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Citations for Thermo Fisher :
1851 - 1900 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... and Alexa 488-labeled anti-IgG antibody (Invitrogen) was added at a 1:1,000 dilution for 1 h at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... were propagated in DMEM Advanced (Gibco No. 12491015) supplemented with 5% fetal bovine serum (FBS ...
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bioRxiv - Genomics 2024Quote: ... on a QuantStudio™ 5 Real-Time PCR System (Applied Biosystems™). Expression of all genes was normalized to act5C or vglut and relative levels were calculated versus control using the ΔCt method(24) ...
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bioRxiv - Microbiology 2024Quote: ... 5 µl of each sample was loaded on a C18 precolumn (300 µm inner diameter × 5 mm, Thermo Fisher Scientific) in a solvent made of 2% acetonitrile and 0.05% trifluoroacetic acid ...
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bioRxiv - Microbiology 2024Quote: ... 20 µL of each sample was loaded on NuPAGE 4 to 12% Bris-Tris gels (Invitrogen), and electrophoresis was performed according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... using an xCell II blot module system (Invitrogen). To detect PBP2a ...
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bioRxiv - Microbiology 2024Quote: ... proteins were blotted onto a polyvinylidene difluoride (PVDF) membrane (Invitrogen) using an xCell II blot module system (Invitrogen) ...
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bioRxiv - Microbiology 2024Quote: ... Detection of the specific protein signal was achieved by using WesternBreeze Chemiluminescent (anti-rabbit or anti-mouse) kit (Invitrogen) or by using Immobilon Forte Western HRP substrate (Merck)
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bioRxiv - Microbiology 2024Quote: The plasmids for the recombinant SARS-CoV-2 variants were transiently transfected into Expi293F cells (Thermo Fisher Scientific) with the ExpiFectamine 293 Transfection Kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... HyperSil Gold column as a trap column (Thermo Fisher Scientific), Accelaim PepMap300 C18 column as an analytical column (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... and Orbitrap Eclipse for mass measurement of digested peptides (Thermo Fisher Scientific). The target protein for hydrogen/deuterium exchange epitope mapping was a chimeric protein of the spike protein of SARS-CoV-2 expressed in HEK293 cells ...
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bioRxiv - Microbiology 2024Quote: ... Expi293F cells (Thermo Fisher Scientific) were maintained in Expi293 expression medium (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... were maintained in Expi293 expression medium (Thermo Fisher Scientific) at 37 °C under 8% CO2.
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bioRxiv - Microbiology 2024Quote: ... were maintained in ExpiCHO expression medium (Thermo Fisher Scientific) at 37 °C under 8% CO2 ...
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bioRxiv - Immunology 2024Quote: RNA was extracted from a homogenized stomach biopsy in TRIzol (ThermoFisher Scientific) and precipitated from the aqueous phase using an equal volume of 70% ethanol ...
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bioRxiv - Immunology 2024Quote: ... Beads were washed twice with FACS buffer and stained with a mixture of anti-mouse IgG Alexa Fluor™ 647 (ThermoFisher, Cat. #A-21235, 1:3000) and anti-human IgG Alexa Fluor™ 647 (ThermoFisher ...
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bioRxiv - Immunology 2024Quote: ... and anti-human IgG Alexa Fluor™ 647 (ThermoFisher, Cat. #A-21445, 1:3000) for 10 min at room temperature in dark ...
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bioRxiv - Immunology 2024Quote: ... for 2 h at 4°C and processed through Zeba spin desalting columns (ThermoFisher) to remove excess unbound biotin.
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bioRxiv - Biophysics 2024Quote: ... were cultured in PLAT media (Gibco DMEM liquid medium (-sodium bicarbonate ...
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bioRxiv - Biophysics 2024Quote: ... Cell culture was carried out in polystyrene T75 culture flasks (Thermo Scientific). Cells were routinely tested for mycoplasma ...
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bioRxiv - Biophysics 2024Quote: ... and manually blotted for ∼3 seconds with 55 mm Whatman filter paper (ThermoFisher) to obtain a thin film ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were stained for anti-Ki67 (Thermo Fisher Scientific; cat# 56-5698-82), anti-TNFα (BioLegend ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein concentration was determined using Pierce 660 nM Protein Assay Reagent (Thermo Fisher Scientific, Cat# 22660), and 20–30 μg of total protein was loaded in each lane ...
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bioRxiv - Cancer Biology 2024Quote: ... prostates were digested with collagenase Type II (Gibco) for 1 hour at 37°C followed by TryLE (Gibco ...
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bioRxiv - Cancer Biology 2024Quote: ... the sections were chromogenically stained with DAB and counterstained with 50% Gill’s Hematoxylin I (Fisher Scientific, Rochester, NY).
