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Citations for Thermo Fisher :
1251 - 1300 of 10000+ citations for Dengue Virus Serotype 2 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: Cells were lysed in Mammalian Protein Extraction Reagent (MPER; Thermo Scientific), supplemented with Halt protease inhibitor and Halt phosphatase inhibitor (Thermo Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant CEACAM-His proteins were expressed in Expi293F cells (Life Technologies) and purified by affinity chromatography (ÄKTA Pure ...
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bioRxiv - Immunology 2021Quote: ... Protein was produced by transfection into Expi293 cells (Thermo Fisher Scientific) according to standard manufacturer protocols ...
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bioRxiv - Immunology 2021Quote: ... Protein was produced by transfection into Expi293 cells (Thermo Fisher Scientific) according to standard manufacturer protocols ...
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bioRxiv - Immunology 2021Quote: ... Protein was produced by transfection into Expi293 cells (Thermo Fisher Scientific) in 96-well plate format ...
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bioRxiv - Immunology 2020Quote: ... Recombinant RBD proteins were produced in transfected FreeStyle 293F cells (Invitrogen) and purified by nickel affinity chromatography ...
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bioRxiv - Cell Biology 2021Quote: ... Protein constructs were expressed in HeLa cells from pcDNA 3.1+ (Invitrogen) plasmids by transient transfection using the TransIT®-LT1 Transfection Reagent (Mirus) ...
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bioRxiv - Biochemistry 2021Quote: All the proteins constructs were expressed in BL21(DE3) cells (Invitrogen), grown in Terrific Broth medium at 37°C until the OD600 reached a value of 1.5 - 2 ...
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bioRxiv - Cancer Biology 2020Quote: Proteins were extracted from cells or tissues using PARIS kit (Invitrogen). The protein concentration was determined using Pierce Rapid Gold BCA Protein Assay (Thermo Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... Cell total protein level was assessed by intracellular FITC (Fisher scientific) staining ...
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bioRxiv - Immunology 2021Quote: ... Recombinant proteins were produced using the Expi293F cells (Thermo Fisher Scientific) by transfecting 200×106 of these cells with purified DNA using the ExpiFectamine 293 Transfection Kit (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2020Quote: ... proteins were treated with a fixable blue dead cell stain (Invitrogen) and after washing ...
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bioRxiv - Biochemistry 2022Quote: Proteins were expressed in Escherichia coli BL21(DE3) cells (Life Technologies). Full-length and ΔAF-1 PPARγ proteins were expressed using an autoinduction procedure where cells were grown for 6 hrs at 37 °C followed by addition of 0.1 mM zinc chloride ...
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bioRxiv - Physiology 2022Quote: Cell and tissue lysates were prepared in Protein Extraction Reagent (ThermoFisher) supplemented with Halt Protease and Phosphatase Inhibitor Cocktail (ThermoFisher) ...
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bioRxiv - Cancer Biology 2022Quote: Subcellular protein fractionation kit for culture cells from Thermo Scientific (78840) was used to separate protein to cytosol ...
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bioRxiv - Molecular Biology 2023Quote: ... for protein transfer (X-cell-II blot module, Thermo Fisher Scientific). NC membrane was incubated in 5% skimmed milk ...
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bioRxiv - Genetics 2023Quote: ... cell protein lysates were harvested using a RIPA buffer (ThermoFisher Scientific) supplemented with Halt Protease & Phosphatase Inhibitor Cocktail (ThermoFisher Scientific) ...
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bioRxiv - Immunology 2023Quote: HA proteins were transiently expressed in HEK 293F cells (Thermo Fisher) at a density of 1.0 x 106 cells/mL with a 1:3 ratio of DNA to PEIMax ...
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bioRxiv - Neuroscience 2023Quote: ... The cell lysates were pre-cleaned with Dynabeads Protein G (Invitrogen) to avoid non-specific binding ...
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bioRxiv - Synthetic Biology 2023Quote: All proteins were expressed in Expi293F cells (Thermo Fisher Scientific, A14527). Proteins containing a biotinylation tag (AviTag ...
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bioRxiv - Developmental Biology 2023Quote: ... Cell lysates were incubated with protein A-Sepharose beads (Thermo scientific) for 1 hr at 4°C to eliminate nonspecific binding proteins ...
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bioRxiv - Molecular Biology 2024Quote: ... protein expression was carried out in HighFive cells (Thermo Fisher Scientific) at 130 rpm and 27 °C for 48 h ...
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bioRxiv - Synthetic Biology 2023Quote: All recombinant proteins were produced in Expi293F cells (Thermo Fisher Scientific) by transient transfection ...
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bioRxiv - Cancer Biology 2021Quote: ... of Iowa) was prepared by diluting virus stocks to 400 p.f.u./µL into OptiMEM (Gibco) followed by addition of CaCl2 to a final concentration of 10 µM ...
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bioRxiv - Microbiology 2020Quote: ... qPCR was performed using the TaqMan™ Fast Virus 1-Step Master Mix (Applied Biosystems). Assays were conducted on a QuantStudio5 Q-PCR machine (Applied Biosystems) ...
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bioRxiv - Molecular Biology 2021Quote: ... The assay was performed using the TaqMan® Fast Virus 1-Step Kit (Life Technologies) and kit manual ...
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bioRxiv - Molecular Biology 2020Quote: ... a 20μL monoplex reaction contained 5μL of 4x TaqMan Fast Virus Master Mix (Thermo Fisher), 0.6µL of each primer (0.3μM final concentration each) ...
