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Citations for Thermo Fisher :
1651 - 1700 of 10000+ citations for 2 4 Methyl 5 thiazolyl ethyl decanoate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... 2′-(or-3′)-O-(N-Methylanthraniloyl) adenosine 5′-triphosphate (mant-ATP, Thermo-Fisher Scientific, Waltham, MA, USA) was serially diluted to concentrations of 5 -15 μM in assay buffer and added to the myosin at a final concentration of 1X -1.2X the final concentration of myosin ...
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bioRxiv - Molecular Biology 2022Quote: ... 100 μl of RNase A/T1 mix (2 μg/μl, 5 U/μl, Thermo Scientific, cat#EN0551) was added to 400 μl of the cleared lysate and incubated for 20 min at room temperature ...
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bioRxiv - Immunology 2022Quote: ... blocked for 2 hours at room temperature with 1X PBS supplemented with 5% Fetal Calf Serum (Invitrogen) and 0.25% Tween-20 (Invitrogen) ...
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bioRxiv - Microbiology 2020Quote: ... 5 x 106 S2 cells were seeded in 2 mL Schneider’s Drosophila media (Thermo Fisher, Waltham, Massachusetts) supplemented with 10% heat-inactivated fetal bovine serum in 9.6 cm2 wells of a tissue culture plate and incubated at 26 °C ...
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bioRxiv - Physiology 2020Quote: ... The membrane was probed with primary antibodies for α-Tubulin (ThermoFisher 322588, clone B-5-1-2), acetylated (Sigma T7451 ...
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bioRxiv - Immunology 2020Quote: Cells were harvested from the peritoneum by lavage with 5 ml cold PBS + EDTA (2 mM, Gibco). After washing ...
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bioRxiv - Genetics 2020Quote: ... cells were passaged into differentiation media supplemented with 5-Ethynyl-2’-deoxyuridine (Life Technologies, A10044, 10 µM). On day 6 of differentiation ...
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bioRxiv - Molecular Biology 2021Quote: 5-ethynyl-2’-deoxyuridine (EdU)-incorporation assay has been performed using a commercially available kit (Invitrogen, C10340). Animals were injected intraperitoneally with 60 µl of EdU (0.5 mg/ml in 50% DMSO and 50% PBS solution ...
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bioRxiv - Developmental Biology 2021Quote: ... received two intraperitoneal (i.p.) injections of 5-ethynyl-2’-deoxyuridine (EdU) (Thermo Fisher Scientific, Cat. No.: A10044) 1mg each and 4 hours apart ...
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bioRxiv - Cell Biology 2022Quote: ... then transfected for 5 hours with indicated plasmids in 2 mL Opti-MEM (Invitrogen, Cat. #31985-088) using Lipofectamine 3000 transfection kit (Thermo Fisher ...
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bioRxiv - Cell Biology 2024Quote: Vacuolar pH alterations were detected using the 5-(and-6)-carboxy-2′,7′-dichlorofluorescein diacetate (CDCFDA, Invitrogen) probe ...
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bioRxiv - Developmental Biology 2024Quote: ... was prepared and added to 426.5 μL 2:1 (vol:vol) 0.1x Tris (Fisher Scientific, cat.# BP152-5)-ethylenediaminetetraacetic acid (EDTA ...
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bioRxiv - Biochemistry 2024Quote: ... 5 μL of PCR products was run on a 2% E-gel EX Agarose Gel (ThermoFisher #G401002) for 10 minutes according to the settings programmed into the gel system ...
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bioRxiv - Neuroscience 2023Quote: ... The BV-2 cells were cultured in DMEM supplemented with 5% heat-inactivated fetal bovine serum (Gibco) and 1% penicillin-streptomycin ...
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bioRxiv - Neuroscience 2023Quote: Pregnant dams were injected intraperitoneally at e12.5 with a solution containing 5-Ethynyl-2’-deoxyuridine (EdU, ThermoFisher) and embryos were collected 2h after ...
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bioRxiv - Cell Biology 2023Quote: ... and the sample was washed with 1X PBS for 2 x 5 min using a Pipetman (Gibco), then blocked using the blocking solution for 1 h ...
