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Citations for Thermo Fisher :
1651 - 1700 of 10000+ citations for Dengue Virus Serotype 2 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... Binding of the full-length S protein was detected by mouse anti-His IgG (Invitrogen MA121315, 2 μg/ml) and Alexa Fluor-488-labelled goat anti-mouse IgG (Invitrogen A11001 ...
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bioRxiv - Biochemistry 2020Quote: StNFeoB proteins (2-5 µM each) were incubated with a range of substrate (GTP for molecular biology, Thermo Scientific) concentrations (25 µM to 5000 µM) ...
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bioRxiv - Cell Biology 2021Quote: ... Bound proteins were eluted by boiling in 25 μL of 2×LDS sample buffer (NuPAGE LDS Sample Buffer, Invitrogen). Anti-Protein A Western blot was performed to detect sumoylated Mcm21 or Ctf3 species in the purified material ...
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bioRxiv - Biochemistry 2022Quote: ... the separated proteins were transferred onto polyvinylidene difluoride (PVDF) membrane using iBlot™ 2 Dry Blotting System (ThermoFisher Scientific). The blot was blocked with 5% skim milk in 1xPBS and probed with the anti-mono-ADP-ribose binding reagent/antibody MABE1076 (α-MAR) ...
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bioRxiv - Genomics 2022Quote: ... diluted chromatin was incubated for 2 h with antibodies (2.5 μg/100 μL) coupled to magnetic Dynabeads Protein A (Invitrogen). ChIP samples were washed 4 times in ice-cold RIPA buffer ...
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bioRxiv - Immunology 2022Quote: ... Biotinylated-SARS-CoV-2 tri-S proteins were mixed with FITC-labelled NeutrAvidin beads (1 µm, Thermo Fisher Scientific) (1 µg of tri-S for 1 µl of beads) ...
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bioRxiv - Developmental Biology 2019Quote: ... Lysates were then thawed on ice and 2 μL were used to perform a BCA protein assay (ThermoFisher, # 23225). 10 μL of lysate were diluted 1:1 in 1X MSD lysis buffer and added to the 96-well Mesoscale assay plate ...
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bioRxiv - Microbiology 2021Quote: ... TATA-box binding protein (TBP) (Hs00427620_m1) and beta-2-microglobulin (B2M) (Hs00187842_m1) was used (all from Thermo Fisher Scientific). PCR was performed using the LightCycler 480 Instrument (Roche Life Science ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 spike protein (kindly provided by Eric Fischer, DFCI) was coupled to fluorescent streptavidin beads (Thermo Fisher) and incubated with diluted plasma samples to allow antibody binding to occur ...
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bioRxiv - Microbiology 2021Quote: ... in 6 well plates were transiently transfected with 2μg of either wild-type or mutant SARS-CoV-2 S protein plasmid with Lipofectamine 3000 (Invitrogen) at a ratio of 1:2:2 DNA ...
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bioRxiv - Biochemistry 2020Quote: ... the separated proteins were transferred onto polyvinylidene difluoride (PVDF) membrane using iBlot™ 2 Dry Blotting System (ThermoFisher Scientific). The blot was blocked with 5% skim milk in PBS containing 0.05% Tween-20 and probed with anti-mono or poly ADP-ribose binding reagents/antibodies MABE1076 (α-MAR) ...
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bioRxiv - Molecular Biology 2020Quote: ... The protein-antibody complex was developed by adding 2-3ml of SuperSignal West Pico Chemiluminiscent substrate (Thermo Fisher, USA).
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bioRxiv - Plant Biology 2022Quote: Protein samples were reduced with Tris(2-carboxyethyl)phosphine (TCEP) and cysteines alkylated with Iodoacetamide (IAA, Thermo Fisher, Germany). Subsequent proteolytic digests were performed using S-TRAPs (Protifi ...
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bioRxiv - Microbiology 2022Quote: ... COS-7 cells grown on 13 mm cover slips were transfected with either the empty pcDNA3.1(+) vector or one expressing the SARS-CoV-2 E protein using Turbofect transfection reagent (ThermoFisher). At 24 h pt ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were then transferred on nitrocellulose membranes using the iBlot 2 Dry Blotting System (Invitrogen, Cat# IB21001 and IB23001). The high molecular weight protein GCN1 was transferred using the Trans-Blot Turbo Transfer System (Bio-Rad ...
