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Citations for Thermo Fisher :
1851 - 1900 of 2701 citations for 7 METHYL CAMPTOTHECIN since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Supernatant was collected for 7 days and the antibody was purified using a protein A column (ThermoFisher scientific, # 89960). Ligand binding affinity and sensitivity were assessed using ELISA techniques.
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bioRxiv - Microbiology 2021Quote: ... All strains were grown on Columbia agar supplemented with 7% (v/v) sheep blood (Oxoid™, Thermo Fisher Scientific) overnight at 37°C under aerobic conditions ...
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bioRxiv - Cell Biology 2022Quote: ... 2007) and DsRed2-Mito (Tanaka Bio) plasmids using a ratio of 7:3 using Lipofectamine LTX Plus (ThermoFisher, 15338100) for 5 hours ...
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bioRxiv - Molecular Biology 2022Quote: ... The formaldehyde supernatant was removed and the pellet was resuspended in 1 mL cold molecular grade 100 mM Tris HCl pH 7 plus 0.1 U/uL SUPERase- In RNase inhibitor (ThermoFisher) to quench the formaldehyde ...
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bioRxiv - Cancer Biology 2022Quote: ... and then cultured on Petri culture dishes for 7 days in Iscove’s Modified Dulbecco’s Medium (IMDM, Gibco 12440-053) supplemented with 10% FBS ...
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bioRxiv - Bioengineering 2022Quote: ... 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (16:0 NBD) were purchased from Invitrogen. Phosphate-buffered saline (PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... alongside aforementioned activating proteins and CellEvent™ Caspase-3/7 Green Detection Reagent (1:5000, ThermoFisher Scientific, Cat#C10423). Cells were imaged using the IncuCyte ZOOM System (Essen Bioscience ...
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bioRxiv - Biophysics 2022Quote: ... HEK 293 cells and COS-7 cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Thermo Fisher Scientific, USA) supplemented with glucose (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: Cell viability after 1 and 7 days was examined using Calcein AM (Thermo Scientific, Breda, the Netherlands, CCID: 390986) for live cells and propidium iodide (PI ...
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bioRxiv - Developmental Biology 2022Quote: ... hiPSC at day 6-7 after passaging were treated with a 1:1 mixture of TrypLE Select (Life Technologies) and 0.5 mM EDTA/phosphate-buffered saline (PBS ...
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bioRxiv - Immunology 2022Quote: ... in the presence of T cells at a 1:1 effector:target cell ratio in the presence of the caspase-3/7 Green Detection Reagent (Invitrogen). Images were taken every 2 h at 10× magnification ...
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bioRxiv - Microbiology 2022Quote: ... then 7 µg of protein along with PageRuler Prestained ladder 10 kDa to 180 kDa (26616, Thermo Fisher Scientific) was loaded on a NuPage 4-12% gradient Bis-Tris pre-cast SDS-PAGE gel (NP0321 ...
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bioRxiv - Neuroscience 2022Quote: ... of 0.5% low-melting agarose (FMC) containing a 6:7 ratio of spinal cord medium (MEM with Glutamax (Gibco) supplemented with 4 mg/ml Albumax (Gibco) ...
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Vitamin D signaling orchestrates skeletal muscle metabolic flexibility by regulating its fuel choicebioRxiv - Physiology 2023Quote: ... Quantitative RT– PCR was performed using the QuantStudio-6 and -7 Flex Real-Time PCR Systems (Thermo Fisher Scientific) with SYBR® Premix Ex Taq (Tli RNase H Plus ...
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bioRxiv - Immunology 2022Quote: ... cells were resuspended in glucose-free media containing 150 μM 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG Thermofisher) and incubated for 45 minutes at 37°C.
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bioRxiv - Molecular Biology 2022Quote: ... p2×7 (Mm00440582_m1) and tgfβ (Mm01178820_m1) were determined by quantitative PCR using Taqman primers and probe (Applied Biosystems, USA) and Taqman Gene Expression Master Mix (Applied Biosystems ...
