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Citations for Thermo Fisher :
1801 - 1850 of 10000+ citations for 5 Isobutylcyclohexane 1 3 dione 98% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... at a ratio of 1:3 (µg DNA: µg PEI) in 200 µl Opti-MEM (Gibco, 31985070). Media was replaced after 24h ...
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bioRxiv - Cell Biology 2023Quote: Cells were washed 2x with PBS and gently dissociated with 1:3 diluted Accutase (Gibco, A11105-01). Lifted cells were washed 2x with cold dPBS (HyClone ...
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bioRxiv - Neuroscience 2023Quote: ... the slices were incubated in Cyanine 3 (Cy3) goat anti-mouse (Thermo Fisher Scientific, A10521, 1:500) in PBS-Triton X 0.3% ...
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bioRxiv - Molecular Biology 2024Quote: ... complexes (DNA to PEI MAX ratio, 1:3, w/w) formed in Opti-MEM (Thermo Fisher Scientific) via incubation for 15 min at 25°C ...
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bioRxiv - Molecular Biology 2024Quote: ... counted and total RNA was recovered from 3×106 cells using 1 ml of Trizol (ThermoFisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2024Quote: ... 150ng of DNA was run on 3 lanes of a 1% eGel (Thermo Fisher Scientific, Waltham, MA) and size-selected into two populations ...
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bioRxiv - Bioengineering 2024Quote: ... for 1 hour before blocking with 3% (w/v) bovine serum albumin (BSA, Fisher Scientific, Cat # BP9703100). To detect ARP3 and GLUT4 ...
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bioRxiv - Genomics 2024Quote: ... cells were transfected with 1 µg of plasmid library using 3 µl Lipofectamine 3000 reagent (L3000008, Invitrogen, Carlsbad ...
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bioRxiv - Developmental Biology 2020Quote: ... cells were diluted to less than 1 ×106 cells/mL in PBS with 3% FBS and incubated in 1:100 EpCAM-APC (Invitrogen, 17-5791-80) or 1:100 IgG-APC isotype control (BioLegend ...
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bioRxiv - Molecular Biology 2021Quote: ... Membrane was blocked with 5% milk and probed with anti-mAID (1:5,000, MBL Life Science, cat. No. M214-3) or anti-GAPDH-HRP (1:50,000, Invitrogen, cat. No. MA5-15738-HRP). Membranes were visualized with 0.5x ECL (Amersham ...
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bioRxiv - Bioengineering 2020Quote: ... RT-qPCR was performed on a 1:3 dilution of the extracted RNA using TaqPath™ 1-Step RT-qPCR Master Mix (Thermo Fisher Scientific) with the following forward and reverse primer sequences ...
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bioRxiv - Cell Biology 2021Quote: ... 3 x 1 h) and incubated with the secondary antibody (Alexa-labelled goat anti-mouse 555 plus, A32727, Thermo Fisher Scientific, 1:1000), an anti-GFP antibody conjugated with Alexa 488 (A-21311 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Nepa Gene) with 3 µg plasmid DNA and 1 × 106 cells in 100 µl Opti-MEM (1×) Reduced Serum Medium (Gibco® ThermoFisher Scientific). The electroporated cells were cultured in a non-selective complete medium in a 100 mm × 20 mm cell culture dish (Falcon ...
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bioRxiv - Cancer Biology 2020Quote: ... The assembled PDMS layers were treated with oxygen plasma (100 watt, 1 ccm, 140 torr, 10 seconds) for irreversible bonding to a glass slide (Fisherbrand 1×3”, Fisher Scientific, Pittsburgh, PA). Prior to flow experiments ...
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bioRxiv - Neuroscience 2022Quote: ... samples were washed three times for 3 minutes and then incubated 1 hour at RT with secondary antibody (1:500, #A21202, #A21449, #A10042, #A32731, Life Technologies, Carlsbad, California) and Hoechst 33342 (1:1000 ...
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bioRxiv - Biochemistry 2022Quote: ... The wells were washed with 1% BSA/LBB and incubated for one hour with L9393 (1:5000 dilution) in 3% BSA/LBB followed by incubation with Horseradish Peroxidase (HRP)-conjugated anti-rabbit IgG (Invitrogen, 1:5000 dilution) in 3% BSA/LBB for 30 minutes ...
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bioRxiv - Immunology 2023Quote: ... Deposited ECM was fixed by exposure to 100% methanol at −20°C and stained with Alexa Fluor488-conjugated anti-fibronectin (EBioscience, clone FN-3, 1:500 dilution) or Alexa Fluor594-conjugated (Invitrogen, 1:1000 dilution) anti-ColI/III antibodies (EMD ...