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bioRxiv - Cell Biology 2023Quote: ... at 150 × 106 cells/dish for 2 h at 37°C/5% CO2 in RPMI-1640 (Gibco) to separate adherent cells ...
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bioRxiv - Evolutionary Biology 2023Quote: We conducted all experiments using SM/5 media (Loomis and Sussman 1966) (2 g glucose (Fisher Scientific), 2 g BactoPeptone (Oxoid) ...
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bioRxiv - Neuroscience 2024Quote: ... mixed retinal cultures were loaded with 5 µM Fura-2 AM (acetoxymethyl ester, #F1221; Thermo Fisher Scientific) with 0.01% pluronic F-127 (#P3000MP ...
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bioRxiv - Developmental Biology 2024Quote: Dams were injected IP with 30 mg/kg body weight of 5-ethynyl-2’-deoxyuridine (EdU; Invitrogen) 12 hours prior to harvesting at E18.5 ...
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bioRxiv - Synthetic Biology 2024Quote: EVs (2×109 particles in 15 µL) were mixed with a 5 µL sample buffer (NP0007, Invitrogen) and heated at 70 ℃ for 10 min ...
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bioRxiv - Bioengineering 2024Quote: ... 2 µL mL-1 egg lecithin (5 mM in EtOH) and 1x chemical defined lipid concentrate (Gibco). After incubation for 2 days ...
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bioRxiv - Cancer Biology 2024Quote: ... permeabilized in 0.3% Triton X-100 for 5 min and blocked in 2% blocking solution (Thermo fisher). Cells were incubated overnight with the primary antibodies washed in PBS and secondary antibodies were applied for 1 hr before counter-staining with DAPI ...
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bioRxiv - Cell Biology 2024Quote: The EU/EdU co-labelling mix was prepared by combining 100µM BrdU (5-Bromo-2’-deoxyuridine, Invitrogen) and 500µM EU in Nematostella medium with 2% DMSO and 50mM MgCl2 ...
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bioRxiv - Biochemistry 2024Quote: ... and then loaded onto a trap column (PepMap100 C18, 300 μm × 2 mm, 5 μm; Thermo Scientific) followed by separation on an analytical column (PepMap100 C18 ...
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bioRxiv - Developmental Biology 2024Quote: ... 2% SDS and 5% glycerol supplemented with AEBSF and Halt protease and phosphatase inhibitors (Thermo Fisher Scientific). Protein concentrations were measured by BCA assay (Thermo Scientific) ...
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bioRxiv - Developmental Biology 2024Quote: ... We used Click-IT Plus 5-ethynyl-2′-deoxyuridine (EdU) AlexaFluor-555 or 488 (Thermo Fisher C10638) for all the Endoreplication labeling experiments ...
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bioRxiv - Cell Biology 2020Quote: ... 1000 μg of total protein was incubated overnight at 4 °C with 2 μg α-GFP (Life Technologies/ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... the cells were mounted in antifade mounting medium with 4′,6-diamidino-2-phenylindole (DAPI; Thermo Fisher Scientific) and visualized with IX81 confocal microscope (Olympus ...
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bioRxiv - Molecular Biology 2021Quote: ... and mounted on slides with Prolong Diamond Antifade Mountant with 4′,6-diamidino-2-phenylindole (DAPI) (Molecular Probes). Imaging was performed using a Nikon A1 Confocal microscope.
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bioRxiv - Genomics 2021Quote: ... Nuclei were sorted by gating on the DAPI peaks using a BD FACS Aria III (200,000 – 400,000 events) into a small volume of landing buffer (4% BSA in PBS, 2 U/µl Ambion RNase Inhibitor ...
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bioRxiv - Biochemistry 2020Quote: ... and 4 mM TCEP using a 2 mL 7,000 MWCO Zeba spin desalting column (ThermoFisher Scientific, Waltham MA). The desalted protein was incubated with 40 μL of 10 mM AlexaFluor-647 C2 maleimide (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: Transient transfections were performed by adding 2-3μg of the appropriate vector in conjunction with 4-6μL Lipofectamine transfection reagent (Invitrogen). Cell culture media was changed 12 hours post-transfection and replaced with fresh media ...