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bioRxiv - Molecular Biology 2022Quote: ROS levels were detected using the chloromethyl-2’,7’-dichlorodihydrofluorescein diacetate (CM-H2DCFDA) general oxidative stress indicator kit (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... pH 7.4) in a pre-chilled 7 mm x 20 mm thick-walled polycarbonate ultracentrifuge tubes (Thermo Scientific # 45233) and centrifuged in a S100AT3 rotor at 4°C for 60 min at 50,000 x g (43,000 rpm ...
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bioRxiv - Microbiology 2022Quote: ... using qScript 1-Step SYBR Green qPCR kit (Quantabio) on a ViiA 7 Real-Time PCR System (Applied Biosystems); 16S rRNA was used for normalization ...
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bioRxiv - Biophysics 2022Quote: ... 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-ATTO647N (ATTO-lipid) and (iii) 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-Cyanine 7 (Cy7-lipid) were from Invitrogen, ATTO-TEC and Avanti Polar Lipids ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were pelleted at 3,000 g, aspirated, resuspended in SYTOX buffer (500 µL 50mM sodium citrate [pH 7], 1 µM SYTOX green (Invitrogen)) ...
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bioRxiv - Immunology 2022Quote: ... All samples were prepared in triplicate and run on a QuantStudio 6/7 Flex Real-Time PCR System (ThermoFisher). Primer sequences for designated HHV-6 marker (U31 ...
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bioRxiv - Genomics 2022Quote: ... mouse pancreatic islets isolated from RipCre-tdTomato mice were trypsinized for 7 minutes with 1 mM of trypsine (Gibco). Dissociated pancreatic cells were directly run into an Influx sorter (Becton Dickinson® ...
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bioRxiv - Neuroscience 2023Quote: ... On day 7 of neural induction cells were replated following the manufacturer’s protocol in a Geltrex-coated (Thermo Scientific) 6-well plate (500x103 cells/well ...
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bioRxiv - Pathology 2023Quote: ... We performed the LN34 and β-actin RT-qPCRs on a QuantStudio 7 Flex (ThermoFisher Scientific, Waltham, MA, USA) as described previously [13 ...
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bioRxiv - Developmental Biology 2023Quote: ... COS-7/ventral spinal cord cocultures were grown for 44-48 hours in motor neuron media (Neurobasal-A (Gibco), 2% B-27 ...
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bioRxiv - Cancer Biology 2023Quote: ... normalized to the expression of GAPDH on a QuantStudio 6 and 7 Pro real-time PCR system (Applied Biosystems).
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bioRxiv - Cell Biology 2023Quote: ... Results were analyzed with the Design and Analysis Software QuantStudio 6/7 Pro systems (Thermo Fisher Scientific, version 2.6). Primers 20-23,36-39 (Supplementary Methods Table 2 ...
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bioRxiv - Cell Biology 2023Quote: ... Results were analyzed with the Design and Analysis Software QuantStudio 6/7 Pro systems (Thermo Fisher Scientific, version 2.6). Primers 20-59 (Supplementary Methods Table 2 ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by another 7 days of culture with RPMI B27+ (RPMI 1640 GlutaMAX + 2% B27 supplement with insulin (ThermoFisher), 200 μM L-ascorbic acid (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were transferred onto a nitrocellulose membrane using iBlot 2 gel transfer device (7 min at 25 V, ThermoFisher). Membranes were incubated for 1 hour at room temperature (RT ...
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bioRxiv - Systems Biology 2023Quote: ... Relative transcript quantifications were done by real-time PCR on an ViiA 7 Real-Time PCR System (Thermo Fisher) using Maxima SYBR Green/ROX qPCR Master Mix (K0222 ...
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bioRxiv - Neuroscience 2023Quote: Organ of Corti explants were dissected at postnatal day 4 through 7 (P4-P7) in Leibovitz’s L-15 cell culture medium (Invitrogen), containing the following inorganic salts (in mM) ...
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bioRxiv - Neuroscience 2023Quote: ... Comparative gene expression of sgsh/CG14291 or hgsnat/CG6903 was determined on a QuantStudio 7 Flex (Thermo Fisher Scientific). Reactions were performed in triplicate in 384-well plates using Platinum SYBR Green qPCR SuperMix-UDG with ROX (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2023Quote: ... Quantitative RT-PCR was performed on a ViiA 7 Real-Time PCR System with OptiFlex Optics System (Applied Biosystems) using PowerUp SYBR Green PCR kit (Applied Biosystems) ...
