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Citations for Thermo Fisher :
2201 - 2250 of 10000+ citations for Mouse Presenilin Enhancer 2 Homolog C. Elegans PSENEN ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Mouse body temperature was maintained at 37°C using a warming pad connecting to a water heating system (Thermo Scientific, Waltham, USA). Respiration was continuously monitored using an MRI-compatible monitor system (SA Instruments ...
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bioRxiv - Neuroscience 2023Quote: ... They were then incubated at 4 °C overnight with primary antibody rabbit anti-Myosin VI (1:400 Proteus Bioscience) and mouse anti-green fluorescent protein (Invitrogen, cat. A11120). The tissues were incubated with 1:800 diluted secondary antibodies for 2 hrs at room temperature ...
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bioRxiv - Bioengineering 2023Quote: ... at 4°C overnight and then alkaline phosphatase (AP) conjugated anti-mouse IgG adsorbed with rat serum protein) (Thermo Fisher Scientific, USA) for 2 h diluted 1 ...
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bioRxiv - Cell Biology 2023Quote: ... primary antibodies in 10% GS in 1xPBS overnight at 4°C followed by a 1h incubation with anti-mouse (A11003, Invitrogen, 1:800) secondary antibody in 10% GS in 1xPBS at room temperature ...
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bioRxiv - Genetics 2023Quote: ... before being incubated at 4°C in Odyssey Blocking Buffer (PBS) containing mouse anti-V5 antibody (RRID: AB 2556564, R960-25; Thermo Fisher Scientific) at a 1:3000 dilution for detection of 3v5 tagged alleles of Ume6 ...
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bioRxiv - Immunology 2023Quote: ... Intracellular cytokines were stained at 4°C overnight with FITC or PerCP-e710-conjugated rat anti-mouse IL-1β (NJTEN3; Thermo Fisher Scientific) and PE-conjugated Armenian hamster anti-mouse IL-1α (ALF-161 ...
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bioRxiv - Neuroscience 2024Quote: ... neurons were incubated with primary antibodies (Rabbit-anti-GFP: 1:1000, Thermo Fisher Scientific, Cat. #G10362; mouse-anti-c-myc: 1:200, Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were washed in PBS three times for 5 min at 4 °C by rocking and then incubated by rocking with secondary antibody (Goat-anti-Mouse 488, 1:2000, Invitrogen #A-11008) in 1% BSA PBST for 1 hour at RT in the dark ...
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bioRxiv - Synthetic Biology 2024Quote: ... Membranes were incubated overnight (50 rpm, 4°C) with either mouse anti-His monoclonal (Thermo Fisher; 1:3000 in 5% skimmed milk) or anti-IFNa2 primary antibody (abcam ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 % SDS) for 30 minutes at 50 °C prior to reprobing using mouse anti-3-phosphoglycerate kinase anti-serum (Invitrogen-Molecular probes, A6457).
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bioRxiv - Molecular Biology 2020Quote: ... 4°C) and RNA was isolated using Trizol® Plus RNA Purification Kit (Ambion, Life Technologies, USA). The quality and quantity of RNA was determined by agarose gel electrophoresis and also using a NanoDrop ND-2000 spectrophotometer ...
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bioRxiv - Cancer Biology 2020Quote: ... c-DNA was synthesised by reverse transcription PCR using Verso cDNA synthesis kit (ThermoFisher Scientific, Cat # AB1453A) from 300 ng of RNA ...
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bioRxiv - Cell Biology 2021Quote: ... Immunostainings were performed by incubating cells with anti-c-Kit (clone 2B8, BD Biosciences or Life Technologies), anti-Sca1 (clone D7 ...
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bioRxiv - Cancer Biology 2020Quote: ... Pierce Trypsin/Lys-C Protease Mix and tandem mass tag (TMT)-labeling kit were from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Cancer Biology 2022Quote: ... c-DNA was synthesised by reverse transcription PCR using Verso cDNA synthesis kit (ThermoFisher Scientific, Cat # AB1453A) from 300 ng of RNA ...
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bioRxiv - Molecular Biology 2020Quote: ... for 45 min at 37 °C and reverse transcribed using the Taqman reverse transcription kit (Applied Biosystems) according to the manufacturer’s protocol ...
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bioRxiv - Physiology 2021Quote: ... heat inactivation at 65 °C and column purification (GeneJet PCR Purification Kit; ThermoFisher Scientific, Waltham, MA, USA). The in vitro transcription of ayRhp1 capped mRNA (cRNA ...
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bioRxiv - Plant Biology 2022Quote: ... the construct was recombined with pB7FWG2 destination vector (YFP in C-terminal) using LR ClonaseMixII kit (Invitrogen) and introduced into Arabidopsis (sbt3.3 ...
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bioRxiv - Microbiology 2024Quote: ... at ™70 °C before extraction using a GeneJET RNA Purification Kit (Cat. no. K0731, Thermo Scientific). cDNA synthesis and Quantitative RT- qPCR was performed as described above using various primer pairs (Table S2) ...
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bioRxiv - Developmental Biology 2024Quote: ... before performing an overnight in vitro transcription reaction at 16°C using the MegaScript IVT kit (Invitrogen). Remaining primers were removed by using EXOI/rSAP-IT (Applied Biosystems ...
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bioRxiv - Molecular Biology 2024Quote: ... for 30 min at 25°C using a Click-iT Cell Reaction Buffer Kit (Thermo Fisher Scientific). After the removal of unreacted azide by MicroSpin G-25 columns (Cytiva) ...
