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Citations for Thermo Fisher :
1701 - 1750 of 2644 citations for 8 Bromoguanosine hydrate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: Circular mtDNA was first linearized by adding 12.5 U/μL MssI enzyme (Pmel, 8-bp endonuclease) (ThermoFisher, MA, USA) for 2 h at 37 °C in accordance with the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: Colons and small intestines (without caecum) from female mice (8-20wks) were isolated and transferred into RPMI-1640 (Gibco) medium supplemented with L-glutamine ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were washed 8-10 times in PBS for 5 minutes and incubated with DAPI (0.1 mg/ml in PBS; #62248, Invitrogen) to stain nuclei ...
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bioRxiv - Neuroscience 2024Quote: ... were transfected into HepaCam enriched astrocytes seeded in 8-well chamber slides using the lipofectamine LTX reagent (0.6μl) and plus reagent (0.2μl, Thermo Fisher Scientific, A1262) containing 40μl Opti-MEM following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: Frozen samples (n=8) were OCT embedded and sectioned at 10μm on a Cryostar NX70 cryostat (Thermo Fisher Scientific). Capture sections were fixed with methanol ...
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bioRxiv - Cell Biology 2024Quote: ... Nuclei were pelleted at 800g for 10 min at 4°C and washed twice in buffer 1 and once in freezing buffer (1M Tris pH 8, 100% glycerol, 100mM MgAc2, 0.5M EDTA, 1M DTT, 1x PTase inhibitor cocktail Roche, 20U/µl SUPERase In Ambion). Nuclei were resuspended in freezing buffer ...
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bioRxiv - Biochemistry 2024Quote: ... 50 μL reactions were prepared with 8 nM SWR1C mixed with 0.5 μM phosphate sensor (fluorophore-conjugated phosphate binding protein, ThermoFisher) in remodeling buffer ...
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bioRxiv - Cell Biology 2023Quote: ... coverslips with the gel stuck to it were transferred to a 6-well plate with 1 ml of digestion solution (0.5 % Triton X100, 1 mM EDTA, pH 8 (AM9260G, Ambion), 50 mM Tris ...
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bioRxiv - Microbiology 2020Quote: Human ileocecal adenocarcinoma HCT-8 cells (ATCC) and THP-1 cells (ATCC) were maintained in Advanced RPMI 1640 (ThermoFisher Scientific) supplemented with 10% fetal bovine serum and 1X penicillin-streptomycin (ThermoFisher Scientific ...
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bioRxiv - Plant Biology 2019Quote: For the co-immunoprecipitation (CoIP) 8 μl affinity-purified anti-CP31A antibody (Kupsch et al., 2012) was bound to 50 μl Dynabeads ProteinG (Invitrogen) under rotation (15 rpm) ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell extracts (20 µg/lane) were fractionated in 3%-8% or 4-12 % tris-acetate gel (NP0006, NuPAGE Life Technologies). All antibodies were used at the concentrations recommended by the manufacturers.
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bioRxiv - Cell Biology 2020Quote: ... the strips were washed 3 times in PBS-T before incubation in primary antibody (GST Tag Mouse anti-Tag, Clone: 8-326, Invitrogen) for 1 h at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... 3.2 μg of p0G44 (pSB2380) and 1 μg of pSB2860/pSB2863 were mixed with 8 μL of Lipofectamine2000 (Invitrogen 11668027) in 400 μL of DMEM (no supplements ...
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bioRxiv - Cell Biology 2020Quote: ... IP and lysate samples were separated by SDS-PAGE on precast 3-8% tris-acetate and 4-12% bis-tris acrylamide gels (Invitrogen) followed by transfer to 0.2 μm nitrocellulose membranes (Amersham) ...
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bioRxiv - Cell Biology 2020Quote: ... larger regions of Lysozyme-immunostained ileal sections (4-8 mm for each animal) were scanned with an EVOS system (Invitrogen) and the total number of Lysozyme-positive epithelial cells per linear ileal length was manually quantified using FIJI ...
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bioRxiv - Developmental Biology 2021Quote: ... All cell lines were cultured routinely in feeder-free conditions in either Essential 8 (Thermo Fisher or made in-house) or mTeSR1 (Stem Cell Technologies ...
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bioRxiv - Genetics 2020Quote: ... Samples were loaded on 4-12% NuPAGE Bis-Tris gels or 3-8% NuPAGE Tris acetate gels (Thermo Fisher Scientific) and transferred to the nitrocellulose membranes (GE Healthcare ...
