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Citations for Thermo Fisher :
2001 - 2050 of 10000+ citations for Dengue Virus Serotype 2 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... and stained for SARS-CoV-2 N protein using Biotin-labelled-CR3009 antibody produced in-house together with a Streptavidin-Alexa488 (S32354, Invitrogen) and cellular DNA using DAPI (10236276001 ...
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bioRxiv - Biophysics 2023Quote: ... The final protein concentration used for all measurements was ~2 mg/ml as calculated using A280 measurements on a NanoDrop (ThermoFisher) and adjusted by dilution with buffer B at pH 9.4 ...
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bioRxiv - Cancer Biology 2023Quote: The sonicated samples (approximately 5 µg chromatin/sample) were cleared for 2 hours using non-coupled magnetic beads (Protein G Dynabeads, Invitrogen), then further mixed with 5 µL antibody (anti-H3K27me3 ...
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bioRxiv - Genetics 2023Quote: ... Protein was separated on a 4–12% Bis-Tris gel and transferred using iBlot 2 transfer apparatus (Thermo Fisher Scientific). A 5% milk solution in TBST was used for blocking for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... The supernatant was incubated o/n at with rotation at 4°C with 2 µg IP antibody and for 1 h with Dynabeads Protein G (Invitrogen). Alternatively ...
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bioRxiv - Molecular Biology 2023Quote: Protein (2 µg total lysate, 5 µg soluble lysate) was loaded onto 4-12% Bis-Tris NuPAGE minigels (Invitrogen, NP0322BOX). 10 µL of precision plus protein kaleidoscope prestained standard (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... or cytosolic fractions were incubated with 2 µL of primary antibody and 100 µL of Dynabeads™ Protein G (Invitrogen) overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Endogenous SART1 was depleted from CSF extracts by 2 rounds of incubation with 60% (vol/vol) Dynabeads Protein A (Invitrogen) coupled with Xenopus SART1 antibodies.
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bioRxiv - Cancer Biology 2024Quote: ... 200–500 μg of nuclear lysates were incubated with 2 μg of specific antibodies and 50 μg of Dynabeads Protein G (Invitrogen). Following an overnight incubation on a rotator at 4 °C ...
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bioRxiv - Molecular Biology 2024Quote: The transfection of a mixture of specific siRNA against c-Myc protein (exon 2 and exon 3, IDs s9129 and s9130, respectively; Ambion) and DOT1L protein (IDs s39011 ...
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bioRxiv - Genetics 2021Quote: ... Cells were dissociated to single cells with TrypLE Express and fixed in 2% formaldehyde in DPBS (Thermo Fisher) for 10 min ...
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bioRxiv - Microbiology 2020Quote: Caco-2 cells and MDCK cells were maintained in T25 tissue culture flasks (Falcon) with DMEM medium (Gibco) supplemented with 10% fetal bovine serum at 37°C in a CO2 incubator.
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bioRxiv - Microbiology 2021Quote: HeLa cells (ATCC CCL-2) and HEK293 cells (ATCC CRL-1573) were maintained in DMEM high glucose (Gibco) containing 10% heat-inactivated fetal bovine serum (Gibco ...
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bioRxiv - Systems Biology 2022Quote: ... HEK293 cells were prepared as 2 × 105 cells/mL in DMEM (Dulbecco s Modified Eagle Media, Life Technologies) + 10% FBS (Foetal Bovine Serum ...
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bioRxiv - Systems Biology 2022Quote: ... Infected cells grew for 2 days and then the cells were selected with blasticidin (10 mg/mL, Gibco). Infection and selection efficiency were monitored each day using flow cytometry to measure mCherry (Biorad ZE5) ...
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bioRxiv - Molecular Biology 2021Quote: ... and diluted to 1-2 million cells/mL using a Countess™ Automated cell counter (Invitrogen; Carlsbad, CA). On each day of experiments ...
