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Citations for Thermo Fisher :
1651 - 1700 of 2701 citations for 7 METHYL CAMPTOTHECIN since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... MEL cell line-derived clones 4D7 and 2M12 (7) were maintained in 20 µg/mL of blasticidin (Invitrogen) and 1 mg/mL of G418 (Gibco) ...
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bioRxiv - Neuroscience 2020Quote: NO production was detected by DAF-FM diacetate (4-amino-5-methylamino-2’,7’-difluorofluorescein diacetate) (D23844, ThermoFisher). Fly larvae were dissected at 24 or 48 h AI in PBS to expose the sensory neurons ...
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bioRxiv - Biophysics 2020Quote: Microinjection was performed in COS-7 cell line cultured in 10% (v/v) fetal bovine serum (Life technologies) DMEM at 1·105 cell/well density ...
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bioRxiv - Cancer Biology 2020Quote: ... BT-12 and BT-13[7] were cultured in Dulbecco’s modified Eagle’s medium with Nutrient Mixture F-12 (DMEM/F12, Gibco) supplemented with 2 mM L-glutamine ...
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bioRxiv - Cancer Biology 2021Quote: ... After 3 and 7 days of treatment cells were stained with 2% crystal violet (CV) (40583100, Acros Organics, Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... COS-7 cells (ATCC) were grown at 37°C with 5% CO2 in Dulbecco’s modified Eagle’s medium (Gibco) containing 10% fetal bovine serum and 1% penicillin/streptomycin (Invitrogen) ...
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bioRxiv - Bioengineering 2022Quote: ... were used for experiments in passage 4 to 7 and were cultivated in Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco) containing 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2022Quote: ... 5 μl of 7-amino-actinomycin D (7AAD, 50 μg/ml working solution in PBS, Thermo Fisher Scientific) was added to the stained cells 5–10 min before measurement.
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bioRxiv - Physiology 2022Quote: ... the fluorescent glucose analog 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG; 6.83 μg/kg BW, Invitrogen) dissolved in 50% dextrose (1 g/kg BW ...
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bioRxiv - Microbiology 2022Quote: ... infected Jurkat cells were initially preloaded with 5 µM of CellTracker CMAC (7-amino-4-chloromethylcoumarin) (Life Technologies) and cocultured for 6 or 24 h with MDMs plated onto coverslips ...
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bioRxiv - Bioengineering 2022Quote: ... Samples were incubated for 30 min in standard media with CellEvent Caspase 3/7 (1:400, C10423, Invitrogen) prior to imaging to observe apoptosis.
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bioRxiv - Bioengineering 2022Quote: ... COS-7 cells were plated onto Nunc Lab-Tek II Chambered Coverglass (Thermo Fisher Scientific, 12-565-335) to achieve ~70% confluence before transfection.
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bioRxiv - Neuroscience 2022Quote: ... Tax ID 10090) protein database (2017-6-7) using Proteome Discoverer (PD) 2.2 (Version 2.2.0.388; Thermo Fisher Scientific). Label-free quantification was also performed with PD 2.2 using precursor ions quantifier nodes ...
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bioRxiv - Cell Biology 2022Quote: ... and cultured for 5-7 days at P0 before been passaged with Tryspin - EDTA 0.05% (Thermo Scientific, 25300054). Cells were passaged every 4-5 days and until p8 ...
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bioRxiv - Cancer Biology 2020Quote: ... qRT-PCR assays were performed in technical quadruplicates in 384-well plates on QuantStudio 7 instrument (Life Technologies), with RT2 SYBR Green (Qiagen ...
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bioRxiv - Neuroscience 2021Quote: ... Pipettes had resistances of 5–7 MΩ when filled with this solution supplemented with Fura-2 (Molecular Probes). Recordings were made using a Multiclamp 700B amplifier (Molecular Devices ...
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bioRxiv - Microbiology 2020Quote: ... prehybridization was carried out for 2 h at 42°C in 7 mL of prehybridization buffer ULTRAHyb (Ambion). Hybridization was performed overnight at 42°C in the same buffer in the presence of a [γ-32P]-labeled DNA oligonucleotide probe ...
