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Citations for Thermo Fisher :
1501 - 1550 of 10000+ citations for 6H Dibenzo b d pyran 3 pentanaminium 6a 7 10 10a tetrahydro 1 hydroxy N N N 6 6 9 hexamethyl 6aR trans 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: Neocortex from C57Bl/6 pups (postnatal day 1-3) was dissected in Hibernate-A medium (#A1247501, Gibco, USA), digested with papain (#LS003124 ...
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bioRxiv - Biophysics 2023Quote: ... in 3% BSA with a 1:50 ratio and 4’,6-diamidino-2-phenylindole (DAPI, Thermo Fisher Scientific) were employed ...
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bioRxiv - Neuroscience 2024Quote: After a wash in Carnoy’s solution (6 ethanol: 3 chloroform: 1 acetic acid; all from Thermo Fisher, USA), used to removed most of the lipids from the section [45] ...
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bioRxiv - Cell Biology 2022Quote: ... MCF-10A or GFP-LGN-expressing MCF-10A cells were plated in glass bottom dishes (Nunc). Prior to imaging ...
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bioRxiv - Genomics 2024Quote: ... for 6h using lipofectamine 3000 (Invitrogen). Knockdown was confirmed by RT-qPCR at 24-72h post-transfection.
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bioRxiv - Cancer Biology 2020Quote: ... Proton transport was measured using ATP-dependent quenching of 9-amino-6-chloro-2-methoxy-acridine (Acridine Orange, ThermoFisher) fluorescence quenching for isolated vacuoles as previously described21
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bioRxiv - Microbiology 2023Quote: ... live/dead staining was carried out using 6 μM syto 9 and 30 μM propidium iodide (BacLight, Molecular Probes). Biofilms were imaged using a Zeiss LSM 700 compact confocal laser scanning microscope using the 40x objective and appropriate fluorescence settings (syto 9=488 nm laser ...
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bioRxiv - Molecular Biology 2024Quote: WT and TRBP−/− HeLa cells were plated at 4 x 106 cells / dish into a 9-cm dish and transfected with 6 µg/dish poly(I:C) using Lipofectamine 2000 (Invitrogen). At 0 ...
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bioRxiv - Cell Biology 2019Quote: ... First antibody incubation was done O/N at 4°C and the secondary HRP-coupled antibodies goat anti-Mouse IgG (H+L) (ThermoFisher scientific; 31430; 1:10,000) or polyclonal HRP-conjugated goat-anti-rabbit IgG (ThermoFisher scientific ...
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bioRxiv - Neuroscience 2023Quote: ... for 2 h RT and incubated o/n with a primary antibody cocktail containing anti-GFP antibody (polyclonal chicken anti-GFP, 1:2000; #A10262, AB_2534023; Thermo Fisher Scientific, Rockford, IL, USA), anti-TH antibody (TH polyclonal rabbit anti-mouse ...
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bioRxiv - Cancer Biology 2023Quote: ... Whole-cell lysates were prepared from 5x106 cells treated with N-acetylcysteine (MilliporeSigma A7250) for 4 hours followed by stimulation/oxidation with 1 mM hydrogen peroxide (Fisher Scientific Canada, Mississauga ON) for 5 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... The samples were subjected to 6 rounds of 10 sec sonication each at power 3 in a 550 Sonic Dismembrator (Fisher Scientific). Between each round of sonication ...
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bioRxiv - Cancer Biology 2021Quote: ... was validated by STR analysis using AmpFLSTR™ Identifiler™ Plus PCR Amplification Kit (Cat n:4486467, ThermoFisher Scientific) using the provided protocol ...
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bioRxiv - Neuroscience 2021Quote: ... The cDNA encoding the short transcriptional variant of human DA D2 receptor with an N-terminal FLAG tag (SF-D2R-S) was inserted into mammalian expression vector pcDNA 3.1(+) (Invitrogen)62 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... over the course of 28 days and healthy controls (n = 37) and hybridized onto an HU133 Plus 2.0 GeneChip (Affymetrix) according to the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2020Quote: ... GFP-N was immunoprecipitated from two independent HEK293 cell pellets using 5μg of anti-GFP antibody (Life Technologies G10362). A total of 2% input material was obtained for size-matched control libraries (SMI ...
