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Citations for Thermo Fisher :
1701 - 1750 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2024Quote: ... 5 % CO2 and 95 % rH in Dulbecco’s Modified Eagle’s Medium (Gibco), supplemented with 20 % (v/v ...
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bioRxiv - Systems Biology 2024Quote: ... 490 μM L-glutamine (Gibco), 15 % (v/v ...
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bioRxiv - Systems Biology 2024Quote: ... 490 μM L-glutamine (Gibco), 5.3 mM CaCl2 ...
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bioRxiv - Systems Biology 2024Quote: ... and 1% (v/v) Penicillin-Streptomycin (Gibco). 2 U/mL erythropoietin (Erypo Epoetin alfa FS ...
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bioRxiv - Systems Biology 2024Quote: ... supplemented with 20 % (v/v) fetal calf serum (Gibco) and 1 % (v/v ...
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bioRxiv - Systems Biology 2024Quote: ... supplemented with 20 % (v/v) fetal calf serum (Gibco) and 1% (v/v ...
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bioRxiv - Systems Biology 2024Quote: ... and 1 % (v/v) Penicillin-Streptomycin (Gibco). For the experiment ...
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bioRxiv - Systems Biology 2024Quote: ... Cells were cultured in a water-saturated incubator at 37 °C with 5% CO2 in RPMI-1640 containing L-glutamine (Gibco, catalog # 11875093) supplemented with 10% fetal bovine serum (ATCC ...
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bioRxiv - Systems Biology 2024Quote: ... Peptides were eluted with 0.2% trifluoroacetic acid for 10 minutes and subjected to an additional phosphopeptide enrichment step using High-Select™ Fe-NTA spin columns (Thermo Fisher Scientific, catalog # A32992) to reduce the abundance of non-phosphorylated peptides which may have bound nonspecifically to the IP beads ...
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bioRxiv - Systems Biology 2024Quote: ... Samples were resuspended in 50 mM HEPES and labeled with TMTpro isobaric mass tags (Thermo Fisher Scientific, catalog # A52045) at a TMT:peptide ratio of 4:1 (w/w ...
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bioRxiv - Systems Biology 2024Quote: Raw files containing phosphopeptide mass spectra were processed using Proteome Discoverer version 3.0 (Thermo Fisher Scientific) and searched using Mascot version 2.4 (Matrix Science) ...
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bioRxiv - Systems Biology 2024Quote: ... Supernatants were evaporated overnight (SC110A SpeedVac Plus, ThermoFisher, Waltham, MA, USA), resuspended in 200 μL of methanol:NH4OH 10 mM (7:3 ...
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bioRxiv - Systems Biology 2024Quote: ... and carotenes were extracted with glass beads and 500 μL of acetone in three 20 s rounds of agitation at 0.05 m/s with a FastPrep FP120 cell disruptor (ThermoFisher). The acetone phase was transferred to a new tube and the extraction was repeated twice ...
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bioRxiv - Systems Biology 2024Quote: ... coupled to a Thermo Scientific Q Exactive Plus hybrid quadrupole-Orbitrap mass spectrometer (ThermoFisher), as detailed previously (52) ...
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bioRxiv - Systems Biology 2024Quote: ... Genomic DNA was extracted using DNA release from ThermoFisher Scientific.
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bioRxiv - Systems Biology 2024Quote: ... restriction enzyme then 5’ overhangs were filled in by dNTPs with Biotin-14-dCTP (Invitrogen) using DNA Polymerase I and Large (Klenow ...
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bioRxiv - Systems Biology 2024Quote: ... Libraries were quantified by NanoDrop (Thermo Scientific) and Bioanalyzer (Agilent) ...
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bioRxiv - Systems Biology 2024Quote: ... and 200 cycles per burst for 65 seconds) and biotinylated DNA fragments were pulled down using MyOne Streptavidin T1 beads (Invitrogen). End repair ...
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bioRxiv - Neuroscience 2024Quote: ... We followed the manufacturer’s protocol described in ELISA kits for measuring Aβ42 (Invitrogen, Waltham, MA, USA) and p-tau (Cell Signaling ...
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bioRxiv - Neuroscience 2024Quote: ... total proteins were visualized using the Krypton Infrared Protein Stain according to the manufacturer’s instructions (Life Technologies).
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bioRxiv - Neuroscience 2024Quote: ... The brain tissues were homogenized using N-PER lysis buffer (Thermo Scientific #87792) supplemented with protease and phosphatase inhibitors without EDTA (Thermo Scientific #1861281) ...
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bioRxiv - Neuroscience 2024Quote: ... in superfrost slides (Thermo Fisher Scientific) and #1.5 coverslips.
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bioRxiv - Neuroscience 2024Quote: ... and Protease and Phosphatase Inhibitor Cocktail 100X (Thermo Fisher Scientific). Samples were lysed in ice for 10 minutes and sonicated using an Ultrasonic Processor (Hielscher Ultrasound Technology) ...
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bioRxiv - Neuroscience 2024Quote: ... Protein concentration was determined by performing a bicinchoninic acid (BCA) assay (Thermo Scientific #23225) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... Total protein was extracted after tissue lysis with RIPA buffer (Thermo Fisher Scientific #89900) and Protease and Phosphatase Inhibitor Cocktail 100X (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... A 1:200 dilution of streptavidin-Alexa555 (Invitrogen®, USA) was then conjugated to the biocytin and slices were washed in PBS and mounted in Vectashield® H-1000 (refractive index = 1.44) ...
