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Citations for Thermo Fisher :
51 - 100 of 10000+ citations for Piggybac Transposable Element Derived 3 PGBD3 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Seawater dFe was quantified using an internal standard element (57Fe) with inductively coupled plasma mass spectrometry (Element 2, Thermo Scientific). The mass spectrometer was operated in medium-resolution mode with 4000 resolution (FWHM) ...
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bioRxiv - Immunology 2022Quote: ... Human monocyte-derived macrophages (MDMs) were derived from CD14+ peripheral blood monocytes by culturing cells in RPMI/1640 (Gibco) media containing 10% heat-inactivated human AB serum (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2024Quote: ... 5,000 live cells (or 1,000 live cells for tissue-derived colonoids) were quantified by a Countess™ 3 FL Automated Cell Counter (ThermoFisher Scientific, Massachusetts, USA) and plated in 10 µL Matrigel per well in a 96-well plate with 100 µL of media ...
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bioRxiv - Bioengineering 2020Quote: ... UK) and the piggyBac-BE3-GFP plasmid at a 1:4 molar ratio using Lipofectamine 3000 (Invitrogen). At 48 h after transfection ...
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bioRxiv - Cancer Biology 2023Quote: Functional shRNA was cloned into piggybac transposon inducible expression vector PB_tet-on_Apple_shGFP using HindIII and Kpn2L (ThermoFisher)25 ...
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bioRxiv - Microbiology 2024Quote: ... mCherry-RNF213 was transferred into piggybac vector PB-TA-ERN using LR clonase enzyme (Thermo Fisher Scientific) for dox-inducible expression of mCherry-RNF213 ...
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bioRxiv - Developmental Biology 2024Quote: ... the cDNAs were inserted into piggyBac transposon expression vectors using the Gateway cloning kit (Thermo Fisher 12535029) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... CHIKV cDNA was generated from 10 μl of serum derived RNA or 1 μg of tissue derived RNA using random primers (Invitrogen) with SuperScript IV reverse transcriptase (Life Technologies) ...
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bioRxiv - Genomics 2020Quote: Flp-In T-REx 293 (HEK293-derived; Invitrogen) and HEK293T (American Type Culture Collection ...
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bioRxiv - Immunology 2020Quote: ... target gene mRNA levels were derived from Affymetrix expression microarray data (6).
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bioRxiv - Neuroscience 2020Quote: ... human recombinant brain-derived neurotrophic factor (Life Technologies), penicillin/streptomycin ...
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bioRxiv - Neuroscience 2022Quote: Human derived HEK293 cells (Invitrogen, Inchinnan, UK, R75007) stably transfected with cAMP GloSensor™ (pGloSensor-22F ...
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bioRxiv - Cell Biology 2023Quote: ... and SNP array derived genotypes generated by Affymetrix UK Biobank AxiomTM Array kit by Cambridge Genomic Services (CGS) ...
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bioRxiv - Cancer Biology 2023Quote: ... Adipose-derived MSCs were purchased from Fisher Scientific and grown in MesenPRO media growth supplement with 2% MesenPRO growth supplement ...
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bioRxiv - Cell Biology 2020Quote: ... anti-aggrecan antibody BC-3 was from Thermo Scientific and anti-decorin antibody ab175404 was from Sigma.
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bioRxiv - Cell Biology 2020Quote: ... then incubated for 3 hours in secondary antibodies (Invitrogen) used at a 1:500 dilution ...
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bioRxiv - Cell Biology 2023Quote: ... then incubated for 3 hours in secondary antibodies (Invitrogen) at a 1:500 dilution ...
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bioRxiv - Cell Biology 2022Quote: ... Secondary antibody: goat-derived anti-Rat IgG (H+L) conjugated to Alexa Fluor 488 (AF488) (dilution 1:250; Thermo Fisher). Samples were incubated at room temperature for 1h in the dark ...
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bioRxiv - Plant Biology 2024Quote: ... quantified by HR ICP-MS (Thermo Scientific, Element 2TM). For each sample ...
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bioRxiv - Molecular Biology 2021Quote: ... 3×105 cells were co-transfected with 0.5ug of the different versions piggyBac vectors and 0.5ug of pBroad3_hyPBase_IRES_tagRFPt using Lipofectamine 2000 (Thermo Fisher Scientific, 11668019) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... and total DNA amounts of 5μg (2.5μg donor vector plus 1.25μg of each TALENS arm plasmid) or 2μg (1μg piggybac vector plus 1μg of transposase plasmid) were combined with Lipofectamine Stem (ThermoFisher, STEM00003) in Opti-MEM (ThermoFisher 11058021) ...
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Polarized Mechanosensitive Signaling Domains Protect Arterial Endothelial Cells Against InflammationbioRxiv - Cell Biology 2023Quote: HAECs were transfected with GCaMP plasmid (pPB_CAG_GCamp5g) with PiggyBac construct (PB_Vector) (gift from Dr. Roy Wollman, UCLA) using Lipofectamine 3000 transfection reagent (Invitrogen #L3000001) in Opti-MEM media (Gibco #31985062 ...
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bioRxiv - Neuroscience 2024Quote: Rabbit monoclonal or polyclonal antibodies include those against 14-3-3 (Invitrogen, 51-0700, 1:1000); clathrin heavy chain (Abcam ...
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bioRxiv - Neuroscience 2022Quote: Total RNA was extracted from hiPSC-derived cortical neurons at DIV8 (Q5, Q6, n=3 each group) using the miRVana miRNA isolation kit (#AM1560, Ambion, Thermo Fisher Scientific, Waltham, MA, USA) in accordance to their manuals ...