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bioRxiv - Molecular Biology 2024Quote: ... Brain slices were blocked with 5% donkey serum in 0.3% PBS-T for 1 h at RT and then incubated with a sheep anti-GFRAL antibody (1:500, Thermofisher, Cat. PA5-47769) and/or a rabbit anti-c-Fos antibody (1:500 ...
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Direct analysis of ribosome targeting illuminates thousand-fold regulation of translation initiationbioRxiv - Molecular Biology 2020Quote: ... and 3′ biotinylated using the Pierce RNA 3′end biotinylation kit (Thermo Scientific 20160).
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bioRxiv - Systems Biology 2019Quote: 3’-tRNAs biotinylation was adapted from Pierce RNA 3’-End Biotinylation Kit (Thermo Fisher). Deacylated tRNAs were denaturated in 25% DMSO at 85°C for 5 minutes and directly chilled on ice ...
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bioRxiv - Cell Biology 2021Quote: ... 3 μl were sampled on a 3 well Diagnostika slides (X1XER303B) from Thermo scientific for observation on an Zeiss LSM710 confocal microscope equipped with a Plan-Apochromat 63×/1.4 Oil objective and 405 nm and 488 nm lasers ...
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bioRxiv - Microbiology 2023Quote: ... 3′RNA-seq libraries were analyzed on a Qubit 3 Fluorometer (Thermo Fisher Scientific) and an Agilent 4200 TapeStation System prior to paired- end sequencing using the HiSeq 2500 system (Illumina).
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bioRxiv - Neuroscience 2022Quote: ... 3 μg DNA and 3 μL Lipofectamine in 300 μl Optimem (Thermofisher Scientific, USA) were used per well containing 700 μl DMEM ...
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bioRxiv - Neuroscience 2024Quote: ... wells were treated with Caspase-3/7 (CellEvent™ Caspase-3/7 Green, Invitrogen) 1:1000 in treatment media ...
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bioRxiv - Microbiology 2019Quote: ... 1 μg of this plasmid was transfected into 5×105 SH-SY5Y cells with Lipofectamine LTX PLUS (1:1 ratio; Invitrogen) and selected for colonies with G418 (0.5 mg/ml) ...
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bioRxiv - Developmental Biology 2020Quote: ... and cultured at 37 °C in a humidified 5% CO2 atmosphere with kidney culture medium (F12:DMEM (1:1) + Glutamax (Gibco) supplemented with 10% fetal bovine serum and penicillin-streptomycin)(Ihermann-Hella & Kuure ...
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bioRxiv - Microbiology 2019Quote: ... A 2 μl volume of cDNA diluted 1:4 (IAV) or 1:5 (reovirus) in molecular biology grade water (Invitrogen) was added to the 384 well plate ...
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bioRxiv - Systems Biology 2019Quote: ... 25 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 1% NP-40 and 5% glycerol; Thermo Fisher) containing 1x protease inhibitor cocktail III (Calbiochem) ...
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bioRxiv - Bioengineering 2020Quote: ... The cells were passaged at a density of 1:2 to 1:8 after reaching ~80% confluency by detaching with 5 mM EDTA in PBS (Invitrogen 15575-038 diluted in sterile 1X PBS ...
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bioRxiv - Cancer Biology 2021Quote: ... Slides were blocked in 1% BSA and then stained with indicated antibodies against Claudin-5 (Invitrogen 35-2500 1:50), Ad5 (Abcam ab6982 1:100) ...
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bioRxiv - Neuroscience 2020Quote: ... with 1% PBS-T (1% Triton X-100 in 1x PBS) and blocked in 1% PBS-T containing 5% normal goat serum (ThermoFisher) for one hour ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were resuspended in 1 ml IP lysis buffer [25 mM Tris-HCl pH 7.5 / 150 mM NaCl / 1% NP-40 / 1 mM EDTA / 5% glycerol / Halt protease inhibitor cocktail (Thermo scientific) / Halt phosphatase inhibitor cocktail (Thermo scientific)] and incubated on ice for 1 h ...
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bioRxiv - Microbiology 2019Quote: ... at a 1:10000 dilution in 5% skimmed milk-PBST and 1:20000 secondary HRP Rabbit-anti-mouse antibody (Invitrogen) in 5% skimmed milk-PBST16.