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bioRxiv - Microbiology 2021Quote: ... Unbound GTP was removed using 2 mL Zeba column with 7 kDa molecular weight cutoff (Thermo Scientific, 89889) and exchanged into 30 mM Tris pH = 7.5 buffer containing 1 mM DTT ...
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bioRxiv - Cancer Biology 2022Quote: ... 7% glacial acetic acid in water for 15 min and stained with GelCode Blue Stain (Thermo Fisher Scientific). For in-gel digestion ...
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bioRxiv - Molecular Biology 2022Quote: ... Quantitative Polymerase Chain Reaction (qPCR) was performed in a QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems) using SYBR Green Real-Time PCR Master mix (Invitrogen ...
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bioRxiv - Biophysics 2022Quote: ... Purification was performed using spin columns (7 kDa exclusion limit, 0.5 mL Zeba spin desalting columns, ThermoFisher Scientific). After purification ...
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bioRxiv - Cell Biology 2022Quote: Plasmids were transiently transfected into HEK293T or MCF-7 cells at 70% confluence using Lipofectamine 3000 (Invitrogen, #L3000015) in a 4:1 v/w ratio following manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: MA104-NSP5-EGFP cells (MA-NSP5-EGFP) (7) were cultured in DMEM supplemented with 10% FBS (Life Technologies), 50 μg/ml gentamycin (Biochrom AG ...
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bioRxiv - Cancer Biology 2019Quote: ... The RT-qPCR assays were completed using the ABI ViiA™ 7 sequence detection system (Applied Biosystems®) with the following parameters ...
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bioRxiv - Microbiology 2019Quote: ... Haematopoietic stem cells differentiated into BMDMs by incubating for 7 days in BMDM medium (1:1 SFM:DMEM, Gibco) supplemented with 10 % FCS (Biochrom) ...
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bioRxiv - Cancer Biology 2020Quote: ... Each murine sample was analyzed in triplicate on ViiA™ 7 Real-Time PCR System (Applied Biosystems®). Data generated was normalized to β-actin.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and Huh 7 engineering by lentivirus transduction to overexpress the human ACE2 (Huh-7hACE2) were cultured in Dulbecco’s modified 7 Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS, Gibco). Working stocks of SARS-CoV-2 ICGEB-FVG_5 isolated in Trieste ...
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bioRxiv - Neuroscience 2021Quote: ... Forty cycles of amplification were run on a Quant Studio 7 Flex Real-Time PCR System (Applied Biosystems) and the resulting data was analyzed using the comparative ΔΔCT method (Livak and Schmittgen ...
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bioRxiv - Bioengineering 2020Quote: ... WT-PGP1 were labeled with 5 μM CellTracker™ Blue 7-amino-4-chloromethylcoumarin CMAC (Molecular Probes, #C2110), iNeuron-PGP1 was labeled with 2.5 μM CellTracker™ Green CMFDA (Molecular Probes ...
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bioRxiv - Microbiology 2019Quote: ... at a final DNA concentration of 0.4 ng/μl using the ViiA 7 Real Time PCR System (ThermoFisher). Amplification conditions were as follows ...
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bioRxiv - Genetics 2021Quote: ... and expression levels were quantified in the QuantStudio(tm) 7 Flex Real-Time PCR machine (Thermo Fisher Scientific) using 2xSybRGreen Master Mix (Applied Biosystems ...
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bioRxiv - Genetics 2020Quote: ... reduced whole cell lysates were electrophoresed on a NuPAGE 7% Tris-Acetate pre-cast 1.0 mm gels (Invitrogen) for 50 minutes at 150 V and transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Developmental Biology 2020Quote: ... Cos-7 cells were transfected with 2.5μg of hexahistidine (His6) tagged TUBA1A plasmid DNA using Lipofectamine 3000 (Invitrogen). For Cos-7 proteasome inhibition assays ...
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bioRxiv - Biochemistry 2021Quote: ... ice cold PBS at 2e6 cells/ml and stained with 7-AAD viability dye (1 ug/mL, Invitrogen). Cell sorting was done on a FACSAria III instrument ...
