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Citations for Thermo Fisher :
1701 - 1750 of 2879 citations for 7 METHYL CAMPTOTHECIN since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Coverslips were mounted on microscopy slides using 7 µl ProLong Glass Antifade mounting medium (ThermoFisher Scientific, Invitrogen). The mounting medium was cured for 24 h at RT.
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bioRxiv - Molecular Biology 2023Quote: ... Coverslips were mounted on microscopy slides using 7 µl ProLong Glass Antifade mounting medium (ThermoFisher Scientific, Invitrogen). The mounting medium was cured for 24 h at RT.
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bioRxiv - Molecular Biology 2023Quote: ... Coverslips were mounted on microscopy slides using 7 µl ProLong Glass Antifade mounting medium (ThermoFisher Scientific, Invitrogen). The mounting medium was cured for 24 h at RT.
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bioRxiv - Molecular Biology 2023Quote: ... Coverslips were mounted on microscopy slides using 7 µl ProLong Glass Antifade mounting medium (ThermoFisher Scientific, Invitrogen). The mounting medium was cured for 24 h at RT.
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bioRxiv - Developmental Biology 2023Quote: ... Zebrafish cDNA was prepared from RNA extracted from AB zebrafish (1–7 dpf) using Trizol (Ambion #15596026) and phenol:chloroform (Millipore #19K0856166) ...
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bioRxiv - Synthetic Biology 2023Quote: Protein thermal stability was measured by differential scanning fluorimetry using a QuantStudio Pro 6/7 (Applied Biosystems). Protein was brought to a concentration of 10 uM with 5x Sypro Orange dye in a final volume of 20 µL in 20 mM NaPi ...
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bioRxiv - Neuroscience 2023Quote: Dead and apoptotic cells were detected using the CellEvent Caspase-3/7 Kit (#C10423, Thermo Fisher Scientific) according to the manufacturer’s recommendations ...
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bioRxiv - Developmental Biology 2023Quote: ... and carried out on an Applied Biosystems Vii 7 RT-PCR system (Thermo Scientific P/N 4453552). Validated gene-specific primers in this study can be found in Table S14 ...
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bioRxiv - Cell Biology 2023Quote: ... Treated and untreated organoids were collected at day 7 and dissociated to single cells using TrypLE (Invitrogen) incubation at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... 4T1 and E0771.lmb.PuroR metastasis were cultured for 7-10 days with minimal disturbance in IMDM (Gibco) supplemented with 10% FBS ...
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bioRxiv - Cancer Biology 2023Quote: ... and transfected with indicated siRNAs (listed in Supplementary table 7) with the use of Lipofectamine RNAiMAX (Invitrogen), according to manufacturer’s instructions using Lipofectamine RNAiMAX (Invitrogen) ...
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bioRxiv - Neuroscience 2023Quote: ... The resultant reporter construct was transiently transfected into COS-7 cells using Lipofectamine LTX (Thermo Fisher Scientific), together with an internal control vector pGL4.74 (Promega ...
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bioRxiv - Plant Biology 2023Quote: ... Total RNA from 10 of 7-day-old seedlings was extracted by using TRI reagent (AM9738, Invitrogen). In total ...
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bioRxiv - Genetics 2023Quote: ... and fluorescence was monitored on ABI ViiA 7 and QuantStudio 5 Realtime PCR system (Applied Biosystems, USA). Melting curve analysis was done for each amplicon ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryos were dissected from pregnant mice at embryonic day 7.7–7.8 (wild type) or 8.0 (iv/iv) in Dulbecco’s Modified Eagle Medium (DMEM, 12320-032, Gibco) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Genomics 2023Quote: ... MCF-7 breast cancer cells were seeded for 24 hours in phenol red-free MEM medium (GIBCO) supplemented with 5% charcoal-stripped calf serum (Atlanta Biologicals) ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were resuspended in PBS with 7 μg/ml DAPI and measured on an Attune (Thermo Fisher). Data were analyzed using FlowJo software version 10.7.0.
