Labshake search
Citations for Thermo Fisher :
2051 - 2100 of 2701 citations for 7 METHYL CAMPTOTHECIN since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2022Quote: ... Bone marrow-derived macrophages (BMDMs) and dendritic cells (BMDCs) were generated for 7 days in respectively IMDM or RPMI-1640 medium (Gibco), supplemented with glutamine ...
-
bioRxiv - Immunology 2022Quote: ... 5-7 × 106 cells were resuspended in 3 mL of FACS buffer (4% FBS in phosphate buffered saline (PBS, Gibco)) and sorted at the University of Massachusetts Amherst Flow Cytometry Core Facility using a BD FACSAria Fusion (Becton Dickinson) ...
-
bioRxiv - Plant Biology 2022Quote: ... Entry clones were then introduced into the destination vector pH-Ubi-Cas9-7 which also encodes the Cas9-enzyme (Miao et al., 2013) using LR recombination (Invitrogen). T1 CRISPR/Cas9 lines were genotyped for the absence of the T-DNA construct by using the primers priMR410/411 (Hyg-1F/Hyg-1R) ...
-
bioRxiv - Microbiology 2022Quote: ... TcdB protein was diluted to 0.05 µg/µL using phosphate buffer (100 mM KPO4, 150 mM NaCl, pH 7) containing 5X SYPRO Orange (Invitrogen S6650), and a serial dilution of test compound ...
-
SLC15A4 favors inflammasome function via mTORC1 signaling and autophagy restraint in dendritic cellsbioRxiv - Immunology 2022Quote: Bone marrow cells were isolated and cultured for 7-9 d in RPMI-1640 medium (Gibco, ThermoFisher Scientific, Waltham, MA) supplemented with 10% low endotoxin-FBS (Hyclone ...
-
SLC15A4 favors inflammasome function via mTORC1 signaling and autophagy restraint in dendritic cellsbioRxiv - Immunology 2022Quote: Bone marrow cells were isolated and cultured for 7-9 d in RPMI-1640 medium (Gibco, ThermoFisher Scientific, Waltham, MA) supplemented with 10% low endotoxin-FBS (Hyclone ...
-
bioRxiv - Microbiology 2022Quote: ... CellEvent™ Caspase-3/7 Green Detection Reagent (C10723) and PrestoBlue™ Cell Viability Reagent (A13261) were purchased from ThermoFisher Scientific ...
-
bioRxiv - Microbiology 2022Quote: ... using either a QuantStudio™ 7 Flex Real-Time PCR System or a StepOnePlus Real-Time PCR System (Applied Biosystems). Individual standard curves were generated specifically for each system ...
-
bioRxiv - Microbiology 2022Quote: ... Sydney Strain SS1 (cagA+, vacA s2m2) were grown on brain heart infusion (BHI) agar (Difco, USA) containing 7% heat-inactivated horse serum (Invitrogen), antibiotics and IsoVitaleX as mentioned previously (33) ...
-
bioRxiv - Microbiology 2022Quote: ... The loaded plate was amplified for 40 cycles via the ViiA 7 real-time PCR system (Thermo Fisher Scientific, Waltham). For each biological replicates (n = 3) ...
-
bioRxiv - Molecular Biology 2023Quote: ... Northern blot analysis of U1 snRNA was performed using 5’-radiolabeled DNA probes (Sequence is listed in Supplemental Table 7) in ultrasensitive hybridization Buffer (Thermofisher). The radioactivity signals were analyzed by PhosphorImager (Typhoon FLA 7000).
-
bioRxiv - Plant Biology 2023Quote: ... These cassettes were sub-cloned into a modified binary cas9 vector pH-Ubi-cas9-7 (Fayos et al., 2020; Miao et al., 2013) in a Gateway LR reaction (Invitrogen) to generate p-OsCPR5.1-A and p-OsCPR5.1-C constructs (Fig ...
-
bioRxiv - Neuroscience 2022Quote: Larval fish (4-7 dpf) were mounted in a small drop of 2% low melting point agarose (ThermoFisher Scientific 16520) in the center of the uncoated side of a mirror galvanometer (Thorlabs GVS0111) ...
-
bioRxiv - Physiology 2023Quote: ... at 1,000g for 2min and then immediately loaded into the QuantStudio 6 and 7 Flex real-time PCR system (ThermoFisher Scientific). A two-step cycling protocol was implemented to collect cycle threshold (Ct ...
-
bioRxiv - Molecular Biology 2022Quote: ... Samples were resolved by 7% SDS-PAGE and transferred to nitrocellulose membrane by semi-dry i-blot transfer system (IB1001, Invitrogen). Inputs and elution fractions were analyzed on separate gels ...
