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Citations for Thermo Fisher :
1351 - 1400 of 10000+ citations for Dengue Virus Serotype 2 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: single-cell suspensions of spleen were prepared in FACS buffer (2% FBS, 2 mM EDTA, in PBS (Gibco)) and were treated with ACK buffer (Gibco ...
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bioRxiv - Immunology 2023Quote: ... cells treated with LP were loaded with 2 μM of the acetoxymethyl ester form of Fura-2 (Invitrogen) in RPMI-1640 at 37°C for 30 min and fluorescence data were analyzed using MetaFluor (Molecular Devices ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were imaged in M199 cell culture medium with 2% FBS (Thermo Fisher Scientific, 12340030) supplemented for 18 hours through the experiment with low glucose (5.5 mM) ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were then filtered through a 40-micron cell strainer (Thermo Fisher # 08-771-2) into a 50mL conical ...
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bioRxiv - Biophysics 2022Quote: ... cells were transfected at a density of ∼2 ×105 cells using Lipofectamine 2000 (Thermo Fisher) with 0.5-1 μg/μL of plasmid DNA containing Sec61-mEmerald ...
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bioRxiv - Cancer Biology 2023Quote: The T-cell engager (UCHT-2-03) was cloned and expressed using ExpiCHO cells (Invitrogen), and the recombinant protein was purified using Ni-column and size-exclusion chromatography (SEC).
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bioRxiv - Cancer Biology 2021Quote: The cell membrane protein samples were collected using Mem-PER eukaryotic membrane protein extraction reagent kit (Thermo Scientific, 89826, MA) according to the protocol instruction ...
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bioRxiv - Cancer Biology 2021Quote: Subcellular protein fractionation was performed either by using Subcellular Protein Fractionation Kit for Cultured Cells (Thermo Scientific; Cat. no. 78840) according to the manufacturer protocol or following the protocol below.
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bioRxiv - Cell Biology 2019Quote: Cells were lyzed using RIPA buffer and proteins were quantified using the BCA method (Protein BCA Assay Kit, Thermo Fisher). The protein extracts were separeted on 12% SDS-PAGE gels (50 μg of total proteins were loaded per well ...
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bioRxiv - Developmental Biology 2020Quote: Protein in testes and cultured cells were extracted using T-PER Tissue protein extraction reagent (Thermo Fisher Scientific, Waltham, Massachusetts), subjected to SDS-PAGE ...
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bioRxiv - Developmental Biology 2020Quote: ... cells were pelleted and 50μg of protein was quantified by ionic detergent compatible Pierce 660nm Protein Assay Reagent (Thermo Fisher) in 8M Urea ...
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bioRxiv - Biochemistry 2021Quote: Protein concentration of cell lysates was determined using the PierceTM BCA protein assay kit according to manufacturer’s instructions (ThermoFisher Scientific). Samples were adjusted in 5xLDS sample buffer containing 50 mM DTT ...
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bioRxiv - Cancer Biology 2020Quote: ... Supernatant of cell lysates was assayed for total protein content using the BCA protein assay (Thermo Fisher Scientific, Rockford, IL), and 70 μg of total protein per sample was separated in a 7.5% polyacrylamide gel (Invitrogen) ...
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bioRxiv - Cell Biology 2022Quote: ... was used to lyse cell pellets and protein concentration determined using the Micro BCA Protein Assay kit (ThermoFisher Scientific, 232350). 10 μg protein ...
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bioRxiv - Physiology 2022Quote: Protein concentration of cell lysates was determined using the PierceTM BCA protein assay kit according to manufacturer’s instructions (ThermoFisher Scientific). Samples were subsequently subjected to SDS-PAGE and blotted on a nitrocellulose membrane ...
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bioRxiv - Bioengineering 2022Quote: ... Extracted protein from the cell-only control samples was quantified using Pierce Rapid Gold BCA Protein Assay Kit (Thermo Fisher). 10 µg protein from each sample was then analyzed by sodium dodecyl sulfate– polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell debris were pelleted by centrifugation (14,000g, 10min, 4°C) and whole cell protein extract concentration was measured using Qubit Protein Assay Kit (Thermo Fisher). Protein extracts were immediately used for EnzyChrom Catalase Assay Kit and EnzyChrom Glutathione Reductase Assay Kit (BioAssay Systems) ...
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bioRxiv - Cell Biology 2021Quote: ... The protein concentration of clarified cell lysate was then measured using the BCA protein assay kit (Thermo Fisher, Cat# 23227). 5 ∼ 20 μg of total protein per lane was separated by SDS-PAGE gel and transferred to PVDF membrane ...
