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Citations for Thermo Fisher :
151 - 200 of 10000+ citations for Piggybac Transposable Element Derived 3 PGBD3 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... and cervical cancer-derived HeLa cells were cultivated in DMEM (ThermoFisher) supplemented with 10% fetal calf serum (FCS ...
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bioRxiv - Physiology 2022Quote: ... Mitochondria-derived ROS were identified with MitoSOX Red™ (Invitrogen, M36008) after 30 min of BPS exposure and quantified by fluorescence spectroscopy (510/580 nm ...
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bioRxiv - Developmental Biology 2023Quote: ... KiPS line was derived by reprogramming of commercial human keratinocytes (Invitrogen) with Sendai viruses encoding for OSKM 62 and kindly provided by Austin Smith’s laboratory ...
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bioRxiv - Genomics 2023Quote: Commercially available human iPSC lines derived from a female donor (Gibco Episomal hiPSC ...
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bioRxiv - Immunology 2023Quote: Adipocytes were differentiated from human adipose-derived stem cells (ADSCs, ThermoFisher), which were obtained from human lipoaspirate tissue and cryopreserved from primary culture ...
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bioRxiv - Bioengineering 2023Quote: RNA from iPSC-derived cardiomyocytes was extracted using TRIzol (ThermoFisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: Gibco® H9 hESC-Derived Human Neural Stem Cells (hNSC; ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... hIPSCs derived NPCs were dissociated with Trypsin-EDTA 0.05% (Thermo Scientific), plated on polyornithine-/laminin-coated (PL ...
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bioRxiv - Immunology 2023Quote: ... Bone marrow-derived dendritic cells (BMDCs) were differentiated in RPMI1640 (Gibco) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Genetics 2023Quote: Bone-marrow-derived mesenchymal stem cells were cultured in MEMa (Gibco) with 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2024Quote: ... Primary organoids (crypt-derived) were cultured in advanced DMEM/F12 (Gibco) supplemented with 5% fetal calf serum (FCS) ...
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bioRxiv - Molecular Biology 2024Quote: were derived from the HEK293 Flp-In™ cell line (Invitrogen). LARP4 KO cells were confirmed to lack LARP4 expression via western blot ...
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bioRxiv - Bioengineering 2024Quote: ... 2.5 ng/mL brain-derived neurotrophic factor (BDNF; Gibco #450-02)37,38 ...
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bioRxiv - Bioengineering 2024Quote: ... Diff medium was composed of Iscove’s Derived Eagle’s medium (IMDM, Invitrogen) supplemented with 20% ESC-screened fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2024Quote: ... This backbone was derived from pcDNA5/FRT/TO vector (Life Technologies) with modified MCS ...
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bioRxiv - Immunology 2022Quote: ... anti-Human CD223(LAG-3) antibody (Invitrogen, eBioscience,, Percp-efluor™710), anti-Human CD45RA antibody (Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: ... After 1–3 h incubation with Alexa Fluor-conjugated secondary antibodies (Invitrogen) followed by DAPI staining (0.1 μg/ml ...
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bioRxiv - Cell Biology 2021Quote: ... A monoclonal antibody directed toward galectin 3(clone A3A12) was from ThermoFisher. A polyclonal antibody that detects galectin 3 (ab53082 ...
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bioRxiv - Developmental Biology 2023Quote: ... coated with 3 µg of HA-Tag monoclonal antibody (#26183, ThermoFisher Scientific) at 4°C for overnight ...
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bioRxiv - Cell Biology 2022Quote: ... Insulin Receptor β CT-3 mouse monoclonal antibody (1:1000, AHR0271, ThermoFisher), Perilipin-1/PLIN1 D1D8 rabbit monoclonal antibody (1:1000 ...
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bioRxiv - Cancer Biology 2022Quote: ... 3 μg/mL anti-CD3 antibody (ThermoFisher Scientific, Cat#16-0038-85), 5 μg/mL anti-CD28 antibody (ThermoFisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... incubated for 2 h at room temperature with the appropriate secondary antibodies (all Donkey-derived from Thermo Fisher or Jackson Immunology using Alexaflours 488, 594, and 647; Life Technologies; 1:1,000), washed in PBS with DAPI (1:20,000 ...
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bioRxiv - Biochemistry 2024Quote: ... 3 mg of protein was subjected to immunoprecipitation using ISG15 antibody (Recombinant rabbit monoclonal antibody: ThermoFisher scientific: 7H29L24). Lysates were incubated for 1 hr with the antibody on a rotor at 4 °C ...
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bioRxiv - Biophysics 2021Quote: ... U2OS cells were first transfected with PiggyBac Tet-On 3G-(ArrayG/N)16X and PBQM-mwGFP plasmids using the Neon Transfection system (Thermo Fisher Scientific) and the following pulse program (pulse voltage ...
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bioRxiv - Cell Biology 2021Quote: ... The sensor plasmid was co-electroporated at a 1:1 ratio with the piggybac transposase plasmid using the Neon electroporation transfection system (Thermo Fisher Scientific). We used pulse voltage 1350 V and pulse width 35 ms.
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bioRxiv - Molecular Biology 2022Quote: ... The plasmid containing GFP-P2A-FLAG-PURA as well as a PiggyBac Helper plasmid (27) were transfected into cells using Lipofectamine 3000 (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... These sequences were cloned into pENTR plasmid vector followed by recombination with piggyBac vector [9] using Gateway LR Clonase II Enzyme Mix (11791-020, Thermo Fisher Scientific). The piggyBac vector and transposon vector were kind gifts from Dr ...
