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Citations for Thermo Fisher :
1901 - 1950 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... The eluate was then sequentially treated with RNase A (Thermo Scientific; 1 h at 37 °C) and Proteinase K (Roche ...
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bioRxiv - Cancer Biology 2024Quote: ... A 2µg/µl protein solution containing sample buffer (Invitrogen Cat# NP0007) plus sample reducing agent (Invitrogen Cat# NP0004 ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA was synthesized using a maximum of 1 μg of total RNA with the High-Capacity cDNA Reverse Transcription Kit (Cat# 4368814, Applied Biosystems). Quantitative RT-PCR was performed using the Taqman Fast Universal PCR Master Mix (Cat# 4352042 ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were washed three times with PBS before incubating with Alexa Fluor anti-mouse IgG secondary (ThermoFisher) for 1 h (1:1000 in 3% BSA) ...
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bioRxiv - Cancer Biology 2024Quote: ... Dnmt3L KO clones (QKO) were generated by transfecting TKO J1 mESC (42) with sgRNA-containing px458 using Lipofectamine 3000 (Invitrogen) and sorting GFP+ cells after 72h ...
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bioRxiv - Cancer Biology 2024Quote: 2.5 × 105 cells were collected and washed with 1 ml of wash buffer (20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM Spermidine (ThermoFisher, #13274)) before being resuspended in 1ml of wash buffer ...
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bioRxiv - Biophysics 2024Quote: ... the full-length human AGO2 was cloned into a pFastBac dual expression vector (Thermo Fisher) containing an N-terminal His-tag followed by a TEV cleavage site and transformed into chemically competent DH10EmBacY cells (Geneva Biotech) ...
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bioRxiv - Biophysics 2024Quote: ... we flushed in streptavidin-coated M270 beads (Dynabeads, Invitrogen) and incubated with samples to form tethers ...
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bioRxiv - Plant Biology 2024Quote: ... and a 50 cm Easy-Spray PepMap C18 analytical column (ES903; ThermoFisher Scientific) warmed to 50 °C ...
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bioRxiv - Plant Biology 2024Quote: ... A total of 1 μg of re-suspended peptide was injected per replicate using and Easy-nLC 1200 system (LC140; Thermo Scientific) mounted with an Acclaim PepMap 100 C18 trap column (Cat# 164750 ...
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bioRxiv - Plant Biology 2024Quote: Peptides were analyzed on a Fusion Lumos Orbitrap mass spectrometer (ThermoFisher Scientific) in data independent acquisition (DIA ...
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bioRxiv - Plant Biology 2024Quote: ... Total proteome peptide fractions were then generated using a Kingfisher Apex (ThermoFisher Scientific) automated sample processing system as outlined by Leutert et al ...
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bioRxiv - Biophysics 2024Quote: ... 1% L-glutamine and 1% penicillin–streptomycin (Life Technologies, Bleiswijk, NLD).
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bioRxiv - Cancer Biology 2024Quote: ... and incubated for 2h at RT with secondary antibody mix of Alexa Fluor 647 anti-rat (Invitrogen, A-21247) and Alexa Fluor 488 anti-mouse (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... CellEvent Caspase-3/7 Green ReadyProbes™ Reagent (Invitrogen, R37111) was used ...
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bioRxiv - Cancer Biology 2024Quote: ... EdU detection was allowed by click-it reaction of Alexa-Fluor 400 as described in the Click-IT EdU Imaging Kits (Invitrogen, C10337). Primary antibodies against FANCD2 1:100 (NovusBio ...
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bioRxiv - Cancer Biology 2024Quote: ... and amplification with Phusion Flash HF PCR master mix (ThermoFisher Scientific, cat. F548S). All purification and size selection steps were performed using AMPure XP Beads ...
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bioRxiv - Cancer Biology 2024Quote: ... coverslips were washed in PBS 1x before incubation with Alexa-Fluor-647 specie-specific secondary antibodies (Thermo Fisher, A32728 and A-21245) and either Hoechst 1:2000 for EdU labelled cells or DAPI 1:1000 for simple IF ...
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bioRxiv - Biophysics 2024Quote: ... and 12.5 μL of 2X Phire Hot Start II PCR Master Mix (Thermo Fisher #F125L). The following thermocycler protocol was used ...
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bioRxiv - Biophysics 2024Quote: ... while the remaining supernatant was incubated with streptavidin agarose (ThermoFisher Scientific, USA) for 60 minutes at 4° C ...
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bioRxiv - Biophysics 2024Quote: ... according to the guidelines provided in the “Bac-to- Bac TOPO Expression System” user manual (Thermo Fisher, Version A, 15 December 2008 A10606). Fresh Sf9 cells were transfected with the recombinant AGO2 Bacmid ...
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bioRxiv - Biophysics 2024Quote: ... adjusted to pH = 7.7) containing 0.5 mg/mL EZ-LinkTM Sulfo-NHS-SS-Biotin (ThermoFisher Scientific, USA) for 20 minutes at 4° C ...
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bioRxiv - Plant Biology 2024Quote: ... seedlings were incubated in half-strength MS liquid culture medium containing 1 mM MitoTracker (Thermo Fisher Scientific, USA) for 30 min ...
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bioRxiv - Cancer Biology 2024Quote: ... 0.04 ug/mL salmon sperm DNA (salDNA; Invitrogen #15632-011), 1 mM DTT ...
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bioRxiv - Biophysics 2024Quote: ... SYPRO Red Protein Gel Stain (5,000X Concentrate in DMSO) was obtained from Invitrogen Molecular Probes.
