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Citations for Thermo Fisher :
1751 - 1800 of 10000+ citations for Dengue Virus Serotype 2 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: The proteins were expressed in Sf9 cells (Bac-To-Bac expression system; Invitrogen) and purified using Ni-affinity followed by Strep-Tactin affinity purification ...
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bioRxiv - Molecular Biology 2022Quote: ... Plasmids expressing fusion protein were transiently transfected into Expi293F cells (Thermo Fisher Scientific) using polyethyleneimine (Polysciences) ...
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bioRxiv - Cell Biology 2022Quote: ... These proteins were expressed in One Shot™ BL21 (DE3) competent cells (Invitrogen).
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bioRxiv - Biochemistry 2022Quote: ... the protein was overexpressed in One Shot BL21(DE3)pLysS E.coli cells (Invitrogen) in LB media at 37 °C for 8 h ...
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bioRxiv - Microbiology 2021Quote: ... Cell lysates were then pre-cleared by addition of Dynabeads Protein G (ThermoFisher) and rotated at 4°C for 3 h ...
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bioRxiv - Immunology 2020Quote: ... FreeStyle™ 293F cells for protein production were obtained from Life Technologies (#R79007) and cultured in FreeStyle™ 293 Expression Medium (Life Technologies ...
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bioRxiv - Immunology 2020Quote: ... The protein was expressed in FreeStyle 293-F cells (ThermoFisher Scientific; Cat# R79007) and purified by immuno-affinity purification using 2G12 mAb as previously described 7 ...
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bioRxiv - Microbiology 2021Quote: Cells were lysed in M-PER Mammalian Protein Extraction Reagent (Thermo Fisher Scientific). Protein lysates were run on 4%–12% Bis-Tris gels (Invitrogen) ...
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bioRxiv - Cancer Biology 2022Quote: Cell Stimulation Cocktail (plus protein transport inhibitors) (500X) (Thermo Fisher, #00-4975-93) was diluted in T cell medium (RPMI 1640 + 10%FBS + L-glutamine (2 mM ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell extracts were quantified using Pierce BCA Protein Assay Kit (Thermo Scientific; PIER23225) and 1mg was incubated with 40µl of Flag-M2 Affinity Agarose Gel (Sigma-Aldrich ...
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bioRxiv - Biophysics 2022Quote: ... These proteins were expressed in BL21 Star (DE3) Escherichia coli cells (Life Technologies). The expression and purifications were either in LB or M9 minimal media supplemented with 15NH4Cl with slight modifications as previously described (Conicella et al. ...
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bioRxiv - Systems Biology 2020Quote: ... PM proteins were extracted using a cell surface isolation kit (Thermo Fisher Scientific), whereas mitochondrial proteins were isolated using the Mitochondria Isolation Kit (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... The proteins cloned in pTYB vectors were expressed in BL21(DE3) cells (Invitrogen), grown in Terrific Broth medium at 37 °C until the OD600 reached a value of 1.5-2 ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein samples were subsequently prepared by lysing cells in RIPA buffer (Life technologies) supplemented with protease and phosphatase inhibitor cocktails (Sigma ...
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bioRxiv - Molecular Biology 2019Quote: Cas9 protein variants were expressed in BL21 star (DE3) cells (Thermo Fisher Scientific) using a previously established protocol with minor modifications (Jinek et al. ...
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bioRxiv - Neuroscience 2019Quote: ... All proteins were expressed in Human Embryonic Kidney (HEK) 293 Freestyle cells (Invitrogen) in suspension culture using serum-free media ...
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bioRxiv - Immunology 2020Quote: ... cell protein lysates were harvested using a lysis and extraction kit (ThermoFisher Scientific) supplemented with Halt Protease & Phosphatase Inhibitor Cocktail (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2021Quote: This recombinant S protein was overexpressed using the HEK 293F mammalian cells (Invitrogen) at 37°C under 5% CO2 in a Multitron-Pro shaker (Infors ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Dimeric sACE22-IgG1 proteins were expressed in ExpiCHO-S cells (Thermo Fisher Scientific) cultured in ExpiCHO Expression Medium (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2020Quote: Tissue and whole cell lysates were prepared in Protein Extraction Reagent (Thermo Fisher) supplemented with Halt Protease and Phosphatase Inhibitor Cocktail (Thermo Fisher) ...
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bioRxiv - Immunology 2021Quote: ... Cells were lysed in M-PER® Mammalian Protein Extraction Reagent (Thermo Fisher) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: Cell lysates were generated with M-PER Mammalian Protein Extraction Reagent (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: All proteins were transiently transfected in suspension HEK293F cells (Invitrogen, cat no. R79009) maintained in Freestyle medium (Life Technologies) ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were disrupted using B-PER Bacterial Protein Extraction Reagent (ThermoFisher cat#78248) containing 6 mg/ml lysozyme ...
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bioRxiv - Biochemistry 2022Quote: All recombinant proteins were produced in Rosetta2 Escherichia coli competent cells (Thermo Fisher) induced with 0.4 mM isopropyl-β-d-1-thiogalactoside (IPTG ...