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bioRxiv - Biophysics 2020Quote: ... USA) for 10 minutes and glued to the bottom of an 8-well chamber (Nunc Lab-Tek, Thermo Scientific, USA) using duplicating silicone (Twinsil soft 18 ...
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bioRxiv - Biophysics 2020Quote: ... USA) for 10 minutes and glued to the bottom of an 8-well chamber (Nunc Lab-Tek, Thermo Scientific, USA) using duplicating silicone (Twinsil soft 18 ...
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bioRxiv - Molecular Biology 2021Quote: 1×104 A549 cells were grown in poly(D-lysine)-coated borosilicate glass Lab-Tek 8-well chambers (Thermo Scientific) and stained with MitoTracker Green FM (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... The two pAcgp67-RBD (residues 333–530) plasmid with a C-terminal 8×His tag were transfected into Sf9 cells using Cellfectin II Reagent (Invitrogen) to produce the recombinant baculoviruses ...
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bioRxiv - Molecular Biology 2021Quote: ... HEK-hACE2 cells were cultured in DMEM with 10% FBS (Hyclone) and 1% PenStrep with 8% CO2 in a 37C incubator (ThermoFisher). Prior to plating ...
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bioRxiv - Molecular Biology 2020Quote: ... Beads were collected by centrifugation and the supernatant loaded onto a Novex NuPAGE Bis-Tris 8% gel (Invitrogen, Carlsbad, CA). Proteins were separated at 175 V for 3-4 hours at 4°C and then transferred to nitrocellulose membrane (BioRad ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates were boiled at 95 °C for 5 min and separated by SDS–PAGE electrophoresis using 8-16% Tris-Glycine gels (Invitrogen) at 225 V for 30 min ...
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bioRxiv - Cell Biology 2022Quote: ... or 1 μg of Super8-FOPflash (8 mutant TCF-binding sites) reporter plasmids (kindly provided by Randall Moon, University of Washington, Seattle) using Lipofectamine 2000 (Invitrogen); a thymidine kinase-Renilla plasmid (0.1 μg ...
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bioRxiv - Immunology 2021Quote: ... 1% PenStrep and 8 μg/mL puromycin (to ensure retention of TMPRSS2) with 5% CO2 in a 37°C incubator (ThermoFisher). Cells were trypsinized using 0.05% trypsin and plated to 40,000 cells/well ...
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bioRxiv - Immunology 2021Quote: ... The Matrigel was allowed to solidify for 8-15 minutes in a 37C incubator and solidified domes were cultured in Advanced DMEM (Gibco) media supplemented with recombinant murine Chiron 10 μM (Stemgent) ...
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bioRxiv - Systems Biology 2019Quote: ... Metabolites were detected and quantified as area under the curve based on retention time and accurate mass (≤ 8 ppm) using the TraceFinder 3.3 (Thermo Scientific) software ...
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bioRxiv - Immunology 2019Quote: ... were labelled for 20 minutes at room temperature with Cell Trace Violet (CTV, thermofisher) in PBS and cultured at a density of 8×104 cells/well in RPMI 1640 medium (Thermofisher) supplemented with 10% FBS (ThermoFisher) ...
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bioRxiv - Immunology 2019Quote: Cells acquired by BAL were spun down and resuspended in complete RPMI without antibiotics and plated on a Lab-Tek II 8-chamber glass-bottom slide (Nunc). The slides were kept for 2 hours in a 37°C humidified incubator (5% CO2 ...
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bioRxiv - Cell Biology 2019Quote: ... 8×106 HEK293T cells were seeded onto a 15 cm diameter tissue culture dish in 20ml DMEM with Glutamax (Gibco) with 10% heat inactivated FBS (Gibco) ...
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bioRxiv - Genomics 2019Quote: ... The samples were cooled down to RT before loading them on a 3-8% Tris-Acetate Protein Gels (Invitrogen EA0375PK2). Next ...
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bioRxiv - Biophysics 2020Quote: ... The CE was then loaded onto 8 ml prewarmed 7.5% sucrose solution in extraction buffer and microtubules pelleted at 40,000 × g (SS34 rotor, Thermo Fisher Scientific) for 30 minutes at 37 °C ...
