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Citations for Thermo Fisher :
1701 - 1750 of 10000+ citations for Dengue Virus Serotype 2 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Real-time RT-PCR analysis was performed using Fast Virus 1-Step Master Mix (Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... iPSC lines were generated using the Sendai virus (CytoTuneTM-iPS 2.0 Sendai Reprogramming Kit, Thermo Fisher Scientific) carrying the Yamanaka reprogramming factors OCT3/4 ...
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bioRxiv - Neuroscience 2023Quote: Expanded PBMCs were transduced Sendai virus reprogramming vector – according to CytoTune® 2.0 Sendai (Thermo Fisher Scientific). Before cells infection ...
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bioRxiv - Neuroscience 2023Quote: ... medium containing virus was processed by centrifugation (Sorvall WX-90 Ultracentrifuge and SureSpin 630 rotor; ThermoFisher Scientific), using a sorbitol cushion (20% sorbitol ...
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bioRxiv - Microbiology 2023Quote: ... Presence of each virus strain in the supernatant was determined by PCR using Taq polymerase (Thermo Scientific) and 20 μL reactions containing 1 μL of virus supernatant ...
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bioRxiv - Genetics 2023Quote: ... The PBMCs were reprogrammed by using Sendai virus (CytoTuneTM-iPS 2.0 Sendai Reprogramming kit, Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... Isolated ZIKV RNA was titrated by qRT-PCR using TaqMan Fast virus 1-step master mix (ThermoFisher) and a LightCycler 480 or LC96 instrument (Roche) ...
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bioRxiv - Microbiology 2023Quote: ... The following primary antibodies were used: influenza A virus nucleoprotein (NP, PA5-32242, 1:3000, ThermoFisher Scientific), XPO1 (46249S ...
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bioRxiv - Molecular Biology 2023Quote: ... purified gRNA from benzonase-treated virus material was treated with DNaseI (Thermo Fisher Scientific, Waltham, MA, USA), and analyzed using an Agilent 2100 bioanalyzer (Agilent Technologies ...
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bioRxiv - Bioengineering 2023Quote: ... reverse transcription was performed with random hexamers and Moloney murine leukaemia virus (M-MLV) reverse transcriptase (Invitrogen). Synthesized RNA was used as a standard (BEI) ...
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bioRxiv - Cell Biology 2023Quote: ... from a cDNA library of HCT116 which was prepared using Moloney murine leukemia virus reverse transcriptase (Invitrogen), as described previously (Ninagawa et al. ...
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bioRxiv - Biochemistry 2023Quote: ... virus was harvested by collecting supernatant and filtering it through 0.45µm PES sterile filter (Thermo Fisher, 2954545). Virus was concentrated by ultracentrifugation with a 20% sucrose cushion ...
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bioRxiv - Microbiology 2024Quote: ... half of the virus supernatant after the concentration step was digested with amplification grade DNAse I (Invitrogen/Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... and the virus was incubated with 25 μg of Alexa Fluor 488 NHS Ester (succinimidyl ester; Invitrogen) for 1 hour at 25°C ...
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bioRxiv - Microbiology 2024Quote: Genomic DNA was extracted from 1010 virus particles using the PureLink TM Genomic DNA mini kit (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... protein concentrations were determined by Bradford Protein Assay protein using Coomassie protein assay reagent (Thermo Fisher Scientific-Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... LX-2 cells were seeded at 200,000 cells/mL on chamber slides (Nunc Lab-Tek; Thermo Fisher), grown overnight ...
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bioRxiv - Cell Biology 2021Quote: ... LX-2 cells were seeded at 200,000 cells/mL on chamber slides (Nunc Lab-Tek; Thermo Fisher), grown overnight ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were washed directly on cell culture plates with in 2 mL DPBS (Gibco/ThermoFisher, Paisley, UK) and 1 mL of resuspension buffer (10 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were washed directly on cell culture plates with in 2 mL DPBS (Gibco/ThermoFisher, Paisley, UK) and 1 mL of resuspension buffer (10 mM Tris-HCl ...
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bioRxiv - Immunology 2022Quote: HeLa-CIITA cells were incubated in 6-well plates using 2-4.105 cells/well using OPTIMEM (Gibco) complemented with 10% FBS ...
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bioRxiv - Microbiology 2020Quote: ... Non-adherent cells were washed away after 2 h and the remaining cells incubated in RPMI (Gibco) supplemented with 10 % heat-inactivated pooled human serum (Sigma ...
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bioRxiv - Immunology 2020Quote: Proliferation of purified CD4+ T cells was assessed by labeling cells with 2 μM CellTrace Violet (Invitrogen) or 0.5 µM CFSE (Sigma-Aldrich ...
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bioRxiv - Immunology 2020Quote: ... Purified CD4+ T cells were counted and labelled with 2 μM Cell Tracker Green CMFDA dye (ThermoFisher) for 20 minutes at 37°C ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell suspensions were centrifuged and cell pellets were resuspended in 2 mL ACK lysis buffer (Gibco, A1049201). After five minutes ...