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bioRxiv - Molecular Biology 2023Quote: HeLa cells (ATCC #CCL-2) and MCF7 cells (gift from Matt Steensma) were maintained in DMEM (Gibco, 11965092) supplemented with 10% FBS (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... Cell lysis and cDNA generation was performed using the 2-Step Fast-Cells-to-CT- Kit (Invitrogen, ThermoFisher) according to their protocols ...
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bioRxiv - Cell Biology 2023Quote: ... Cell lysis and cDNA generation was performed using the 2-Step Fast-Cells-to-CT- Kit (Invitrogen, ThermoFisher) according to their protocols ...
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bioRxiv - Microbiology 2023Quote: ... with 100 µL cell suspension at the density of 2×105 cells mL-1 in Dulbecco’s modified Eagle’s medium (DMEM) (GIBCO) supplemented with 10% (v/v ...
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bioRxiv - Bioengineering 2023Quote: Embryonic multipotent mesenchymal progenitor cells (10T1/2 cells) (ATCC) were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplemented with 5% v/v Fetal Calf Serum (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were resuspended at 2 x 106 cells/ml in assay medium containing DMEM/F12 (1:1, Gibco) supplemented with 5% horse serum (Invitrogen) ...
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bioRxiv - Immunology 2023Quote: ... RD114 cells were seeded at 2 × 105 cells/well in a 6-well plate with DMEM (Gibco, USA) supplemented with 10% FCS (Sigma-Aldrich ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Cells (2×107 cells/mL) in assay medium consisting of phenol red-free RPMI-1640 (Thermo Fisher Scientific) supplemented with 0.1% fatty acid-free bovine serum albumin (Sigma ...
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bioRxiv - Developmental Biology 2023Quote: ... a blastomere was isolated and treated with 2 μL Cells-to-cDNA™ II Cell Lysis Buffer (Invitrogen) at 75°C for 15 min ...
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bioRxiv - Biophysics 2023Quote: In vitro cytotoxicity of the complexes was evaluated using U-2 OS cells and an LDH assay kit.42 Cells were counted with a Countess II Automated Cell Counter (ThermoFisher Scientific, USA) and seeded in 96-well plates at a density of 10,000 cells per well in 100 μL of full DMEM ...
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bioRxiv - Cancer Biology 2023Quote: LN-229 cells were seeded at 2×10^5 cells/well into in 6-well Nunc™ Cell-Culture Treated Multidishes (ThermoFisher, #140675) and incubated overnight in reduced serum media at 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... All iPSCs were generated by Sendai virus reprogramming and clonal expansion using the CytoTune – iPSC 2.0 kit (ThermoFisher) to deliver the reprogramming factors ...
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bioRxiv - Cell Biology 2019Quote: Cholesterol concentration in purified virus was determined using Amplex™ Red Cholesterol Assay Kit (Molecular Probes, Thermo Fisher) according to manufacturer’s instruction ...
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bioRxiv - Cell Biology 2019Quote: Cholesterol concentration in purified virus was determined using Amplex™ Red Cholesterol Assay Kit (Molecular Probes, Thermo Fisher) according to manufacturer’s instruction ...
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bioRxiv - Microbiology 2020Quote: ... Viral RNA was quantified via qRT-PCR using TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher Scientific) on a QuantStudio 3 (Applied Biosystems) ...
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bioRxiv - Microbiology 2022Quote: ... Total RNA extractions of 80 μL of virus stocks lyzed with 240 μL of TRIzol LS Reagent (Invitrogen) were performed in triplicate with the Direct-zol RNA MiniPrep kit (Zymo Research) ...
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bioRxiv - Microbiology 2022Quote: ... All the procedures follow the manufacturer’s instructions of the Applied Biosystems TaqMan Fast Virus one-step mastermix (ThermoFisher). Briefly ...
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bioRxiv - Cancer Biology 2022Quote: ... One microgram of RNA was retrotranscribed to complementary DNA using moloney murine leukemia virus reverse transcriptase (Thermo Fisher) and random hexamer primers (Roche) ...
