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Citations for Thermo Fisher :
1601 - 1650 of 10000+ citations for 6H Dibenzo b d pyran 3 pentanaminium 6a 7 10 10a tetrahydro 1 hydroxy N N N 6 6 9 hexamethyl 6aR trans 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... embryos at the 3-6 somite stage were embedded oriented laterally in 1% low-melt agarose (Invitrogen, Carlsbad, CA) and imaged under bright field (to determine yolk elongation by taking major and minor axis measurements ...
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bioRxiv - Developmental Biology 2022Quote: ... and passaged very 3-4 days with a 1:4-6 passage ratio using Versene solution (Life Technologies 15040066).
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bioRxiv - Neuroscience 2019Quote: ... harbouring the full-length cDNA coding for the human muscle 6-phosphofructo-1-kinase muscle isoform (PFK1-M)3 (accession number, NM_000289.1) using Lipofectamine LTX-PLUS Reagent (Life Technologies) according with manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... HCT8 cells were grown on a 6-well slide (3×105/well, Thermo Scientific) overnight ...
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bioRxiv - Cancer Biology 2020Quote: HEK293T cells (3×105) were seeded in a 6 well plate (Nunc,Thermo,USA) with DMEM media (Invitrogen,USA ...
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bioRxiv - Cancer Biology 2019Quote: ... Spheroids pictures were taken after 3 and 6 days using EVOS FL microscope (Thermofisher) and the images were analyzed with ImageJ software ...
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bioRxiv - Microbiology 2023Quote: ... Substrate 2,2’-Azinobis [3-ethylbenzothiazoline-6-sulfonic acid]-diammonium salt (ABTS; Thermo Fisher Scientific) was added ...
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bioRxiv - Biochemistry 2019Quote: ... 6 mM β-mercaptoethanol and 10 U/mL of SuperASE-In (Ambion)) ...
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bioRxiv - Microbiology 2022Quote: ... Electrophoresis was performed with 6% or 10% Novex TBE gels (Thermo Fisher) or in-house 6% polyacrylamide TBE gels ...
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bioRxiv - Genetics 2022Quote: ... Over the course of the following 4 days, the colonies were gradually transitioned to neural induction medium (NIM) (consisting of DMEM/F12, 1% N-2 supplement (Fisher Scientific, Cat #: 17-502-048), MEM non-essential amino acids (Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... Silencer® Select N°1 negative control siRNA or predesigned Silencer® Select siRNAs for ZNF827 (s45694, s45695, s45696) (Thermo Fisher Scientific, Waltham, MA, USA) were used for the transfection process ...
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bioRxiv - Plant Biology 2024Quote: Three replicates of 50 after-ripened mature seeds were sterilized as above and subsequently imbibed on moist nitrocellulose filter papers (Whatman n°1; Thermo Fisher Scientific; Waltham, MA, USA) placed on 55 mm Petri dishes containing solid MS/2 with or without 1 μM 2-cis ...
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bioRxiv - Neuroscience 2024Quote: ... wells were treated with Caspase-3/7 (CellEvent™ Caspase-3/7 Green, Invitrogen) 1:1000 in treatment media ...
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bioRxiv - Cell Biology 2024Quote: Protein concentrations of samples (fractions 6-9) were determined with a Pierce™ BCA Protein Assay Kit (ThermoFisher, Rockford, USA), and Bradford assay (Biorad ...
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bioRxiv - Developmental Biology 2023Quote: ... The precipitated pellets containing the cumulus-oocyte complexes (COCs) were transferred to a 9 cm Petri dish and 6 ml of wash medium (TCM 199 - GIBCO, buffered with 2.5% HEPES ...
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bioRxiv - Cancer Biology 2021Quote: The Asparagine to Glutamine (N-Q) point mutation was performed using a Phusion SDM kit (Thermo Fisher Scientific, CAT# F541). In this assay ...
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bioRxiv - Cell Biology 2020Quote: ... The Flp-In T-Rex HEK293 cells were transfected with the N-terminal GFP-tagged FAM83F packaged in a pcDNA5-FRT/TO vector along with Flp recombinase pOG44 (Invitrogen) in a ratio of 1 μg ...
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bioRxiv - Developmental Biology 2021Quote: The Emerald Green Fluorescent Protein (EmGFP) -encoding region from Vivid ColorsTM cDNA pcDNATM6.2/N-EmGFP-DEST Vector (Thermo Fisher Scientific) was placed upstream of the coding sequence of PmlVI (human PML transcript variant 5 ...