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bioRxiv - Cancer Biology 2022Quote: ... B16F10 cells were transfected with labeled sgRNAs and Cas9 protein (Thermo Fisher Scientific) using CRISPRMax Cas9 transfection reagent (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... Cell lysates were pre-cleared with 30 μl protein G beads (Life Technologies), and then incubated with 2 μg isotype matched IgG control or indicated antibodies on a rocking platform overnight at 4℃ ...
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bioRxiv - Physiology 2022Quote: Protein was extracted from cells and whole kidneys using RIPA buffer (Thermo Scientific) with protease and phosphatase inhibitors (Roche) ...
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bioRxiv - Immunology 2023Quote: ... The cell-free supernatant was incubated overnight with either protein A resin (ThermoFisher) for IgG1 or LambdaFabSelect or KappSelect resin (Cytvia ...
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bioRxiv - Neuroscience 2023Quote: ... protein lysates were derived from cell cultures using RIPA buffer (#89900, Thermo Scientific) containing both protease and phosphatase inhibitors (#11836170001 ...
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bioRxiv - Immunology 2023Quote: ... RBD proteins were expressed in-house in Expi293F human cells (Thermo Fisher Scientific) by transfection of the cells with purified DNA and polyethylenimine (PEI) ...
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bioRxiv - Biophysics 2022Quote: ... was used to conduct protein expression in HEK 293-F cells (Gibco, USA) following the manufacturer’s protocol ...
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bioRxiv - Physiology 2023Quote: ... Recombinant proteins were produced in suspension culture in Freestyle 293F cells (Life Technologies) transiently transfected using FectroPro reagent (Polyplus) ...
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bioRxiv - Biochemistry 2023Quote: Sf9 cells used for expressing proteins with baculovirus system were procured from Gibco™ Sf9 cells in Sf-900™ III SFM ...
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bioRxiv - Cancer Biology 2023Quote: Proteins were obtained by cell lysis using RIPA buffer (Thermo Fisher Scientific #89901) containing Protease Inhibitor (Thermo Fisher Scientific #78438 ...
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bioRxiv - Physiology 2023Quote: ... and cells were lysed in mammalian protein extraction reagent (M-PER; Thermo Fisher) containing protease inhibitors cocktail (Roche) ...
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bioRxiv - Cell Biology 2023Quote: ... Pierce™ cell surface protein biotinylation and isolation kit (Thermo Fisher Scientific, A44390). LysoSensorTM Yellow/Blue DND160 (Invitrogen™ ...
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bioRxiv - Cell Biology 2023Quote: ... Suspension Expi293 cells used for protein purifications were grown in Expi293 media (ThermoFisher) at 37 °C/8% CO2/80% humidity in an Infors Multitron shaker.
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bioRxiv - Biochemistry 2023Quote: ... wild-type and mutant proteins were expressed in Expi293F cells (Thermo Fisher Scientific) using the same protocol.
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bioRxiv - Biochemistry 2023Quote: Proteins from human cell lines were extracted in IP lysis buffer (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: Cell or tissue samples were homogenized and proteins extracted using RIPA (Thermofisher, USA). After quantification ...
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bioRxiv - Microbiology 2023Quote: ... The proteins were expressed in SF9 or High Five™ Cells (Thermo Fisher) (BTI-TN-5B1-4 ...
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bioRxiv - Cancer Biology 2024Quote: ... Whole-cell protein lysates were resolved on 4%–12% Bis-Tris gels (Invitrogen) and transferred to nitrocellulose membranes (Bio-Rad) ...
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bioRxiv - Cancer Biology 2024Quote: Whole-cell protein extraction was completed using Pierce™ RIPA buffer (ThermoFisher 88901) supplemented with Protease Inhibitor Cocktail (Sigma P8340-5ML ...
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bioRxiv - Cancer Biology 2019Quote: Single dissociated ovarian cancer cells derived from cell lines were plated at 500 cells/ml in 2 ml serum-free DMEM-F12 (GIBCO; Invitrogen) supplemented with 5 µg/ml insulin (SAFC Biosciences ...
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bioRxiv - Biophysics 2021Quote: ... Cell nucleus were stained with DAPI (4’,6-diamidino-2-phenylindole, Invitrogen) for 10 min at room temperature ...
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bioRxiv - Biophysics 2021Quote: ... U-2 OS cells were detached using TrypLE Express enzyme (Gibco, 12604013) and plated on gelatin (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were incubated for 1 h with 2 μM EdU (Thermo Fisher) and fixed in 70% ethanol ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were stained with 4’,6diamidino-2-phenylindole (DAPI, 1:300, Invitrogen) at 37 °C for 5 min in PBTX solution ...
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bioRxiv - Molecular Biology 2020Quote: Hela CCL-2 and RPE1 (ATCC) cells were maintained in DMEM (Gibco) supplemented with 10% fetal calf serum (Gibco) ...