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Citations for Thermo Fisher :
1601 - 1650 of 10000+ citations for Dengue Virus Serotype 2 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... subcellular protein fractionation was performed as described in the Subcellular protein fractionation kit for cultured cells (Thermo Scientific; Cat. No. 78840). Fractions were stored in −80°C per manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: Protein concentration in the cell extract was measured using a Pierce BCA protein assay kit (product 23227; Thermo Scientific, Rockford, IL).
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bioRxiv - Animal Behavior and Cognition 2021Quote: Total proteins were isolated from cells and tissues using the Nuclear and Cytoplasmic Protein Extraction Kit (Thermo Fisher Scientific, Waltham, USA). Approximately 40 μg of protein was separated on 12% SDS-polyacrylamide gel (SDS-PAGE ...
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bioRxiv - Neuroscience 2021Quote: ... Total protein amounts of the OM cell lysates were quantified with BCA assay (Pierce™ BCA Protein Assay Kit, Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... and the protein concentration of the cell lysates was then determined using Pierce™ BCA Protein Assay Kit from ThermoFisher (#23225). After 100 mM DTT was added ...
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bioRxiv - Molecular Biology 2022Quote: ... The protein contents of total cell lysates were determined using a BCA protein assay kit (Thermo Scientific Pierce, Rockford, IL, USA). The equal amounts of protein were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) ...
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bioRxiv - Genomics 2023Quote: ... Eluted proteins and 50 μg of protein from whole cell lysate were resolved in 4-15% NuPAGE gradient gel (ThermoFisher, NP0335) using MOPS running buffer (ThermoFisher ...
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bioRxiv - Immunology 2023Quote: Total proteins from intestinal epithelial cells or intestinal organoids were extracted with M-PER Mammalian Protein Extraction Reagent (Thermo Fisher Scientific) with proteinase inhibitor (Roche ...
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bioRxiv - Cell Biology 2023Quote: ... For normalization total protein concentration was measured on an aliquot of the cell lysate with the Pierce BCA protein kit (Thermo Fisher) following the manufacturer’s instructions ...
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Volumetric Compression Shifts Rho GTPase Balance and Induces Mechanobiological Cell State TransitionbioRxiv - Biophysics 2023Quote: ... A small aliquot of cell lysate was later thawed and used for total protein quantification following the “BCA Protein Assay Kit” manual (Thermo Scientific). The same quantity (60 μL ...
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bioRxiv - Microbiology 2021Quote: ... HEK 293 cells and CaCo-2 cells were cultured in DMEM/F-12 and DMEM (Gibco), respectively ...
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bioRxiv - Bioengineering 2021Quote: ... cells were seeded at 2 × 105 cells/well in round-bottom 96-well plates (Fisher Scientific) in IMDM with 10% FBS and 2% penicillin/streptomycin (Life Technologies) ...
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Activation of endoplasmic reticulum stress via clustering of the inner nuclear membrane protein SUN2bioRxiv - Cell Biology 2022Quote: ... U-2 OS cells (ATCC® HTB-96™) and HEK293FT cells (Thermo Fisher Scientific, R70007) were grown in DMEM containing 10% fetal bovine serum (FBS) ...
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bioRxiv - Neuroscience 2022Quote: ... HEK293 cells were seeded at 2 ×105 cells per well in six well plates (Thermo Fisher) in DMEM/10% FBS ...
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bioRxiv - Immunology 2022Quote: ... The cells released cells were subsequently cultured under hypoxic conditions (2% O2) in α-MEM (ThermoFisher) supplemented with 20% FBS (Gibco) ...
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bioRxiv - Cell Biology 2023Quote: For Live Cell Microscopy – Cells were grown on Lab-Tek 2-well chambered cover glass (Nunc) under normal growth condition and imaged on OLYMPUS FV3000 Laser scanning microscope at 37°C and 10 % CO2 ...
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bioRxiv - Biochemistry 2023Quote: ... Sf9 cells were transfected with Bacmid DNA (1-2 µg/106 cells) using Cellfectin II (Invitrogen). The supernatant containing P1 baculoviruses was harvested after 5 to 7 days ...
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bioRxiv - Physiology 2024Quote: ... These cells were generated by transfecting HEK293 cells using Lipofectamine 2000 (Invitrogen; 2 μl per well) with D1ER cDNA (0.5 μg/well ...
