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Citations for Thermo Fisher :
2151 - 2200 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Anti-CD49-PerCPefluor710 (TS2/7) and anti-PD-1-PeCy7 (eBioJ105) were supplied by Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... and live/dead Aqua (Invitrogen) for 30 min at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... anti-H1.4 (Invitrogen, 702876), anti-H1.5 (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... the cell suspensions were filtered through a 70um pre-wet filter overlaid a 50mL conical and rinsed twice with ice-cold 1x HBSS -/- (Gibco #14175). The resulting suspension was spun at 400 x g for 5 minutes at 4°C ...
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bioRxiv - Immunology 2024Quote: ... The cells were resuspended in 3mL of warm complete RPMI media (Gibco RPMI #14175, 10% FBS, 1x Pen/Strep (Gibco #15140)) and counted and viability recorded using trypan-blue exclusion on an automated cell-counter (Countess™ ...
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bioRxiv - Immunology 2024Quote: ... plates were washed and developed with 50µl SuperAqua Blue solution (Invitrogen #00-4203-58). Plates were read at 405nm after 2-3min.
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bioRxiv - Immunology 2024Quote: ... were coated with goat anti-hIgG(H+L) antibody (ThermoFisher #62-8400, 1µg/ml in PBS) overnight at 4°C ...
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bioRxiv - Immunology 2024Quote: ... The cells were resuspended in 3mL of warm complete RPMI media (Gibco RPMI #14175 ...
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bioRxiv - Cancer Biology 2024Quote: ... and 100 μl of live/dead reagents (L3224, Invitrogen) was added followed by 20-30 min of incubation at room temperature in the dark ...
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bioRxiv - Cell Biology 2024Quote: ... supplemented with 10% FBS (Gibco), 50 U/ml penicillin and 50 µg/ml streptomycin (Gibco) ...
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bioRxiv - Cell Biology 2024Quote: ... HeLa cells were grown in DMEM high glucose (Gibco) supplemented with 10% FBS (Gibco) ...
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bioRxiv - Cell Biology 2024Quote: ... 50 U/ml penicillin and 50 µg/ml streptomycin (Gibco). The HeLa lines used in this study were as follows ...
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bioRxiv - Cell Biology 2024Quote: ... Nontargeting control siRNA (control) was SilencertmNegative Control No.5 (AM4642) (Predesigned – Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2024Quote: ... with provided supplemental cocktail and 10% FBS (Gibco) to reach a 15% final supplement concentration (volume/volume ...
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bioRxiv - Cell Biology 2024Quote: ... For SNX5 and SNX6 the following siRNA sequences were used: SNX5 (5′-UUAGUUUCAGCCCGAAGCAUC-3’), SNX6 (5′-UUAUGAGGUAGACGACUAAAU-3’) (Wassmer et al., 2007), VPS35 (ID 132357, AM16708) (Predesigned – Thermo Fisher Scientific). Nontargeting control siRNA (control ...
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bioRxiv - Cell Biology 2024Quote: Targeting siRNA was synthesized (Thermo Fisher Scientific) using the following DNA sequences for each target ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were washed and harvested with 0.25% trypsin-EDTA (ThermoFisher) when ∼90% confluence was reached ...
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bioRxiv - Immunology 2024Quote: A custom-made mouse Cytokine 26-Plex-assay kit was designed and purchased from Invitrogen. The kit included specific quantification of mouse Eotaxin ...
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bioRxiv - Systems Biology 2024Quote: ... Potential candidates were investigated by western blotting against the integrated V5-tag (Thermo Fisher, R960-25) with or without 500 uM 3-indoleacetic acid (IAA ...
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bioRxiv - Systems Biology 2024Quote: ... destination vectors using LR Clonase (ThermoFisher #11791100), positioning them upstream of the HIS3 and LacZ reporter genes ...
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bioRxiv - Systems Biology 2024Quote: PCR reactions were conducted using DreamTaq Polymerase (ThermoFisher EP0705) under the following conditions ...
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bioRxiv - Systems Biology 2024Quote: Komagataella phaffii GS115 (Invitrogen), a strain with histidine deficiency ...
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bioRxiv - Systems Biology 2024Quote: ... Cells were harvested 24 hours later and plated in medium containing 0.1 mg/ml G418 (Gibco) form selection of transduced cells ...
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bioRxiv - Systems Biology 2024Quote: ... Amplicons from each 96-well plate were pooled and purified using the PCR Purification Kit (ThermoFisher K310002). Each pooled sample was prepared as a single sequencing library by the Molecular Biology Core Facilities at the Dana-Farber Cancer Institute ...
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bioRxiv - Systems Biology 2024Quote: ... mouse α-V5-tag (Thermo Fisher R960-25, 1:1,000), or rabbit α-β-tubulin (Addgene ab6046 ...
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bioRxiv - Systems Biology 2024Quote: ... Yeast were resuspended in TE/LiAc with salmon sperm DNA (ThermoFisher 15632011) at a dilution of 1:10 ...
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bioRxiv - Systems Biology 2024Quote: ... NO2- and NO3- concentrations were measured spectrophotometrically with the Gallery™ Discrete Analyzer (Thermo Fisher Scientific). Acetate concentration was measured by high-performance liquid chromatography (Vanquish Core HPLC ...
