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Citations for Thermo Fisher :
1301 - 1350 of 10000+ citations for 6H Dibenzo b d pyran 3 pentanaminium 6a 7 10 10a tetrahydro 1 hydroxy N N N 6 6 9 hexamethyl 6aR trans 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2020Quote: ... Samples were then derivatized with 25 µL of N,O-bis (Trimethylsilyl)trifluoroacetamide with Trimethylchlorosilane (BSTFA with 1% TMCS, Thermo Scientific) for 1h at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: ... was used to generate amplified and biotinylated sense-strand cDNA from the entire expressed genome according to the GeneChip WT PLUS Reagent Kit User Manual (P/N 703174 Rev 1; Affymetrix Inc.). GeneChip HTA Arrays were hybridized for 16 h in a 45 °C oven ...
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bioRxiv - Microbiology 2022Quote: ... The levels of RNA corresponding to the N protein-encoding gene of SARS-CoV-2 were measured using the TaqMan Fast Virus 1-step Master Mix (Thermo Scientific). Each 20-μL reaction mixture contained 5.0 μL of 4× TaqMan Fast Virus 1-Step Master Mix ...
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bioRxiv - Systems Biology 2020Quote: ... cells were stained at 4°C o/n in 50 µL of the 1st antibody diluent (rabbit anti-ZO-1, 61-7300, Thermo Fisher Scientific Inc. ...
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bioRxiv - Biochemistry 2020Quote: ... or mouse monoclonal anti-N-Cadherin clone GC-4 followed by AlexaFluor 647-conjugated goat anti-mouse antibody (1:200 dilution, ThermoFisher Scientific). Staining was performed for 30 min at 4°C ...
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bioRxiv - Microbiology 2022Quote: Copies of the SARS-CoV-2 N gene (genomic) were measured by qRT-PCR TaqMan Fast Virus 1-step assay (Applied Biosystems). SARS-CoV-2 specific primers and probes from the 2019-nCoV RUO Assay kit (Integrated DNA Technologies ...
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bioRxiv - Cancer Biology 2023Quote: ... Abcam, USA) (Anti-E-cad/N-cad/Vimentin; 1:200, Cell Signalling, USA) (Rhodamine phalloidin dye, 6.6 µM, ThermoFisher Scientific, USA) at room temperature for 1 hour ...
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bioRxiv - Developmental Biology 2023Quote: Stem cells were grown on culture plates coated with 5micro μg ml-1 recombinant human Vitronectin (rhVTN-N, Life Technologies, #A14700). HESCs were grown in mTeSR1 (StemCell Technologies ...
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bioRxiv - Microbiology 2023Quote: ... prior to a one-hour incubation with SARS-CoV-2 N protein antibody (clone 1C7C7) at a 1:300 dilution and a AF594-conjugated Goat-anti-mouse secondary (ThermoFisher, A11005). Slides were counterstained with DAPI and scanned on an Akoya Polaris Vectra imaging system ...
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bioRxiv - Bioengineering 2023Quote: ... The levels of RNA corresponding to the N protein-encoding gene of SARS-CoV-2 were measured using the TaqMan Fast Virus 1-step Master Mix (Thermo Scientific). Each 20-μL reaction mixture contained 5.0 μL of 4× TaqMan Fast Virus 1-Step Master Mix ...
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bioRxiv - Microbiology 2023Quote: ... the membrane was incubated O/N at 4°C with gentle agitation with 1:2000 goat anti-human complement C3 antibody (Thermo Scientific) diluted in 1X TBS/0,5% Tween/3% BSA ...
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bioRxiv - Physiology 2023Quote: ... the tissue was stained with NeuroTrace™ green fluorescent Nissl stain (1:200 in PBS, Life Technologies, catalog No. N-21480) for 20 min at room temperature ...
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bioRxiv - Neuroscience 2023Quote: The forebrain sections were incubated with Blue NeuroTrace Fluorescent Nissl Stains (1:100 dilution by PBS, N-21479, Thermo Fisher Scientific) at room temperature for 2-3 hours ...
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bioRxiv - Biophysics 2024Quote: Vero cells (ATCC CCL-81) were cultured in 35 mm confocal dishes (D35C4-20-1-N) with DMEM (Gibco, Carlsbad, CA) supplemented with 5% FBS (Gibco ...