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bioRxiv - Genetics 2023Quote: ... Single mES cells (7 × 105 cells) were transfected with vectors using Lipofectamine LTX (#15338100; Thermo Fisher Scientific) [DNA ...
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bioRxiv - Microbiology 2023Quote: ... using a QuantStudio 7 Flex Real-Time PCR System utilizing TaqMan Gene Expression Master Mix (Applied Biosystems).
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bioRxiv - Cell Biology 2024Quote: ... Real-time PCR was performed on a QuantStudio™ 7 Flex Real-Time PCR System (Applied Biosystems) after mixing cDNA ...
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bioRxiv - Cell Biology 2024Quote: ... Single cell embedded prostate organoids were supplemented with 10 μM Y-27632 (Fisher Scientific, #50-863-7) for the first 2-3 days in culture prior to change with fresh organoid media lacking Y-27632 ...
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bioRxiv - Immunology 2024Quote: ... ≈2.5x105 cells were run in each lane of a 7% NuPAGE tris-acetate gel (Invitrogen, Carlsbad, CA) and transferred to Immobilon-FL polyvinylidene difluoride membrane (EMD Millipore ...
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bioRxiv - Physiology 2024Quote: ... in the QuantStudio™ 7 Flex Real-Time PCR System (384-well block, Applied Biosystems, Cat #4485701). 18s rRNA was used as an internal reference control ...
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bioRxiv - Immunology 2024Quote: ... Relative quantification of genes of interest was performed by qPCR analysis using QuantStudio Pro 7 system (ThermoFisher) and PowerTrack SYBR Green master mix (ThermoFisher) ...
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bioRxiv - Microbiology 2020Quote: ... upon which 1 μg of yeast RNA and 10× structure buffer (0.1 M Tris at pH 7, 1 M KCl, 0.1 M MgCl2; Ambion) were added ...
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bioRxiv - Plant Biology 2020Quote: ... on 4-16% (Figures 4A and 7) or 3-12% (Figures 4C and 6) NativePAGE gels (Life technologies). Cathode Running buffer (Life technologies ...
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bioRxiv - Synthetic Biology 2021Quote: ... cultured neurons were transfected at 7 days in vitro (DIV) with a commercial calcium phosphate transfection kit (Invitrogen) as previously described49 ...
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bioRxiv - Cancer Biology 2021Quote: ... and qPCR was performed using let-7 and 2S rRNA TaqMan MicroRNA Assay primer mix (Thermo Fisher Scientific) with the TaqMan Small RNA Assay (Applied Biosystems).
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bioRxiv - Neuroscience 2021Quote: ... Larval zebrafish at 6-7 dpf were paralyzed by immersion in 1 mg/ml alpha-bungarotoxin solution (Invitrogen) dissolved in external solution (in mM ...
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bioRxiv - Cell Biology 2020Quote: Following buffer exchange to 100 mM NaHCO3 with 0.5 ml 7 kDa Spin Desalting Columns (Thermo Fisher, 89882), the anti-TFAM (TA332462 ...
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bioRxiv - Cell Biology 2020Quote: ... 7- Amino-4-Chlormethylcumarin (CMAC) was part of the Yeast Vacuole Marker Sampler Kit (Y7531) from Thermo Fisher and staining was performed according to manufacturer recommendations ...
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bioRxiv - Physiology 2019Quote: ... Malpighian tubules from 7-day-old adult female flies were dissected under Schneider’s insect medium (Thermo Fisher Scientific) and isolated into 10 μl drops of a 1:1 mixture of Schneider’s medium ...
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bioRxiv - Synthetic Biology 2019Quote: ... 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) was purchased from Invitrogen. Calcein dye ...