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bioRxiv - Immunology 2021Quote: ... cells were cultured at 2×106 cells/plate in DMEM/F12 GlutaMAX™(GIBCO™, #31331-028) medium supplemented with 10% FBS (GIBCO™ ...
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bioRxiv - Microbiology 2023Quote: HeLa cells (Human epithelial cells, ATCC CCL-2) were maintained in Dulbecco’s modified Eagle’s medium (DMEM, Gibco), supplemented with heat-inactivated fetal calf serum (10%) ...
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bioRxiv - Neuroscience 2022Quote: Cells were loaded with the cell-permeable (AM) version of the Ca2+ indicator fura-2 (Molecular Probes) at room temperature in L-15 medium ...
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bioRxiv - Cancer Biology 2023Quote: ... cells (2×103 cells/well) were seeded in 8-well chamber slides (Nunc Lab-Tek, Thermo Fisher) and cultured overnight in the growth medium for staining ...
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bioRxiv - Immunology 2023Quote: Proliferation of stimulated CD4+ T cells was assessed by labeling cells with 2 μM CellTrace Violet (Invitrogen) prior to culture ...
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bioRxiv - Biophysics 2023Quote: The cells were incubated with nonfluorescent cell-permeant 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA, 5μM, ThermoFisher Scientific, UK) probe for 30 min prior to ES ...
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bioRxiv - Cancer Biology 2023Quote: ... cells (2×103 cells/well) were seeded in 8-well chamber slides (Nunc Lab-Tek, Thermo Fisher) and cultured overnight in the growth medium for staining ...
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bioRxiv - Systems Biology 2024Quote: Human osteosarcoma epithelial cells (U-2 OS cell line) were cultivated in DMEM high glucose medium (Gibco), supplemented with 2 mM L-glutamine ...
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bioRxiv - Neuroscience 2021Quote: ... cells were lysed in B-PER bacterial protein extraction reagent (Thermo Scientific, MA) containing 2500 U of DNAseI (Sigma) ...
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bioRxiv - Molecular Biology 2020Quote: ... Cell protein lysates were mixed with NuPAGE LDS Sample Buffer (4X) (NP0007; Invitrogen) and run on polyacrylamide gels ...
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bioRxiv - Cell Biology 2019Quote: ... cells were transfected with siRNA control or siRNA against specific proteins (Life Technologies). DharmaFECT transfection reagent (0.1 μL per well ...
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bioRxiv - Immunology 2019Quote: ... Total cell lysates were pre-cleared on protein A or G sepharose (Invitrogen) for 1h at 4°C ...
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bioRxiv - Cancer Biology 2019Quote: Cells were lysed directly in M-PER Mammalian Protein Extraction Reagent (ThermoFisher Scientific), separated on a NuPAGE 4-12% Tris-Glycine Gel (ThermoFisher Scientific) ...
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bioRxiv - Genomics 2019Quote: Total RNA and protein were extracted from whole cells using TRIzolRT reagent (Ambion) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... All proteins were expressed in Escherichia coli one-shoot BL21star (DE3) cells (Invitrogen), as previously described (Selyutina et al. ...
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bioRxiv - Bioengineering 2019Quote: ... cells were washed once and stained with biotiny-lated Protein L (Thermo Fisher). After a subsequent wash ...
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bioRxiv - Biochemistry 2021Quote: ... Cell fractionation was carried out using the Subcellular Protein Fractionation Kit (ThermoFisher Scientific) and as described by the manufacturer ...
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bioRxiv - Biochemistry 2021Quote: ... cells were lysed in B-PER bacterial protein extraction reagent (Thermo Fisher Scientific), supplemented with TURBO DNase I (5 U/mL ...
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bioRxiv - Neuroscience 2021Quote: Cells were lysed with M-PER Mammalian Protein Extraction Reagent (Thermo Scientific, 78501) supplemented with protease inhibitor cocktail (Roche ...
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bioRxiv - Microbiology 2020Quote: ... DROSHA knockout cells were transfected with the TrueCut™ Cas9 Protein v2 (Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: Cells were lysed in M-PER® mammalian protein extraction reagent (Thermo Scientific) containing protease and phosphatase inhibitors ...
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bioRxiv - Cell Biology 2021Quote: ... the Subcellular Protein Fractionation Kit for Cultured Cells (Cat. n°: 78840, Thermo Fisher) was used according to manufacturer instructions ...
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bioRxiv - Cell Biology 2021Quote: Protein was extracted from cell lysates using RIPA buffer (Thermofisher Scientific, USA, 89900) containing Sigmafast EDTA free protease inhibitor cocktail (Sigma ...
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bioRxiv - Cancer Biology 2022Quote: ... Total cell lysates were resolved in 4-12% Bis-Tris Protein Gels (Invitrogen) and transferred in for 1.5 h (80 V ...
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bioRxiv - Cell Biology 2022Quote: ... The protein in whole-cell lysates were separated by using BlotTM Gel (Invitrogen) and transferred to nitrocellulose membranes.