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Citations for Thermo Fisher :
1251 - 1300 of 10000+ citations for Dengue Virus Serotype 3 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... The cells were labeled with DiOC6(3) (Invitrogen, final concentration: 0.05 µg/ml) for 1 min at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... cells were passaged every 3-5 days using Accutase or ReleSR (ThermoFisher Scientific).
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bioRxiv - Cell Biology 2022Quote: ... cell lysates were run on NuPAGETM 3-8% Tris Acetate gels (Invitrogen EA0378BOX). To probe for CD63 ...
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bioRxiv - Genomics 2023Quote: ... cells were pre-cultured for 3 days in phenol red-free DMEM (Gibco) supplied with 5% dextran-coated charcoal (DCC ...
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bioRxiv - Cell Biology 2024Quote: ... and cell number/ml was counted in Countess 3 (Invitrogen by Thermofisher Scientific). Cell numbers were corrected for trypan blue addition and volume of resuspension and presented as histograms.
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bioRxiv - Cell Biology 2024Quote: ... and cell number/ml was counted in Countess 3 (Invitrogen by Thermofisher Scientific). Cell numbers were corrected for trypan blue addition and volume of resuspension and presented as histograms.
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bioRxiv - Cell Biology 2023Quote: ... INS-1 832/3 cells were transfected with 40 nM of Control (Ambion Silencer Select Negative Control #1 siRNA ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were passaged every 2-3 days using TryplLE™ express enzyme (GIBCO) and trypsin inhibitor (GIBCO).
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bioRxiv - Cancer Biology 2023Quote: ... and after an automatic counting by Countess 3 Automated Cell Counter (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... S10-3 cells were grown in Dulbecco’s Modified Eagle’s Medium (Gibco, Carlsbad, CA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2023Quote: ... Capan-2 and BxPC-3 cell lines were cultured in RPMI medium (Gibco) supplemented with 10% FBS (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... cells were washed 3 times with hepatocyte wash medium (Thermo Fisher Scientific, 17704024). Cells were then plated in a 6-well plate precoated with collagen I (50µg/mL ...
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bioRxiv - Cell Biology 2023Quote: ... cells were selected with 3 μg/ml puromycin (#A11138-03, Thermo Fisher Scientific). Single-cell clones that express similar TMEM192 levels were used in lyso-IP experiments.
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bioRxiv - Neuroscience 2024Quote: ... Cells were split every 3-4 days when confluent using TrypLE Express (Gibco). For all immunoblotting and imaging experiments ...
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bioRxiv - Microbiology 2024Quote: ... and total white blood cell count was determined on a Countess 3 (ThermoFisher) automated cell counter ...
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bioRxiv - Biochemistry 2024Quote: ... the cell density of each plate was determined using a Countess 3 (Thermofisher). The supernatant was discarded ...
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bioRxiv - Genomics 2024Quote: ... Cells were passaged every 2-3 days using 0.25% trypsin-EDTA (#25200056, Gibco) to keep them below 80% confluency ...
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bioRxiv - Cell Biology 2019Quote: ... Caspase-3/7 activation in live cells was monitored using CellEvent Caspase-3/7 Green Detection Reagent (Thermo Fisher Scientific, C10423, 1:1000) in IncuCyte Live Cell Analysis System ...
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bioRxiv - Microbiology 2020Quote: Vero E6 and Calu-3 cells (Calu-3:ATCC HTB-55; Vero E6: ATCC, CRL-1586) were maintained in high glucose DMEM (Gibco, Waltham, MA, USA) supplemented with 10% FBS (R&D Systems ...
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CRISPR screens for lipid regulators reveal a role for ER-bound SNX13 in lysosomal cholesterol exportbioRxiv - Cell Biology 2021Quote: ... cells were transfected with siRNAs targeting human SNX13 (5’- CAGAAAGGCUCAACAGAAAUU-3’) or SNX14 (5’-GGAUGAAAGUAUUGACAAAUU-3’) using Lipofectamine RNAiMax (Invitrogen, Cat#13778-075) according to the manufacturer ...
