Labshake search
Citations for Thermo Fisher :
1601 - 1650 of 2879 citations for 7 METHYL CAMPTOTHECIN since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2022Quote: ... Proliferating organoids were grown for 4–7 days and dissociated using TrypLE Express Enzyme (Thermo Fisher Scientific) for 10–20 minutes in a 37°C water bath ...
-
bioRxiv - Genomics 2022Quote: ... MCF-7 cells were maintained in phenol red–free medium containing charcoal-dextran–stripped FBS (Life Technologies) at 70–80% confluency for 2 days before treatment with 50 nM E2 (SIGMA ...
-
bioRxiv - Cell Biology 2022Quote: ... Cells were passaged every 3-7 days as single cells using TrypLE™ Select CTS™ (Gibco). The ROCK inhibitor Y27632 (Fujifilm ...
-
bioRxiv - Immunology 2020Quote: ... Reactions were carried out on a QuantStudio 6 and 7 Flex Real-Time PCR System (Applied Biosystems) according to the manufacturer’s specifications ...
-
bioRxiv - Immunology 2020Quote: ... and ran in duplicate using the QuantStudio 6 and 7 Flex Real-Time PCR System (Applied Biosystems) according to manufacturer’s specifications ...
-
bioRxiv - Microbiology 2020Quote: ... For detection of effector caspase activity 20 µM CellEvent Caspase 3/7 Green Detection Reagent (ThermoFisher Scientific) was added to the infected cells prior to imaging ...
-
bioRxiv - Molecular Biology 2020Quote: Protein-Protein interactions were analyzed on 7% Tris-acetate native gels (Thermo Fisher Scientific, cat. No. EA0355BOX) by EMSA ...
-
bioRxiv - Plant Biology 2021Quote: ... 7 µL water and 10 µL Fast SYBR Green Master Mix (Thermo Fisher Scientific, Waltham, MA, USA) performed with QuantiStudio3 (Thermo Fisher ...
-
bioRxiv - Neuroscience 2022Quote: ... 7 mL of cold Dulbecco’s Phosphate-Buffered Saline with glucose and pyruvate (DPBS, Thermo Fisher Scientific, 14287072) was added into each of the 15 mL conical centrifuge tubes on top of the strained cells ...
-
bioRxiv - Microbiology 2022Quote: ... Between 160-200ng of DNA was amplified using StepOne Plus or QuantStudio 7 Flex instruments (Applied Biosystems) using the following protocol ...
-
bioRxiv - Pathology 2022Quote: ... TGFBR3 and E6 gene and GAPDH were measured by ViiA 7 Real-Time PCR system (Life Technologies) using Fast SYBR master mix (Applied Biosystems ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... qRT-PCR was carried out on a real-time PCR system (Thermo Fisher Scientific; QuantiStudio 7 Flex) using the following conditions ...
-
bioRxiv - Immunology 2022Quote: ... The real-time PCR was performed on QuantStudio 7 Flex Real-Time PCR System (Thermo Fisher Scientific). Reactions were performed in duplicates in a final reaction volume of 20 µL containing 10 µL of Power SYBR Green Master Mix (Applied Biosystems) ...
-
bioRxiv - Immunology 2022Quote: ... and the primers listed in table S1 on a ViiA 7 Real-Time PCR System (Applied Biosystems) with the following cycling parameters ...
-
bioRxiv - Physiology 2019Quote: ... RT-PCR was performed with the ViiaTM 7 Real-Time PCR System (Life Technologies, Grand Island, NY) using SYBR® Green reagent (Life Technologies ...
-
bioRxiv - Cell Biology 2019Quote: Huh-7 cell content of GSH was determined using 50 μM monochlorobimane (mBCl, Molecular Probes, Eugene, OR) in an incubation of 20 min at room temperature in the dark (74) ...
-
bioRxiv - Genetics 2019Quote: ... All qPCR reactions were carried out using QuantStudio™ 7 Flex Real-Time PCR System (Applied Biosystems). All qPCR analysis took place in University College Dublin.
-
bioRxiv - Cancer Biology 2019Quote: ... Taqman® assays (Supplementary table 3) and QuantStudio™ 7 Flex Real Time PCR system (ThermoFisher, USA), and relative expression levels determined using QuantStudio™ 7 Real Time PCR software.
-
bioRxiv - Neuroscience 2019Quote: ... unc-7 rescue plasmids were made using the Multisite Gateway® 3-Fragment Vector Construction Kit (Invitrogen). A 1078bp mec-4 promoter fragment (as previously used ...
-
bioRxiv - Developmental Biology 2019Quote: Posterior midguts of 7 day-old adult flies were directly homogenized in 1 ml TRIzol (Life Technologies) and total RNA was isolated according to the manufacturer’s protocol (miRNeasy mini kit ...
-
bioRxiv - Cell Biology 2020Quote: ... qPCR was performed on QuantStudio™ 7 Flex Real-Time PCR System (Applied Biosystems, Foster City, CA). Cycling conditions were as follows ...
-
bioRxiv - Genetics 2019Quote: ... The reactions were run on a QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems – Thermo Fisher). Relative expression values were calculated based on the ΔΔCT method using RPS2 or GAPDH as housekeeping genes.
-
bioRxiv - Genetics 2019Quote: ... The reactions were run on a QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems – Thermo Fisher). Relative expression values were calculated based on the ΔΔCT method using RPS2 or GAPDH as housekeeping genes.
-
bioRxiv - Molecular Biology 2020Quote: ... following the vendor’s instructions on a QuantStudio 7 Flex Real Time PCR System (Life Technologies, Carlsbad, California). The PCR parameters were as mentioned above ...
