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Citations for Thermo Fisher :
1401 - 1450 of 10000+ citations for Dengue Virus Serotype 2 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... cDNA was synthesized from 1 μg total RNA using Moloney murine leukemia virus reverse transcriptase (Invitrogen) with oligo(dT ...
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bioRxiv - Microbiology 2020Quote: ... Five μL RNA was tested with TaqMan™ Fast Virus One-Step Master Mix (Applied Biosystems) using QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Biochemistry 2020Quote: ... RNA was reverse transcribed using Moloney Murine Leukemia Virus (M-MuLV) reverse transcriptase (Thermo Fisher Scientific), in the presence of oligo (dT)15 primers ...
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bioRxiv - Genomics 2021Quote: ... we reprogrammed fibroblast samples from 139 iPSCORE individuals using non-integrative Cytotune Sendai virus (Life Technologies) and the 149 iPSCs (7 subjects had 2 or more clones each ...
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bioRxiv - Immunology 2021Quote: ... RT-qPCR was performed using Taqman Fast Virus 1-step Master Mix (Thermo Fisher Scientific, 4444434) in StepOne Plus real-time PCR thermocycler (Applied Biosystems ...
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bioRxiv - Microbiology 2021Quote: RNA was extracted from 100 µl of virus stock using a KingFisher Flex (Thermo Fisher Scientific) in combination with the MagMax Viral Pathogen kit II (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2022Quote: ... vRNA was then reverse transcribed using the TaqMan Fast Virus 1-Step qRT-PCR kit (Invitrogen) and quantified on a LightCycler 480 (Roche ...
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bioRxiv - Microbiology 2022Quote: RNA was extracted from 100 μl of virus stock using a KingFisher Flex (Thermo Fisher Scientific) in combination with the MagMax Viral Pathogen kit II (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: Fibroblasts were reprogrammed using the Sendai virus reprogramming method as recommended by the manufacturer (Life Technologies). Briefly ...
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bioRxiv - Microbiology 2023Quote: ... tissue sections were incubated with the primary antibody (rabbit polyclonal anti-Influenza A virus NP [ThermoFisher Scientific ...
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bioRxiv - Genomics 2023Quote: ... Real-time PCR was conducted using TaqMan Fast Virus 1-Step Master Mix from Applied Biosystems in 384-well plates ...
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bioRxiv - Microbiology 2024Quote: ... cDNA of the virus genome was generated by reverse transcription (Verso cDNA Synthesis Kit, ThermoFisher Scientific) using MBTuni-12 (5′-ACGCGTGATCAGCAAAAGCAGG ...
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bioRxiv - Cell Biology 2020Quote: ... 1000 μg of total protein was incubated overnight at 4 °C with 2 μg α-GFP (Life Technologies/ThermoFisher Scientific ...
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bioRxiv - Immunology 2020Quote: IL-2/Fc fusion proteins were expressed using the Expi293 expression system according to manufacturer instructions (Thermo Scientific). Proteins were as human IgG1 Fc fused at the N-or C-terminus to human IL-2 through a (G4S)4 linker ...
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bioRxiv - Biochemistry 2022Quote: ... All proteins were expressed in 1-2 liters of SF900 III SFM media (Thermo Fisher Scientific, Waltham MA) set at 7 × 105 cells/ml in 2.8L or 5L Optimum Growth Flasks (Thomson Instrument Company Oceanside ...
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bioRxiv - Molecular Biology 2020Quote: ... Immulon 2 HB plates were coated with 2μg/ml Pierce recombinant protein A/G (ThermoFisher catalog no: 77677) or purified SARS-CoV-2 receptor binding domain (provided by Florian Krammer ...
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bioRxiv - Immunology 2021Quote: ... Biotinylated spike protein was purified using a 40K MWCO 2 mL Pierce Zeba™ desalting column (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2019Quote: ... and 2 µl of RNAPII S5P (61085, Actif motif) and 20 µl Protein-G-Dyna beads (Life Technologies) were used per ChIP ...