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bioRxiv - Immunology 2023Quote: ... we added 7 µl of the RT mix that contained 0.5 µl of 200 U/µl Superscript IV (Invitrogen), 0.25 µl of 40 U/µl RNAseOUT (ThermoFisher Scientific) ...
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bioRxiv - Biochemistry 2023Quote: ... sample was buffer exchanged into 100 mM ammonium acetate using ZebaTM spin column (7 kDa MWCO, Thermo Fisher Scientific). Sample was loaded into Au/Pd-coated borosilicate emitters (Thermo Scientific ...
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bioRxiv - Immunology 2023Quote: ... and buffer exchange was performed with a 7 kDa molecular weight cut-off size exclusion resin (Thermo Fisher Scientific) to cryo-EM buffer (10 mM Tris at pH 7.6 and 20 mM NaCl).
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bioRxiv - Biophysics 2023Quote: ... The reaction mixture was then transferred to 2 ml ZebaSpin MWCO 7 kD desalting column (Thermo Fisher Scientific, USA) to separate the labeled protein from the free dye ...
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bioRxiv - Cancer Biology 2022Quote: ... 7*103 single cells derived from second-generation spheres were labeled with 1 mM CMFDA (Life Technologies, cat# C7025) for 30 minutes and added on top of the mesothelial monolayer ...
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bioRxiv - Developmental Biology 2023Quote: ... (7) Mount tissues on glass slides (Superfrost Plus Microscope slides, Fisherbrand) using ProLong Diamond Antifade Mountant containing DAPI (Invitrogen) and store slides at 4µC overnight ...
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bioRxiv - Bioengineering 2023Quote: Human breast cancer cell lines MDA-MB-231 (ATCC) and MCF-7 (ATCC) were grown in DMEM/F12 (Gibco) with 15 mM HEPES supplemented with 10% v/v fetal bovine serum (VWR ...
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bioRxiv - Neuroscience 2023Quote: ... The qPCR analysis was performed with a QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems; Thermo Fisher Scientific) using the ΔΔCt method [50] ...
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bioRxiv - Neuroscience 2023Quote: ... The qPCR analysis was performed with a QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems; Thermo Fisher Scientific) using the ΔΔCt method [50] ...
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bioRxiv - Physiology 2023Quote: ... Quantitative real-time PCR was carried out on a ViiA 7 instrument (Thermo Fisher Scientific, Waltham, MA 02451, USA) using HOT FIREPol Probe mix Universal (Solis Biodyne ...
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bioRxiv - Molecular Biology 2023Quote: ... miRNAs were then quantified using individual TaqMan assays on an ABI QuantStudio 7 real time PCR machine (Applied Biosystems). Assay IDs include ...
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bioRxiv - Neuroscience 2023Quote: ... Peptide eluents were separated on a self-packed C18 (1.7 µm Water’s BEH) fused silica column (laser pulled 15 cm × 150μM ID) by Ultimate 3000 RSLCnano (Thermo Scientific). Elution was performed over a 36-min gradient at a rate of 1ul/min with buffer B ranging from 3% to 50% (buffer A ...
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bioRxiv - Cell Biology 2024Quote: Cells cultured for 4 days at a starting density of 7 x 104/cm2 were lysed in TRIzol (Ambion) for 5 min and transferred to an RNA-free microcentrifuge tube following manufacturer recommendations ...
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The effect of single mutations in Zika virus envelope on escape from broadly neutralizing antibodiesbioRxiv - Microbiology 2023Quote: ... the transfection mixture was replaced with low-glucose DMEM supplemented with 7% FBS (Gibco BRI; Life Technologies, Gaithersburg, MD). Cells were then cultured for 4-6 days at 30°C and 5% CO2 ...
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bioRxiv - Cancer Biology 2023Quote: A549 cells grown in 96-well plates were stained with CellEvent Caspase 3/7 green detection reagent (Thermo Scientific) according to manufacturer’s instructions and the fluorescence measured using a plate reader at 488 nm wavelength ...