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Citations for Thermo Fisher :
1351 - 1400 of 10000+ citations for 6H Dibenzo b d pyran 3 pentanaminium 6a 7 10 10a tetrahydro 1 hydroxy N N N 6 6 9 hexamethyl 6aR trans 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: N-glycan samples were loaded on a porous graphitic carbon column (100 x 0.32 mm, Thermo Scientific) using 10 mM ammonium bicarbonate buffer as the aqueous solvent (solvent A ...
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bioRxiv - Developmental Biology 2024Quote: ... inserts were then subcloned into pCS2+ N HA tagged vectors that had been converted into Gateway (Invitrogen) destination vectors ...
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bioRxiv - Biochemistry 2024Quote: ... Digested peptides were eluted through centrifugation and labeled using commercially available TMTsixplex tags (ThermoFisher, P/N 90061). After labeling ...
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bioRxiv - Cancer Biology 2024Quote: ... N-glycosylation modification of proteins was assessed using the PNGase F Glycan Cleavage Kit (A39245, Gibco, USA). In brief ...
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bioRxiv - Molecular Biology 2023Quote: ... Then recombination step was done (Gateway® LR Clonase® II Enzyme mix, INVITROGEN, cat n°11791020) in the final mammalian cellular expression plasmid pEF-Dest51 (InvitrogenTM) ...
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bioRxiv - Biochemistry 2023Quote: ... N-terminally biotinylated 67B89 target protein was immobilized on Streptavidin Dynabeads M-270 (Invitrogen, Carlsbad, CA, USA). The target-coated beads were blocked with BSA and incubated with a suspension of 3.4·1012 phages in the presence of a 10-fold molar excess of the off-target (variant 6789) ...
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bioRxiv - Genomics 2023Quote: ... The homogenate was layered above 8mL of sucrose solution in ultracentrifuge tubes (Thermo Scientific, Cat N# 03699) (1mL per tube ...
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bioRxiv - Biochemistry 2022Quote: ... Target protein was coated overnight (O/N) at 4 °C on MaxiSorp™ 96-well plates (Nunc). ELISA-blocking buffer (PBS containing 0.5% (w/v ...
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bioRxiv - Cell Biology 2023Quote: A cDNA encoding p38α was subcloned into pcDNA6 with an N-terminal myc tag (Invitrogen, Carlsbad, CA), and a kinase-dead (D168A ...
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bioRxiv - Neuroscience 2023Quote: The resulting peptide mixture was resolved by liquid chromatography (LC) using an EASY n-LC (Thermo Scientific) UHPLC system with buffer A (2% v/v acetonitrile/0.1% v/v formic acid ...
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bioRxiv - Molecular Biology 2023Quote: ... Then recombination step was done (Gateway® LR Clonase® II Enzyme mix, INVITROGEN, cat n°11791020) in the final mammalian cellular expression plasmid pEF-Dest51 (InvitrogenTM) ...
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bioRxiv - Neuroscience 2023Quote: ... the pellet (synaptosomal fraction) was re-suspended in N-PER Neuronal Protein Extraction Reagent (ThermoFisher Scientific; 87792). The homogenate was transferred to an appropriate microcentrifuge tube and incubated on ice for 10 minutes ...
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bioRxiv - Microbiology 2023Quote: ... Vero E6-TMPRSS2 cells were fixed with 4% paraformaldehyde and stained with anti-N (Invitrogen; MA1-7403). The number of fluorescent foci was measured using a Cytation 5 Cell Imaging Multimode Reader (BioTeK).
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bioRxiv - Bioengineering 2023Quote: Bone explants (n=8) were cultured in medium consisting of α-MEM (41061, GibcoTM, Thermo Fisher Scientific), 10% v/v FBS (HycloneTM ...
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bioRxiv - Biochemistry 2023Quote: ... Digested peptides were eluted through centrifugation and labeled using commercially available TMTsixplex tags (ThermoFisher, P/N 90061). After labeling ...
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bioRxiv - Bioengineering 2024Quote: ... Unreacted maleimides were quenched by incubating the microgels in 100 mM N-acetyl-L-cysteine (Acros Organics) overnight at 37 °C ...
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bioRxiv - Cancer Biology 2024Quote: METTL3 siRNAs were used to induce transient knockdown in SK-N-BE(2) cells with RNAiMAX (ThermoFisher) reagent ...
