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Citations for Thermo Fisher :
2101 - 2150 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... Peptides were TMT labelled using the TMT 10-plex isobaric label reagents (Thermofisher) and fractionated on a Waters XBridge BEH C18 (3.5 μm ...
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bioRxiv - Molecular Biology 2024Quote: ... and Phalloidin 647 Plus (Invitrogen, A30107, 1:400). Plates were imaged at 10x (MRF00101 ...
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bioRxiv - Molecular Biology 2024Quote: ... Immunoprecipitated complexes were collected using Protein G Dynabeads (Invitrogen) and washed with 1000 μL of respectively ...
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bioRxiv - Molecular Biology 2024Quote: ... Fractionated samples and bead-enriched samples were individually loaded onto a C18 trap column (3 μm, 75μm × 2 cm, Thermo Fisher Scientific) connected in-line to a C18 analytical column (2 μm ...
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bioRxiv - Molecular Biology 2024Quote: ... and Phalloidin 647 Plus (Invitrogen, A30107, 1:400). Plates were imaged at 10x (MRF00101 ...
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bioRxiv - Molecular Biology 2024Quote: ... Peptides were ionized using a nanospray ion source into a Q-Exactive HF mass spectrometer (Thermo Fisher Scientific). MS/MS spectra were searched against the Uniprot human complete proteome FASTA database downloaded on 2018_10_26 ...
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bioRxiv - Molecular Biology 2024Quote: ... Hoechst 33342 (Invitrogen, H3570, 1:1000, 10µg/mL), and Phalloidin 647 Plus (Invitrogen ...
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bioRxiv - Molecular Biology 2024Quote: ... FLAG M2 or HA-tag antibody at 4 °C overnight before precipitated with magnetic Protein G beads (Thermofisher) for 2 hours at 4 °C followed by 3 washes with RIPA buffer.
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bioRxiv - Cancer Biology 2024Quote: ... V5-tag (46-0705, Invitrogen)(1.12 ug antibody/500 ug protein IP ...
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bioRxiv - Cancer Biology 2024Quote: Transfections of siRNAs were performed using Lipofectamine 3000 (L3000015, Thermo Fisher Scientific). Cells were reverse transfected with final siRNA concentration of 12.5 nM siRNA ...
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bioRxiv - Cancer Biology 2024Quote: Cells were washed with PBS and harvested in TRIzolTM reagent containing phenol (15596018, Life Technologies). For total RNA extraction ...
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bioRxiv - Cancer Biology 2024Quote: crRNA:tracrRNA duplexes were transfected into RPE Cas9 cells by reverse transfection using Lipofectamine 3000 transfection reagent (L3000015, Thermo Fisher Scientific) with a final crRNA:tracrRNA concentration of 25 nM (12.5 nM upstream crRNA:tracrRNA ...
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bioRxiv - Cancer Biology 2024Quote: ... and the upper aqueous phase was used for precipitating the RNA by adding 500 µL isopropanol – and optionally 2 µL GlycoBlue Coprecipitant (AM9516, Thermo Fisher Scientific) – and centrifuging ...
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bioRxiv - Cancer Biology 2024Quote: ... 2% SDS) and 2 µL 20 mg/mL proteinase K (25530049, Thermo Fisher Scientific). Samples were incubated overnight at 65 °C and 800 rpm ...
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bioRxiv - Cancer Biology 2024Quote: ... and resuspended in nuclease-free H2O (AM9939, Life Technologies). RNA concentration was measured by spectrophotometry.
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bioRxiv - Cancer Biology 2024Quote: For immunoblotting cells were harvested in RIPA lysis buffer supplemented with a protease and phosphatase inhibitor cocktails (Thermo Fisher). Lysis was allowed to proceed for 30-60 minutes on ice ...
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bioRxiv - Cancer Biology 2024Quote: ... and chromatin fractions was carried out using a Subcellular Protein Fractionation Kit (Thermo Fisher #78840).
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bioRxiv - Cancer Biology 2024Quote: ... cells were incubated in PBS with 1.5 mM dithiobis (succinimidyl propionate) (DSP, Thermo Fisher #22585) for 20 min ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell nuclei were then extracted and resuspended in 1/2 dilution IP buffer (Thermo Fisher #87788) and sonicated for 2 min (5s on/5s off ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein concentrations were determined by Bicinchoninic acid (BCA) assay (Thermo Fisher). Lysates were prepared with 4X Laemmli buffer (BioRad ...
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bioRxiv - Cancer Biology 2024Quote: ... rat anti-mouse MHCII (Invitrogen, 14-5321-82, IHC-F), rabbit anti-human SFTPC (Millipore ...
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bioRxiv - Cancer Biology 2024Quote: ... rat anti-Ki67 (Invitrogen, 14-5698-82, AR=CB), rat anti-mouse MHCII (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... or Wheat Germ Agglutinin (WGA) AlexaFluor 647 (Invitrogen, W11261) diluted in blocking solution for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: The microbeads were treated with 1mg/ml of collagenase (Thermo Fisher, Waltham, Massachusetts, USA) for 7 min at 37°C in a humidified incubator until beads were dissolved ...