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bioRxiv - Systems Biology 2024Quote: ... Confirmed entry vectors were cloned into DB-pEZY3 and pAD2μ (Walhout lab) destination vectors using LR clonase (ThermoFisher #11791100). Plasmid samples were prepared using the Endotoxin-Free Miniprep Kit (101 BIO #W210650 ...
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bioRxiv - Systems Biology 2024Quote: ... Acetate concentration was measured by high-performance liquid chromatography (Vanquish Core HPLC, Thermo Fisher Scientific) using an Aminex HPX-87H column (300 × 7.8 mm ...
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bioRxiv - Systems Biology 2024Quote: ... 490 µM L-glutamine (Gibco), 512 µM EGTA ...
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bioRxiv - Systems Biology 2024Quote: ... peptide concentration was determined utilizing a colorimetric peptide assay kit from Thermo Fisher Scientific.
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bioRxiv - Systems Biology 2024Quote: ... with 10% fetal bovine serum (Bio-Techne #S12450H) and 1% antibiotic-antimycotic (Gibco #15240062) at 37°C with 5% CO2 ...
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bioRxiv - Systems Biology 2024Quote: Samples were analyzed in triplicate using an UltiMate 3000 UHLC system coupled to a Q Exactive Plus mass spectrometer (ThermoFisher Scientific). Approximately 0.6 μg of peptides were loaded on a C18 precolumn (PepMap100 ...
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bioRxiv - Systems Biology 2024Quote: ... Color reaction was performed using SuperSignal West Pico Plus Chemiluminescent Substrate (ThermoFisher 34577).
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bioRxiv - Synthetic Biology 2024Quote: ... bacteria were transferred to liquid cultures in pre-warmed RPMI-1640 medium (Gibco) supplemented with 10% FBS at adjusted OD600nm = 0.05 and incubated with gentle agitation for 2 h at 37 °C in the presence of 5% CO2.
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bioRxiv - Systems Biology 2024Quote: ... and secondary anti-mouse IgG coupled with alkaline phosphatase (ThermoFisher Scientific), diluted according to manufacturer instructions ...
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bioRxiv - Systems Biology 2024Quote: ... Approximately 0.6 μg of peptides were loaded on a C18 precolumn (PepMap100, 5 μm, 300 Å, ThermoFisher Scientific) and separated on a PepMap RSLC C18 column (50 cm × 75 μm ...
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bioRxiv - Systems Biology 2024Quote: ... the concentration of total proteins was determined using a bicinchoninic acid (BCA) kit (Fisher Scientific) in accordance with the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2024Quote: ... Total RNA was extracted using TRIzol LS Reagent (Invitrogen) and chloroform and incubated overnight at −20°C in isopropanol ...
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bioRxiv - Systems Biology 2024Quote: ... and separated on a PepMap RSLC C18 column (50 cm × 75 μm, 2 μm, 100 Å, ThermoFisher Scientific) with a 1.5 h gradient with eluent A (Water ...
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bioRxiv - Systems Biology 2024Quote: ... HPLC-grade acetic acid (AA), fused silica capillaries (50 mm i.d., 360 mm o.d., Polymicro Technologies) were purchased from Fisher Scientific (Pittsburgh, PA). Acrylamide was obtained from Acros Organics (Fair Lawn ...
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bioRxiv - Systems Biology 2024Quote: ... C-terminally labeled with heavy lysine or arginine (ThermoFisher Scientific) with concentrations of 50 fmol/μg ...
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bioRxiv - Systems Biology 2024Quote: ... Cells were seeded at the coated chamber slides with a density 200000 cells per well and the next day four hours before imaging cells were stained with 1 µg/ml Hoechst 33342 for 30 min at 37°C and washed 3 times for 10 min each with Live Cell Imaging Solution (Invitrogen). When applicable ...
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bioRxiv - Systems Biology 2024Quote: ... Peptides was were loaded via an Acclaim PepMap 100 trap column (C18, 5 μm, 100Å; Thermo Scientific, San Jose, CA) onto a Waters BEH C18 column (100 µm × 100 mm ...
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bioRxiv - Systems Biology 2024Quote: ... and 1% penicillin/streptomycin (Gibco) at 37°C with 5% CO2 in a humidified incubator ...
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bioRxiv - Systems Biology 2024Quote: ... supplemented with 10% FBS (Gibco) and 1% penicillin/streptomycin (Gibco ...
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bioRxiv - Systems Biology 2024Quote: ... U2OS were cultured in DMEM (Gibco) supplemented with 10% FBS (Gibco ...
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bioRxiv - Systems Biology 2024Quote: ... and labeled with TMTpro mass tag reagent kit (Thermo Fisher Scientific), adding 100 µg reagent in 30 µL anhydrous ACN in a scrambled order and incubated at RT for 2 h ...
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bioRxiv - Systems Biology 2024Quote: ... then open tubes are placed into a vacuum concentrator (SpeedVac SRF110, Thermo Fisher Scientific, Waltham, United States) at 4°C until pooled fractions have fully evaporated ...
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bioRxiv - Systems Biology 2024Quote: ... The chromatographic performance was monitored with a UV detector (Ultimate 3000 UPLC, Thermo Scientific) at 214 nm ...