo Scientific, 10710C) and anti-CD16/32 (BioLegend ...
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bioRxiv - Molecular Biology 2020Quote: ... The cleared lysate was incubated for 60 min at 4°C with 50 μL of pre-washed magnetic Pan Mouse Dynabeads (Thermo Fisher Scientific) coupled to 15 μL of anti-HA F7 mouse monoclonal antibody (Santa Cruz Biotechnology) ...
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bioRxiv - Neuroscience 2022Quote: ... Incubations were performed for 14h at 4° C followed by incubation with secondary antibodies: Alexa 568 anti-mouse 1:200 (Thermo Fisher A10037), and Alexa 488 anti-goat 1:200 (Thermo Fisher A11055 ...
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bioRxiv - Developmental Biology 2022Quote: ... 2014)) were initially cultured on mitomycin C inactivated mouse embryonic fibroblasts (MEFs) feeder cells using DMEM-F12 (Life Technologies cat# 11330-057) supplemented with 15% FBS (Hyclone SH30396.03) ...
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bioRxiv - Neuroscience 2022Quote: ... iPSCs were maintained on irradiated mouse CF-1 feeder layer (ATCC, Manassas, VA) at 37 °C and 5% CO2 in Knockout DMEM medium (Invitrogen, Waltham, MA) supplemented with 20% Knockout Serum Replacer (KSR ...
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Interactome comparison of human embryonic stem cell lines with the inner cell mass and trophectodermbioRxiv - Developmental Biology 2019Quote: ... hESCs (p21-27) were cultured and expanded on Mitomycin C inactivated mouse embryonic fibroblasts (iMEFs) in hESC medium KO-DMEM (Invitrogen, Paisley, UK) with 20% knockout serum replacement (KO-SR ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Brains were washed again as before and then incubated for 3h at room temperature followed by 24h at 4 °C with Alexa Fluor Plus 488 Goat anti-mouse (Life Technologies # A32723) and Alexa Fluor Plus 555 goat anti-rabbit (Life Technologies # A32732 ...
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bioRxiv - Cell Biology 2021Quote: ... at 4°C overnight and then incubated with horseradish peroxidase (HRP)-conjugated secondary anti-mouse or rabbit antibodies (Thermo Fisher, Waltham, MA). Protein expression was detected by using enhanced chemiluminescence (ECL ...
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bioRxiv - Bioengineering 2019Quote: ... 5×106 cells were labeled with a 1:100 dilution of chicken anti-c-myc antibody or mouse anti-V5 antibody (Thermo Fisher Scientific) for 15 minutes at room temperature ...
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bioRxiv - Physiology 2021Quote: ... and then incubated overnight at 4°C with AF594-conjugated goat anti-mouse IgG1 secondary antibody (A21125, Invitrogen, USA; 1:500 dilution) and AF488-conjugated α-bungarotoxin (B13422 ...
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bioRxiv - Physiology 2021Quote: ... and then incubated overnight at 4°C with AF594-conjugated goat anti-mouse IgG1 secondary antibody (A21125, ThermoFisher, USA; 1:500 dilution), Cy5 goat anti-rabbit IgG (H&L ...
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bioRxiv - Immunology 2021Quote: Homogenates collected after douncing of tissue from the footpads of infected animals were spun down to remove cellular components and supernatants were collected and stored at - 80°C for cytokine measurements using a Th1-Th2 mouse ProcartaPlex Panel (Thermo Fisher Scientific). 50ul of supernatants with appropriate dilutions were used for cytokine estimations and fluorescent readings were acquired on a BioRad Bio-Plex (Luminex 200 ...
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bioRxiv - Genomics 2021Quote: ... Cat# ABIN6952432) overnight at 4 ?C followed by staining with Alexa Fluor 647-conjugated secondary antibody (goat anti-mouse, Thermo Fisher, A21235) and DAPI (Thermo FIsher) ...
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bioRxiv - Neuroscience 2022Quote: ... Secondary antibody incubation was also performed overnight at 4°C with donkey anti-mouse Alexa 647 (1:250, Invitrogen, Cat. # A-31571).
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bioRxiv - Neuroscience 2022Quote: ... Slices were incubated overnight at 4°C in a 1:500 dilution of primary antibody: Tyrosine Hydroxylase (TH, mouse, Invitrogen; MA1-24654) and the immediate early gene protein c-Fos (c-Fos ...
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bioRxiv - Neuroscience 2022Quote: ... slices were incubated overnight at 4°C with goat anti-mouse Alexa Fluor 568 and goat anti-rabbit Alexa Fluor 488 (ThermoFisher A-11008), both diluted 1:500 in blocking solution ...
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bioRxiv - Molecular Biology 2024Quote: ... mESC were cultured over mitomycin C-treated mouse embryonic fibroblasts (MEFs) as feeder cells and using knockout DMEM medium (ThermoFisher Scientific, 10829018) supplemented with LIF ...
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bioRxiv - Microbiology 2024Quote: ... for HSV-1 in blocking buffer for 1 h at 4°C and stained with secondary antibody goat anti-mouse/rabbit AlexaFuor680 (Thermo Fisher Scientific) in blocking buffer at RT for 1h ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were washed 3 x 7 min with PBS and incubated overnight at +4°C with goat anti-rabbit Alexa Fluor 594 and goat anti-mouse Alexa Fluor 488 (each 1:1000, Invitrogen, ThermoFisher Scientific) in carrier solution ...
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bioRxiv - Neuroscience 2024Quote: ... The primary antibody was then revealed by incubation for 30 min at 37 °C in the dark with donkey anti-mouse Alexa Fluor-488 (1:250; Thermo Fisher Scientific) diluted in Triton X-100 (0.3% ...
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bioRxiv - Immunology 2022Quote: ... and 10mM HEPES at 4°C for 18h and revealed with fluorochrome-conjugated anti-mouse and IgG1 and IgG2b antibodies (Thermo Fisher Scientific) for 1 hour at room temperature ...
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bioRxiv - Immunology 2023Quote: ... T-cells were incubated overnight at 4 °C with either mAb reactive to mouse phospho-CD247 (CD3 zeta) (Tyr111, Tyr123) (clone EM-55; Thermo Fisher Scientific) or mAb reactive to mouse ZAP70 phosphorylated at position 319 (clone 1503310 ...
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bioRxiv - Genetics 2023Quote: Mouse OP9-K cells were grown at 37°C and 5% CO2 in OP9 propagation media: MEM-α (Life Technologies #12571-063), supplemented with 20% FBS (Sigma-Aldrich #F4135) ...