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bioRxiv - Molecular Biology 2019Quote: siRNA mediated protein depletion of ESRP1/2 was carried out using Lipofectamine RNAiMAX Transfection Reagent (Thermo Fisher, 13778075) as per the manufacturer’s instructions and for the times indicated ...
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bioRxiv - Cell Biology 2021Quote: ... purified protein was dialyzed overnight against ddH2O using 2 mL Slide-A-Lyzer 10K MWCO tubes (Thermo Scientific). Protein concentration was determined using the Micro BCA Protein Assay Kit (Thermo Scientific) ...
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bioRxiv - Biochemistry 2021Quote: ... 2 μL protein solutions (0.1 mg/mL) and 18 μL of buffer containing 10X of Sypro Orange (Invitrogen) were added to the wells of a 96-well ...
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bioRxiv - Biochemistry 2021Quote: The gene encoding the PLpro (also known as nsp3) protein from SARS-CoV-2 (YP_009725299.1) was also synthesized commercially (Invitrogen) and cloned into the pSUMO1 vector with N-terminal 8xHis conjugated with yeast SUMO ...
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bioRxiv - Systems Biology 2020Quote: ... 0.024 μg/mL of LIF (EMBL Protein expression and purification core facility) and 0.12mM of 2-Mercaptoethanol (Gibco). Cells were maintained on MEFs and incubated at 37°C with 5% CO2 and 95% relative humidity ...
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bioRxiv - Microbiology 2022Quote: ... Western blots were performed on the immunoprecipitated protein samples using the mouse-anti-HA tag (2-2.2.14) primary antibody (Invitrogen) (1:1,000 ...
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bioRxiv - Microbiology 2022Quote: ... Fifty μL of 1:3000 diluted mouse anti-SARS-CoV-2 Spike Protein S1 monoclonal antibody (GT623, Invitrogen) in 1% BSA-PBS blocking buffer was added and incubated at room temperature for 30 min ...
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bioRxiv - Immunology 2020Quote: ... 2 mg of concentrated nanoparticles were labeled using an Alexa Fluor™ 647 Protein Labeling Kit (Thermo Fisher). The final degree of labeling (DoL ...
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bioRxiv - Developmental Biology 2019Quote: ... and the rest was immunoprecipitated at 4°C for 2 h using Dynabeads Protein A (Life Technologies, # 10008D)-conjugated anti-m6A antibody (Synaptic systems ...
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bioRxiv - Immunology 2020Quote: ... Protein was subsequently transferred to a nitrocellulose membrane using the iBlot 2 Dry Blotting System (Thermo Fisher Scientific). Following transfer ...
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bioRxiv - Plant Biology 2020Quote: ... Total proteins were extracted from infiltrated leaf patches in 1 ml 2×NuPAGE LDS sample buffer (Invitrogen, NP0008) containing 0.05mL/mL β-mercaptoethanol ...
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bioRxiv - Immunology 2020Quote: ... Protein samples (2 µg) were mixed with Bolt™LDS sampling buffer and sample reducing agent (Life Technologies), and heated at 70°C for 5 min before separation on 4-12% Bis-Tris Plus ready gel (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2021Quote: ... goat anti-Synaptopodin 2 µg) or control IgG coupled to Protein G-coupled magnetic beads (Dynabeads, Life Technologies). Unbound material was removed and the beads washed three times with homogenization buffer ...
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bioRxiv - Immunology 2020Quote: ... Anti-SARS-CoV-2 Coronavirus Spike protein (subunit 1) polyclonal antibody was purchased from Invitrogen (Carlsbad, CA, USA). Anti-SARS-CoV-2 Spike RBD rabbit polyclonal antibody was purchased from SinoBiological (Beijing ...
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bioRxiv - Immunology 2021Quote: ... a 96-well plate was loaded with mixtures of 2 μL 100X SYPRO Orange protein gel stain (Invitrogen) and 18 μL target protein at 15 μM in HBS-EP buffer (10 mM HEPES ...
