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Citations for Thermo Fisher :
1701 - 1750 of 10000+ citations for rno mir 143 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... qRT-PCR was performed using commercially available Taqman primer/probes for cd5 and ubc (Thermo Fisher).
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bioRxiv - Microbiology 2019Quote: ... qPCR was performed using specific primers and the SYBR Green PCR master mix (Applied Biosystems, USA) in a 20 μl reaction system ...
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bioRxiv - Genetics 2019Quote: ... and 0.2 µM each primer in a 7500 Fast Real-Time PCR System (Applied Biosystems, USA). RT-qPCR conditions were as follows ...
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bioRxiv - Immunology 2021Quote: ... and TaqMan Primer/Probes was run on QuantStudio 7 Flex Real-Time PCR System (Thermo Fisher) with standard settings ...
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bioRxiv - Microbiology 2021Quote: ... Quantitative PCR was performed with target-specific primers using the TaqMan Gene Expression Assay (Applied Biosystems) or the Fast SYBR Green Assay (ThermoFisher ...
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bioRxiv - Cell Biology 2021Quote: ... and the primers listed in table S4 on a Quantastudio Real-time PCR instrument (Applied Biosystems). ☐-actin levels were used to normalize input amounts ...
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bioRxiv - Immunology 2021Quote: ... Primers and diluted cDNA samples were prepared with Power SYBR Green PCR Master Mix (Applied Biosystems). Amplification cycles using the iQ5 system (BioRad ...
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bioRxiv - Biochemistry 2020Quote: ... SuperScript® III First-Strand Sythesis SuperMix for qRT-PCR and primers were purchased from Invitrogen, Carlsbad.
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bioRxiv - Cell Biology 2021Quote: ... The following primers were used for amplification on a StepOne Real Time PCR system (Applied Biosystems) (OPTN ...
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bioRxiv - Systems Biology 2021Quote: ... Primers were resuspended in 15μL PCR master mix (0.2μL Phusion High Fidelity DNA Polymerase (Thermo Scientific), 0.4μL 10 mM dNTP Solution (New England Biolabs) ...
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bioRxiv - Microbiology 2021Quote: ... cDNA was subjected to PCR using SYBR Select Master Mix using gene specific primers (Applied Biosystems). Fold increases in gene expression with respect to control samples (indicated in each figure legend ...
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bioRxiv - Microbiology 2019Quote: ... cDNA was subjected to PCR using SYBR Select Master Mix using gene specific primers (Applied Biosystems). Fold increases in gene expression with respect to control samples (indicated in each figure legend ...
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bioRxiv - Immunology 2021Quote: ... 0.4 mM of each primer forward and SYBR Green PCR Master Mix (4309155E, Applied Biosystems Ins.). All quantification reactions were controlled with a melting curve and primer efficiency was tested with standard curves ...
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bioRxiv - Cancer Biology 2022Quote: ... GFP and mCherry expression was assessed using specific primers(39) and SybrGreen PCR reagents (Applied Biosystems). Quantitative real-time PCR (QRT-PCR ...
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bioRxiv - Immunology 2020Quote: ... with specific primers (Supplementary Table S1) in an ABI 7300 Real Time PCR System (Applied Biosystems). Results were analyzed using SDS2.4 software.
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bioRxiv - Neuroscience 2020Quote: ... 0.5 µL Illumina PCR Primer Cocktail and 4.5 µL of 0.6x 2xSYBR green I (Invitrogen, S7563). From the results of this qPCR we determined that each library required 4-5 additional PCR cycles in order to achieve optimal amplification without compromising library complexity ...
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bioRxiv - Cell Biology 2022Quote: ... the primers listed in Supplementary Table 11 and a 7500 Real Time PCR System (Applied Biosystems). Gene expression levels were quantified relative to GAPDH expression using the ΔCt method ...
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bioRxiv - Plant Biology 2019Quote: ... and gene-specific primers (Table S2) on a StepOne Plus Real Time PCR machine (Life Technologies).
