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Citations for Thermo Fisher :
1801 - 1850 of 2701 citations for 7 METHYL CAMPTOTHECIN since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... All reactions were run in a Quant Studio 7 Flex Thermal Cycler (Applied Biosystems, Life Technologies, Eugene, OR, USA). The primer efficiencies were ≥ 95% for all primer pairs ...
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bioRxiv - Neuroscience 2022Quote: ... All reactions were run in a Quant Studio 7 Flex Thermal Cycler (Applied Biosystems, Life Technologies, Eugene, OR, USA). The primer efficiencies were ≥ 95% for all primer pairs ...
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bioRxiv - Cell Biology 2022Quote: ... neurons (5-7 DIV) were transfected with 0.35–1.5 μg of total plasmid DNA using Lipofectamine 2000 Transfection Reagent (ThermoFisher, 11668030) and incubated for 18-24 h.
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bioRxiv - Bioengineering 2022Quote: COS-7 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, high glucose, GlutaMAX Supplement, pyruvate, Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... Huh-7 (Nakabayashi et al., 1982) and Huh-7.5 (Blight et al., 2002) were cultured in DMEM (Gibco 11965118) supplemented with 10% FBS and non-essential amino acid solution ...
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bioRxiv - Immunology 2022Quote: ... MCF-7 (ATCC HTB-22) breast cancer cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM, Gibco, 11960-051) containing 10% FBS ...
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bioRxiv - Immunology 2022Quote: ... Supernatant was collected for 7 days and the antibody was purified using a protein A column (ThermoFisher scientific, # 89960). Ligand binding affinity and sensitivity were assessed using ELISA techniques.
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bioRxiv - Cell Biology 2022Quote: ... Coverslips with stained cells were mounted to a glass slide with 7 µl Fluoromount-G (Invitrogen, 00-4958-02). They were sealed with nail polish within 10 minutes after mounting ...
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bioRxiv - Cell Biology 2022Quote: ... Blocking buffer was replaced after one hour with 7 µg/mL mouse anti-occludin antibody (cat# 33-1500, Invitrogen) in PBS with 5% goat serum ...
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bioRxiv - Neuroscience 2020Quote: ... RT-qPCR was performed on the ViiA 7 Real-Time PCR System (Applied Biosystems/Thermofisher, Foster City, CA, USA) using GoTaq qPCR master mix (Promega ...
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bioRxiv - Neuroscience 2020Quote: ... RT-qPCR was performed on the ViiA 7 Real-Time PCR System (Applied Biosystems/Thermofisher, Foster City, CA, USA) using GoTaq qPCR master mix (Promega ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were washed with PBS and re-suspended in 0,2 % BSA in Dulbecco’s PBS with Sytox Blue or 7-AAD (LifeTechnologies, Thermo Fisher) to exclude dead cells ...
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bioRxiv - Bioengineering 2020Quote: ... gondii GT1 T NCBI Genome assembly version 7/19/2013 genome references with ERCC sequences (obtained from ThermoFisher website). The raw fastq files from sequencing are aligned to the concatenated genomes with STAR aligner (version 2.6.0c ...
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bioRxiv - Cancer Biology 2020Quote: ... and NEAA and insulin as described above for MCF-7 cells and phenol-red free RPMI (Thermo Fisher Scientific), 10% charcoal-stripped fetal bovine serum ...
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bioRxiv - Neuroscience 2021Quote: ... The culture medium was changed every 2 to 3 days and the cells were split every 5 to 7 days using 0.25% Trypsin-EDTA (Gibco), up to 20 times ...
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bioRxiv - Immunology 2020Quote: ... Anti-mouse monoclonal antibodies targeting cell surface antigens included anti-GL7 (FITC, PE, eFluor660; clone GL 7; Thermo Fisher), anti-B220 (PE-Cy7 ...
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bioRxiv - Microbiology 2022Quote: ... or in 2 ml liquid MSgg with 7-d-old tomato seedlings in a 6-well plate (Thermo Scientific). Each well contained one seedling ...