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bioRxiv - Neuroscience 2019Quote: ... Cortical hemispheres were dissected and homogenized in ice-cold homogenization buffer (N-PER Neuronal Protein Extraction Reagent (ThermoFisher Scientific) with cOmplete Protease Inhibitor Cocktail (Roche) ...
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bioRxiv - Developmental Biology 2019Quote: ... GenBank accession number BC057894.1) with an N-terminal hemagglutinin (HA) tag were reverse transcribed with SuperScript III Reverse Transcriptase (Invitrogen) and PCR-amplified using Phusion high-fidelity DNA polymerase (New England Biolabs ...
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bioRxiv - Bioengineering 2021Quote: Each sample (n=5) was placed in a 2 mL centrifuge tube containing 320 μL of collagenase II (Gibco, Thermo Fisher Scientific Inc. ...
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bioRxiv - Biochemistry 2020Quote: ... NosRcaΔC and NosRca-IFmut was labelled at the N terminus with the fluorophore Alexa Fluor 405 NHS ester (ThermoFisher) (∼2.2 ...
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bioRxiv - Neuroscience 2020Quote: ... Alexa Fluor® 555 goat anti rabbit IgG (Cat. N° A21429) was obtained from Life Technologies (Carlsbad, CA, USA), Alexa Fluor® 488 Goat Anti-Mouse IgG (Cat ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... over the course of 28 days and healthy controls (n = 37) and hybridized onto an HU133 Plus 2.0 GeneChip (Affymetrix) according to the manufacturer’s recommendations ...
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bioRxiv - Plant Biology 2021Quote: ... Tyr-nitration of MSD2 was detected after SDS-PAGE with anti-Nitrotyrosine primary antibody (Invitrogen, cat. n. A-21285) diluted to 1:3,000 in TBST with 3% (w/v ...
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bioRxiv - Cell Biology 2021Quote: ... and the N-terminus of the construct was attached to a biotinylated DNA-coated paramagnetic bead (2.8 μm diameter, Invitrogen) via a biotin-neutravidin interaction ...
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bioRxiv - Biophysics 2020Quote: A1-LCD was fluorescently labeled on the N-terminus using Alexa Fluor® 488 NHS ester fluorescent dye (ThermoFisher) to label the primary amines of proteins ...
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bioRxiv - Microbiology 2022Quote: ... The pellet containing the membranes was resuspended in 20 mM phosphate buffer pH 7.4 with 0,3% n-octyl-poly-oxyethylene (OPOE) detergent (Affymetrix, UK) and incubated at 20°C for 2 h under agitation to separate inner (IM ...
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bioRxiv - Cell Biology 2022Quote: HEK 293T cells were transfected with 1 µg of plasmids containing either FL (wild-type), various deletions (ΔTM, ΔNΔTM, NCC, CC, N) or mutant FLAG-KASH5 constructs with Lipofectamine LTX (Invitrogen) transfection reagent using the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Taqman gene expression assays for N2 and N3 regions of the SARS-CoV-2 N gene were made (ThermoFisher) following CDC specifications (Division of Viral Diseases ...
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bioRxiv - Immunology 2021Quote: ... of M13KO7 helper phage and grown o/n at 37°C in fresh 2xYT medium supplemented with Amp100 and 75μg/mL of kanamycin (Kan75, Gibco) to select for bacteria containing a phagemid and infected with a helper phage ...
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bioRxiv - Molecular Biology 2020Quote: ... The peptides were separated on an in-house made C18 column (20 cm length, 75 μm inner diameter, 1.9 μm particle size) by an easy n-LC 1200 (ThermoFisher) and directly injected in a QExactive-HF or in case of TMT samples into a Fusion Lumos mass-spectrometer (ThermoFisher ...
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bioRxiv - Microbiology 2022Quote: ... offline high pH reverse-phase fractionation was performed using a Hypersil Gold column (Thermo Scientific, P/N 25002-202130). The mobile phase A was water adjusted with ammonium hydroxide to pH 10 and B was 10 mM ammonium bicarbonate in 80% Acetonitrile (ACN ...
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bioRxiv - Genomics 2022Quote: ... An N-terminal FLAG tag and a nuclear localization signal (NLS) along with the M.CvIPI ORF were then cloned N-terminal to Lamin B1 into pENTR (Invitrogen) using Gibson cloning (New England Biolabs) ...