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bioRxiv - Cancer Biology 2023Quote: ... Oxidized proteins were visualized using the Thermo Scientific Pierce ECL 2 Western Blotting Substrate (Thermo Fisher Scientific, Cat. 80196) and scanned ...
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bioRxiv - Neuroscience 2023Quote: ... were incubated for 2 h at RT and the proteins were detected with Supersignal West Pico (34580, Thermo Scientific) and imaged by using iBright 1500 (Invitrogen) ...
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bioRxiv - Microbiology 2023Quote: ... and the insect cell codon-optimized RBD at residues 330 to 521 of SARS-CoV-2 S protein (GenBank accession no. MN908947.3) was cloned into a pFastBac plasmid (Invitrogen). For the RBD-LTA fusion protein expression ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were transferred onto a nitrocellulose membrane using iBlot 2 gel transfer device (7 min at 25 V, ThermoFisher). Membranes were incubated for 1 hour at room temperature (RT ...
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bioRxiv - Developmental Biology 2023Quote: ... Equivalent amounts of protein (∼2 mg) were used for immunoprecipitation with Pierce anti-c-Myc Agarose (Thermo Scientific, 20169), anti-Flag Affinity Gel (Bimake ...
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bioRxiv - Immunology 2022Quote: ... 2 µg/ml spike protein diluted in PBS was incubated over-night on 96-well high affinity plates (Nunc). To prevent unspecific binding ...
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bioRxiv - Microbiology 2023Quote: ... 2 ug of EV protein content were separated on a 4-12% Bis-Tris gel (Thermo Scientific, Rockford, IL), then blotted onto a PVDF membrane ...
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bioRxiv - Microbiology 2023Quote: ... the sections were incubated with SARS-CoV-2 antibody against the nucleocapsid (N) protein (ThermoFisher MA536086; 1:28,000 dilution) for 15min at room temperature ...
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bioRxiv - Bioengineering 2023Quote: ... FAM-protein conjugates were then purified with Zeba 2 mL spin desalting columns with 40 kDa MWCO (Thermo Scientific) to remove excess unreacted FAM-NHS according to manufacturer’s instructions ...
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Entorhinal cortex vulnerability to human APP expression promotes hyperexcitability and tau pathologybioRxiv - Neuroscience 2023Quote: ... 2/3rd of the eluted protein samples were resolved on a 4–12% Bris-Tris gel (Invitrogen: cat# NW04125box) and subjected to Silverstein (Pierce™ Silver Stain Kit ...
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bioRxiv - Plant Biology 2023Quote: ... and 0.01% [v/v] 2-mercaptoethanol) and then quantified with the Pierce BCA protein assay kit (Thermo Fisher Scientific). The total proteins were separated by 8%-12% SDS-PAGE gels and blotted onto an Immune-Blot polyvinylidene difluoride (PVDF ...
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bioRxiv - Microbiology 2024Quote: ... For the SARS-CoV-2 staining a polyclonal rabbit anti-SARS Coronavirus Spike protein (PA1-41142, Thermo Fisher, France) and revealed using an anti-rabbit IgG (H+L ...
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Undruggable oncoproteins cMyc and NMyc bind to mediator of transcription with superior high affinitybioRxiv - Biochemistry 2024Quote: ... The immuno-detection of proteins was carried out using mouse anti-HA monoclonal antibody (2-2.2.14, #26183, ThermoFisher Sci) and secondary anti-mouse IgG antibodies conjugated with horseradish peroxidase (#A9044 ...
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bioRxiv - Cell Biology 2024Quote: ... Immunoprecipitation was performed by adding 2 mg of P-CREB antibody to protein A/G Dynabeads (Life Technologies, USA) and incubating the mixture overnight at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... the separated proteins were transferred onto polyvinylidene difluoride (PVDF) membrane using iBlot™ 2 Dry Blotting System (ThermoFisher Scientific). The blot was blocked with 5% skim milk in PBS containing 0.05% Tween-20 and probed with anti-mono ADP-ribose binding reagent MABE1076 (α-MAR ...
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bioRxiv - Microbiology 2020Quote: Concentrated virus samples were mixed with Novex™ Tris-Glycine SDS Sample Buffer (2X) (Thermo Fisher Scientific) with NuPAGE™ Sample Reducing Agent (10X ...
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bioRxiv - Cancer Biology 2020Quote: ... RNA was reverse transcribed (RT) using Moloney murine leukemia virus reverse transcriptase (Ambion, Life Technologies, Carlsbad, CA) and a mixture of anchored oligo-dT and random decamers (Integrated DNA Technologies ...