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bioRxiv - Cancer Biology 2024Quote: ... 50 mM GlutaMAX (Life Technologies), penicillin (100 U/ml) ...
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bioRxiv - Cancer Biology 2024Quote: ... KE7- cells were transduced with each of the pCL20c- ETV7-Actp-blasticidin lentiviral vectors and selected for 1 week with 1 µg/ml blasticidin (Fisher Scientific). Resistant cells were seeded in methylcellulose [1.5% methylcellulose (Sigma ...
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bioRxiv - Cancer Biology 2024Quote: ... Quantitative RT-PCR was performed using the Taqman Fast Universal PCR Master Mix (Cat# 4352042, Applied Biosystems) and a StepOnePlus real-time PCR system (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2024Quote: ... were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Invitrogen) with 10% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2024Quote: Whole cell lysates were made in SDS Lysis Buffer (ThermoFisher) and resolved on 3-8% or 4-12% gradient SDS-PAGE gels (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... in MOPS buffer (Thermo Fisher Scientific, Cat# NP0001). Following electrophoresis ...
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bioRxiv - Cancer Biology 2024Quote: ... 1× GlutaMax (100x Gibco #35050-061) and 0.1 mM 2-mercaptoethanol (Gibco)).
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bioRxiv - Cancer Biology 2024Quote: ... and 0.1 mM 2-mercaptoethanol (Gibco)).
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bioRxiv - Cancer Biology 2024Quote: ... 1× nonessential amino acids (100x, Gibco #11140), 1× GlutaMax (100x Gibco #35050-061 ...
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bioRxiv - Cancer Biology 2024Quote: ... in the extracellular domain of the CaSR at a concentration of 5 μg/ml and counterstained with SYTO13 fluorescent nucleic acid dye (ThermoFisher, S7575) as previously described 33 ...
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bioRxiv - Cancer Biology 2024Quote: ... and resolved on 3-8% or 4-12% gradient SDS-PAGE gels (Invitrogen, NuPAGE). After transfer to a polyvinylidene difluoride (PVDF ...
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bioRxiv - Synthetic Biology 2024Quote: ... and 1% penicillin-streptomycin (GIBCO). Cells were grown with 5% CO2 at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... sections were incubated in donkey anti-goat Alexa-Fluor 594 (1:500, Thermofisher) in PB for 1.5 hours at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: To prepare [antibody: bead] complexes 75ul of Protein A Dynabeads (Invitrogen, #10002D) per ChIP sample were washed three times with PBS + 0.01% Tween20 (collecting on a magnet at each step ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were then counterstained with 4′,6-diamidino-2-phenylindole (DAPI) (ThermoFisher) solution in PBS for 15 min and washed three times with PBS ...
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bioRxiv - Cancer Biology 2024Quote: ... 4% to 12% Bis-Tris acrylamide gels (Thermo Fisher Scientific, Cat# NP0322) in MOPS buffer (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... Relative mRNA expression was determined using the standard curve method with the StepOne software v2.3 (Applied Biosystems).
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bioRxiv - Cancer Biology 2024Quote: ... The remaining layer of floating adipocytes was lysed for protein analysis using RIPA buffer supplemented with a cocktail of protease inhibitors (Thermo Scientific Cat#78429), 50 mM NaF ...
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bioRxiv - Plant Biology 2024Quote: ... the coding sequence of both genes was amplified from cDNA with attB attachment sites for subsequent cloning into a pDONR223 (Invitrogen, USA) and recombination with pK7FWG2 (VIB Ghent ...
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bioRxiv - Plant Biology 2024Quote: ... Luminescence was measured using a plate reader (Luminoskan Ascent 2.1, Thermo Fisher Scientific, USA). Background luminescence was recorded by scanning each well 12 times at 10 s intervals ...
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bioRxiv - Plant Biology 2024Quote: ... the coding sequence of CEP4 (AT2G35612) was synthesized (Twist Bioscience, USA) with attB attachment sites for subsequent gateway cloning into pDONRZeo (Invitrogen, USA) and recombination with pB7WG2 (VIB ...
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bioRxiv - Plant Biology 2024Quote: ... For pRLK7 the 1957 bp promoter sequence upstream from the start codon was amplified with primers containing attB attachment sites for subsequent gateway cloning into pDONRZeo (Invitrogen, USA) and recombination with promotor::Venus (YFP)-H2B destination vector (78) ...
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bioRxiv - Cancer Biology 2024Quote: ... hypothalamus and AP lysates was isolated using PureLink RNA columns (Cat# 12183025, Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... These were co-transfected with piggybac transpose vector using Lipofectamine 3000 (Invitrogen) to the QKO mESC cell line ...
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bioRxiv - Cancer Biology 2024Quote: ... and a StepOnePlus real-time PCR system (Applied Biosystems). The following TaqMan primer sets were used ...