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bioRxiv - Genetics 2021Quote: ... https://www.lifetein.com) polyclonal antisera raised against the N-terminal peptide of CenH3 [66] and anti-Rabbit-Alexa Fluor 488 (ThermoFisher Scientific). KNL2 and CENP-C were detected in root nuclei as previously described [67] using custom made anti-AtKNL2 [37] or anti-AtCENP-C antibodies (http://www.eurogentec.com/ ...
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bioRxiv - Developmental Biology 2021Quote: Lymph node divested mammary glands (excluding the cervical pair) were dissected from the mice and digested O/N in DMEM/F12 (Gibco) + 10 mM HEPES (Gibco ...
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bioRxiv - Neuroscience 2022Quote: Genes encoding the full-length sequences of Human PP2A A subunit and PP2A C subunit with N-terminal His tag and non-cleavable HA tag were sub-cloned into the pFastBac-Dual vector (Invitrogen). Sequences of PP2A A and C subunits are shown in Table 1 ...
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bioRxiv - Plant Biology 2022Quote: ... AtSUMO1 was cloned into destination vector pK7WGR2 (N-terminal mRFP tag, (Karimi et al., 2002)) by LR reactions using Gateway technology (Invitrogen). Vector specific primer p35s-F used in plasmid construction are listed in Table S2.
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bioRxiv - Molecular Biology 2020Quote: ... of “QTARKSTGGKAPRK” related peptides (1xAcetyl [K10]; 1xGln->pyro-Glu [N-Term]; 1xmethylations [K5]) using Qual Browser (Thermo xcalibur 4.1.50, Thermo Fisher Scientific) with ±15ppm width.
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bioRxiv - Molecular Biology 2020Quote: ... at 200 V for 40 min in NuPAGE 2-(N-morpholino)ethanesulfonic acid (MES) running buffer (cat. no. NP0002, Invitrogen), supplemented with NuPAGE antioxidant (cat ...
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bioRxiv - Genomics 2020Quote: ... a puromycin N-acetyltransferase and the WPRE next to stuffer sequences was ordered as a GeneArt Synthetic Gene (Life Technologies) and PCR-amplified ...
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bioRxiv - Biochemistry 2019Quote: ... pET151/D-TOPO plasmids with the sequences of interest inserted under control of Lac operon and T7 viral promoter with N-terminal hexa-histidine tag and Ampicillin selection marker was obtained from Invitrogen. Sequences were optimized for expression in E ...
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bioRxiv - Molecular Biology 2019Quote: ... The sequence of RVFV IGR and flanking nucleotides of the N and NSs genes were synthesized by GeneArt (Thermo Fisher) for stepwise assembly of the complete minigenome in the pX12ΔT vector ...
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bioRxiv - Biophysics 2020Quote: A full-length cDNA encoding the methyltransferase SUV39H1 was amplified by PCR and fused with an N-terminal His6-MBP tag in pET-Duet1 (Invitrogen).
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bioRxiv - Neuroscience 2019Quote: ... a Tet-inducible EGFP-RBM45 construct was generated by inserting the EGFP-RBM45 (N-terminal EGFP fusion) into the pcDNA4/TO vector (Invitrogen). The sequences of all constructs were confirmed by Sanger DNA sequencing and the sizes of the expressed proteins confirmed by Western blot.
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bioRxiv - Molecular Biology 2019Quote: ... The VTN-N solution was removed prior to sticking the Press-to-Seal Silicone Isolator with Adhesive (Thermo Fisher Scientific) onto the nanosheets to control the density of the cells (see “Cell cultures” in METHODS).
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bioRxiv - Immunology 2021Quote: ... virus-infected cells were detected using an in-house rabbit polyclonal antibody specific for SARS-CoV-2 N/M/E followed by horseradish peroxidase-conjugated goat anti-mouse IgG (Invitrogen). TCID50 was calculated using the Reed-Muench method.
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bioRxiv - Cell Biology 2021Quote: ... full length N-terminally 3xFLAG tagged human KNSTRN ORF was cloned into pTREx-DEST30 vector by serial Gateway recombinations (Invitrogen) according to the manual ...
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bioRxiv - Cell Biology 2021Quote: ... TDP-43 CRISPR KO HeLa cells were lysed for N/C fractionation and SDS-PAGE with the NE-PER kit (ThermoFisher) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Live cells were imaged using an Olympus 40x UPlanFL N Oil NA1.3 objective in imaging medium (Leibovitz’s L-15 medium (Gibco™ 21083027) without phenol red ...