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bioRxiv - Cell Biology 2019Quote: ... q-PCR was performed using a ViiA 7™ Real Time PCR System (Applied Biosystems, Life Technologies, UK) programed to perform the following steps ...
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bioRxiv - Cell Biology 2019Quote: ... q-PCR was performed using a ViiA 7™ Real Time PCR System (Applied Biosystems, Life Technologies, UK) programed to perform the following steps ...
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bioRxiv - Bioengineering 2021Quote: ... Real-time polymerase chain reaction (real-time PCR) was performed on duplicates using QuantStudio 7 Flex (Applied Biosystems, Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2020Quote: ... roots of 7-day-old seedlings were incubated with 50 µM Amplex™ UltraRed Reagent (AUR, Molecular Probes) for 20 min in dark conditions and rinsed with liquid MS ...
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bioRxiv - Microbiology 2021Quote: ... mRNA expression of the indicated genes was analyzed by QuantStudio 7 Flex Real Time PCR System (Applied Biosystems) using SYBR green master mix ...
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bioRxiv - Microbiology 2020Quote: ... Vero E6 and Huh-7 cells (Apath LLC, Brooklyn) were maintained in Dulbecco’s modified eagle medium (DMEM, Gibco) supplemented with 10 % heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Bioengineering 2021Quote: EV-rich fractions (7-10) from SEC were initially determined by protein (Pierce™ BCA Protein Assay, ThermoFisher) and particle quantification using nanoparticle tracking analysis (NTA ...
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bioRxiv - Bioengineering 2021Quote: ... Apoptosis was detected by incubating devices with 5μM of CellEvent™Caspase 3/7 Green detection reagent (Invitrogen) for 30 minutes at 37°C.
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bioRxiv - Biochemistry 2020Quote: ... Hs00961560_m1 Hs INSR) and the QuantStudio™ 7 Flex Real-Time PCR system (Thermo Fisher Scientific, MA, USA) and results analysed by the comparative Ct method ...
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bioRxiv - Cancer Biology 2020Quote: ... were used to analyse mRNA expression levels using a QuantStudio 7 Flex RT PCR machine (Thermo Fisher Scientific). TaqMan probes used were FAP (Hs00990791_m1) ...
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bioRxiv - Physiology 2021Quote: ... Reactions were performed in a total volume of 10 μl on a ViiA 7 system (Thermo Fisher Scientific). Gapdh was used as a housekeeping gene and data were evaluated by conversion to ΔCt values.
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bioRxiv - Cell Biology 2021Quote: ... Both COS-7 and HEK 293T were transfected by means of Lipofectamine and PLUS reagent (Thermo Fisher Scientific). The calcium phosphate transfection method was instead used to transfect WT or ΔOLF Lphn2-HA-pCCL in HEK 293T cells and to produce the corresponding viral particles subsequently employed to transduce ECs ...
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bioRxiv - Microbiology 2021Quote: ... a 20μL reaction was loaded into a ViiA 7 Real Time PCR system (Thermo Fisher Scientific, Waltham, MA). The following conditions were used ...
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bioRxiv - Molecular Biology 2021Quote: ... quantitative RT-PCR analysis was performed using a sequence detection system (ABI ViiA 7 system; Thermal Fisher Scientific). The following primers sets were used for analysis ...
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bioRxiv - Neuroscience 2020Quote: ... 2x SYBR master mix (Thermoscientific) were used and the reaction was run in Thermocycler (Applied Biosystems ViiA 7) following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2021Quote: ... MEL cell line-derived clones 4D7 and 2M12 (7) were maintained in 20 µg/mL of blasticidin (Invitrogen) and 1 mg/mL of G418 (Gibco) ...
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bioRxiv - Neuroscience 2020Quote: NO production was detected by DAF-FM diacetate (4-amino-5-methylamino-2’,7’-difluorofluorescein diacetate) (D23844, ThermoFisher). Fly larvae were dissected at 24 or 48 h AI in PBS to expose the sensory neurons ...