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bioRxiv - Microbiology 2022Quote: ... pTG-Luc and SARS2-COV2 spike expression vector were transfected into HEK 293T cells at the ratio of 3:4:3 using Lipofectamine 3000 transfection reagent (ThermoFisher Scientific, Waltham, MA). Accession IDs of the spike proteins used in the study were ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were incubated in 2 μM JC-1 (5, 5’, 6, 6’-tetrachloro-1, 1’, 3, 3’-tetraethylbenzimidazolylcarbocyanine iodide, Invitrogen, cat no: T3168) at 37°C for 15 min ...
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bioRxiv - Neuroscience 2022Quote: ... the medium was replaced every 2-3 days and cells passaged 1:2 or 1:3 weekly with 0.25% Trypsin/EDTA (Thermo Fisher Scientific, #25200-056) pre-warmed at 37°C.
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bioRxiv - Molecular Biology 2023Quote: ... Naïve hESCs were routinely passaged as single cells every 3 days at a ratio of 1:3 using TrypLE™ Express (1X) (Gibco, Thermo Fisher Scientific) and plated in fresh media supplemented with 10 μM of Y-27632 (Stemcell Technologies).
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bioRxiv - Cancer Biology 2024Quote: ... 48hrs after transfection the cells were fixed and stained for activated Caspase-3/7 using Apoptosis CellEvent™ Caspase-3/7 Green Detection Reagent (Thermo Fisher Scientific) according to manufacturers’ instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Established ovarian carcinoma cell lines OVCAR-3 (ATCC) and Kuramochi (JCRB Cell Bank) were cultured in RPMI (Invitrogen) supplemented with 10% fetal bovine serum (Gibco) ...
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bioRxiv - Microbiology 2021Quote: ... were seeded with PD07i epithelial bladder cells at a concentration of ∼ 3×106 cells in EpiLife Medium (Gibco) supplemented with growth supplements and antibiotics (HKGS and 100µg ml-1 Pen/Strep) ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were passaged 1:3 when they reached 80% confluence by harvesting cells with 0.25% trypsin EDTA (Gibco).
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bioRxiv - Microbiology 2019Quote: ... Bacterial cells were lysed by suspending cells in 3 μL of 100 mg/ml RNase A (Ambion, AM2286) and ten μL of 100 mg/ml lysozyme (Sigma ...
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CD56/NCAM mediates cell migration of human NK cells by promoting integrin-mediated adhesion turnoverbioRxiv - Cell Biology 2023Quote: Cell lysates from 3 × 106 cells were generated using RIPA Lysis and Extraction Buffer (Thermo Fisher Scientific; 89901) supplemented with 1X Halt protease inhibitor cocktail (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... and 4.5 µl of RfxCas13d protein (3 mg/ml) were mixed in 1 µl of nuclease-free water with phenol red (Invitrogen). One to 5 nL of RNPs were injected into one- to two-cell stage Ancistrus sp ...
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bioRxiv - Molecular Biology 2020Quote: ... The insoluble material was removed by centrifugation and the protein content in the supernatant was measured using a Qubit 3 fluorometer (Invitrogen) and equal amounts of proteins were separated in SDS-10 % polyacrylamide gels and blotted onto a nitrocellulose membrane (Amersham) ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates were then homogenized through a 26-gauge syringe 3 times and protein content measured by BCA (ThermoFisher; Waltham, MA). Samples were then diluted in 4X Sample Buffer (2% SDS ...
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Endothelial transmigration hotspots limit vascular leakage through heterogeneous expression of ICAM1bioRxiv - Immunology 2022Quote: ... Samples were boiled at 95ᵒC for 3 minutes to denature proteins and separated on a 4-12% NuPage Bis-Tris gel (Invitrogen, NP0322BOX). Proteins were transferred using an iBlot Gel Transfer device (Invitrogen ...
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bioRxiv - Genomics 2020Quote: ... Chromatin-antibody complexes were captured for at least 3 hr using a mix of Protein A and G Dynabeads (Invitrogen). The captured immunoprecipitated complex were washed 10 min each with sonication buffer (50 mM HEPES ...