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bioRxiv - Pathology 2021Quote: ... Total RNA from virus supernatants was extracted using the PureLink™ Viral RNA/DNA Mini Kit (Thermo Fisher) according to the manufacturer’s protocol and then converted to cDNA by SuperScript® III Reverse Transcriptase (Invitrogen) ...
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bioRxiv - Microbiology 2021Quote: ... we exchanged the medium in the filtered virus collections three times with Opti-MEM I (1X) + GlutaMAX (Gibco) using an Amicon Ultra-15 column (100 kDa cutoff) ...
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bioRxiv - Microbiology 2022Quote: ... quantitative polymerase chain reaction (qPCR) was performed using TaqMan Fast virus 1 Step PCR Master Mix (Applied Biosystems); a standard curve was drawn with 2019_nCOV_N Positive control (IDT) ...
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bioRxiv - Immunology 2022Quote: ... Reverse-transcription of isolated RNA was performed using a Moloney murine leukemia virus-reverse transcriptase kit (Life Technologies) and oligo (dT ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Quantitative real-time PCR was performed using a TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher Scientific), 2019-nCoV RUO Kit (Integrated DNA Technologies ...
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bioRxiv - Genetics 2021Quote: ... Samples KAB_3 and KAB_7 were reprogrammed using Sendai virus (CytoTune™-iPS 2.0 Sendai Reprogramming Kit, ThermoFisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2020Quote: Concentrated virus particles were treated with RNase A before extraction of RNA using Trizol LS (Ambion, Life Technologies). The aqueous fraction of the Trizol lysate was precipitated with ethanol and was resuspended in 10 mM Tris pH 8.0 before purification using the QIAamp viral RNA mini kit (QIAGEN ...
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bioRxiv - Evolutionary Biology 2020Quote: Concentrated virus particles were treated with RNase A before extraction of RNA using Trizol LS (Ambion, Life Technologies). The aqueous fraction of the Trizol lysate was precipitated with ethanol and was resuspended in 10 mM Tris pH 8.0 before purification using the QIAamp viral RNA mini kit (QIAGEN ...
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bioRxiv - Microbiology 2021Quote: ... Transmissible gastroenteritis virus [TGEV] and Swine deltacoronavirus [SDCoV] (VetMAX™ PEDV/TGEV/SDCoV, Thermo Fisher Scientific, MA, USA) and Senecavirus A [SVA] (EXOone Seneca Virus Valley ...
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bioRxiv - Immunology 2020Quote: ... Reverse transcription of isolated RNA was performed using a Moloney murine leukemia virus-reverse transcriptase kit (Life Technologies) and oligo(dT ...
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bioRxiv - Synthetic Biology 2022Quote: ... undiluted SINV-containing supernatants were combined with the TaqMan™ Fast Virus 1-Step Master Mix (ThermoFisher, #4444434) and the appropriate primer-probe sets in technical triplicates ...
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bioRxiv - Microbiology 2022Quote: ... Premixes were prepared for each amplification reaction using TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher Scientific), according to the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2022Quote: ... C and S genes of the Hepatitis B virus (accession number: KY00323035) was ordered from GeneArt (ThermoFisher Scientific) and is available from Addgene (Addgene # 179155).
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bioRxiv - Neuroscience 2023Quote: ... C-Myc) introduced by Sendai virus according to the manufacturer’s instructions (Cytotune iPS 2.0 Sendai reprogramming kit, ThermoFisher) or by using an episomal method ...
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bioRxiv - Cell Biology 2023Quote: ... After a brief culture to expand the erythroblast population a Sendai virus based approach (CytoTune 2.0 Kit, Invitrogen) was used to express the Yamanaka factors without any genomic integration (27) ...
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bioRxiv - Biochemistry 2023Quote: Bacmid preparation and virus production were performed according to the Bac-to-Bac baculovirus system manual (Gibco, Invitrogen). Spodoptera frugiperda Sf9 cells at a density of 3 × 106 cells/ml were co-infected with baculoviruses encoding receptor and Gi trimer at the ratio of 1:1 ...