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bioRxiv - Cancer Biology 2024Quote: ... mice were sacrificed and livers were collected in 4% paraformaldehyde (Thermo Fisher Scientific #AAJ19943K2) and shaken overnight at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... 500 µl of Novec 7500 containing 20 % 1H,1H,2H,2H-perfluorooctanol (PFO; ThermoFisher Scientific GmBH, Germany) was added to the suspension and mixed by pipetting resulting in bead clustering ...
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bioRxiv - Cancer Biology 2024Quote: Mitochondrial mass was analyzed by flow cytometry using the MitoTracker™ Green FM Dye (Invitrogen). 0.5 x 106 cells were transferred to the FACS tubes ...
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bioRxiv - Cancer Biology 2024Quote: A LIVE/DEAD™ Viability/Cytotoxicity Kit for mammalian cells (Thermo Fisher, USA) was used to stain the live and dead cells ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell Proliferation Dye eFluor™450 (Invitrogen) was used to label HS-5 cells before setting up a co-culture with K562 cells ...
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bioRxiv - Cancer Biology 2024Quote: ... Supernatant was collected and the concentration of protein lysate was quantified by BCA assay (Thermo Fisher Scientific, 23225). Cell lysates were mixed with Laemmli sample buffer (BIO-RAD ...
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bioRxiv - Cancer Biology 2024Quote: ... 1× B27 (Invitrogen, 17504-044), 1 mmol/L N-acetylcysteine (Sigma- Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... Images were taken using an EVOS® FL Auto Imaging System (ThermoFisher Scientific) at 20x magnification ...
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bioRxiv - Cancer Biology 2024Quote: ... and cells were cultured for 2 days in IMDM (Thermo Fisher Scientific, #12440053) with 15% fetal bovine serum (Gemini Bio ...
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bioRxiv - Cancer Biology 2024Quote: ... HCT116 parental and transfected cells were cultured in McCoy’s (5A)-modified (Gibco; Life technologies) supplemented with 10% (v/v ...
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bioRxiv - Cancer Biology 2024Quote: ... parental cells were transfected with All-in-One nickase plasmids via electroporation using the amaxa-nucleofector® II according to the manufacturer’s instructions (Thermofisher). 5-10 μg of the All-in-One plasmid were transfected into 106 cells ...
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bioRxiv - Cancer Biology 2024Quote: ... and 1% (v/v) sodium pyruvate 100X (Gibco; Life technologies). HCT116 parental and transfected cells were cultured in McCoy’s (5A)-modified (Gibco ...
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bioRxiv - Cancer Biology 2024Quote: ... HCT116 parental and transfected cells were cultured in McCoy’s (5A)-modified (Gibco; Life technologies) supplemented with 10% (v/v ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins separated by NuPAGE™ 4-12% Bis-Tris precast protein gels (Thermo Fisher Scientific), and then transferred to nitrocellulose membrane (GE Healthcare) ...
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bioRxiv - Cancer Biology 2024Quote: ... and 1% (v/v) sodium pyruvate 100X (Gibco; Life technologies). HCT116 parental and transfected cells were cultured in McCoy’s (5A)-modified (Gibco ...
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with 10% fetal bovine serum (Life Technologies), 2 mM glutamine (Sigma) ...
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bioRxiv - Molecular Biology 2024Quote: ... AssayAssure® (Thermo Scientific, SKU 14401) was added to half of the samples prior to simulated storage.
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bioRxiv - Molecular Biology 2024Quote: ... 2008) N-terminally tagged with the V5 epitope was synthesized as a DNA string (GeneArt Strings DNA Fragments, Thermo Fisher Scientific, Schwerte, Germany) and cloned into pcDNA3.1 to obtain pcDNA3.1-V5-RHEB-Q64L ...
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bioRxiv - Molecular Biology 2024Quote: ... Ultimate 3000 Rapid Separation UHPLC system equipped with a PepSep C18 analytical column (15 cm, ID 75 μm, 1.9 μm Reprosil, 120 Å) coupled to a Q Exactive HF mass spectrometer (Thermo Scientific). An aliquot of 2 μL (tissue piece ...
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bioRxiv - Molecular Biology 2024Quote: ... Untargeted metabolomics data were collected using ultra-high-performance liquid chromatography system (Vanquish, Thermo Fisher Scientific, Waltham, MA, USA) with a Kinetex 1.7 um 100 A pore size C18 reversed phase UHPLC column 50 × 2.1 mm (Phenomenex ...
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bioRxiv - Molecular Biology 2024Quote: ... anti-V5 (R960-25, Thermo Fisher Scientific); anti-HA (clone 3F10 ...
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bioRxiv - Cancer Biology 2024Quote: ... and incubated in primary antibodies diluted in 5% (w/v) BSA in TBST with 0.01% (w/v) sodium azide (Fisher Scientific, S227I-25), rocking at 4°C overnight ...
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bioRxiv - Cancer Biology 2024Quote: ... bromophenol blue (Fisher Scientific, BP115-25), 5% (v/v ...
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bioRxiv - Cancer Biology 2024Quote: ... Sample concentrations were normalized using 2X SDS sample buffer (62.5 mM Tris pH 6.8, 2% SDS, 10% glycerol (Fisher Scientific G33-500), bromophenol blue (Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... 302 mM dithiothreitol (DTT) (Fisher Scientific, BP172-25) and RIPA lysis buffer) ...
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bioRxiv - Cancer Biology 2024Quote: ... 20 mM sodium fluoride (NaF; Fisher Scientific, S25547), 0.5% (v/v ...