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bioRxiv - Molecular Biology 2022Quote: ... and modified pLX301 vectors 9 containing either a siRNA-resistant version of HA-NOL7 CDS or empty vector in a 1:9:10 ratio (1 μg:9 μg:10 μg for a 10-cm plate) with Lipofectamine 2000 (Life Technologies, 11668019) per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... a 7-9% agarose (Invitrogen, cat no. 16500500) pad made from the imaging media was gently placed over the top of the worms ...
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bioRxiv - Bioengineering 2021Quote: ... The composition of 1 L MM1 (pH = 6) was 10 g glucose (Acros Organics), 5 g of (NH4)2SO4 (Lach-Ner) ...
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bioRxiv - Cell Biology 2022Quote: ... For the bulk endocytosis experiments using the 4-[6-[4-(diethylamino)phenyl]-1,3,5-hexatrien-1-yl]-1-[3-(triethylammonio)propyl]-pyridiniumbromide dye (FM 4-64, Invitrogen), the cells were prepared as previously described [41] ...
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bioRxiv - Molecular Biology 2021Quote: ... [30] at a concentration of 57.5 μM (5% of the suggested concentration in RPMI 1640 medium) and media were supplemented with 10% dialyzed fetal bovine serum (Invitrogen, cat n°26400-044). Cell populations were cultured for at least six population doublings for complete incorporation of the labelled L-Arg.
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bioRxiv - Biophysics 2023Quote: Human neuroblastoma cell line (SK-N-BE) stored at -80°C in freezing medium (RPMI Gibco® supplemented with 20% FBS Gibco and 10% DMSO), were quickly defrosted at 37°C ...
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bioRxiv - Bioengineering 2022Quote: Untargeted lipidomics profiling (n = 3) was performed via high-resolution liquid chromatography with tandem mass spectrometry (Thermo Scientific QExactive Plus/HF Orbitrap HR-LC-MS/MS) at the Mass Spectrometry Core at Beth Israel Deaconess Medical Center in Boston ...
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bioRxiv - Cell Biology 2020Quote: ... Harvested equine explants were allowed to recover in 6-well plates for 3-7 days in the following control medium: DMEM/F12 medium (11330, Thermo Fisher Scientific, Waltham, MA), 10% fetal bovine serum (Seradigm 1500-500 ...
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bioRxiv - Cell Biology 2022Quote: ... 1:200 O/N at 4 °C) and Alexa Fluor 594-conjugated anti-mouse IgG antibody (#A11005, Invitrogen, 1:2000 for 2 hours RT). Nuclei staining was done in 0.5 μg/mL Hoechst 33324 (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... neural induction was initiated by changing the culture medium to a 1:1 mixture of DMEM/N-2 (DMEM/F-12 + GlutaMAX (Life Technologies, cat# 31331-028); 1X N-2 (Life Technologies ...
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bioRxiv - Cell Biology 2020Quote: ... 6 or 9 µl of 20 µM siRNA and 4 µl of Lipofectamine RNAi MAX (13778150, Invitrogen) were suspended in 100 µl of water (W4502 ...
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bioRxiv - Cell Biology 2022Quote: ... 6 or 9 μl of 20 μM siRNA and 4 μl of lipofectamine RNAi MAX (Invitrogen, 13778150) were suspended in 100 μl of water (Sigma ...
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bioRxiv - Biochemistry 2022Quote: ... The quality of obtained microsomes was tested with 9-amino-6-chloro-2-methoxyacridine (ACMA; Invitrogen A1324) fluorescence quenching assays.
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bioRxiv - Developmental Biology 2022Quote: ... bTSCs were passaged every 6 days at a 1:6 split ratio using Accutase (Gibco, A1110501). Each well of bTSCs was dissociated by 1 mL Accutase for 5 mins at 38.5 °C ...
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bioRxiv - Cancer Biology 2021Quote: SK-N-BE(2) and NB16 cells were seeded into 8-champer slides (Thermo Fisher Scientific, 154534) with a density of 6×103 cells/well overnight and treated with indicated treatment for 72h ...
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bioRxiv - Genomics 2020Quote: ... Nuclei were counterstained with DAPI and slides mounted with Fluoromount-G (Invitrogen, Cat. N: 00-4958-02).
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bioRxiv - Cell Biology 2020Quote: ... PAA gel surface activation reagents were ethyl(dimethylaminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) solution (Fisher Scientific). Collagenase from Clostridium histolyticum was purchased from Sigma.