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bioRxiv - Cancer Biology 2020Quote: ... RNA was reverse transcribed (RT) using Moloney murine leukemia virus reverse transcriptase (Ambion, Life Technologies, Carlsbad, CA) and a mixture of anchored oligo-dT and random decamers (Integrated DNA Technologies ...
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bioRxiv - Neuroscience 2021Quote: ... the virus solution was typically mixed at 1:500 with blue beads (fluorescent polymer microspheres, Thermo Scientific).
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bioRxiv - Microbiology 2020Quote: ... Reovirus S4 RNA was quantified using the Taq-Man Fast Virus One-Step Master Mix (Applied Biosystems) and GAPDH was detected as an endogenous control using the Pre-Developed TaqMan Assay Reagent kit for mouse GAPDH (Applied Biosystems ...
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bioRxiv - Microbiology 2020Quote: ... reverse transcription was performed with random hexamers and Moloney murine leukemia virus (M-MLV) reverse transcriptase (Invitrogen). Synthesized RNA was used as a standard (BEI) ...
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bioRxiv - Microbiology 2020Quote: ... centrifuged and suspended in virus media that is composed of DMEM high glucose (Thermo Fisher Scientific, USA), with 2% fetal bovine serum (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... Master mix for qRT-PCR was prepared using TaqMan Fast Virus 1-step Master Mix (ThermoFisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: Genomic DNA was extracted from 1010 virus particles using the PureLink TM Genomic DNA mini kit (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... iPSCs were generated by Sendai virus reprogramming and clonal expansion using the CytoTune – iPSC 2.0 kit (ThermoFisher) to deliver the reprogramming factors ...
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Map7D2 and Map7D1 facilitate microtubule stabilization through distinct mechanisms in neuronal cellsbioRxiv - Cell Biology 2022Quote: Each RNA sample was subjected to reverse transcription using murine leukemia virus reverse transcriptase (Thermo Fisher Scientific), and the generated cDNA was used as a template for qRT-PCR ...
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bioRxiv - Immunology 2021Quote: ... Reactions were performed using TaqMan® Fast Virus 1-Step Master Mix (Thermo Fisher Scientific, Waltham, USA) and 5 μl of isolated RNA as a template ...
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bioRxiv - Microbiology 2020Quote: ... Isolated ZIKV RNA was titrated by qRT-PCR using TaqMan Fast Virus 1-Step Master Mix (ThermoFisher) and a LightCycler 480 or LC96 instrument (Roche) ...
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bioRxiv - Microbiology 2021Quote: Virus stock were diluted in 10-fold serial dilutions in 2ml Opti-MEM I (1X) + GlutaMAX (Gibco). 1 ml of each dilution was added to monolayers of 2 × 106 VeroE6 cells in the same medium in a 6-well plate ...
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bioRxiv - Biochemistry 2020Quote: ... The cDNAs were synthesized using Moloney murine leukemia virus RT with random primers (Invitrogen, Thermo Fisher Sci.). RNA levels of representative transcripts were analyzed in triplicate by quantitative PCR with SYBR™ Green using a Bio-Rad CFX thermal cycler ...
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bioRxiv - Biochemistry 2020Quote: ... The cDNAs were synthesized using Moloney murine leukemia virus RT with random primers (Invitrogen, Thermo Fisher Sci.). RNA levels of representative transcripts were analyzed in triplicate by quantitative PCR with SYBR™ Green using a Bio-Rad CFX thermal cycler ...
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bioRxiv - Immunology 2021Quote: ... Reactions were conducted with 5 μL RNA and TaqMan Fast Virus 1-Step Master Mix (Applied Biosystems) with 500 nM primers and 200 nM probes ...
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bioRxiv - Neuroscience 2021Quote: ... The genomic titer of each virus was determined by quantitative PCR using the ABI 7700 (Applied Biosystems) and primers specific to the WPRE ...
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bioRxiv - Immunology 2020Quote: ... Virus detection was performed using One-Step RT-PCR SuperScript(tm) III Platinum(tm) Kit (Life Technologies). Thermal cycling was achieved at 55°C for 10 minutes for reverse transcription ...
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bioRxiv - Microbiology 2022Quote: ... and plasmids encoding two vaccinia virus capping enzyme subunits (pCAG-D1R and pCAG-D12L −0.8μg each) using 16μL Lipofectamine 2000 (Invitrogen) per transfection reaction in a total volume of 200μL of Opti-MEM (Gibco) ...