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bioRxiv - Cancer Biology 2022Quote: ... 2 million SK-N-BE(2)-FLUC cells were stained immediately before injection with CellTrace™ Far Red (Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... The gel was then transferred to Hybond N+ (Amersham) nylon membrane and blotted with HRP-Conjugated Streptavidin (Thermo Scientific) with a dilution factor 1:2000 ...
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bioRxiv - Genomics 2019Quote: ... two similar arrays were used for targeted genotyping within the study population: the UK BiLEVE Axiom Array (n=49,950) by Affymetrix and the UK Biobank Axiom Array (n=438,427) ...
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bioRxiv - Genetics 2021Quote: ... We dissected the facial primordia (n = 5 from each breed) and stored the tissue in RNAlater (Thermo Fisher Scientific) at −80°C ...
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bioRxiv - Biochemistry 2021Quote: ... the Lambda DNA in TE from Quant-iT ™ PicoGreen® dsDNA Assay Kit (ThermoFisher Scientific, cat. n. P11496) was used ...
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bioRxiv - Immunology 2020Quote: The validation cohort included a collection of selected G1 and G3 ccRCC tissues (n = 14 each) and corresponding normal kidney tissue (n = 14) microarray data (“Rostock cohort”, Array GeneChip HG U133 Plus 2.0, Affymetrix) (Maruschke et al. ...
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bioRxiv - Neuroscience 2019Quote: ... The antigen peptide with N-terminus Cysteine (NH2-CGGGGAAPGPPRHG-COOH) was coupled with keyhole limpet hemocyanin (Thermo fisher 77600). The antigen was injected into rabbit and antisera were collected after primary and several booster injections ...
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bioRxiv - Biochemistry 2021Quote: ... The construct with a N-terminal 8xHis-TwinStrep-GFP-tag were expressed in GripTite HEK293 MSR cells (Thermo Fisher) in black 96 well plates with transparent bottoms ...
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bioRxiv - Biochemistry 2021Quote: ... H3.3 was cloned into pLEX_305-C-dTAG and p53 as well as RelA were cloned into pLEX_305-N-dTAG using Gateway LR clonase II Enzyme mix kit (Invitrogen). The sequences of the primers are listed below.
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bioRxiv - Immunology 2021Quote: ... Molecular detection of SARS-CoV-2 was performed with TaqMan™ Gene Expression Assay (Cat. N. 4331182, ThermoFisher®) with specific primers/probes previously described40 ...
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bioRxiv - Biochemistry 2022Quote: ... we resuspended the frozen nuclei fractions in 300 μL of N-PER™ Neuronal Protein Extraction Reagent (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2022Quote: Our gene interaction-perturbation pipeline applied the discovery cohort (n =2,167) composed of 19 independent datasets from the same chip platform (Affymetrix Human Genome U133 Plus 2.0 Array ...
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bioRxiv - Immunology 2022Quote: ... and excess N-ethylmaleimide was removed by filtration with Zeba spin columns with a 7kDa cut-off (Thermo Fisher). Sulfenic acid residues were selectively reduced with 200 mM sodium arsenite and newly released free thiols were labeled by adding 200 mM 1mM biotin–maleimide ...
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bioRxiv - Bioengineering 2024Quote: ... Amount of N-Glycan was measured by integrating the peaks with Thermo Xcalibur software (Thermo Fisher Scientific, Waltham, MA) giving the normalized ...
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bioRxiv - Cell Biology 2023Quote: ... pcDNA5/FRT/TO N-YFP vector was double digested by restrictive enzymes of KpnI and NotI (Thermo Fisher Scientific). Wild type Cdc20 was amplified by PCR and inserted into the expression vector according to standard procedures ...
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bioRxiv - Biochemistry 2024Quote: ... and G3BP1 were labeled on their N-termini with AF488 (Alexa Fluor 488 carboxylic acid succinimidyl ester, Thermo Fisher). Storage buffer was exchanged to Labeling buffer (50 mM MES pH 6.5 ...
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bioRxiv - Evolutionary Biology 2024Quote: Genomic DNA of all ancestral (A) and evolved (N) lines were isolated using Yeast DNA Extraction kit (Thermo Scientific) following manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... or SK-N-BE(2) cells were transfected with the siRNAs using Lipofectamine RNAiMAX Transfection Reagent (ThermoFisher Scientific, #13778030) according to the manufacturer’s instructions ...