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Citations for Thermo Fisher :
1901 - 1950 of 10000+ citations for Dengue Virus Serotype 2 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: McCoy’s 5A medium for U-2 OS cells (Gibco, cat. No. 16600082)
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bioRxiv - Cancer Biology 2023Quote: ... Capan-2 cells were cultured in McCoy’s 5A Medium (Gibco, Madrid, Spain) supplemented with 10 % fetal bovine serum ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were pulsed with 10μM EdU (5-ethynyl-2-deoxyuridine, Invitrogen A10044) for 30 min at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... cells were harvested and re-suspended in 2 ml DPBS (Thermo Scientific), supplemented with 10% FCS ...
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bioRxiv - Microbiology 2023Quote: ... Cells were imaged on an Apreo 2 Scanning Electron Microscope (Thermo Fisher) at 5,000x ...
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bioRxiv - Cancer Biology 2024Quote: ... cells are grown in media containing 2 μg/ml doxycycline (Thermo Fisher) and 250 nM Shield1 (Takara Bio ...
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bioRxiv - Molecular Biology 2023Quote: ... 107 cells were fixed with 2 mM disuccinimidyl glutarate (DSG; Thermo Fisher) for 50 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... cells were treated with 2 µg/ml puromycin (Invitrogen, cat. #A11138-03) for 72 h and then tested for DLG1 protein depletion by western blot analysis ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were treated with 2 μg/mL doxycycline (Thermo Fisher Scientific, BP26531) to induce expression of GFP-KIF18A and 5 pmol KIF18A siRNA to deplete endogenous KIF18A ...
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bioRxiv - Cell Biology 2023Quote: Total RNA was isolated from 2×106 cells with Trizol (Life Technologies) according to manufacturer’s instructions ...
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Self-organised pattern formation in the developing neural tube by a temporal relay of BMP signallingbioRxiv - Developmental Biology 2023Quote: Cells were passaged every 2-3 days by incubation with Accutase (Gibco) for 2-3 min at 37°C ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: The cells were loaded with 1.5 μM Fura-2 AM (Invitrogen, #F1221) and 20% BSA in the running HBSS buffer for 45 min ...
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bioRxiv - Neuroscience 2024Quote: ... and subsequently for 2 days in endothelial cell medium (EC; Life Technologies) supplemented with 2% B27 (Life Technologies) ...
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bioRxiv - Molecular Biology 2023Quote: ... Frozen cell pellets from 2 L of infected High Five (Thermo Scientific) insect cell culture were resuspended in 50 mM Tris-HCl pH 8.5 ...
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bioRxiv - Cancer Biology 2023Quote: ... Transduced cells were selected using puromycin (2 µg/ml, Thermo Fisher Scientific). For the generation of CRISPR lines ...
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bioRxiv - Cell Biology 2024Quote: D.Mel-2 cells were maintained in Schneider’s Drosophila medium (Thermo Fisher Scientific) with 10% FBS and antibiotics at 24°C ...
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bioRxiv - Cell Biology 2024Quote: ... cells were treated with 2 µg/ml puromycin (Invitrogen, cat. #A11138-03) for 72 h ...
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bioRxiv - Immunology 2024Quote: 2×106 THP-1 cells were centrifuged and washed in PBS (Gibco). They were then transfected by nucleofection with 3 μg of plasmid DNA in 100 μl of Cell Line Nucleofector Solution V (Lonza ...
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bioRxiv - Microbiology 2024Quote: ... HeLa ATCC CCL-2 cells were cultured in DMEM (41965-039, Gibco) supplemented with 10% FBS ...
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bioRxiv - Cancer Biology 2024Quote: ... Viability and cell count were assessed on Countess 2 (Invitrogen, Carlsbad, CA). Equilibrium to targeted cell recovery of 6,000 cells along with 10x Gel Beads and reverse transcription reagents were loaded to Chromium Single Cell Chip G (PN-1000120 ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were then blocked in PBST with 2% horse serum (Gibco) for 1 hour at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... coli One Shot™ ccdB Survival™ 2 T1R Competent Cells (ThermoFisher). Single colonies were cultured for plasmid isolation and the cloned sequence was confirmed by DNA sequencing.
