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Citations for Thermo Fisher :
101 - 150 of 10000+ citations for Piggybac Transposable Element Derived 3 PGBD3 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Mito-Dendra2 was a gift from David Chan.The Mito-Dendra2 sequence was inserted to a PiggyBac backbone construct along with a T2A Neomycin selection cassette using the GeneArt assembly kit (Invitrogen). We also used a pgK-Puro-CMV-GFP and pPb-CAG-RFP-Hygro construct cloned in the lab and a PiggyBac vector containing the transposase.
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bioRxiv - Cell Biology 2020Quote: The PiggyBac EC-BioID-GFP and C-BioID-EGFP plasmids were transfected into Ecad-KO cells [47] using Lipofectamine2000 (Invitrogen) along with PiggyBac Transposase expression plasmid (System Biosciences ...
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bioRxiv - Molecular Biology 2022Quote: ... Stably expressing cell-lines were generated by transfecting 1 μg of the HaloTagged TF Piggybac construct together with 1 μg of the Super Piggybac transposase plasmid (gifted from the Tjian Lab) using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... These entry clones were recombined with a piggyBac vector (a gift from K. Woltjen) 10 by using the Multisite Gateway technology (Invitrogen). The constructs were stably introduced into the cells with by electroporation with Amaxa Nucleofector (Lonza ...
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bioRxiv - Immunology 2024Quote: ... a cell line model for TNBC) was stably transfected with PiggyBac ROPN1/B-GFP DNA using Lipofectamine P3000 transfection reagent kit (Invitrogen) and Transposase Expression vector DNA (System Biosciences) ...
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bioRxiv - Neuroscience 2024Quote: ... cells were co-transfected with 4.5 µg of transposable vector epB-Puro-TT-SYN1-HuD (SYN1::HuD) and 0.5 µg of the piggyBac transposase using the Neon Transfection System (Life Technologies) as described (Garone et al. ...
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bioRxiv - Developmental Biology 2023Quote: ... into a vector backbone containing PiggyBac transposition sites.35 The Cer1:H2B-Venus reporter construct was co-transfected with CAG-pBASE35 using Lipofectamine 2000 (ThermoFisher) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were co-transfected with pXLone-dCas9-DNMT3A3L-P2A-EGFP and different pKH011-puro guide plasmids together with the piggyBAC transposase plasmid via electroporation with the Neon Transfection System (ThermoFisher). Transfected cells were selected and maintained with 6 μg/mL Blasticidin and 6 μg/mL Puromycin ...
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bioRxiv - Developmental Biology 2023Quote: ... A total of 800 ng of purified DNA (400ng of expression vector and 400 ng of the vector carrying the PiggyBac transposase (kind gift form J. Betschinger, Novartis)) were diluted in 50 µl of OptiMEM (Catalog no. 31985062, Gibco)) ...
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bioRxiv - Genomics 2023Quote: ... approximately 1.5 million wildtype MCF10A cells were incubated with 1 µg PiggyBac-NLS-ZapCV2 and 200 ng Super PiggyBac Transposase (Systems Biosciences #PB210PA-1) and electroporated using a Neon Electroporation System (ThermoFisher). Cells were plated into antibiotic-free media (the same composition as above ...
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bioRxiv - Developmental Biology 2024Quote: ... These entry clones were recombined with the piggyBac vector42 (a gift from K. Woltjen) by using the Multisite Gateway technology (Invitrogen). The constructs were stably introduced into the human iPSCs and mouse EpiSCs by electroporation with a 4D Nucleofector (Lonza ...
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bioRxiv - Bioengineering 2024Quote: ... K562 cells were co-transfected with Piggybac plasmid encoding both pTREtight-dCas9 and pEF1a-BlastR-P2A-rTA3 (without transposases) and PiggyBac transposase plasmid using Lipofectamine 2000 (Invitrogen). Two days following transfection ...
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bioRxiv - Cell Biology 2024Quote: ... and a geneticin resistance marker (0.5 μg) and Super PiggyBac transposase expression vector (0.2 μg) using Lipofectamine 2000 (Thermo Fisher Scientific). Clones were selected using geneticin resistance (500 μg/ml ...