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bioRxiv - Cancer Biology 2019Quote: ... 5(6)-carboxy-2’,7’-dichlorodihydrofluorescein diacetate (carboxy-H2DCFDA; CA-DCF-DA; (C400, ThermoFisher Scientific)) at a stock concentration of 20 mM in DMSO was diluted in DMEM without phenol red to a concentration of 40 μM ...
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bioRxiv - Cell Biology 2022Quote: ... and 7-AAD (7-Aminoactinomycin D) (Thermofisher, # A1310) was used for DNA staining ...
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bioRxiv - Cancer Biology 2022Quote: ... and 1 mM 7-Aminoactiomycin D (Thermo Fisher Scientific) and analysis by Flow Cytometry using a LSRFortessa X20 (BD Biosciences).
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bioRxiv - Cancer Biology 2021Quote: ... cells were marked with 2,3×10−3 μg/μL 4’,6-Diamidino-2-phenylindole dihydrochloride (DAPI, Thermo Fisher Scientific) for 10 minutes at room temperature in the dark ...
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bioRxiv - Molecular Biology 2021Quote: ... in a 1:9 ratio (in 6-well plates at 60-70% confluency) using Lipofectamine®LTX with Plus™ Reagent (Invitrogen) in Opti-MEM according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... labelled with the fluorescent lipid dye BODIPY® FL Maleimide [BODIPY® FL N-(2-Aminoethyl) Maleimide] (Thermo Fisher Scientific; 1:200) and incubated at room temperature in the dark 10 minutes prior to analysis ...
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bioRxiv - Immunology 2021Quote: ... to target the N1 amplicon of the N gene along with TaqPath 1-Step RT-qPCR Master Mix (Applied Biosystems Waltham, MA). For the subgenomic assay ...
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bioRxiv - Microbiology 2021Quote: ... sterols dissolved in chloroform were dried under nitrogen gas (N-EVAP, Organomation) followed by high vacuum for at least 1 hour (Savant SpeedVac, Thermo Fisher Scientific). They were then hydrated with 2.5 mM MβCD prepared in serum-free medium ...
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bioRxiv - Molecular Biology 2020Quote: ... linker oligos with a 5’-terminal amine modification were obtained from IDT (Coralville, Iowa) and activated using sulfo-SMCC (Sulfosuccinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate) reagent (Thermo Fisher, #A39268) according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... 30 and 60 min after induction of neutralization of Col 1 and enzymatic gelation (n=2-5 samples/group) of freshly prepared materials with a Multiskan™ GO Microplate Spectrometer (Thermo Scientific). The following solutions were analyzed ...
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bioRxiv - Microbiology 2021Quote: ... diluted 1:1000 in PBS for 15 m at RT and mounted on glass microscope slides (Fisher Scientific, cat n. 1157-2203) using VectaShield Vibrant mounting reagent (2B Scientific).
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bioRxiv - Cell Biology 2021Quote: ... co-cultures were fixed in 4% PFA for 30 min and immunostained for the anti-N (protocol described above) and with HCS Cell Mask TM Blue Stain (Invitrogen, 1:300). Images were acquired on an LSM 700 confocal microscope (Zeiss ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were washed twice with PBS and overlaid with 200 μL of alkylation buffer (40 mM HEPES, 50 mM NaCl, 1 mM EGTA, protease inhibitors) supplemented with 25 mM N-ethylmaleimide (NEM; Fisher Scientific, AA4052606). Cells were incubated for 10 minutes at room temperature and then 20 μL of 10% CHAPS detergent (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2022Quote: ... Subsamples of 1000–2000 μg were carefully weighed in tin capsules to determine the total N percentage (N% as mg (100 mg DW)−1) and the 15N abundance using a FLASH 2000 Organic Elemental Analyzer (Thermo Fisher Scientific) coupled to a Delta V Advantage isotope ratio mass spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: ... The Flag plasmids were transiently transfected in to N/Tert-1 cells using Lipofectamine™ RNAiMAX transfection (Invitrogen, catalog no. 13778-100) protocol and transfected in to U2OS cells using the calcium phosphate transfection protocol.
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bioRxiv - Developmental Biology 2023Quote: ... Two month old kidneys were harvested and isolated kidney cortex was cross-linked with 2 mM Di (N succinimidyl) glutarate (DSG, Proteo Chem C1104) in PBS for 30 min followed incubation in 1% formaldehyde (Thermo Scientific 28906) for an additional 10 min at room temperature and quenched with 125 mM glycine for 5 min ...