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bioRxiv - Systems Biology 2020Quote: ... the mouse mNCK2 cDNA sequence was cloned via restriction enzymes in frame with a N-terminal 3XFLAG tag in pcDNA3.1(-) (ThermoFisher Scientific) for western blot (WB ...
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bioRxiv - Biochemistry 2020Quote: ... were PCR-amplified and cloned into the pDEST15 vector encoding an N-terminus GST tag using the Gateway Cloning Technology (Invitrogen)27 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... the protein with an N-terminal His10 tag was expressed in Sf9 cells following the Bac-to-Bac baculovirus system (Invitrogen). Cells were collected and solubilized to extract protein in the buffer containing 25 mM MES pH 6.0 ...
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bioRxiv - Synthetic Biology 2021Quote: ... These two proteins were labeled at the cysteine using thiol-reactive BODIPY™ FL N-(2-Aminoethyl) Maleimide (Thermo Fisher). The proteins were mixed with 10-fold excess BODIPY™ FL N-(2-Aminoethyl ...
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bioRxiv - Microbiology 2020Quote: ... The plasmids were purified from 2 ml of the O/N culture using the GeneJET Plasmid Miniprep Kit (ThermoFisher Scientific), and sent for Sanger sequencing to DNA Sequencing and Genomics ...
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bioRxiv - Microbiology 2020Quote: PvRBP2b161-1454 and TfR1 were labelled with N-hydroxysuccinimide ester-activated DyLight 488 (DL488) and DyLight 594 (DL594) (Life Technologies) respectively ...
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bioRxiv - Cell Biology 2021Quote: ... C-term (aa 166-499)) were produced by PCR from the HA-PRPF31 plasmid and cloned into the pcDNA3.1 N-HA vector (Thermo Fisher). Deletion constructs of PRPF6 (NTD (aa 1-126) ...
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bioRxiv - Biophysics 2022Quote: The SARS-CoV-2 Omicron RBD and the N-terminal peptidase domain of ACE2 were expressed using the Bac-to-Bac baculovirus system (Invitrogen) as previously stated ...
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bioRxiv - Microbiology 2022Quote: SARS-CoV-2 Beta RBD for crystallization (residues 328-531 of S protein from GenBank NC_045512.2 with N-terminal signal peptide and C-terminal 8xHis-tag) was expressed in Expi293F (Thermo Fisher Scientific) cells in the presence of 10 µM kifunensine at 37°C and 8% CO2 ...
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bioRxiv - Neuroscience 2022Quote: ... The real-time quantitative PCR (qRT-PCR) was performed with TaqMan Fast Advanced Master mix (ThermoFisher Scientific, catalog n°4444963) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... a 6 x His tag and linker was fused to the N-terminus of the GJA1-20k sequence and cloned into the pET301/CT-DEST vector via Gateway cloning (Invitrogen). The primers used to amplify the sequence are (5’ to 3’ ...
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bioRxiv - Developmental Biology 2022Quote: Ear or tail biopsies were lysed overnight (o/n) at 55°C in DirectPCR lysis reagent (BioSite) in the presence of 0.1 mg/mL Proteinase K (Thermo Scientific). Lysis was stopped by heat inactivation (45min ...
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bioRxiv - Microbiology 2019Quote: Immunohistochemistry was performed using monoclonal antibodies directed to MV N protein (clone 83KKII, Chemicon [57]) or rabbit polyclonal antibody directed to GFP (Invitrogen). Goat anti-mouse IgG1 or goat anti-rabbit antibody conjugated with biotin was included as secondary antibody ...
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bioRxiv - Immunology 2020Quote: ... and SARS-CoV-2 and the N-terminal peptidase domain of human ACE2 (residues Ser19-Asp615) were expressed using the Bac-to-Bac baculovirus system (Invitrogen) as previously described 18,19,37-39 ...
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bioRxiv - Microbiology 2020Quote: Human IFITM proteins and SARS-CoV-2 viral proteins were cloned into MaMTH N-term tagged Prey and C-term tagged Bait vectors respectively using Gateway cloning technology (ThermoFisher). Correctness of recombined insertions was confirmed by Sanger sequencing (Eurofins) ...
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bioRxiv - Microbiology 2020Quote: Beads were labeled with the following N-hydroxysuccinimide ester (NHS)-activated fluorophores: (i) Alexa Fluor 488 NHS ester (Fisher Scientific) (ii ...