-
bioRxiv - Molecular Biology 2022Quote: ... Samples were resolved by 7% SDS-PAGE and transferred to nitrocellulose membrane by semi-dry i-blot transfer system (IB1001, Invitrogen). Inputs and elution fractions were analyzed on separate gels ...
-
bioRxiv - Cancer Biology 2022Quote: The COS-7 cells were seeded on gold-nanoparticle-coated cover glasses and cultured overnight in DMEM (11965-092, Gibco) containing 10% FBS (10082147 ...
-
bioRxiv - Synthetic Biology 2022Quote: ... 5’ and 3’ RACE ready cDNA was generated from RNA extracted from ~30 pairs of ovaries or testes dissected from 5-7 days-post-emergence (dpe) Liverpool strain adults using Trizol (Invitrogen). Primers LA1076 then nested with LA1352 (5’ ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... ABI QuantStudio (model 7 Flex) was used for quantitative PCR reactions with SYBR green master mix (Applied Biosystems; Cat #4367659). The catalog of chosen primers can be found in supplementary data (Supplementary Table 1) ...
-
bioRxiv - Neuroscience 2022Quote: ... samples were diluted to 1M urea with a blend of 468 μL of 50 mM ammonium bicarbonate (32) and 7 μg of Trypsin (ThermoFisher). The samples were subsequently incubated overnight at 25°C for digestion with trypsin ...
-
bioRxiv - Immunology 2023Quote: Lungs were transferred into 7 ml bijoux tubes containing 500 µl digestion mix consisting of 5 mg/ml collagenase I (Gibco) and 10 µg/ml DNase I (Sigma ...
-
bioRxiv - Neuroscience 2023Quote: ... Three technical replicates per reaction was performed on the QuantStudio 7 Flex Real-Time PCR system (Applied Biosystems, Life Technologies). Data was normalized to housekeeping genes (Tubulin ...
-
bioRxiv - Neuroscience 2023Quote: ... Three technical replicates per reaction was performed on the QuantStudio 7 Flex Real-Time PCR system (Applied Biosystems, Life Technologies). Data was normalized to housekeeping genes (Tubulin ...
-
bioRxiv - Biochemistry 2023Quote: ... Cells were then washed and stained for 20 min at 4 °C with the following antibodies: anti-mouse CD8α APC-efluo780 (clone 53-6-7, ebioscience/ Thermofisher), anti-mouse CD8β AF700 or BUV495 (clone YTS156 ...
-
bioRxiv - Cell Biology 2023Quote: ... qRT-PCR reactions were performed on a QuantStudio 7 Flex PCR machine using PowerUp Sybr Green Master Mix (Applied Biosystems). Corresponding changes in the expression levels of selected genes were calculated using the ΔΔCt method relative to housekeeping genes ...
-
bioRxiv - Plant Biology 2023Quote: Quantification of ROS in guard cells of epidermal peels was measured using 20 µM 2’,7’-dichlorodifluorescein diacetate (H2DCFDA; Invitrogen) according to (30) ...
-
bioRxiv - Bioengineering 2023Quote: ... Monocytes were then differentiated into macrophages by 7–9 d of culture in IMDM + 10% AB human serum (Life Technologies).
-
bioRxiv - Cell Biology 2023Quote: ... Coding sequences were amplified using total RNA from 7 days post-fertilization (dpf) zebrafish using the SuperScript IV One-step RT-PCR kit (ThermoFisher) using the primers listed in Table S1 ...
-
bioRxiv - Microbiology 2023Quote: ... Samples were heated 5 min at 95 degrees and analysed on a 7 M urea 15% acrylamide gel further stained with SYBR Gold (ThermoFisher). As a positive control ...
-
bioRxiv - Cell Biology 2023Quote: ... Cells were then dissociated using 0.5mM EDTA into a single cell suspension and incubated in 5 μM of cell-permeant 2’,7’-dichlorodihydrofluorescein diacetate (CM-H2DCFDA) dye (Invitrogen) for 30 min at 37°C in the dark ...
-
bioRxiv - Cancer Biology 2023Quote: MCF-7 and MDA-MB 231 (was a gift from Dr Annette Meeson, Newcastle University) were cultured in DMEM (GIBCO) supplemented with 10% foetal bovine serum (FBS ...
-
bioRxiv - Cancer Biology 2023Quote: ... iTaq Universal SYBR Green Supermix was used according to the manufacturer’s protocol for RT-qPCR reaction in a QuantStudio 7 Flex (Applied Biosystem by Life Technologies). qRT-PCR primers can be found in Supplementary Information Table1 ...
-
bioRxiv - Developmental Biology 2023Quote: ... and Ccnd1 was performed using SYBR Green qPCR Reagent (Quantabio) on a ViiA 7 Real-Time PCR machine (Applied Biosystems), as described above ...