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bioRxiv - Immunology 2019Quote: ... 0.4 mM of each primer forward and SYBR Green PCR Master Mix (4309155E, Applied Biosystems Ins.) All quantification reactions were controlled with a melting curve and primer efficiency was tested with standard curves ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 μl of qPCR primer and 10 μl of SYBR® Green PCR reagents (Life Technologies) were added to each well ...
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bioRxiv - Molecular Biology 2022Quote: ... For Gibson Assembly pSRK plasmid was PCR-amplified with primers pSRK_GA_F and pSRK_GA_R and treated with DpnI restriction endonuclease (ThermoFisher).
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bioRxiv - Plant Biology 2023Quote: ... qRT-PCR was performed using gene-specific primers (Supplementary Table 1) and SYBR Green (Life Technologies) on a Bio-Rad iQ5 PCR machine by following the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2024Quote: ... Primer sequences are listed below and the SYBR Green PCR Master Mix (Applied Biosystems, Cat. 4300155) was used ...
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bioRxiv - Plant Biology 2019Quote: ... qRT-PCR amplification was performed using the specific primer pairs (Table S12) on a 7500 real time PCR system (Applied Biosystems Inc., USA). The amplification process was performed according the method of Suo et al ...
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bioRxiv - Evolutionary Biology 2022Quote: ... through PCR amplification using pair of primers 2 and 3 (Table S1) with a 2X Phusion Flash PCR Master Mix (Thermo Scientific, Cat. # F548S). Gibson Assembly (New England Biolabs ...
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bioRxiv - Cancer Biology 2022Quote: ... and gene specific primers were used for PCR amplification and detection on a 7500 FAST Real-Time PCR System (Applied Biosystems, Thermo Fisher Scientific). Relative mRNA levels were determined by comparing the PCR cycle thresholds between cDNA of a specific gene and beta actin for mouse or histones for human (ΔCt).
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bioRxiv - Molecular Biology 2022Quote: ... 5μl of diluted cDNA was used for Real-Time PCR using appropriate primers and SYBR Green Real-Time PCR Master Mix (Thermo Fisher Scientific, USA) in ABI 7500 Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Cancer Biology 2022Quote: ... The tRNA library was PCR amplified for 10 cycles using Illumina multiplex and barcode PCR primers and Platinum SuperFi II DNA Polymerase (ThermoFisher Scientific, Cat #12369002). All oligonucleotide primers and adapters utilized in the procedure are listed in Supplementary Table 3 ...
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bioRxiv - Plant Biology 2022Quote: ... The resultant cDNA was used as template for real-time PCR (primers listed in Supplemental Table 2) using StepOne™ Real-Time PCR System (Applied Biosystems™). Data were processed using the dCt method ...
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bioRxiv - Cell Biology 2023Quote: ... was performed on 12 ng cDNA with SybrGreen PCR mastermix (LifeTechnologies) and specific primers (supplementary Table 2) and run in an ABI? real-time PCR machine (Applied Biosystems, ThermoFisher Scientific). The raw data was analysed through -ΔCt as described in Supplementary Methods.
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bioRxiv - Bioengineering 2019Quote: ... 100 ng of RNA was reversed transcribed using universal RT primers and the TaqMan Advanced microRNA cDNA Synthesis Kit (Thermo Fisher Scientific), according to vendor’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... RT-qPCR was performed with gene specific primers (Additional File 2: Table S1) using Fast SYBR™ Green Master Mix (ThermoFisher Scientific). Reactions were run using the Quant Studio 7 Real-Time PCR System (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... in 15 ml reaction mixture was converted into cDNA using RT primers (50 nM) that were complementary to each miRNA with a TaqMan MicroRNA Reverse Transcription Kit (Life Technologies #4366596). Primers were adapted from (Lee et al. ...