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bioRxiv - Cancer Biology 2019Quote: Thymus and femurs from 6-8 week old mice were harvested and subjected to red blood cell lysis (00-4333-57, Invitrogen). Thymocytes and bone marrow cells were counted by Beckman Coulter Z1 counter ...
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bioRxiv - Cancer Biology 2019Quote: ... and primary fibroblasts obtained from lungs of healthy NSG or BALB/c mice 6-8 weeks of age were cultured in Minimum Essential Medium Eagle medium with alpha modification (MEMα) (ThermoFisher) supplemented with 1x MEM Non-essential Amino Acid Solution (Sigma-Aldrich) ...
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bioRxiv - Immunology 2019Quote: ... mixtures were subjected to 8% SDS-PAGE with 6 µl of molecular weight marker (SeeBlue Plus2 Pre-Stained Standard, Invitrogen), after which proteins were transferred to nitrocellulose membranes (Amersham Hybond ECL 0.45 µm Nitrocellulose Membrane ...
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A general role of zinc binding domain revealed by structures of σ28-dependent transcribing complexesbioRxiv - Molecular Biology 2020Quote: ... lysed by ultrasonication and then centrifuged at 80,000 g for 1 h using a T29-8×50 fixed angle rotor (Sorvall LYNX 6000 superspeed centrifuge, Thermo Scientific). For σ28 purification ...
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bioRxiv - Genetics 2019Quote: A set of 4x 40 µl of PCR reaction was mixed with 8 µl 6x DNA load dye (Thermo Scientific) and run on a 2% TBE agarose gel (UltraPure ...
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bioRxiv - Genetics 2020Quote: ... samples were diluted in Laemmli sample buffer and 2 μg of protein was separated on a NuPage 3–8% Tris-Acetate gel (Invitrogen). The gel was then stained with Coomassie Blue and digitalized.
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bioRxiv - Cancer Biology 2020Quote: ... ATM and ATR in the CE samples as follows: the samples were loaded on 4-8% (w/v) Tris-acetate SDS-PAGE gels (ThermoFisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... were lysed in urea buffer (8 M urea, 100 mM NaHPO4, pH 8.5) with HALT protease and phosphatase inhibitor (Pierce/Thermo Scientific) and processed at the Emory Proteomics Core ...
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bioRxiv - Microbiology 2021Quote: ... A 1ml-overlay of 1:1 mixture of 2X Minimal Essential Medium (MEM; Hyclone, SH30008.04) with 8% FBS and 1.5% low melting agarose (ThermoFisher Scientific, R0801) was placed in the wells ...
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bioRxiv - Molecular Biology 2020Quote: ... 25 mg Orange G in 10 mL H2O) was added to each reaction and run on an 8% TBE gel (Invitrogen) (180 V ...
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bioRxiv - Neuroscience 2020Quote: ... siRNAs and Gapmers or shRNAs were introduced to primary hippocampal neurons (5–8 days in vitro (DIV)) using Lipofectamine RNAiMAX or Lipofectamine 2000 (Invitrogen) according to manufacturer’s guidelines ...
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bioRxiv - Bioengineering 2021Quote: ... IL-8 concentrations in medium samples from the basal compartment were measured using the IL-8 Human Uncoated ELISA Kit (88-8086-88, Invitrogen). Assays were performed in Nunc MaxiSorp (tm ...
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bioRxiv - Bioengineering 2020Quote: ... The cells were passaged at a density of 1:2 to 1:8 after reaching ~80% confluency by detaching with 5 mM EDTA in PBS (Invitrogen 15575-038 diluted in sterile 1X PBS ...
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bioRxiv - Biochemistry 2021Quote: ... Purified proteins were dialyzed in Spectra-Por7 semi-permeable dialysis membranes (8 kDa cut-off; Thermo Fisher Scientific, Waltham, MA) for 48 hours against conservation buffer (20 mM Tris pH=8.0 ...
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bioRxiv - Biochemistry 2020Quote: Cells were trypsinized and seeded at 20,000 cells/well in an 8-well chambered coverglass (Nunc™, 12-565-470) with fresh DMEM (+ ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were resolved on NuPage 3-8% w/v Tris-Acetate or 4-12% w/v Bis-Tris gels (Invitrogen) and transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Neuroscience 2021Quote: ... Neurons were then transferred to pre-warmed neuronal media and plated at a density of 2,000 cells per well in an 8-well glass Lab-Tek slide (Thermo Scientific) pre-coated with poly-D-lysine (Corning ...