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bioRxiv - Microbiology 2021Quote: ... The freeze-dried material was dissolved in 300 μl of 6:1 (v/v) of methyl sulphoxide D6 (99.9% atom D + 1% tetramethylsilane, ACROS Organics):D2O (100% atom D ...
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bioRxiv - Cell Biology 2020Quote: Bone marrow cells were isolated and cultured for 7±9 d in RPMI-1640 medium (Gibco, ThermoFisher, Waltham, MA) supplemented with 10% low endotoxin defined FBS (Hyclone ...
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bioRxiv - Cell Biology 2020Quote: Bone marrow cells were isolated and cultured for 7±9 d in RPMI-1640 medium (Gibco, ThermoFisher, Waltham, MA) supplemented with 10% low endotoxin defined FBS (Hyclone ...
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DNA uptake by cell wall-deficient bacteria reveals a putative ancient macromolecule uptake mechanismbioRxiv - Microbiology 2022Quote: ... 10 mM Laurdan (6-Dodecanoyl-2-Dimethylaminonapthalene) stock solution (Invitrogen) was prepared in 100% dimethylformamide (DMF ...
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bioRxiv - Cancer Biology 2023Quote: ... selected using blasticidin (6 – 10 µg/ml, Thermo Fisher Scientific), and subjected to single-cell sorting ...
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bioRxiv - Cell Biology 2022Quote: ... DNP (EM 1:6-1:30, #71-3500, Invitrogen), DPP4 (IF/FACS 1:100 ...
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bioRxiv - Microbiology 2021Quote: ... using QuantStudio 6 or 3 Flex Real-Time PCR System (Applied Biosystems). SARS-CoV-2 standards with known copy numbers were used to construct a standard curve and calculate copy numbers/mL or copy numbers/g.
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bioRxiv - Microbiology 2023Quote: ... using QuantStudio 6 or 3 Flex Real-Time PCR System (Applied Biosystems). SARS-CoV-2 standards with known copy numbers were used to construct a standard curve and calculate copy numbers/mL or copy numbers/g ...
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bioRxiv - Pathology 2024Quote: ... Cells were subcultured at a 1:3 to 1:6 ratio using Gibco TrypLETM Express Enzyme (12605010, Fisher Scientific) for cell dissociation ...
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bioRxiv - Immunology 2024Quote: ... primary Rhinolophus ferrumequinum bat cells were seeded in 6-well plate at a density of 6×105 cells/well with DMEM containing 10% FBS (Gibco). After 12 h ...
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bioRxiv - Biochemistry 2019Quote: ... Sf9 cells were grown in 1X 10 6 for 1 litre of Sf900 II (Gibco) media in presence of antibiotic and antimycotic (Gibco ...
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bioRxiv - Systems Biology 2023Quote: Approximately 1*10^6 UWB1.289 were lysed in 650 µl Tri reagent (Thermo Fisher, #AM9738) and bead milled with the following settings ...
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bioRxiv - Neuroscience 2024Quote: ... 20 ng/mL Interleukin-6 (IL-6) (Thermo Fisher Scientific), 20 ng/mL Interleukin-3 (IL-3 ...
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bioRxiv - Cancer Biology 2023Quote: ... Surface CRT was detected using anti- CRT(N) or anti-CRT(Cmut) antibody (1:200 dilution) followed by staining with Alexa Fluor™ 647 conjugated secondary antibody (Invitrogen A21245, 1:2000 dilution). Total CRT in healthy donor platelets was stained with anti-CRT(N ...
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bioRxiv - Immunology 2020Quote: PBMC (1-2*10^6 cells) and pDC (1-4*10^4) were maintained for 16 h in RPMI1640+10% FBS+ 1% (PS) (Gibco Laboratories) in 96 well round bottom plates ...
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bioRxiv - Microbiology 2024Quote: ... ten-fold serial dilutions from 10-1 to 10-6 of media from infections were prepared in 1 x PBS (Gibco, #14190250). The media on Vero cells seeded the previous day was aspirated ...
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bioRxiv - Developmental Biology 2019Quote: ... pH=6 (ThermoFisher). Sections were then blocked for 1h at RT with 10% normal donkey serum (Chemicon International Inc ...