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bioRxiv - Biochemistry 2020Quote: ... Rubisco holoenzyme was labeled at the N terminus with the fluorophore Alexa Fluor 532 NHS ester (ThermoFisher) according to manufacturer’s instructions (∼2 dye molecules bound per Rubisco holoenzyme) ...
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bioRxiv - Cancer Biology 2020Quote: ... MRC5-N cell line was grown in Minimum Essential Medium Eagle (MEM-aplha, Gibco Life Technologies 22561021), RPE-1 cell line was grown in Roswell Park Memorial Institute Media (RPMI ...
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bioRxiv - Genetics 2021Quote: Total RNA was extracted from tissues in adult flies (n=15 per genotype) using Trizol Reagent (Ambion). cDNA using oligo(dT ...
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bioRxiv - Biochemistry 2021Quote: ... 0.03% NP40) and proteins were eluted by incubation in 1x LDS-sample buffer (Thermofisher, c/n NP0007) at 65°C for 10 minutes ...
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bioRxiv - Molecular Biology 2019Quote: ... and PCR negative controls (two per PCR plate; n = 16) were MGW (Fisher Scientific UK Ltd, UK). PCR products were pooled to create sub-libraries (Fig ...
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bioRxiv - Plant Biology 2020Quote: ... Tissue C and N content was measured with an elemental analyzer (FlashEA 1112, Thermo Scientific, Walham, MA), and the percentage of C and N per sample was calculated with the instrument’s software (Eager Smart ...
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bioRxiv - Genetics 2019Quote: ... and CREB3L2-N or empty vector were mixed in various combinations and transfected using Lipofectamine 2000 (Invitrogen). Forty-eight hours later ...
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bioRxiv - Developmental Biology 2020Quote: ... was used to transfer the WNK1 sequence into the Vivid Colors pcDNA6.2/N-YFP vectors (Thermo Fisher), which created the mammalian expression vectors with YFP fused to the N-terminal end of WNK1 (Fig ...
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bioRxiv - Genomics 2021Quote: ... DNA quantification was performed using a Qubit™ 1X dsDNA HS Assay Kits (ThermoFisher, cat. n. Q33230), and DNA quality assessed with an Agilent 2100 Bioanalyzer.
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bioRxiv - Cancer Biology 2020Quote: ... MRC5-N cell line was grown in Minimum Essential Medium Eagle (MEM-aplha, Gibco Life Technologies 22561021), RPE-1 cell line was grown in Roswell Park Memorial Institute Media (RPMI ...
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bioRxiv - Genetics 2021Quote: ... 2 μl samples (in duplicates) were mixed with 100μl Infinity glucose reagent (Thermo Scientific, Cat. N. TR15421) in a 96-well plate ...
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bioRxiv - Genomics 2020Quote: ... Enzyme mix 2 was added and gently mixed using a disposable Pasteur pipette (ThermoFisher, Cat. N: PP89SA). Subsequently ...
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bioRxiv - Immunology 2021Quote: ... Gene expression assays were performed using Power SYBR® Green Master Mix (Cat. N. 4368577, ThermoFisher®) with primers described below ...
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bioRxiv - Cell Biology 2022Quote: ... Qiagen) in Optimem reduced serum media and lipofectamine (1μl/100 μl Optimem, RNAiMAX, Invitrogen, Cat. N. 56531) was added to the HUVEC and incubated overnight ...
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bioRxiv - Zoology 2023Quote: ... and 2-(N-morpholino)ethanesulfonic acid (MES) running buffer solution (Bolt™, Thermo Fisher Scientific, Waltham, Massachusetts). A standard ladder (Mark12™ ...
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bioRxiv - Molecular Biology 2022Quote: ... ROS content (n=60; minimum of 20 per group) with CellROX® Green Reagent (CRG) (Thermo Fisher) and MMP (n=60 ...
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bioRxiv - Molecular Biology 2022Quote: Plasmids encoding for c17orf80-BirA* N- or C-terminal fusions were generated using Gateway cloning technology (Invitrogen) using the c17orf80 sequence (NM_001100621 ...
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bioRxiv - Neuroscience 2024Quote: ... hiPS cells were cultured on six-well plates coated with recombinant human vitronectin (VTN–N, Gibco, A14700) in Essential 8 medium (Gibco ...
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bioRxiv - Cancer Biology 2024Quote: SK-N-BE(2) and NB16 cells were seeded into 8-champer slides (Thermo Fisher Scientific, 154534) with a density of 6×103 cells/well overnight and treated with indicated treatment for 72h ...