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bioRxiv - Genomics 2019Quote: ... Cells were resuspended in 1 ml IP lysis buffer [25 mM Tris-HCl pH 7.5 / 150 mM NaCl / 1% NP-40 / 1 mM EDTA / 5% glycerol / Halt protease inhibitor cocktail (Thermo scientific) / Halt phosphatase inhibitor cocktail (Thermo scientific)] and incubated on ice for 1 h ...
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bioRxiv - Immunology 2021Quote: ... 1.75x103 cells were loaded into individual well of a 96 well v-bottom plate in RPMI/5% (v/v) FBS/2mM EDTA/1% (v/v) Penicillin/Streptomycin/1% (v/v) L-Glutamine (ThermoFisher)/0.1% (v/v ...
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bioRxiv - Developmental Biology 2020Quote: ... 25 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 1% NP-40 and 5% glycerol; Thermo Fisher) containing 1x protease inhibitor cocktail III (Calbiochem ...
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bioRxiv - Cell Biology 2020Quote: ... 0.1 M MgCl2, 0.1M Tris HCL, 1% Tx-100, 1x sBlock, 1% sodium deoxycholate, 5 units/mL DNAse I – Thermo Scientific #89836 ...
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bioRxiv - Systems Biology 2021Quote: ... Native protein complexes from platelets were obtained by incubating the washed pallet with Pierce IP lysis buffer (25 mM Tris•HCl pH 7.4, 150 mM NaCl, 1% NP-40, 1 mM EDTA, 5% glycerol) (Thermo Scientific) containing protease and phosphatase inhibitors at 4 °C for 5 minutes ...
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bioRxiv - Pathology 2021Quote: ... pellets were resuspended in 50μl of 5% SDS containing phosphate buffered saline and cytoplasmic fractions were mixed 1:1 with 10% SDS solution (Thermofisher) to give a final 5% SDS concentration ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated in culture media (66% BME, 25% Hanks, 5% FBS, 1% N-2, 1% penicillin, streptomycin and glutamine; all from Invitrogen) and 0.66% D-(+)-Glucose (Sigma ...
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bioRxiv - Biochemistry 2022Quote: ... SK-N-BE(2)-C were grown at 37 °C and 5% CO2 in a media containing 1:1 mixture of EMEM (ATCC) and F12 medium (Gibco) supplemented with 10% fetal bovine serum (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... were coated with 100 µL of recombinant SARS-CoV-2 S protein (Wuhan-1 strain and BA.5) at a concentration of 1 µg/mL in PBS (Gibco) at 4°C overnight ...
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bioRxiv - Physiology 2023Quote: ... 0.5 % non-fat dry milk the membrane was incubated overnight with rabbit polyclonal anti-calstabin 1 (PA 1-026, ThermoFisher) (1:1200 in blocking buffer) ...
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bioRxiv - Physiology 2023Quote: ... with a 1:5 – 1:10 passaging rate onto cultureware coated with Geltrex (LDEV-Free, hESC-Qualified; Gibco, Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... and resuspended with 5 mg/mL collagenase and 1 mg/mL DNase I in 1 mL of RPMI 1640 without phenol red (Gibco). After incubation at 37°C for a half hour ...
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bioRxiv - Developmental Biology 2024Quote: ... The following primary antibodies were used: Zn-5 (1/200; ZIRC, University of Oregon) and GFP (1/1000; Molecular Probes). After several washes in PBT1% ...
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bioRxiv - Developmental Biology 2024Quote: ... and cultured in SCs culture medium (growth medium: 20% FBS, 5% horse serum, 1% penicillin/streptomycin, 1% GlutaMAX supplement (Gibco) and 2.5 ng/ml FGF (R&D systems ...
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bioRxiv - Synthetic Biology 2023Quote: ... and split 1:5 or 1:10 into a new tissue culture (TC)–treated T75 flask (Thermo Scientific ref 156499).
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bioRxiv - Cancer Biology 2023Quote: ... Cells were also stained for 30 minutes simultaneously with 1 μM 2′-[4-ethoxyphenyl]-5-[4-methyl-1-piperazinyl]−2,5′-bi-1H-benzimidazoletrihydrochloride trihydrate (Hoechst 33342, Fisher Scientific) for nuclear visualization and cell localization ...
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bioRxiv - Developmental Biology 2023Quote: ... coated plates in growth media (DMEM with 20% FBS, 5% horse serum, 1% pen-strep, 1% glutamax (above from Gibco), 0.1% chick embryo extract (MPbio ...