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bioRxiv - Neuroscience 2020Quote: Glial-Derived Neurotrophic Factor (GDNF) Recombinant Human Protein (ThermoFisher, #PHC7044)
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bioRxiv - Neuroscience 2020Quote: Brain-Derived Neurotrophic Factor (BDNF) Recombinant Human Protein (ThermoFisher, #PHC7074)
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bioRxiv - Biochemistry 2020Quote: ... Trypsin-EDTA and anhydrous dimethylsulfoxide (DMSO) derived from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Developmental Biology 2022Quote: ... KiPS line was derived by reprogramming of human keratinocytes10 (Invitrogen) with Sendai viruses encoding for OSKM and kindly provided by Austin Smith’s laboratory.
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bioRxiv - Cancer Biology 2021Quote: ... Blood cell-derived cell lines were grown in RPMI (Invitrogen) supplemented with 10% foetal calf serum (FCS) ...
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bioRxiv - Cancer Biology 2021Quote: ... Monocyte-derived human macrophage cultures were maintained in IMDM (Gibco) supplemented with 10% human AB serum and polarized by incubation with 0.5ng/ml of macrophage colony-stimulating factor for 48 hours (MCSF ...
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bioRxiv - Neuroscience 2023Quote: ... 10 ng/ml Glial derived neurotrophic factor (GDNF, Gibco, #PHC7045); 1 mM Dibutyryl cyclic-AMP (Sigma ...
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bioRxiv - Plant Biology 2022Quote: ... cDNA derived from this RNA was synthesised using MMLV (Thermofisher) and an oligo-dT primer ...
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bioRxiv - Developmental Biology 2023Quote: ... KiPS line was derived by reprogramming of human keratinocytes (Invitrogen) with Sendai viruses encoding for OSKM and kindly provided by Austin Smith’s laboratory.
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bioRxiv - Immunology 2023Quote: Patient-derived melanoma cell lines were cultured in RPMI (Gibco) supplemented with 8% fetal calf serum (FCS ...
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bioRxiv - Microbiology 2023Quote: ... Human (H9) embryonic stem cells-derived hNSCs (Thermo Fisher Scientific) were cultured in KnockOut™ D-MEM/F-12 media (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2023Quote: ... iPSC-derived neurons were transfected with Lipofectamine Stem (Thermo Fisher) and 1-2.5 μg total plasmid DNA ...
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bioRxiv - Microbiology 2024Quote: ... Bacteroides-derived lipids were detected with AlexaFluor 647 azide (ThermoFisher) using a Click & Go kit (Vector Laboratories ...
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bioRxiv - Cell Biology 2024Quote: ... 5 μM platelet-derived growth factor-AB (PDGF-AB) (GIBCO), 10 ng/ml tumor necrosis factor-α (TNF-α ...
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bioRxiv - Physiology 2023Quote: ... 1.2 μg of anti-RYR1 monoclonal antibody (ThermoFisher MA 3-925,) was added to the precleared extracts and incubated 1h at 4C ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... a mouse anti-beta 3 tubulin antibody (Invitrogen, MA1-118,1:200) was used.
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bioRxiv - Immunology 2023Quote: ... Cy-3 conjugated anti-rabbit secondary antibody (Invitrogen, 1:300 dilution) was added and incubated for 0.5 hr ...
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bioRxiv - Immunology 2021Quote: ... connected by a porcine teschovirus-1 2A element (“P2A”; Life technologies), recognize the Msln406-414 epitope presented in H2-Db ...
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bioRxiv - Immunology 2020Quote: ... codon-optimized DNA encoding B7-H3 specific scFv derived from the m276 monoclonal antibody (42) was synthesized by GeneArt (Thermo Fisher, Waltham, MA). The scFv was then ligated using infusion cloning (Takara Bio ...
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bioRxiv - Genomics 2023Quote: ... The human origin of PDX-derived cell lines was validated by flow cytometry using FITC anti-human EpCAM antibody clone VU-1D9 and (Thermo Fisher Scientific, #A15755) and PE/Cy7 anti-mouse H2Kd antibody clone SF1-1.1 (Biolegend ...