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bioRxiv - Genetics 2023Quote: ... The Hi-C library is quantified using the Qubit DNA high sensitivity kit (Thermo Scientific, cat. Q32851) on a Qubit 2 fluorometer (Thermo Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... at 37°C for 1 hour and was processed with the ChargeSwitch PCR Clean-up Kit (Invitrogen) or Monarch PCR and DNA Cleanup Kit (New England Biolabs).
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bioRxiv - Microbiology 2023Quote: ... c-DNA was synthesized as per protocol in the Superscript III Reverse Transcriptase kit (Invitrogen#18989-093). This was followed by Q-PCR using Sybr green mix (Promega) ...
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bioRxiv - Genetics 2024Quote: ... 80°C for 20 minutes for inactivation and mRNA was synthesized using the T7 MEGAshortscript kit (ThermoFisher).
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bioRxiv - Biochemistry 2024Quote: ... 4°C) and the protein concentration determined by a BCA assay (Pierce BCA Kit, Thermo Fisher Scientific). The human protein extracts were further processed to peptides in the same manner as described above for bacterial protein extracts.
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bioRxiv - Neuroscience 2024Quote: ... EdU click-it assay was performed in organoid cryosections per manufacturer’s instructions (EdU kit Invitrogen, #C-10337) followed by immuno-staining for Ki67 ...
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bioRxiv - Physiology 2021Quote: ... 100 μL of One-step Ultra TMB ELISA substrate (Thermo Fisher Scientific, 34028) was added to each well and incubated for 15 min at RT ...
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bioRxiv - Developmental Biology 2021Quote: ... 100 ml of 1-Step-Ultra TMB-ELISA Substrate (Thermo Fisher, cat#34028) was pipetted to each well and incubated for 30 minutes in dark at room temperature ...
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bioRxiv - Immunology 2021Quote: For polyreactivity ELISAs against human insulin (MilliporeSigma) and dsDNA (Calf Thymus DNA; Invitrogen), plates were coated with 2µg/ml and 50µg/ml ...
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bioRxiv - Immunology 2021Quote: Levels of anti-dsDNA IgG were measured by ELISA using MaxiSorp plates (Nunc) coated with dsDNA from calf thymus (20 µg/ml ...
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bioRxiv - Microbiology 2021Quote: ... were coated onto wells of an uncoated 96-well ELISA plate (Nunc Maxisorp) overnight at 4°C in coating buffer ...
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bioRxiv - Microbiology 2021Quote: Quantification of IL-1β secretion in cell supernatant was measured by ELISA (Invitrogen, IL-1 beta Mouse Uncoated ELISA Kit ...
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bioRxiv - Microbiology 2020Quote: ... 96-well ELISA plates (Nunc Maxisorp or Nunc Immobilizer Amino Plates, ThermoFisher, USA) were incubated with 1.2 μg of each peptide diluted in 100 μl of phosphate-buffered saline (PBS ...
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bioRxiv - Microbiology 2022Quote: ELISA: Immulon 4 HBX high binding clear flat bottom 96 well plates (ThermoFisher) were coated with SARS-CoV2 Spike RBD319-591-Fc fusion at 5 μg/ml in 1xPBS overnight at 4 °C followed by washing ...
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bioRxiv - Biochemistry 2022Quote: ... and 200μl of tetramethylbenzidine (TMB) ELISA substrate (Thermo Fisher Scientific; cat. no. 34028) was added to each well ...
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bioRxiv - Microbiology 2021Quote: ... Colorimetric changes were measured using an ELISA microplate reader (MULTISKAN FC, Thermo Scientific) at 450 nm ...
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bioRxiv - Microbiology 2020Quote: ... and PDGF-D by enzyme-linked immunosorbent assay (ELISA, TGF-β: Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... ELISA plates were developed using 100μl of TMB substrate (Thermo Fisher Cat# 34029). OD 450 reading was measured via BioTek ELISA microplate reader.
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bioRxiv - Microbiology 2022Quote: ... The antigen-antibody reaction was probed by TMB ELISA Substrate Solution (Invitrogen eBioscience) and was terminated with 1M sulfuric acid after 15 minutes ...
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bioRxiv - Immunology 2019Quote: ... and IL-10 were analyzed by ELISA as described by the manufacturer (ThermoFisher).
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bioRxiv - Zoology 2020Quote: ... Results were read using an ELISA plate reader (MultiskanTM- Thermo Fisher Scientific®).
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bioRxiv - Neuroscience 2020Quote: ... and subjected to human total tau ELISA (human tau: # KHB0042, Thermo Fisher Scientific) according to manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: ... Plates were washed and 1-Step Ultra-TMB ELISA Substrate Solution (Thermo Scientific) was added to develop the plates ...
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bioRxiv - Immunology 2020Quote: Quantification of total IgA antibody secretion was measured by sandwich ELISA (Thermo scientific) with minor modifications ...
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bioRxiv - Immunology 2021Quote: ... followed by 100 µL of TMB-ELISA substrate (Thermo Fisher Scientific catalog # 34028) and incubated at room temperature for 3 to 5 min ...
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bioRxiv - Immunology 2021Quote: Plasma CXCL16 levels were measured in duplicate using a commercial ELISA (ThermoFisher Scientific), with a coefficient of variation of less than 10% ...
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bioRxiv - Immunology 2020Quote: Cytokines were measured by capture ELISA according to the manufacturer’s instructions (eBiociences, ThermoFisher). Cytokine concentration in serum samples was expressed as pg/mL ...
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bioRxiv - Microbiology 2021Quote: ... Anti-Env antibodies were immobilized on white MaxiSorp ELISA plates (Thermo Fisher Scientific) at a concentration of 5 μg/ml in 100 μL of PBS overnight at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... The substrate solution was 1-StepTM Ultra TMB-ELISA (Thermo Fisher Scientific, 34028) and the stop solution was 2N sulfuric acid (VWR ...