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bioRxiv - Plant Biology 2019Quote: ... free amino acids from leaf or insect samples were extracted with vortex agitation for 30 sec at room temperature in 1 mL acetonitrile 25 % (Fisher Scientific; Hampton, New Hampshire, USA) in hydrochloric acid 0.01 N (1:3 ...
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bioRxiv - Cell Biology 2019Quote: ... The following dilutions were used for secondary antibodies and cell dyes for staining: goat anti-rabbit Alexa Fluor 488 F(ab’)2 Fragment (2 μg/ml, A11070; Molecular Probes), goat anti-mouse Alexa Fluor 488 F(ab’)2 Fragment (2 μg/ml ...
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bioRxiv - Microbiology 2021Quote: ... Activation of CD4+ T cells was achieved by addition of 25 U/ml of interleukin-2 (IL-2) and CD4+ T-cell activation Dynabeads (Life Technologies). CD4+ T-cells activated for 4-5 days were used for HIV-1 infections ...
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bioRxiv - Biophysics 2022Quote: ... the glass coverslips were mounted onto a Warner chamber and the cells were loaded with 2 µM Fura-2/AM (Molecular Probes) in Ca2+ Imaging Buffer (Ca2+ IB;10 mM HEPES ...
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bioRxiv - Molecular Biology 2020Quote: ... RD18 and JR1 cells were cultured for 2-5 days in DMEM supplemented with 2% horse serum (Gibco, Carlsbad, CA, USA) at 90-95% confluency ...
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bioRxiv - Cell Biology 2020Quote: ... cell pellet of 1 x106 – 2 x106 PBMCs was resuspended in 2 ml erythroblast cell expansion media containing basal blood media Stempo34 SFM (Thermo Fisher) supplemented with 1x Stempo34 nutrient supplement (Thermo Fisher) ...
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bioRxiv - Immunology 2020Quote: T or B cells (2-4 × 107) obtained from 2 spleens of actin-GFP mice by negative MACS kits (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2019Quote: ... were cultured to a density between 2*105 and 2*106 cells per ml in complete media [RPMI 1640 with HEPES and GlutaMAX (Gibco #72400120), supplemented with 10% heat-inactivated Fetal Bovine Serum (FBS) ...
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bioRxiv - Immunology 2023Quote: ... cells were stained at 37°C for 30 min with 2 µM 2’,7’-dichlorodihydrofluorescein diacetate (H2DCF-DA; Thermo Fisher Scientific) or 1 µM MitoSOX (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... The culture dish was coated with 0.2% gelatin and the cells were cultured in endothelial growth medium (EGM)-2 complete media (#CC-3162, Lonza Clonetics, Fisher scientific) with EGM-2 SingleQuots supplement kit (CC-4176 ...
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bioRxiv - Physiology 2024Quote: ... only 4 wells of cells were incubated in DMEM with 4 μM fura-2/AM (fura-2/AM, Molecular Probes, USA) at 37° C in the dark for 45 min ...
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bioRxiv - Immunology 2024Quote: ... Efferocytosis was quantified by incubating BMDMs with Jurkat T cells (MOI 2, 45 min) which had been labelled with calcein-acetoxymethyl (2 h, 37 °C, ThermoFisher Scientific) and exposed to UV light (305 nm ...
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bioRxiv - Cancer Biology 2021Quote: ... the solution was neutralized with 0.5 mL HBSS buffer and 100 μL of the lysed cells were used to determine the protein concentration by the standard Bradford protein assay (Thermo Scientific, Rockford, IL), with bovine serum albumin as a standard.