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bioRxiv - Microbiology 2021Quote: ... and anti-SARS-CoV-2 spike protein (RBD) polyclonal antibody (RBD, 1:1,000, Invitrogen cat. no. PA5-114451). All primary antibodies were diluted in PBST + 5% milk and applied to membranes for ≥16 hours at 4 °C ...
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bioRxiv - Immunology 2022Quote: Codon-optimized SARS-CoV-2 spike protein expression plasmids were generated by cloning of gene fragments (Thermo Fisher) and/or site-directed mutagenesis (Q5 Site-Directed Mutagenesis Kit ...
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bioRxiv - Neuroscience 2022Quote: Immunofluorescence was performed using glial fibrillary acidic protein (GFAP) and 4′,6-diamidino-2-phenylindole (DAPI) solution (ThermoFisher). GFAP primary and secondaries were diluted to a ratio of 1:1000 and 5:1000 ...
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bioRxiv - Developmental Biology 2022Quote: ... P15 or adult mice (2 months) were first quantified using a Pierce BCA Protein Assay Kit (Thermofisher; 23225) with absorbance read using a Glomax Explorer Microplate reader (Promega ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were visualized using the Thermo Scientific Pierce ECL 2 Western Blotting Substrate (Thermo Fisher Scientific, Cat. 80196) or the SuperSignal West Femto Chemiluminescent Substrate (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... Expression of GOI protein (Figure 2) was determined by SDS–PAGE using 4–12% gradient polyacrylamide gels (Invitrogen) and Coomassie staining and by Western blotting using antigen-specific sera.
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bioRxiv - Biochemistry 2022Quote: ... 2-3 µL of 5 µM protein solution was introduced directly into Q-Exactive UHMR mass spectrometer (ThermoFisher) through gold coated capillary needles that were prepared in-house30 ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were migrated for 2-3 h at 120 V in NuPAGE MOPS SDS running buffer (#NP0001, Invitrogen) and transferred onto a PVDF membrane (#1704156 ...
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bioRxiv - Biochemistry 2023Quote: ... The eluates were subjected to 2-dimensional gel electrophoresis separation and the protein spots (Sypro Ruby, Molecular Probes) that were specifically present in the PNKP IP and not in the IgG IP were subjected to mass spectroscopic identification in the University of Texas Medical Branch Biomolecular Resource Facility.
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bioRxiv - Cell Biology 2023Quote: ... Protein from S and P fractions were mixed with 2 µL 0.5 M DTT and 4x LDS (Invitrogen), incubated at 70°C for 10 minutes and run on NuPAGE 10% Bis-Tris gels in MES/SDS-PAGE running buffer (1M MES ...
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bioRxiv - Immunology 2024Quote: ... or BA.5 (2 μg/mL) spike proteins were coated onto 96-well Maxisorp plates (ThermoFisher, Cat. #439454) in 50 μL of 50 mM Na2CO3 pH 9.6 overnight at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... or 2 μg of Flag Tag antibody (mouse) conjugated with Dynabeads Protein G Magnetic Beads (Invitrogen, 10004D, USA), or anti-HA magnetic Beads (Abmart ...
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bioRxiv - Cancer Biology 2024Quote: ... or with Protein A agarose beads incubated with 2 μg anti-LONP1 antibody (ThermoFisher Scientific 15440-1-AP) overnight (16 hours ...
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bioRxiv - Biophysics 2021Quote: A commercial HEK cell suspension cell line optimized for protein expression Expi293-F (Cat. No. A14635, Gibco Life Technologies ...
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bioRxiv - Genetics 2019Quote: ... Cell surface proteins were labeled with cell membrane impermeable Sulfo-NHS-Biotin (#21217, Thermo Fisher, Waltham, MA), quenched with 100 mM glycine ...
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bioRxiv - Biochemistry 2020Quote: ... The SALM3 fusion protein expression in transfected cell was detected with Floid™ Cell Imaging system (ThermoFisher) using immunofluorescence.
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bioRxiv - Immunology 2020Quote: ... Lung CD4+ T cells were stimulated ex vivo with cell stimulation cocktail containing protein transport inhibitors (ThermoFisher) for 4 h at 37° C prior to staining ...