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bioRxiv - Immunology 2022Quote: ... Single-cell suspensions were obtained by pushing the tissue through a 70 µm cell strainer and subsequently cultured at 7 × 106 cells/ml in stimulation medium consisting of RPMI1640 supplemented with 0.5% penicillin/streptomycin (Gibco), 10% FCS (Gibco) ...
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bioRxiv - Immunology 2022Quote: ... HuH-7 cell line was obtained from JCRB Cell Bank and cultured in DMEM (Gibco, catalog no. 31885-023) supplemented with 10% FBS ...
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bioRxiv - Microbiology 2022Quote: ... qPCR analysis was carried out in duplicate or triplicate on a Viia 7 real-time PCR system (Thermo Fisher). Fold changes in gene expression relative to either input (0hpi ...
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bioRxiv - Synthetic Biology 2020Quote: COS-7 cells were plated on poly-L-lysine coated coverslips and transfected using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2019Quote: ... cDNA quantification was done by real-time PCR on an ViiA 7 real-time PCR system (Thermo Fisher Scientific) using Maxima SYBR Green/ROX qPCR Master Mix (K0222 ...
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bioRxiv - Neuroscience 2019Quote: ... Plasmids pCAG-dsRed and Pak3K297R transfection was performed at 7 d in vitro (DIV) with 1 µg of DNA and 1.5 µl of Lipofectamine (Invitrogen), as described in manufacturer’s manual (Invitrogen) ...
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bioRxiv - Cancer Biology 2019Quote: ... was used for qRT-PCR in a QuantStudio 7 Flex Real-Time PCR System (Thermo Fisher Scientific, Waltham, USA). Data were analyzed by QuantStudio Software V1.3 (Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Embedded samples were cut serially into slices of 7 µm using a MICRO HM355 (Thermo Fisher Scientific, Walldorf, Germany) and stained with Hematoxylin ...
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bioRxiv - Physiology 2020Quote: ... qPCR was run in a 384-well plate format on a ViiA 7 Real-Time PCR System (Applied Biosystems) using 2x SYBR Green PCR Master Mix (Applied Biosystems) ...
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bioRxiv - Physiology 2019Quote: ... 10 μm thickness muscle cryosections were incubated with 5 μM of 2’,7’-dichlorodihydrofluorescein diacetate (DCFH; Molecular Probes, Eugene) and allowed to dry overnight at room temperature in dark ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: Liver-Chips were stained in the upper channel with 5(6)-Carboxy-2′,7′-dichlorofluorescein diacetate (CDFDA) (Thermo Fisher) to visualize bile canaliculi and MRP2 activity ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were then plated at 1.6 × 106 cells per 6-well and allowed to differentiate for 5-7 days in RPMI-1640 medium (GIBCO), 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Pathology 2019Quote: The infected rice sheaths were incubated with CellTracker™ Blue CMAC Dye (7-amino-4-chloromethylcoumarin, Molecular Probes, C2110) at a final working concentration of 10 μM for 2 h at 37 °C ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... both Huh-7 cells and PHH were processed with the TaqMan Fast Advanced Cells-to-Ct Kit (Invitrogen, A35378), according to the manufacturer’s protocol ...
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bioRxiv - Physiology 2019Quote: ... 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Thermo Fisher Scientific, USA) was dissolved in PBS at 5 mg/ml ...
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bioRxiv - Cell Biology 2020Quote: Bone marrow cells were isolated and cultured for 7±9 d in RPMI-1640 medium (Gibco, ThermoFisher, Waltham, MA) supplemented with 10% low endotoxin defined FBS (Hyclone ...
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bioRxiv - Cell Biology 2020Quote: Bone marrow cells were isolated and cultured for 7±9 d in RPMI-1640 medium (Gibco, ThermoFisher, Waltham, MA) supplemented with 10% low endotoxin defined FBS (Hyclone ...
