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Citations for Thermo Fisher :
1951 - 2000 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... trophozoites were fixed with DSP solution (Thermo Fisher, Massachusetts, USA). The cells ...
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bioRxiv - Cell Biology 2024Quote: ... The BN-Page was performed in XCell™ SureLock™ Mini-Cell (Thermo Fisher Scientific, Massachusetts, USA) followed by electro transferring onto nitrocellulose membranes mentioned above.
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bioRxiv - Cell Biology 2024Quote: ... and cDNA was synthesized with SuperScript III (Invitrogen) using random primers p(dN)6 (Roche) ...
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bioRxiv - Cell Biology 2024Quote: ... The permeabilized samples were added with NativePage buffer (Thermo Fisher Scientific, Massachusetts, USA) and Coomassie G-250 dye ...
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bioRxiv - Cell Biology 2024Quote: Blue Native-PAGE (BN-Page) was conducted following the protocol from NativePAGE™ Novex® Bis-Tris Gel System kit (Thermo Fisher Scientific, Massachusetts, USA). Samples separated by membrane fractionation were utilized in BN-Page ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA was synthesized using SuperScriptIII reverse transcriptase (Invitrogen) with gene-specific primers (Table S5 ...
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bioRxiv - Molecular Biology 2024Quote: ... and 10U TURBO DNase (Invitrogen) for 5 min at 37°C with rotation and centrifugation (13000 rpm ...
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bioRxiv - Molecular Biology 2024Quote: ... The DNA concentration was determined using using a Qubit instrument (Invitrogen, USA) and the size distribution of the fragments was analyzed on an Agilent Bioanalyzer (DNA 100 assay) ...
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bioRxiv - Molecular Biology 2024Quote: ... before being incubated for 20 min at 4°C with anti-V5-FITC or anti-V5-PE (ThermoFisher Scientific) and anti-CD8-APC (Miltenyi Biotec) ...
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bioRxiv - Molecular Biology 2024Quote: ... and pcDNA-FRT-TO-CHD4-GFP (kind gift from Gernot Längst) was performed using Lipofectamine 2000 (Invitrogen) and Opti-MEM (Gibco ...
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bioRxiv - Molecular Biology 2024Quote: Primary dermal fibroblasts were from a forearm skin punch biopsy from the proband and cultured in Dulbecco’s Minimal Essential Media (DMEM) containing 4.5 g/L glucose supplemented with 10% heat inactivated fetal bovine serum and 1% antibiotic-antimycotic (Life Technologies, Carlsbad, CA). Control cells obtained from Coriell Institute (Camden ...
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bioRxiv - Molecular Biology 2024Quote: ... cloned into pCR4-TOPO vector (ThermoFisher), and sequenced using vector-specific primers ...
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bioRxiv - Molecular Biology 2024Quote: ... digested peptides were acidified with 0.8% TFA (final) and analyzed by LC– MS/MS on an EASY-nLC 1000 (Thermo Scientific) with a two-column setup ...
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bioRxiv - Molecular Biology 2024Quote: ... 5 ug of empty vector or FLAG-POLR3B was transfected per well using lipofectamine 2000 (Invitrogen). After 24h ...
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bioRxiv - Molecular Biology 2024Quote: ... Approximately 5 ug total RNA was separated in 10% polyacrylamide-TBE-UREA gel (ThermoFisher) using formamide loading buffer (Thermofisher ...
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bioRxiv - Cell Biology 2024Quote: ... anti-rabbit Alexa Flour-647 conjugated antibody (Cat# A-31573, Invitrogen), and anti-mouse ...
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bioRxiv - Cell Biology 2024Quote: ... and HRP-conjugated anti-mouse IgG (Cat. No. 31430, Invitrogen). Finally ...
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bioRxiv - Cell Biology 2024Quote: ... cells were incubated with Wheat Germ Agglutinin (WGA) conjugated with Alexa Flour (AF) 647 (Cat# W32466, Invitrogen) to stain cell glycocalyx and phalloidin conjugated with Alexa Flour (AF ...
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bioRxiv - Cell Biology 2024Quote: ... cell lysates were incubated overnight with either the anti-ITGβ1 antibody (Cat# PA578028, Invitrogen) or the control IgG antibody (Cat# CR1 ...
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bioRxiv - Cell Biology 2024Quote: ... cells were incubated with one of the following secondary antibodies at RT for 2 hours: anti-rabbit Alexa Flour-488 conjugated antibody (Cat# A-11008, Invitrogen), anti-rabbit Alexa Flour-555 conjugated antibody (Cat# A-21428) ...
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bioRxiv - Cell Biology 2024Quote: ... to stain cell glycocalyx and phalloidin conjugated with Alexa Flour (AF) 555 (Cat# A34055, Invitrogen) to stain F-actin stress fibre ...
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bioRxiv - Cell Biology 2024Quote: ... When using the β-galactosidase stain C12FDG (5-Dodecanoylaminofluorescein Di-β-D-Galactopyranoside, ThermoFisher), cells were incubated with C12FDG dye for 2 hoursLat 37°C and washed twice before staining with surface marker antibodies ...