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bioRxiv - Molecular Biology 2019Quote: ... First-strand cDNA was pre-amplified for 14 cycles with the pool of primers used for RT-qPCR and the Taq-Man PreAmplification Master Mix (Life Technologies, 4488593). Primer sequences are in Additional file 5 ...
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bioRxiv - Microbiology 2019Quote: RNA extracted from virus pellets (containing partially purified virus from allantoic fluid pooled from two independent experiments) was reverse transcribed (RT) using the Uni12 primer with the Verso® cDNA kit (Life Technologies), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... RNA samples were reverse transcribed with first strand synthesis primed with RT-primer 1 to 3 with a Maxima H Minus First Strand cDNA synthesis kit (Thermo Scientific, USA) following the manufacturer’s instructions ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... First strand cDNA synthesis was carried out using the RT Random Primers of the High-Capacity cDNA Reverse Transcription kit (Applied Biosystems™) and treated with RNase Inhibitor (Applied Biosystems™ ...
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bioRxiv - Developmental Biology 2022Quote: ... to retain ≥ 200-nt RNAs and reverse transcribed in 25 μl with 50 pmol RT primer (GCA CCC GAG AAT TCC ANN NNN NNN) using SuperScript III (Thermo Fisher, 18080093). After purification with 50 μl Ampure XP beads (Beckman Coulter ...
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bioRxiv - Immunology 2022Quote: ... and hybridized first using RT primer (5′/5Biosg/AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTVN-3′) and then reverse transcribed into cDNA using TSO primer (5′-AAGCAGTGGTATCAACGCAGAGTACATrGrGrG-3’) and RT maxima reverse transcription (Thermo Fisher Scientific). cDNA was amplified using ISPCR primer (5′-AAGCAGTGGTATCAACGCAGAGT-3′ ...
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bioRxiv - Plant Biology 2022Quote: ... stem-loop primers were designed and cDNA for individual miRNA was prepared by pulsed-RT reaction using SuperScript III Reverse Transcriptase (Thermo Fisher Scientific) (Chen et al. ...
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bioRxiv - Microbiology 2023Quote: ... The first-strand cDNA was obtained using oligo(dT) primers and Moloney murine leukemia virus-reverse transcriptase (MMLV-RT kit; Life Technologies, USA), using 100 ng of purified RNA ...
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bioRxiv - Microbiology 2023Quote: ... the first-strand human cDNA was obtained using oligo(dT) primers and Moloney murine leukemia virus-reverse transcriptase (MMLV-RT kit; Life Technologies, USA), and The SuperScript™ VILO™ cDNA Synthesis Kit ...
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bioRxiv - Plant Biology 2023Quote: ... followed by reverse transcription using the RT Oligo primer (Supplementary Data 12 and SuperScript III. cDNAs were subsequently purified using the DynaBeads MyONE Silane system (Thermo Fisher, 37002D), and a second adapter L#clip2.0 (Supplementary Data 12 ...
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bioRxiv - Cell Biology 2023Quote: ... First-strand cDNA was synthesized using a ratio of 2:1 random primers:oligo (dT) primer and SuperScript II RT (Invitrogen, Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... first-strand cDNA was pre-amplified for 14 cycles with the pool of primers used for the RT-qPCR analysis and the Taq-Man PreAmplification Master Mix (Life Technologies, 4488593). RT-qPCRs were then performed and validated on Fluidigm 96.96 Dynamic Arrays using the Biomark HD system (Fluidigm Corp. ...
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bioRxiv - Molecular Biology 2024Quote: ... First-strand cDNA was synthesized using a ratio of 2:1 random primers:oligo (dT) primer and SuperScript II RT (Invitrogen, Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... and DNase Set (Invitrogen, 12185010) as per manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: Factor Staining Buffer Set (Thermofisher), following supplier’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... and DNase set (12185010; Invitrogen) according to the manufacturers’ protocols ...
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bioRxiv - Neuroscience 2021Quote: ... or miR-544-3p inhibitor (50 nM; Thermo Fisher Scientific, Waltham, MA), or negative control mimic (50 nM ...