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bioRxiv - Cancer Biology 2023Quote: ... Patient-derived primary cell line GCDX62 was maintained in a 3:1 mixture of Keratinocyte-SFM (KSF; Thermo Fisher Scientific; supplemented with 2% (v/v) FCS ...
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bioRxiv - Neuroscience 2021Quote: ... iPSC-derived MNs were dissociated using trypsin (Thermo Fisher), embedded in diluted Matrigel (Corning ...
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bioRxiv - Cell Biology 2021Quote: ... iPSC-derived MNs were dissociated using trypsin (Thermo Fisher), embedded in diluted Matrigel (Corning ...
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bioRxiv - Immunology 2021Quote: Bone marrow-derived macrophages were differentiated in DMEM (Gibco) containing 20% 3T3 supernatant (as a source of M-CSF) ...
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bioRxiv - Neuroscience 2023Quote: ... 20 ng/ml brain-derived neurotrophic factor (BDNF, GIBCO), 10 ng/ml Wnt3a (R&D Systems) ...
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bioRxiv - Neuroscience 2023Quote: ... Brain-derived neurotrophic factor (BDNF, 20 ng/ml, Invitrogen) was supplemented to support survival of reprogrammed neurons (Guo et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... antibody was purchased from Generon LTD and rabbit-derived polyclonal antibodies against vinculin (42H89L44) and calnexin (ab22595) were purchased from Thermo Fisher Scientific and Abcam ...
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bioRxiv - Cell Biology 2021Quote: ... antibodies were 3-fold serially diluted in MEM medium (Gibco) from 20 μg/mL for preparing the working solution ...
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bioRxiv - Cell Biology 2022Quote: ... The secondary antibody in PBS supplemented with 3% BSA (Thermofisher, Mouse 800 ...
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bioRxiv - Immunology 2023Quote: ... antibodies for 3 hours and stained for TMRM (Thermo Fisher) and active-Caspase3 (BD Biosciences) ...
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bioRxiv - Developmental Biology 2023Quote: ... and Cyanine 3-conjugated anti-mouse IgG antibody (M30010, Invitrogen). Embryos were washed with PBS for three times and then PBS was replaced with 10% ...
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bioRxiv - Biochemistry 2024Quote: ... and 3 μg/ml anti-HA antibody (Invitrogen, Cat# 26183) for one hour and a half at room temperature ...
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bioRxiv - Developmental Biology 2022Quote: ... and 50 ng of PiggyBac-hgRNA L21 + puro library were combined and added to 48 ul Opti-MEM (Gibco). 2 ul of Lipofectamine Stem Transfection Reagent (Thermo Fisher ...
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bioRxiv - Neuroscience 2024Quote: ... iPSCs were transfected with 4.5 μg of NIL piggyBac construct and 0.5 μg of transposase vector using the Neon Tranfection System (Life Technologies) and then selected with 5 μg/ml blasticidin in Nutristem-XF medium containing 0.1X P/S for 10 days ...
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bioRxiv - Molecular Biology 2024Quote: ... Halo-yNTD-mTBPc) together with 1 μg of the Super Piggybac transposase plasmid using Lipofectamine 2000 (Invitrogen 11668-019) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... The following antibodies were used as primary antibodies: FFAR 3 (1: 100, #PA5-97745, Invitrogen) and GPR 43(1 ...
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bioRxiv - Cancer Biology 2020Quote: ... PCR amplified enhancer elements were cloned into pENTR5’ vectors (ThermoFisher). Anytime Phusion (NEB ...
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bioRxiv - Biophysics 2023Quote: ... together with other salt and trace elements (Thermo Fisher Scientific) as detailed in Supplementary Table 7 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Samples were processed using an element analyser (ThermoFisher, Flash 1112) connected to a continuous-flow inlet of a gas source mass spectrometer (ThermoFisher ...
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bioRxiv - Neuroscience 2024Quote: ... The ICP-sf-MS (“ELEMENT 2”, Thermo Scientific, Bremen, Germany) settings included a radio frequency power of 1260 W ...
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bioRxiv - Neuroscience 2022Quote: ... They were stained for 2 days at 4°C with a mouse-derived antibody against GFP (1:400, mAB 3E6, Invitrogen, Darmstadt, Germany), washed for 2 hours at RT in 1x PBS ...
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bioRxiv - Neuroscience 2023Quote: ... The secondary antibodies used for hiPSC-derived neurons and microglia were as follows: anti-rabbit or anti-mouse antibodies conjugated with Alexa Fluor 488 (Invitrogen, 1:1000), anti-rabbit or anti-mouse antibodies conjugated with Alexa Fluor 555 (Invitrogen ...
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bioRxiv - Cancer Biology 2020Quote: ... cells from the previous steps were barcoded by cotransfecting PB-EF1α-Puro-V8.2 library and Super PiggyBac Transposase plasmid (SBI #PB210PA-1) at a 1:10 molar ratio using Lipofectamine 3000 (Thermofisher). Barcoded cells were puromycin-selected for 7 d ...
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bioRxiv - Microbiology 2020Quote: ... cells were co-transfected with EYFP plasmids harboring the piggyBac transposon (pPB bsr2-EYFP) (Yusa et al., 2009) and pCMV-mPBase (mammalian codon-optimized PBase) encoding a piggyBac transposase using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: Gene editing of pig fibroblast cells were performed by electroporation of wildtype large white pig fibroblast cells with a plasmid encoding Piggybac transposase and pBv1-EF-6X plasmid using Neon Transfection System (Invitrogen) under condition of 1450 V ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 x 105 ESCs were co-transfected with 1.5 μg wild-type or mutants of PB-ATRX-HA-Neo and 1.5 μg Super PiggyBac transposase using Lipofectamine 3000 (Thermo Fisher). After two days of transfection ...