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bioRxiv - Cancer Biology 2023Quote: ... APCs and patient-derived glioma cell lines were stained with HLA-A2 monoclonal antibody (Clone BB7.2, Thermo Fisher Scientific Cat. #17-9876-42). Approximately 1x106 cells per 100 μL FACS buffer (PBS supplemented with 1% BSA (Sigma Aldrich Cat ...
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bioRxiv - Molecular Biology 2022Quote: ... 3-4 mg total protein and 2 ug of KIF1C antibody or 10 ug mCherry antibody (Invitrogen mCherry Monoclonal Antibody (16D7)) were used ...
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bioRxiv - Developmental Biology 2021Quote: ... E14Tg2a-rtTAms2 cells were then transfected with piggyBac-TetON-Nanog-PGK-Hph-pA or piggyBac-TetON-Esrrb-PGK-Hph-pA together with pCMV-hyPBASE (Yusa et al., 2011) plasmids using lipofectamine 3000 (Thermofisher; L3000015).
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bioRxiv - Biochemistry 2020Quote: ... and 0.5 µg of the piggyBac transposase plasmid pCyL43 (16) were co-transfected to each well using the Lipofectamine 2000 reagent (Thermo Fisher). These cells were then selected using 2 µg/mL puromycin and 200 µg/mL G418 for two weeks ...
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bioRxiv - Genomics 2022Quote: ... The human urothelial bladder tumor cells were transfected with the doxycycline-inducible GFP-tagged APOBEC3G vector and the PiggyBac transposase vectors with the Lipofectamine2000 transfection agent (ThermoFisher Scientific). G418 was used for the selection of the clones with stable genomic insertion of the GFP-tagged APOBEC3G component ...
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bioRxiv - Cell Biology 2023Quote: ... or 0.5 µg rtTA-puro and 1 µg of PiggyBac (for control rtTA cells) in 100 µl Opti-MEM solution (31985062, Life Technologies) containing 5 µl of X-tremeGENE 9 transfection reagent (XTG9-RO Roche ...
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bioRxiv - Synthetic Biology 2024Quote: ... then co-transfected with 400 ng of the constructs to be integrated and 100 ng of the piggyBac transposase together with 2 μl of Lipofectamine 2000 (Invitrogen, 11668027) according to the manufacturer’s instruction ...
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bioRxiv - Neuroscience 2024Quote: ... Brain sections were washed with 0.3% TX-100/PBS 3 times and then incubated with secondary antibody goat anti-rabbit IgG AlexaFluor 555 (A21528, Invitrogen) at room temperature for 2 hours ...
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bioRxiv - Cell Biology 2020Quote: THP1-derived macrophages were incubated in Mg2+-free DMEM (ThermoFisher Scientific) supplemented with 4 μM Mag-Fluo-4 (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... and 20 ng/mL Platelet-derived growth factor (PDGF, Invitrogen, USA) for 3 days at 37 °C under a humidified atmosphere containing 5% CO2 ...
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bioRxiv - Microbiology 2020Quote: ... NGS-derived mAbs were transiently expressed in ExpiCHO cells (Thermo Fisher) with equal amount of heavy and κ-light chain plasmids and purified from culture supernatants after 12-14 days using Protein A beads columns (Thermo Fisher).
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bioRxiv - Immunology 2021Quote: Expi293F cells are derived from the HEK293F cell line (Life Technologies). Expi293F cells were grown in Expi293 Expression Medium (Life Technologies) ...
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bioRxiv - Molecular Biology 2021Quote: ... Expi293F cells are derived from the HEK293F cell line (Life Technologies). Expi293F cells were grown in Expi293 Expression Medium (Life Technologies) ...
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bioRxiv - Neuroscience 2022Quote: Gibco® H9 hESC-Derived Human Neural Stem Cells (hNSC; ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... hIPSCs derived NPCs were dissociated with Trypsin-EDTA 0.05% (Thermo Scientific), plated on polyornithine-/laminin-coated (PL ...
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bioRxiv - Physiology 2022Quote: ... Mitochondria-derived ROS were identified with MitoSOX Red™ (Invitrogen, M36008) after 30 min of BPS exposure and quantified by fluorescence spectroscopy (510/580 nm ...
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bioRxiv - Bioengineering 2023Quote: RNA from iPSC-derived cardiomyocytes was extracted using TRIzol (ThermoFisher Scientific) according to the manufacturer’s protocol ...