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bioRxiv - Molecular Biology 2019Quote: ... Antibodies were added for 3 h before 45 min incubation with protein A or G beads (Dynabeads 10002D, 10003D from Thermofisher scientific ...
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bioRxiv - Genomics 2019Quote: ... The samples were cooled down to RT before loading them on a 3-8% Tris-Acetate Protein Gels (Invitrogen EA0375PK2). Next ...
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bioRxiv - Physiology 2021Quote: ... Crosslinking of Y-3 antibody and Protein A+G was done using sulfosuccinimidyl (4-iodoacetyl)aminobenzoate (Sulfo-SIAB, ThermoFisher 22327). For 1 mL of protein A+G agarose (ThermoFisher/Pierce #20421) ...
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bioRxiv - Genetics 2020Quote: ... samples were diluted in Laemmli sample buffer and 2 μg of protein was separated on a NuPage 3–8% Tris-Acetate gel (Invitrogen). The gel was then stained with Coomassie Blue and digitalized.
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bioRxiv - Biochemistry 2020Quote: ... The beads were washed 3 times then subjected for protein elution with 50 units of AcTEV protease (Invitrogen, 12575-015) in PBS buffer by 8 hr incubation in 4 °C on a rotator.
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bioRxiv - Biochemistry 2020Quote: ... Proteins were resolved on NuPage 3-8% w/v Tris-Acetate or 4-12% w/v Bis-Tris gels (Invitrogen) and transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Neuroscience 2020Quote: A total of 50 μg of protein for each of the samples was loaded into a pre-cast NativePAGE 3-12% Bis-Tris gel (Invitrogen). For the run ...
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bioRxiv - Neuroscience 2020Quote: ... Homogenates were submitted to an SDS-PAGE protein separation in 4-12 % or 3-8 % (depending on the molecular weight of the protein of interest) NuPAGE bis-tris gels (Invitrogen) and transferred to a nitrocellulose membrane (Amersham) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and 100 ng of yellow fluorescent protein (YFP) cDNAs in each well using 1:3 DNA: Lipofectamine 2000 (11668019, Invitrogen) ratio ...
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bioRxiv - Microbiology 2021Quote: ... Midguts were sonicated for 3 cycles of 15 seconds yielding approximately 100μg of protein from each pool as measured by BCA assay (Thermo Scientific). Samples were reduced with 5mM DTT for 30 mins at 50°C then alkylated with 15mM IAA for 30 mins at RT ...
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bioRxiv - Cell Biology 2021Quote: ... Eluted proteins were briefly subjected to SDS-PAGE (3 min at 200 V, 4– 12% Bis-Tris gel, Life Technologies) and stained with InstantBlue Coomassie protein stain before being washed with ddH2O overnight at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... incubated for 3 minutes at 95°C and loaded on NuPAGE 4 - 12 % Bis-Tris Mini Protein Gel (ThermoFisher Scientific). Proteins were separated by electrophoresis in NuPAGE MOPS SDS running buffer and transferred in Bjerrum Schafer-Nielsen transfer buffer (48 mM Tris ...
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bioRxiv - Developmental Biology 2021Quote: ... 20 μg protein lysates were subjected to gel electrophoresis on a 3-8% NuPAGE® Tris-Acetate gel (Life Technologies), and protein was transferred to a Immobilon-FL membrane (Millipore) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and 1X glyceraldehyde 3-phosphate dehydrogenase (GAPDH) or ribosomal protein large P0 (RPLP0) endogenous transcripts (Thermo Fisher Scientific; 4310884E; 4310879E) and 1X TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2019Quote: ... 10 or 15μg of protein was loaded per well on Tris-Acetate 3-8% gels with Tris-Acetate running buffer (NuPAGE, ThermoFisher) for detecting KIF26B protein or on 4-12% NuPAGE Bis-Tris Gel with NuPAGE MES SDS Running Buffer (NuPAGE ...