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bioRxiv - Bioengineering 2020Quote: ... the cell pellets were incubated for an hour in 2 uM 2’,7’-dichlorodihydrogen-fluorescein diacetate (H2DCF-DA, Invitrogen) in phenol red free media (Sigma ...
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bioRxiv - Immunology 2020Quote: ... 1×106 CAR T cells were co-cultured with γ-irradiated K562-CD19 or K562-CD19-PD-L1 at 1:3 effector:target (E:T) ratio for 7 days and counted with the countess II Automated Cell Counter (ThermoFisher, USA).
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bioRxiv - Molecular Biology 2021Quote: ... HEK293 and Huh-7 cells were transfected with plasmid DNAs by standard calcium phosphate precipitation and Lipofectamine 3000 (Invitrogen), respectively.
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bioRxiv - Microbiology 2020Quote: ... Amplification of the extracted DNA template was performed in QuantStudio™ 7 Flex Real-Time PCR System (Applied Biosystems) by initial incubation of 2 min at 55°C and 10 min at 95°C ...
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bioRxiv - Immunology 2020Quote: ... Glucose uptake assay was performed by incubating cells with 50 µM 2-NBDG (2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose) (Invitrogen) for 90 min at 37°C prior to cell staining ...
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bioRxiv - Cell Biology 2019Quote: ... The plasmids encoding for His-SEPT2 or His-SEPT2-mCherry and SEPT6/7-strep were co-transformed into E.coli BL21 (DE3) (Invitrogen). Bacterial cultures were grown to OD600 of 2-3 and induced with 1 mM IPTG for 1h at 37°C (His-SEPT2 ...
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bioRxiv - Biochemistry 2019Quote: ... 7 μl of Superscript III reverse transcription master mix (containing 4 μl of 5x First Strand Buffer (Life Technologies), 1 μl of 100 mM DTT ...
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bioRxiv - Genetics 2020Quote: ... qRT-PCR assays were performed in technical duplicates in 96- or 384-well plates on QuantStudio 7 (Life Technologies) or Bio-Rad CFX 96 instrument ...
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bioRxiv - Cell Biology 2021Quote: ... cells were stained with 250 μM Cell Tracker Blue CMAC (7-amino-4-chloromethylcoumarin) dye (Life Technologies, Carlsbad, CA). Cells were then plated onto 35 mm glass bottom microwell dishes that were poly-d-lysine coated (MatTek Corporation ...
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bioRxiv - Genomics 2020Quote: ... These mixtures were measured in triplicate for each sample-primer combination with a ViiA 7 qPCR machine (Thermo Fisher)
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bioRxiv - Molecular Biology 2021Quote: ... Lysosomal ExRai AMPKAR was made by inserting PCR-amplified LAMP149 generated using primers 7-8 into HindIII (ThermoFisher FD0504)/BamHI-digested ExRai AMPKAR backbone ...
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bioRxiv - Microbiology 2020Quote: ... bone marrow derived monocytes cells were incubated for 7 days in presence of RPMI medium-Glutamax (61870-036; Gibco) supplemented with 10% heat-inactivated FBS (16140071 ...
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bioRxiv - Microbiology 2020Quote: ... Huh-7 .5 and BHK-21 cells were cultured in Dulbecco’s minimal essential medium (DMEM) (Gibco /Thermo Fisher Scientific) supplemented with 2mM L-glutamine (Gibco /Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... Huh-7 .5 and BHK-21 cells were cultured in Dulbecco’s minimal essential medium (DMEM) (Gibco /Thermo Fisher Scientific) supplemented with 2mM L-glutamine (Gibco /Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2019Quote: Huh-7 and C6/36 cells were grown in 8 well chambers (LAB-TEK®, Nalgene Nunc International, USA) for 24h ...
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bioRxiv - Immunology 2020Quote: ... Lenti-X 293T and COS-7 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Thermo Fisher Scientific, USA) supplemented with 10% Fetal Bovine Serum (FBS ...