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bioRxiv - Molecular Biology 2021Quote: ... RNAs were concentrated in an Amicon Ultra 7 kDa filter and buffered exchanged into nuclease free water (Invitrogen), to which the indicated buffer and salt concentrations were added ...
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bioRxiv - Genomics 2021Quote: ... qPCR was performed on Viia 6/7 Real time PCR system using SYBR Green master mix (Life Technologies). The primers used are listed in Supplementary table 1 ...
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bioRxiv - Molecular Biology 2021Quote: ... HepG2 and Huh-7 cells were transfected with miRNA or siRNA using LipofectamineTM RNAiMAX transfection reagent (ThermoFisher Scientific) according to the manufacturer’s instructions and following experiments were performed 48 h after transfection ...
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bioRxiv - Biophysics 2021Quote: ... Cells were finally switched to serum-reduced medium comprising DMEM (HEK293T, COS-7) or FluoroBrite DMEM (Gibco, #A1896702) (HeLa) ...
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bioRxiv - Cell Biology 2021Quote: ... These experiments were carried out with the QuantStudio 6 and 7 Flex Real-Time PCR Systems (Applied Biosystems). Expression of circRNAs was calculated relative to the housekeeping genes 18S or ACTB ...
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bioRxiv - Cell Biology 2020Quote: ... Enteroids were passaged every 5 to 7 days by incubating in 0.25% trypsin 0.5 mM phosphate buffered saline-ethylenediaminetetraacetic acid (PBS-EDTA, Gibco) solution for 3 minutes at 37°C ...
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bioRxiv - Immunology 2021Quote: ... Whole bone marrow cultures were then cultured in 10 cm non-tissue culture treated dishes for 7 days in Dulbecco’s modified Eagle’s medium (DMEM) containing 25 mM glucose (Invitrogen) and supplemented with 100 U/ml penicillin and 100 ng/ml streptomycin (Sigma) ...
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bioRxiv - Immunology 2021Quote: ... bone marrow monocytes were immuno-magnetically isolated and culture for 7 days in Dulbecco’s modified Eagle’s medium (DMEM) containing 25 mM glucose (Invitrogen) and supplemented with 100 U/ml penicillin and 100 ng/ml streptomycin (Sigma) ...
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bioRxiv - Immunology 2020Quote: ... polyclonal CD8+ T cells were labelled with 20 μM CellTracker™Blue CMAC (7-amino-4-chloromethylcoumarin; Invitrogen) for 20-30 min at 37° C ...
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bioRxiv - Immunology 2021Quote: ... Real-time qPCR reactions were performed using the QuantStudio™ 7 Flex Real-Time PCR System (Applied Biosystems).
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bioRxiv - Immunology 2020Quote: ... splenic NP366-374 TMEM were assessed with CellEvent™ Caspase-3/7 Green Flow Cytometry Assay kit (ThermoFisher). Lung single cells were stained with surface markers then incubated with caspase 3/7 green detection reagent for 30 minutes at 37°C as described in the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... siRNAs (3μl of 10μM conc.) and RNAiMax (7 μl) were mixed separately with 75 μl Optimem (Thermofisher, 31985062). Knockdown was initiated by mixing both siRNA and RNAiMAX suspensions together ...
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bioRxiv - Cell Biology 2022Quote: ... Nuclei were counterstained using 4′,6-diamidin-2-phenylindol (DAPI, 1:1000, 7 min; D1306, Thermo Fisher Scientific). Washing steps were performed with PBS ...
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bioRxiv - Cell Biology 2022Quote: WT organoids passage 10 were collected 5-7 days after passaging and digested with TrypLE (Thermo Fisher Scientific) for 20 min at 37 °C ...
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bioRxiv - Neuroscience 2022Quote: Hippocampal neurons were transfected at 7 days in vitro (DIV) using Lipofectamine 2000 (Thermo Fisher, Cat. no. 11668019). Experiments were carried out after 20-24 hours ...
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bioRxiv - Cancer Biology 2022Quote: ... with 7 to 14 cycles of PCR using the Phusion High-Fidelity PCR Master Mix (Thermo Fisher Scientific). The ctDNA yield was quantified with the Qubit™ dsDNA HS Assay Kit (Thermo Fisher Scientific) ...