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bioRxiv - Immunology 2023Quote: Unmodified K562 cells and CD54−/−CD58−/− K562 cells were labeled with 2 uM CellTrace Violet (ThermoFisher) and co-cultured with purified NK cells at varying effector:target ratios ...
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bioRxiv - Molecular Biology 2021Quote: ... the input aliquot (100 µl) was mixed with equal volume of 2× protein sample buffer (2× NuPAGE LDS buffer [Life Technologies], 100 mM DTT, 4% [w/v] SDS) and the IP with 100μl 1 x protein sample buffer followed by incubation for 10 min at 75 °C ...
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bioRxiv - Microbiology 2019Quote: ... were transfected into BV-2 cells or NA cells (106 cells/well) in 12-well plates using Lipofectamine 3000 (Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2019Quote: Single dissociated ovarian cancer cells derived from cell lines were plated at 500 cells/ml in 2 ml serum-free DMEM-F12 (GIBCO; Invitrogen) supplemented with 5 µg/ml insulin (SAFC Biosciences ...
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bioRxiv - Cancer Biology 2022Quote: ... T cells were then resuspended at 2 × 106 cells/ml in PBS and stained with Cell Trace Violet (1:2000, Invitrogen) for 20 minutes at 37°C ...
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bioRxiv - Molecular Biology 2019Quote: Relative transcript abundance of HarmGr9 in control and Gr9 silenced insects was determined by quantitative Real-Time PCR (qRT-PCR) using 7900HT Fast Real-Time PCR System (Applied Biosystems, Foster, CA, USA) and GoTaq® qPCR Master Mix (Promega ...
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bioRxiv - Neuroscience 2021Quote: ... The wall was coated with Insect-a-Slip (Bioquip Products, Inc., cat# 2871C) and the ceiling was coated with Surfasil Siliconizing Fluid (ThermoFisher Scientific, cat# TS-42800), to prevent flies from climbing as described previously (Asahina et al. ...
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bioRxiv - Neuroscience 2020Quote: ... Each circular arena measured 16 mm diameter x 12 mm high and was coated with Insect-a-Slip (Bioquip Products, Rancho Dominguez, CA) on the walls and SurfaSil Siliconizing Fluid (Thermo Fisher Scientific, Waltham, MA) on the clear acrylic top plate to confine the flies to the bottom plate ...
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bioRxiv - Cancer Biology 2021Quote: ... were cultured in EBM-2 Basal Medium supplemented with EGM-2 MV Microvascular Endothelial Cell Growth Medium and 1% v/v Pen-Strep (ThermoFisher) according to manufacturer’s instructions.
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bioRxiv - Neuroscience 2020Quote: Staining the cells with the mitochondrial selective dye Rhod-2 AM (rhodamine-2 acetoxymethyl ester; Thermo Fisher Scientific, Darmstadt, Germany), allows for specific evaluation of mitochondrial calcium accumulation ...
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bioRxiv - Biochemistry 2021Quote: ... at a concentration of 2 × 105 cells ml−1 (2 ml per dish hereafter) in DMEM (Nissui Pharmaceutical) supplemented with 10% FBS (Gibco), glutamine ...
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bioRxiv - Biophysics 2022Quote: ... at a concentration of 2×105 cells/ml (2 mL per well hereafter) in DMEM (Nissui Pharmaceutical) supplemented with 10% (v/v) FBS (Gibco), glutamine ...
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bioRxiv - Immunology 2019Quote: Mouse spleens were forced through a 70 μm mesh into FACS buffer (PBS containing 2 % heat-inactivated FBS and 2 mM EDTA) and red blood cells were lysed in ACK lysing buffer (Gibco) for 3 min ...
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bioRxiv - Neuroscience 2023Quote: ... the pellet was washed with HBSS and then resuspended in 10 mL magnetic-activated cell sorting (MACS) buffer (Hibernate-A with 2 mM sodium pyruvate, 2% B27, 0.5% BSA; ThermoFisher Scientific), 2 mM EDTA (Sigma-Aldrich ...
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bioRxiv - Microbiology 2019Quote: ... cells were washed with PBS and fractionated using a subcellular protein fractionation kit for cultured cells (Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: Proteins were extracted from shβ7 and pTer1 cells using Cell Disruption Buffer (miRVana™ Paris™ kit, Thermofisher) and were quantified with microBCA protein Assay kit (Thermofisher ...