-
bioRxiv - Microbiology 2023Quote: ... Glass coverslips with CFBE41o-cells grown for 7 days were affixed into the chamber with continuous flow of Minimal Essential Media (MEM) (GIBCO) without phenol red supplemented with 2 mM L-glutamine (GIBCO) ...
-
bioRxiv - Cell Biology 2023Quote: The ROS dye assay was performed using Di(Acetoxymethyl Ester) (6-Carboxy-2’,7’-Dichlorodihydrofluorescein Diacetate) ROS dye (Invitrogen, D23844), a fluorogenic dye that is converted to 6-CarboxyFluorescein ...
-
bioRxiv - Plant Biology 2023Quote: ... 7-day-old seedlings stably expressing LIP1-GFP were vacuum-infiltrated for 30 min with 200 nM MitoTracker Orange (Invitrogen). To test for colocalization seedlings were imaged on a confocal microscope (Zeiss LSM 780 ...
-
bioRxiv - Cell Biology 2023Quote: ... the COS-7 cells were transfected with plasmids encoding tubulin (3×mEmerald-ensconsin) and ER (mCherry-KDEL) by using Lipofectamine 3000 (Invitrogen) according to the manufacturer’s protocol17 ...
-
bioRxiv - Biochemistry 2023Quote: ... Cell proliferation was assessed at different time points (3, 5, 7 and 9 days) using AlamarBlue Cell Viability Reagent (Invitrogen) per manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2023Quote: ... quantitative polymerase chain reaction (qPCR) was performed using a QuantStudio 7 Flex qPCR system (4485701; ThermoFisher Scientific, Waltham, MA, USA) and PowerTrack SYBR Green Master Mix (A46109 ...
-
bioRxiv - Biophysics 2022Quote: The proteoliposomes were incubated with buffer IN (100 mM KCl, 20 mM HEPES pH 7, containing either 250 μM Calcein (Invitrogen), 100 μM Fura-2 (ThermoFischer Scientific) ...
-
bioRxiv - Cell Biology 2022Quote: Cellular glucose uptake was measured by culturing cells in glucose free DMEM for 2 hours before adding 100µM of the fluorescent glucose analogue 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl) Amino)-2-Deoxyglucose (2-NBDG; ThermoFisher, N13195). The analogue was incubated with MCF7s for 30 minutes and MSCs for 60 minutes at 37°C ...
-
bioRxiv - Cancer Biology 2023Quote: ... 3.6 x 106 HEK293T17 cells were transfected on a 6-well plate with 7 µl Lipofectamine 3000 (Thermo Fisher Scientific), the packaging plasmids psPax2 (3 µg ...
-
bioRxiv - Biophysics 2023Quote: ... cells were incubated with a dye master mix containing CellEventTM Caspase 3/7 Green Detection Reagent (ThermoFisher Scientific, 1:1000) and Zombie NIR fixable viability stain (BioLegend ...
-
bioRxiv - Genetics 2023Quote: ... Quantitative real time PCR (n = 7–8 per genotype) was performed on an ABI 7300 Sequence Detection system (Applied Biosystems) using the PowerUp SYBR Green Master Mix Kit (Applied Biosystems).
-
bioRxiv - Microbiology 2023Quote: ... approximately 5×105 Huh-7 cells were transfected with 2 µg of purified BAC DNA using Lipofectamine 3000 (Fisher Scientific) as a transfection reagent ...
-
bioRxiv - Cell Biology 2023Quote: ... Cells were passaged every 5-7 days once they reached about 70-80% confluency using TrypLE cell dissociation reagent (Gibco) and gentle scraping before being replated onto new MEFs ...
-
bioRxiv - Microbiology 2023Quote: ... Quantitative Real-Time PCR was performed using KAPA SYBR FAST One-Step kit (KAPA) on a QuantStudio 7 Flex (ThermoFisher) with the following cycling conditions ...
-
bioRxiv - Cell Biology 2023Quote: ... Cells were stained with Annexin V and propidium iodide using Annexin V apoptosis detection kit (Invitrogen Cat# 50-929-7) as per manufacturers’ instructions ...
-
bioRxiv - Cancer Biology 2023Quote: ... cells were treated with either 0.1% DMSO or 7 nM bortezomib and 30 min prior to fixation cells were treated with MitoTracker Deep Red (500 nM, Invitrogen). 16 % paraformaldehyde (EMS ...
-
bioRxiv - Microbiology 2023Quote: ... 7% of heat-inactivated fetal bovine serum (FBS) and 2% of G-418 disulfate 50mg/mL (all from ThermoFisher Scientific).