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bioRxiv - Immunology 2021Quote: ... 10 min) and cell-free supernatants were snap-frozen for total protein analysis using the Pierce™ BCA protein assay kit (Thermo Scientific; #23227). The pellet was resuspended with 400 μL of PBS and total leukocyte count was measured via a hemocytometer (Neubauer chamber) ...
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bioRxiv - Microbiology 2020Quote: ... The results were normalized using total protein content of the cells that was determined using Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific). Experiments have been performed in triplicates in five independent experiments.
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bioRxiv - Evolutionary Biology 2022Quote: ... Total protein concentration in the crude cell lysates was measured using the Pierce™ BCA Protein Assay Kit (Thermo Scientific, Cat. No. 23225). Lysates containing 5 μg of total protein in Laemmli sample buffer were loaded onto a 6% (v/v ...
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bioRxiv - Cancer Biology 2019Quote: The EV fraction or the transfected PCa cells was measured for protein content using the Micro BCA Protein Assay kit (Thermo Scientific, Wilmington, DE). Equal amounts of protein were loaded onto 4-20% Mini-PROTEAN TGX gels (Bio-Rad ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were lysed with 15 μl RIPA buffer and protein concentration was quantified using Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, 23225). OCR measurements were normalized to the protein concentration in each well.
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bioRxiv - Microbiology 2022Quote: ... fluorescent FFE skin cells were collected from the FF using forceps and protein was collected using M-Per™ Mammalian Protein Extraction Reagent (Thermo Fisher Scientific) to be used in immunoblotting or frozen and processed by the University of Illinois Protein Science Laboratory for mass spectrometry (MS)-based analyses.
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bioRxiv - Molecular Biology 2023Quote: ... The whole cell lysate were obtained with RIPA and the protein concentration was measured using BCA protein assay kit (23225, Thermo Scientific, MA, USA). Monoclonal antibody anti-DNMT1 (ab188453 ...
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bioRxiv - Microbiology 2022Quote: Total protein concentration in the crude cell lysates was measured using the Pierce™ BCA Protein Assay Kit (Thermo Scientific, Cat. No. 23225). The lysates were loaded in the amount of 5 µg of total protein in Laemmli sample buffer onto a 6% (v/v ...
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bioRxiv - Immunology 2023Quote: ... The total isolated protein from splenic CD4+CD44+ cells was quantified using Bicinchoninic acid (BCA) Protein Assay Kit (Pierce, Thermo Scientific, USA, #23225).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Every gel included two molecular-weight markers for separate regions of interest (High Range Color-Coded Prestained Protein Marker; Cell Signaling Technology; and Novex Prestained Protein Standard, Thermo Fisher) on opposite ends of the gel ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein concentration from whole cell lysate was measured by BCA assay using Pierce™ BCA Protein Assay Kit (Cat# 23227, Thermo Scientific™) as per manufacturer protocol and 40 µg of protein per well was separated on 12% SDS PAGE ...
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bioRxiv - Cell Biology 2024Quote: ... Following treatment cells were then transduced with Premo™ Autophagy tandem sensor Red Fluorescent Protein-Green Fluorescent Protein (RFP-GFP)-LC3B (Life Technologies, Invitrogen) and chloroquine was used as control ...
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bioRxiv - Cell Biology 2024Quote: ... The protein concentration of the membrane fractions and whole cell lysates were measured using the Pierce BCA protein assay kit (Thermo Fisher Scientific, 23227). Myc-tag immunoprecipitation (IP ...
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bioRxiv - Microbiology 2024Quote: ... The membrane proteins of AGS cells were isolated with the Mem-PER™ Plus Membrane Protein Extraction Kit (Thermo Fisher Scientific, United States). Briefly ...
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bioRxiv - Cell Biology 2023Quote: ... cells were lysed in Ripa Buffer and total protein was quantified using the Pierce™ BCA Protein Assay Kit (Thermo Scientific™, 23227) for data normalization.