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bioRxiv - Bioengineering 2020Quote: ... Intensity quantification was performed using ImageJ software and the obtained values were corrected by culture media background (without having been in contact with cells) and total protein content measured using the Pierce™ BCA Protein Assay Kit (Thermo Scientific). Values were further normalized by internal negative and positive controls from the antibody assay ...
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bioRxiv - Genetics 2022Quote: Total protein was isolated from kidneys or cells using lysis buffer made from mixing T-PER tissue protein extraction reagent (Invitrogen, catalog# 78510) with a protease phosphatase inhibitor tablet (Fisher ...
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bioRxiv - Cell Biology 2019Quote: ... Protein concentrations of the tissue homogenates or whole-cell extracts were determined using the Pierce BCA protein assay kit (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2021Quote: ... 5 mM MgCl2) for cell membrane binding proteins (LPL, Caveolin 1) and quantified with Pierce™ BCA Protein Assay Kit (Buffer (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... Mitochondria enriched fractions were processed similar to whole cell samples except they were normalized via protein concentration as determined by the Pierce™ BCA Protein Assay kit (Thermo Scientific). In Brief ...
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bioRxiv - Neuroscience 2022Quote: ... Plates were read using an MSD SECTOR Imager 2400 and resulting peptide concentrations were normalized to total protein in the cell lysate per well measured using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). Data for each shRNA knockdown were additionally normalized to the average of control conditions for each parameter measured.
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bioRxiv - Physiology 2023Quote: ... BAL cells were counted using a LUNA-II automated cell counter (Logos Biosystems) and BAL supernatant total protein was measured using a Pierce total protein assay (Thermo Scientific, #23225).
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bioRxiv - Immunology 2023Quote: Supernatants were collected from cell cultures and protein extracts of lung tissue were obtained using T-PER Tissue Protein Extraction Reagent (ThermoFisher, Cat# 78510). The levels of mouse IL-5 and IL-13 were measured with DuoSet ELISA kits (DY405 and DY413 respectively ...
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bioRxiv - Neuroscience 2023Quote: ... was recombinantly expressed as an N-terminal Fc-fusion protein (Fc-hTfR1) in Expi293 suspension cells and purified by gravity flow with PierceTM Protein A Agarose (Thermo Fisher Scientific). HFCII probes were loaded with Fc-hTfR1 by thresholding to a 1 nm shift ...
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bioRxiv - Cell Biology 2024Quote: ... Following treatment cells were then transduced with Premo™ Autophagy tandem sensor Red Fluorescent Protein-Green Fluorescent Protein (RFP-GFP)-LC3B (Life Technologies, Invitrogen) and chloroquine was used as control ...
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bioRxiv - Genetics 2021Quote: ... protein transfer to a nitrocellulose membrane was completed using the iBlot 2 Dry Blotting System (Thermo Fisher Scientific Scientific). The membrane was then blocked for one hour at room temperature using a 5% milk solution in 1× TBST ...
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bioRxiv - Molecular Biology 2020Quote: ... were resolved on precast NuPAGE 4-12% Bis-Tris midi 12+2-well protein gels (cat. no. WG1401BOX, Invitrogen) at 200 V for 40 min in NuPAGE 2-(N-morpholino)ethanesulfonic acid (MES ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by the transferring of resolved proteins onto PVDF membranes via an iBlot 2 Dry Blotting System (ThermoFisher Scientific). Following blocking for 60 min with 1% BSA in TBST (10 mM Tris-HCl (pH7.4) ...
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bioRxiv - Neuroscience 2019Quote: ... An equal amount of protein per sample was loaded into 2-12% gradient NuPage Bis-Tris gels (Thermo Scientific) and protein was separated by electrophoresis ...
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bioRxiv - Biochemistry 2021Quote: SARS-CoV-2 S protein Omicron genes were synthesized and inserted into pcDNA3.1 (GeneArt Gene Synthesis, Thermo Fisher Scientific). Pseudotyped retroviral particles were produced in HEK293T cells as described previously (32) ...
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bioRxiv - Cancer Biology 2020Quote: ... Electrophoresis was performed and proteins were dry transferred to a nitrocellulose membrane using an iBlot 2 system (ThermoFisher Scientific). The blots were probed with anti-phospho-AKT (Ser473 ...