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bioRxiv - Immunology 2019Quote: ... two-fold virus stock dilutions were prepared in U-shaped 96-well plates (Thermo Scientific). The same volume of a 1% human O+ red blood cell suspension (Innovative Research ...
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bioRxiv - Microbiology 2019Quote: ... Other antibodies were obtained from commercial vendors as follows: Anti-coxsackie virus B3 antibodies (ThermoFisher), Anti-adenovirus A5 antibodies (ThermoFisher) ...
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bioRxiv - Microbiology 2021Quote: ... and the number of virus particles was determined using a Nanodrop 2000 (Thermo Fisher Scientific). The adenovirus stocks were aliquoted and stored at -80°C.
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bioRxiv - Neuroscience 2021Quote: ... using TaqMan Fast Virus 1-Step Master Mix (PN4453800, Applied biosystems, Thermo Fisher Scientific, USA). PrimerTime primers and FAM-dye coupled detection probes were used for detecting Clstn3 mRNA level ...
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bioRxiv - Neuroscience 2021Quote: ... The final buffer for the virus was: dPBS + 5% Glycerol + 0.001 Pluronic F-68 (Invitrogen). The AAV titre (1.71 × 1013 GC/ml ...
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bioRxiv - Microbiology 2021Quote: ... Five μL RNA was tested with TaqManTM Fast Virus One-Step Master Mix (Applied Biosystems) using QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Neuroscience 2019Quote: ... The qPCR for Sendai virus detection was performed using TaqMan Gene Expression Assays (Life Technologies). Quantitative RT-PCR was performed using SYBR® Green PCR Master Mix (Applied Biosystems™ ...
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bioRxiv - Biochemistry 2022Quote: ... 1 μL of purified virus was analyzed with the Quant-it PicoGreen dsDNA Assay (Invitrogen).
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bioRxiv - Biochemistry 2022Quote: ... concentrated virus was run on a 4-20% Tris-Gly SDS-PAGE gel (Life Technologies), which was then analyzed using the Pierce Silver Stain Kit (Pierce ...
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bioRxiv - Immunology 2022Quote: ... For these assays we used TaqMan Fast Virus 1- Step Master Mix (ThermoFisher, catalog # 4444432) and custom primers/probes targeting the E gene sgRNA (forward primer ...
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bioRxiv - Molecular Biology 2022Quote: Viral RNA was extracted from amplified virus stock using Trizol reagent (Invitrogen; Cat no. #15596018). Reverse transcription was carried out using in-house produced Moloney murine leukemia virus (MMLV ...
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bioRxiv - Neuroscience 2021Quote: ... using TaqMan Fast Virus 1-Step Master Mix (PN4453800, Applied biosystems, Thermo Fisher Scientific, USA). PrimerTime primers and FAM-dye coupled detection probes were used for detecting Clstn3 mRNA level ...
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bioRxiv - Plant Biology 2021Quote: ... First-strand cDNA synthesis was performed using Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV, Invitrogen). PCR was performed in an AriaMx thermal cycler (Agilent ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... UKEi1-A was reprogrammed with a Sendai Virus strategy (CytoTune iPS Sendai Reprogramming Kit, ThermoFisher). These studies were approved by the Ethical Committee of the University Medical Center Hamburg-Eppendorf (Az ...
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bioRxiv - Microbiology 2019Quote: ... genomic DNA was extracted from 1010 virus using the PureLinkTM Genomic DNA mini kit (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... The virus was dialyzed with PBS supplemented with 0.05% of Pluronic F68 (Thermo Fisher Scientific) using 100-kDa filters (Millipore) ...
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Cholesterol deprivation induces TGFβ signaling to promote basal differentiation in pancreatic cancerbioRxiv - Cancer Biology 2019Quote: ... RNA was reverse transcribed (RT) using Moloney murine leukemia virus (MMLV) reverse transcriptase (#28025013, Ambion) and a mixture of anchored oligo-dT and random decamers (IDT) ...
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bioRxiv - Plant Biology 2019Quote: ... 10 IU of Moloney murine leukemia virus RNase H+ reverse transcriptase solution (Thermo Fisher Scientific), and appropriate buffer containing deoxynucleoside triphosphates (dNTPs ...
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bioRxiv - Cell Biology 2020Quote: ... Isolated total RNA was reverse transcribed with Moloney murine leukemia virus (MMLV) reverse transcriptase (Invitrogen) and oligo-dT primers ...
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bioRxiv - Microbiology 2021Quote: ... MRC-University of Glasgow Centre for Virus Research) were maintained in Leibovitz’s L15 media (Gibco) containing 10% FBS (Gibco) ...
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bioRxiv - Microbiology 2020Quote: Compound dilutions for virus treatment were performed in Minimum Essential Media (MEM) purchased by Gibco.
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bioRxiv - Microbiology 2019Quote: Concentrated virus aliquots were mixed with DiI (final concentration of 1 μM, Thermo Fisher, USA), vortexed and incubated for 1.5 h at room temperature (RT) ...
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bioRxiv - Immunology 2021Quote: ... RT-qPCR reactions were performed using TaqMan® Fast Virus 1-Step Master Mix (ThermoFisher) and 5 µl of isolated RNA as a template to detect a 132 bp sequence in the ORF1b/NSP14 ...