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bioRxiv - Cell Biology 2024Quote: ... BM cells were fixed and permeabilized using the FIX & PERM™ Cell Permeabilization Kit (ThermoFisher) per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Red cells were lysed using 1X red blood cell lysis buffer (ThermoFisher). Cells were then stained with a combination of fluorochrome-conjugated antibodies from eBioscience ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were immediately counted on a Countess II automated cell counter (ThermoFisher) and 12,000 cells were loaded on to one lane of a 10X Chromium microfluidic chip (10X Genomics).
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bioRxiv - Cell Biology 2024Quote: ... qRT-PCR was performed using Power SYBR™ Green PCR Master Mix (Applied Biosystems; cat. #4368577). PCR conditions were pre-amplification (50L°C/120Lseconds ...
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bioRxiv - Cell Biology 2024Quote: Four paired samples of conditioned medium (serum-free basal medium [DMEM/F12] supplemented with 1% ITS [Insulin-Transferrin-Selenium; #41400045 Life Technologies]) were collected over a 24-h period from glutamine-depleted and glutamine-replete HeLa cells ...
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bioRxiv - Cell Biology 2024Quote: ● Magnesium chloride (MgCl2) (Fisher Scientific).
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bioRxiv - Cell Biology 2024Quote: ● Glycerol (Fisher Scientific).
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bioRxiv - Cell Biology 2024Quote: ● BY4741 (MATa, his3Δ1, leu2Δ0, met15Δ0, ura3Δ0) (Invitrogen).
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bioRxiv - Cell Biology 2024Quote: ● Tris Base (Fisher Scientific).
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bioRxiv - Cell Biology 2024Quote: ... fluorescent blue/green/orange/dark red (Invitrogen).
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bioRxiv - Cell Biology 2024Quote: ● AttofluorTM chambers (Invitrogen).
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bioRxiv - Molecular Biology 2024Quote: ... HEK293T and U2OS cells were transfected using Lipofectamine 3000 (Thermo Fisher Scientific) following manufacturer’s instructions.
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bioRxiv - Molecular Biology 2024Quote: Reverse siRNA transfections were done using 5 nM Silencer Select pre-designed siRNAs (Thermo Fisher Scientific) with Lipofectamine RNAiMAX (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were heated to 80°C for 5 min and electrophoresed on 4-12% NuPAGE Bis-Tris gels (ThermoFisher). Wet transfer was to a nitrocellulose membrane (0.45 uM pore ...
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bioRxiv - Molecular Biology 2024Quote: ... anti-γ-actin (Thermo Scientific, PA1-16890) at 1:5000 ...
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bioRxiv - Molecular Biology 2024Quote: ... and Streptavidin M280 dynabeads (Invitrogen) used for pulldown ...
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bioRxiv - Molecular Biology 2024Quote: ... connected to an Orbitrap Fusion (Thermo Scientific) at 150 uL/min flow rate ...
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bioRxiv - Molecular Biology 2024Quote: ... using formamide loading buffer (Thermofisher, AM8547) and transferred to a positively charged nylon membrane (GeneScreen plus ...
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bioRxiv - Molecular Biology 2024Quote: ... addition of 2 ug sheared salmon sperm DNA (ThermoFisher, AM9680) in the loading buffer (Thermofisher ...
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bioRxiv - Molecular Biology 2024Quote: ... using the iBlot2 dry blotting system (ThermoFisher). Similar to what has been found for other low abundance small RNAs ...
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bioRxiv - Molecular Biology 2024Quote: ... Reverse transcription was carried out with 1 μg of total RNA using random hexamer primers and the SuperScript IV reverse transcriptase kit (Thermo Fisher Scientific). Quantitative PCR was performed using Kapa SYBR Green on a Lightcycler 480 (Roche) ...
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bioRxiv - Molecular Biology 2024Quote: ... Relative quantitation of metabolites was performed with XCalibur QuanBrowser 2.2 (Thermo Fisher Scientific) using a 5 ppm mass tolerance and referencing a retention time for uracil from an in-house library of chemical standards ...
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bioRxiv - Molecular Biology 2024Quote: ... and 100 µg/mL RNAse (Invitrogen) for 1 hour at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA was synthesized using POLR3B exon 18-specific primer 5’-TGTTGTGTTCGTACAGTGCAATGT and the Superscript III First-strand synthesis system (ThermoFisher). With the first strand cDNA as a template ...
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bioRxiv - Molecular Biology 2024Quote: ... Peptides were applied on an Acclaim PepMap 100 C18 trap column (75 µm ID × 2 cm, 3 µm; Thermo Scientific) in 0.1% formic acid and 2% acetonitrile in H2O at a constant pressure of 80 MPa and separated by a linear gradient of 2%–6% buffer B in buffer A for 3 min ...
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bioRxiv - Molecular Biology 2024Quote: ... equipped with an Ion Max source and a HESI II probe (Thermo Fisher Scientific). Samples were separated by chromatography by injecting 2 μl of sample on a SeQuant ZIC-pHILIC Polymeric column (2.1 × 150 mm 5 μM ...
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bioRxiv - Molecular Biology 2024Quote: ... slides were stained with SYBR® Gold Nucleic Acid Gel Stain (ThermoFisher) and visualized at 488 nm and 10x magnification using a Nikon 90i microscope ...
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bioRxiv - Molecular Biology 2024Quote: ... Wet transfer was to a nitrocellulose membrane (0.45 uM pore, ThermoFisher) in a Mini-Cell (ThermoFisher ...