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bioRxiv - Biophysics 2024Quote: ... Cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM - Life Technologies, Paisley, GBR) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Biophysics 2024Quote: ... and subsequently following SYBR Gold staining (Invitrogen), using an ImageQuant LAS-4000 imager (GE healthcare) ...
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bioRxiv - Biophysics 2024Quote: ... which is a mix of ECM components derived from murine Engelbreth-Holm-Swarm (EHS) tumors (Thermo Fisher Scientific Inc ...
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bioRxiv - Biophysics 2024Quote: ... The gels were then stained using SYBR Gold according to the manufacturer’s protocol (Invitrogen) and imaged with a GE Healthcare ImageQuant LAS 4000.
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bioRxiv - Biophysics 2024Quote: ... Oocytes were then homogenized in HEN solution in the presence of protease inhibitors (ThermoFisher Scientific, USA) and 1% Triton X-100 ...
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bioRxiv - Biophysics 2024Quote: ... 1.875 μL of bovine serum albumin (20 mg/mL, Thermo Fisher #B14), and 37.5 μL of 2X Phire Hot Start II PCR Master Mix (Thermo Fisher #F125L) ...
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bioRxiv - Biophysics 2024Quote: ... Samples were loaded on a DNAPac PA200 column (22×250 mm; Thermo Fisher, Thermo Fisher Ultimate 3000 system) at 75 °C under low salt conditions in buffer A (20 mm NaOAc pH 6.5 ...
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bioRxiv - Biophysics 2024Quote: ... the 2A3 modified RNA and corresponding no modification control were reverse transcribed by SuperScript II Reverse Transcriptase (Thermo Fisher #18064022), using primers listed in Supplemental Table S9 ...
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bioRxiv - Biophysics 2024Quote: ... and secondary antibody (Anti Rabbit IgG, ThermoFisher Scientific ...
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bioRxiv - Biophysics 2024Quote: ... or pcDNA 3.1+ (Thermofisher Scientific, USA) vectors for expression in Xenopus oocytes and HeLa cells ...
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bioRxiv - Biophysics 2024Quote: ... Protein concentration was determined using a Bradford assay (Thermo Fisher PierceTM Detergent Compatible Bradford Assay Kit ...
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bioRxiv - Biophysics 2024Quote: ... The Signal Enhancer HIKARI (Nacalai Tesque, INC, Japan) was used for incubating the primary antibody (anti-Cx30, ThermoFisher Scientific ...
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bioRxiv - Biophysics 2024Quote: ... the RPMI medium was exchanged with a D2O medium obtained by dissolving RPMI-1640 powder (Gibco) in 100% D2O ...
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bioRxiv - Biophysics 2024Quote: ... and 37.5 μL of 2X Phire Hot Start II PCR Master Mix (Thermo Fisher #F125L). The oil phase mixture was chilled on ice for 30 minutes and transferred to a cold glass vial ...
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bioRxiv - Biophysics 2024Quote: ... and secondary antibody (Anti Rabbit IgG, ThermoFisher Scientific, Cat. # 32460 or Anti Mouse IgG, ThermoFisher Scientific, Cat. # 31430, 1:10,000). SuperSignal® West Femto (ThermoFisher Scientific ...
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bioRxiv - Biophysics 2024Quote: ... All fractions from both the HisTrap-Ni2+ and SEC columns were analysed using SDS-PAGE (Invitrogen, NuPAGE 4 to 12%, Bis-Tris, 1.0 mm) to assess purity (Figure S2B) ...
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bioRxiv - Biophysics 2024Quote: ... and plasmids were purified using a PureLink Plasmid Miniprep Kit (Invitrogen, #K210011) or a Qiagen Plasmid Midi Kit (Qiagen ...
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bioRxiv - Biophysics 2024Quote: ... 50 µg/mL Streptomycin (Life Technologies), and 1% Glu-taMAX (Life Technologies ...
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bioRxiv - Biophysics 2024Quote: ... Image-iT FX Signal Enhancer (catalog no. I36933) was obtained from Invitrogen. SNAP-Surface Alexa Fluor 647 (catalog no ...
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bioRxiv - Biophysics 2024Quote: ... supplemented with 10% fetal bovine serum (FBS, Gibco). The cells were tested for mycoplasma contamination (Mycoplasmacheck ...
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bioRxiv - Biophysics 2024Quote: ... the quality of the deflection was tested by measuring intensity profiles along z on 100 nm fluorescent beads (FluoSpheres, 0.1 µm, Tetraspeck; Thermo Fisher Scientific) or single molecules on DNA origami (ATTO647N ...
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bioRxiv - Biophysics 2024Quote: ... Fetal Bovine Serum (FBS) (catalog no. 10500064) and trypsin-EDTA 0.5% (catalog no. 15400054) were obtained from Gibco. L-glutamine (catalog no ...
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bioRxiv - Biophysics 2024Quote: ... and then incubated with 50-100 μl 2.3 μM (0.1mg/ml) GST-WCA and various concentrations of Neutravidin (ThermoFisher, Cat. #31000) (8.3 μM (0.5mg/ml ...
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bioRxiv - Biophysics 2024Quote: ... 50 units/mL Penicillin (Life Technologies), 50 µg/mL Streptomycin (Life Technologies) ...
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bioRxiv - Biophysics 2024Quote: ... to which 2.5 μL of ddH2O and 2.5 μL of reverse transcription (RT) mix (1 μL 5X First strand buffer (Thermo Fisher), 0.01 M DTT ...