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bioRxiv - Neuroscience 2020Quote: ... the tissues were counterstained with DAPI (4’,6-diamidino-2-phenylindole) and mounted with Prolong Gold (Life Technologies). Fluorescence was detected using an epifluorescence microscope (Nikon Eclipse NiE).
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bioRxiv - Biochemistry 2020Quote: ... 2 mL and 4 mL glass vials and ultraclean Silicone/PTFE septa caps (Thermo Fisher, Waltham, MA, USA) were used for biology ...
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bioRxiv - Bioengineering 2020Quote: ... Cells migrating through the porous membrane are identified by staining with 4′,6-diamidino-2-phenylindole (DAPI; Invitrogen), counting five representative fields per insert ...
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bioRxiv - Neuroscience 2021Quote: ... buffered with 10 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) (Life Technology, Thermo Fisher Scientific Inc., USA) and coated with 20 μg/mL laminin (Sigma Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... The slides were washed in TNT and stained with 4’,6-diamidino-2-phenylindole (DAPI; Invitrogen, catalog # D3571), washed again ...
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bioRxiv - Cancer Biology 2021Quote: ... the cells were mounted on slide using Antifade mounting media with DAPI (4-6-diamidino-2-phenylindole; ThermoFisher). After sealing the coverslips with clear nail polish ...
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bioRxiv - Cell Biology 2021Quote: ... onto a Dionex IonPac AS11-HC column (2 mm × 250 mm, 4 µm particle size, Thermo Fisher Scientific) equipped with a Dionex IonPac AG11-HC guard column (2 mm × 50 mm ...
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bioRxiv - Cell Biology 2021Quote: ... equipped with a Dionex IonPac AG11-HC guard column (2 mm × 50 mm, 4 µm, Thermo Fisher Scientific). The column temperature was held at 30°C ...
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bioRxiv - Cancer Biology 2020Quote: ... Nuclei were stained with 4’,6-diamidino-2-phenylindole (DAPI) in ProLong Diamond Antifade Mountant (Thermo Fisher # P36971). Images were captured using a Nikon Eclipse Ti2 microscope ...
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bioRxiv - Microbiology 2021Quote: ... Cover slips were mounted using Prolong Gold Antifade reagent containing 4’,6-diamidino-2-phenylindole (DAPI; Life Technologies) and incubated overnight at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... The cellular nucleus was stained in blue using 4’,6-diamidino-2-phenylindole (DAPI, Fisher Scientific, Illkirch, France). Uninfected DPSCs were used as negative controls.
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bioRxiv - Cell Biology 2021Quote: ... Cells were permeabilized in FACS Buffer (DPBS without calcium and magnesium, 4% FBS, and 2 mM EDTA (Gibco)) with 0.5% (w/v ...
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bioRxiv - Cell Biology 2021Quote: ... Nucleus staining reagent 4’,6-diamidini-2-phenylindole (DAPI, #D1306) and Hoechst33342 (#H21492) were purchased from Thermo Fisher Scienfitic.
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bioRxiv - Cell Biology 2021Quote: ... Coverslips were mounted using prolonged diamond anti-fade mounting media containing DAPI (4, 6-diamidino-2-phenylindole; Invitrogen) and imaging was done on Nikon laser scanning confocal microscope Plan Apo VC 100X (oil ...
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bioRxiv - Cell Biology 2022Quote: ... samples were incubated for 30 min at 37°C with the secondary antibody diluted in PBS/3% BSA/0.05% Tween 20 (see antibodies section) and 4’,6-diamidino-2-phenylindole (DAPI 0.125 mg/ml, Invitrogen) in PBS/3% BSA/0.05% Tween 20 ...
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bioRxiv - Biochemistry 2021Quote: ... Coverslips were mounted on slides with proLong gold antifade reagent with 4’,6-diamidino-2-phenylindole (Invitrogen, USA), which was used to probe for the nucleus.
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bioRxiv - Microbiology 2022Quote: ... the cells were rinsed in PBS and the nucleus stained with 4′,6-diamidino-2-phenylindole (DAPI, ThermoFisher). Coverslips were then mounted and imaged with the Zeiss Laser Scanning 710 confocal microscope images were processed using Zeiss Zen 2011 software.