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bioRxiv - Bioengineering 2023Quote: ... were respectively co-transfected with hyperactive piggyBac transposase expression vector (1 μg/well) into HEK293T cells using Lipofectamine 2000 (Thermo Fisher Scientific). Forty-eight hours after transfection ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were transfected with pXLone-dCas9-DNMT3A3L-P2A-EGFP together with a plasmid encoding the piggyBAC transposase using the Lipofectamine 2000 transfection reagent (ThermoFisher, Cat# 11668019), and selected with 6 μg/mL blasticidin (RPI Research Products ...
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bioRxiv - Genetics 2023Quote: ... and transfected at 70% confluency with 225 ng of the PiggyBac-transposon and 25 ng of the transposase using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... were transfected with PB-TA-ERN and piggybac transposase (pRP[Exp]-EGFP-EF1A>hyPBase, VectorBuilder) using lipofectamine 2000 transfection reagent (Thermo Fisher Scientific), according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... coding sequence was cloned into piggyBac plasmid and co-transfected with pCMV-hyPBase vector by electroporation (Neon transfection system, Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2024Quote: ... The CD63/CD81-FPs sequences were then transferred from the pENTR3C plasmid into the piggyBac vector [24] pPV EF1a-GW-iPA using Gateway LR Clonase II Enzyme Mix (11791-020, Thermo Fisher Scientific).
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bioRxiv - Immunology 2024Quote: ... slides were washed with 1:3 diluted blocking buffer (3-times, 1 minute) and incubated with anti-mouse Alexa fluor-594 antibody (Invitrogen, A21203), anti-rabbit Alexa fluor-750 (Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: ... Donkey derived secondary antibodies conjugated with Alexa Fluor-488 or −647 (1:500; Thermo Fisher Scientific; #A-11034) (1:500; Thermo Fisher Scientific; #A-31571) were used for fluorescence ...
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bioRxiv - Cancer Biology 2021Quote: ... adult mouse CM or iPSC-derived CM was prepared using Trizol (Invitrogen), cDNA synthesis was performed with 1 μg of total RNA digested with DNaseI and subjected to SYBR green qRT-PCR (primer sequences are provided in Table 1 ...
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bioRxiv - Immunology 2021Quote: ... Absolute numbers were derived from cell counts using a hemocytometer (Life Technologies) or CountBright Absolute Counting Beads (Invitrogen).
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bioRxiv - Biochemistry 2021Quote: ... was cloned into a custom mammalian expression vector derived from pcDNA3.1 (ThermoFisher). Escape mutations P384H ...
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bioRxiv - Cell Biology 2021Quote: ... Patient-derived GBM cells were cultured in Neurobasal medium (Gibco, Cat# 21103049) supplemented with N2 (Gibco ...
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bioRxiv - Bioengineering 2021Quote: Human-derived cell lines (HEK293FT and HeLa) were purchased from Invitrogen (R70007) and American Type Culture Collection (CCL-2) ...
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bioRxiv - Biochemistry 2020Quote: ... BY4741-derived gene deletion mutants obtained from the Yeast Knockout Collection (Invitrogen) or remade by homologous recombination of PCR-amplified drug resistance genes with flanking homology to the gene of interest (Table S1) ...
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bioRxiv - Biochemistry 2021Quote: Human derived neuroblastoma cells (SH-SY5Y) were cultured in DMEM-F12 (Invitrogen) medium supplemented with 10% (v/v ...
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bioRxiv - Immunology 2022Quote: ... The Spodoptera frugiperda-derived Sf21 cell line (Thermo Fisher Scientific, Waltham, MA) was maintained in Insect-XPRESS cell culture media (Lonza Bioscience ...
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bioRxiv - Cell Biology 2021Quote: ... an additional polyclonal population of biopsy-derived human myoblasts was purchased (Gibco human skeletal myoblasts A12555 ...
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bioRxiv - Immunology 2021Quote: Organoids were propagated by dissociating crypt-derived organoids in TryplE Express (Invitrogen) for 3min at 37C ...
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bioRxiv - Microbiology 2021Quote: ... and one derived from the C-terminal region (SDGGRDEVEKNNGLDVIR, Thermo Fisher, USA) (1:4000 dilution of affinity purified antibody stock of 2.95 mg/ml) ...
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bioRxiv - Biochemistry 2023Quote: iATPSnFR1 was cloned into a bacterial expression vector derived from pRSET (Invitrogen) with restriction sites designed to make downstream subcloning into mammalian expression vectors easier ...
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bioRxiv - Immunology 2023Quote: Human hiPSC-derived microglia were transfected with Lipofectamine RNAiMAX reagent (Life Technologies) according to the manufactureŕs protocol ...
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bioRxiv - Neuroscience 2023Quote: ... Growth factors (10 ng/ml brain derived neurotrophic factors (BDNF, Gibco, #PHC7074); 10 ng/ml Glial derived neurotrophic factor (GDNF ...
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bioRxiv - Immunology 2022Quote: FLT3-derived BMDCs were generated by differentiating bone marrow in IMDM (Gibco), 10% B16-FLT3L derived supernatant ...