-
bioRxiv - Neuroscience 2021Quote: ... Cells were harvested at DIV7 (Day 1) or DIV14 (Day 7) in N-PER (87792 Thermo Scientific) supplemented with protease inhibitors (cOmpleteTM Ultra ...
-
bioRxiv - Immunology 2020Quote: ... The real-time PCR was performed on QuantStudio™ 7 Flex Real-Time PCR System (Thermo Fisher). Reactions were performed in triplicates in a final reaction volume of 10 μl containing 5 μl of iQ™ SYBR® Green Supermix (Biorad ...
-
bioRxiv - Immunology 2020Quote: ... Viia 7 Real Time PCR system with a Power SYBR green PCR master mix kit (Applied Biosystems).
-
bioRxiv - Molecular Biology 2020Quote: ... samples were desalted using HyperSep Filter Plates with a 5-7 μL bed volume (Thermo Fisher Scientific) following the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2019Quote: ... Real time PCR step quantification was performed on ViiA 7 real time PCR system (Thermo Fisher Scientific).
-
bioRxiv - Neuroscience 2021Quote: ... The qRT-PCR runs were performed as triplicate on Applied Biosystems ViiA 7 (ThermoFisher Scientific, CA, USA). Endogenous control 18S was used as a housekeeping gene for normalisation ...
-
bioRxiv - Immunology 2021Quote: ... Reactions were carried out on a QuantStudio 6 and 7 Flex Real-Time PCR System (Applied Biosystems) according to the manufacturer’s specifications ...
-
bioRxiv - Immunology 2021Quote: ... Reactions were carried out on a QuantStudio 6 and 7 Flex Real-Time PCR System (Applied Biosystems) according to the manufacturer’s specifications ...
-
bioRxiv - Biochemistry 2021Quote: ... Freshly prepared, ice-cold RIPA buffer (110 μL, 50mM Tris, pH 7-8 (Acros Organics #14050-0010), 150 mM NaCl (Fluka #71383) ...
-
bioRxiv - Immunology 2021Quote: ... the Antibody Isotyping 7-Plex Mouse ProcartaPlex™ Panel was performed with a MAGPIX luminex reader (ThermoFisher). For total IgG and IgM quantification ...
-
bioRxiv - Neuroscience 2021Quote: ... COS-7 cells were grown in DMEM and RPE1 cells in DMEM/F12 medium (Thermo Fisher Scientific) supplemented with 10% FBS ...
-
bioRxiv - Developmental Biology 2021Quote: ... Cell between passage 4 to 7 were transfected with si-SLC38A5 siRNA (Cat# 4392420, Thermo Fisher Scientific) or negative control siRNA (si-Ctrl ...
-
bioRxiv - Biophysics 2020Quote: COS-7 (ATCC, CRL-1651) and HeLa (ATCC, CCL-2) cells were grown in DMEM (Gibco, 21063029) supplemented with 10% fetal bovine serum (Gibco ...
-
bioRxiv - Cell Biology 2021Quote: PP2 to EN transition– 7 days in BE5 medium supplemented with Betacellulin (Thermo Fisher Scientific, 20ng/ml). XXI (DNSK ...
-
bioRxiv - Cell Biology 2021Quote: ... MEF cells were supplemented with CellEvent™ Caspase-3/7 green detection reagent (Life Technologies, Darmstadt, Germany) following manufacturer’s instructions before aldehyde fixation ...
-
bioRxiv - Microbiology 2021Quote: ... or 10 μM 7-hydroxy-9H-(1,3-dichloro-9,9-dimethylacridin-2-one) succinimidyl ester (DDAO-SE) (Invitrogen) in HMI11 and incubated for 20 min at 37°C ...
-
bioRxiv - Bioengineering 2021Quote: ... Taqman runs were performed on an Applied Biosystems ViiA 7 Real-Time PCR System (ThermoFisher Scientific, USA). For gene expression analysis ...
-
bioRxiv - Microbiology 2022Quote: ... and reactions were carried out using a ViiA-7 Real-Time PCR System (Thermo Fisher, MA, USA).
-
bioRxiv - Cancer Biology 2022Quote: ... Real-time qPCR reactions were performed on a ViiA 7 Real-Time PCR System (Thermo Fisher Scientific) using PowerUP SYBR Green Master Mix (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2022Quote: ... Quantitative PCR (qPCR) analysis was conducted using a QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems). A ...
-
bioRxiv - Biophysics 2022Quote: ... 7 μL of the condensate mixtures was deposited in a silicon well (Press-to-Seal, ThermoFisher Scientific) attached on 1.5 thickness coverslips (Superior Marienfeld ...
-
bioRxiv - Microbiology 2022Quote: ... was used for relative quantification of cDNA on the ViiA 7 Real-Time PCR System (Thermo Fisher). Primer information is included in Table 1.
-
bioRxiv - Immunology 2022Quote: ... Bone marrow-derived macrophages (BMDM) were differentiated over 5-7 days in DMEM (Gibco, 11960044 or 11885092), 10% FBS ...
-
bioRxiv - Physiology 2022Quote: ... Reverse transcription PCR (RT-PCR) was performed with the Viia 7 Real-Time PCR System (Life Technologies) using SYBR Green reagent (Life Technologies) ...
-
bioRxiv - Physiology 2023Quote: ... using a dounce homogenizer then sonicated 10 times on setting 7 (Sonic Dismembrator Model 100, Fisher Scientific). The homogenate was centrifuged at 1000xg for 5 minutes at 4°C ...
-
bioRxiv - Neuroscience 2022Quote: ... 7 μL of each condensate mixture was deposited in individual silicon wells (Press-to-Seal, ThermoFisher Scientific) attached on 1.5 thickness coverslips (Superior Marienfeld ...