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bioRxiv - Neuroscience 2024Quote: General laboratory reagents and cell culture reagents were purchased from ThermoFisher Scientific (Waltham ...
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bioRxiv - Neuroscience 2024Quote: ... NeuroTrace Red (Thermo Scientific #N21482), diluted 1:100 in PBS ...
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bioRxiv - Neuroscience 2024Quote: ... 2 µL 0.5 µg/ µL oligo dT and 2 µL dNTP mix (10 mM stock made from individual 100 mM dNTP stocks, Invitrogen) were added to each sample followed by a 5-minute incubation at 65°C ...
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bioRxiv - Neuroscience 2024Quote: ... using SYBR® Green master mix (Applied Biosystems). Relative mRNA levels were calculated relative to alphaTub84B expression by the comparative Ct method ...
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bioRxiv - Neuroscience 2024Quote: ... and 1% Penicillin/Streptomycin (ThermoFischer Scientific) in 75 cm2 culture flasks (Nunc). Cells were maintained at 37°C and 5% CO2 in a humidified incubator and passaged every 3 days when confluent ...
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bioRxiv - Systems Biology 2024Quote: ... Cell number and viability were determined using trypan blue exclusion on an automated COUNTESS cell counter (Thermo Fisher Scientific, Waltham, MA). Purified cells were pelleted at 300xg then snap-frozen in liquid nitrogen until RNA extraction.
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bioRxiv - Cancer Biology 2024Quote: ... followed by 10 µg/mL blasticidin (Gibco). After blasticidin selection ...
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bioRxiv - Cancer Biology 2024Quote: ... and DU145 cells were cultured in phenol red-free-RPMI (Gibco, Thermo Fisher), and HEK293T cells were cultured in DMEM-high glucose (Sigma ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein A Dynabeads (Invitrogen 10001D) were added and incubated for another 2 hours ...
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bioRxiv - Cancer Biology 2024Quote: A trizol-based (Thermo Fisher) RNA precipitation method was used to isolate the total RNA from cells using the manufacturer-recommended protocol ...
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bioRxiv - Neuroscience 2024Quote: ... and then mounted with Prolong Gold Antifade Mountant (Invitrogen, catalog #P36961). This experiment was performed using tissue from mice from 3 different litters ...
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bioRxiv - Neuroscience 2024Quote: ... and mounted on Superfrost Plus Gold Glass Slides (Fisher Scientific, #22–035-813). Slides were subsequently stored at –80°C ...
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bioRxiv - Neuroscience 2024Quote: ... Equal amounts of CSF were loaded and separated by electrophoresis in a NuPAGE 4–12% Bis-Tris gel (Invitrogen #NP0322), transferred to a nitrocellulose membrane (250 mA ...
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bioRxiv - Neuroscience 2024Quote: ... GFP antibody (Mouse, ThermoFisher Scientific, Cat. No. G6539, 1:100) was used ...
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bioRxiv - Neuroscience 2024Quote: ... and Hs99999901_s1 (Eukaryotic 18s rRNA, VIC-MGB, ThermoFisher #4331182). The cycling process was carried out using the Roche LightCycler 480II ...
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bioRxiv - Neuroscience 2024Quote: ... Goat anti-rabbit with green fluorescence (Invitrogen, Alexa Fluor 488 conjugate ...
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bioRxiv - Neuroscience 2024Quote: Wheat germ agglutinin (WGA) conjugated with Alexa Fluor 488 (WGA-488; Thermo-Fisher Scientific, Invitrogen, Carlsbad, CA, USA) was injected into the TMJ to retrogradely label TMJ afferents ...
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bioRxiv - Cancer Biology 2024Quote: ... DAPI 1:1000 (Invitrogen, D1306) diluted in PBS 1x was applied for 30 min at RT for nuclei staining ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein concentration was measured from supernatant using a BCA assay kit (ThermoFisher, 23250). Depending on the target protein ...
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bioRxiv - Cancer Biology 2024Quote: ... EdU treatment was performed using the Click-IT EdU Imaging Kits (Invitrogen, C10337) by treating cells for 1h with EdU 7.5 µM ...
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bioRxiv - Cancer Biology 2024Quote: ... on nitrocellulose membrane (Invitrogen, IB23001/2), then blocked in either PBST-milk 5% (anti-ANP32E Abcam ab5993 ...
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bioRxiv - Cancer Biology 2024Quote: ... PMSF 1 mM (Thermo Fisher, 36978), Na3VO4 1 mM (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... Nuclei and MNi were stained using Hoechst solution (Invitrogen, H3570) 1:2000 in PBS 1x upon 30 min incubation at RT ...
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bioRxiv - Cancer Biology 2024Quote: ... DNA concentration of the enriched sequencing libraries was measured with the Qubit 3.0 fluorometer dsDNA HS Assay (Thermo Fisher Scientific). Size distribution of the resulting sequencing libraries was analyzed using Agilent BioAnalyzer 4200 (Agilent) ...