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bioRxiv - Neuroscience 2019Quote: Whole cell protein lysates were isolated from hippocampal tissues by using M-PER mammalian protein extraction reagent (Thermo Scientific, 78501) and by adding Halt Protease Inhibitor (Thermo Scientific ...
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bioRxiv - Biochemistry 2019Quote: ... Cell-surface proteins were purified using Mem-PER™ Plus Membrane Protein Extraction Kit (Thermo Fisher Scientific, Rockford, lL, USA). Isolated proteins were labeled with Cy3 dye and hybridized to a lectin microarray 27–35 ...
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bioRxiv - Molecular Biology 2019Quote: The protein lysate or exosomal protein from of WT H69 and ∆huPGRN-H69 cells were prepared using M-PER mammalian protein extraction reagent (ThermoFisher) or exosome protein isolation kit (ThermoFisher ...
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bioRxiv - Molecular Biology 2021Quote: ... 60 μg of proteins in cell lysates were separated on a NuPAGE™ 4-12% Bis-Tris Protein Gel (Invitrogen) and transferred onto a nitrocellulose membrane (GE ...
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bioRxiv - Microbiology 2021Quote: ... cytoplasmic proteins and the total cell lysate proteins were denatured and boiled with 4× LDS sample buffer (Cat. #NP0007, Invitrogen) and subjected to immunoblotting analysis with equal protein loading of extracts (~20 µg/lane) ...
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bioRxiv - Microbiology 2021Quote: ... total cell protein was extracted and subjected to co-immunopreciptation (Co-IP) with Dynabeads-Protein G (Thermo Fisher Scientific, USA) followed by the protocol described previously (12).
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bioRxiv - Cancer Biology 2022Quote: ... cells were lysed with 15 μl RIPA buffer and protein concentration was quantified using BCA Protein Assay Kit (Thermo Scientific). OCR measurements were normalized to the protein concentration in each well.
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bioRxiv - Microbiology 2023Quote: ... The protein concentration of the cell lysate was measured by BCA assay (Pierce BCA Protein Assay Kit, Thermo Scientific, USA). The cell pellet was resuspended in 40 μl ddH2O and mixed carefully ...
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bioRxiv - Cell Biology 2023Quote: ... The protein concentrations of the both the tissue and cell lysates were assessed by BCA Protein Assay Kit (ThermoFisher #23227) and protein samples were supplemented with 4x laemmli blue (Bio-Rad #1610747 ...
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bioRxiv - Cancer Biology 2023Quote: ... Total protein concentration of the cell lysates was quantified using the Pierce BCA Protein Assay Kit (ThermoFisher, Cat. No. 23227). Cell lysates were mixed with the sample buffer (with beta mercaptanol ...
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bioRxiv - Plant Biology 2023Quote: ... Surface-exposed proteins were biotinylated and purified using the Pierce™ Cell Surface Protein Biotinylation and Isolation Kit (ThermoFisher Scientific), following the manufacturer’s protocol with some modifications ...
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bioRxiv - Developmental Biology 2023Quote: ... The total protein concentration in the cell lysate was quantified by using BCA protein Assay kit (Thermo Scientific™, 23225).
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bioRxiv - Cell Biology 2023Quote: ... the protein concentration of the cell lysates was measured using a Pierce BCA Protein Assay Kit (23225, Thermo Fisher Scientific). Equal amounts of total protein (1 mg ...
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bioRxiv - Developmental Biology 2023Quote: Membrane proteins were extracted from cell pellets using the Mem-PER™ Plus Membrane Protein Extraction Kit (89842, Thermo Fisher) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: The protein concentration of the cell lysates was determined with the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific). For co-immunoprecipitation ...
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bioRxiv - Neuroscience 2020Quote: ... The inner wall and ceiling were coated with Insect-A-Slip (BioQuip Products #2871C) and Surfasil Siliconizing Fluid (Thermo Fisher Scientific #TS-42800), respectively.
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bioRxiv - Microbiology 2020Quote: ... skin homogenate or 100 µl of insect homogenate was extracted in a 96-well plate with the MagMAX™ CORE Nucleic Acid Purification Kit (Applied Biosystems, A32700) using protocol A in a KingFisher™ Flex Magnetic Particle Processor (Applied Biosystems ...
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bioRxiv - Cell Biology 2019Quote: Approximately 5 whole ovary pairs were dissected in room temperature Grace’s insect media (Lonza, Walkersville, MD, USA or Thermo Fisher Scientific, Waltham, MA), transferred to a 1.5ml microcentrifuge tube containing 50µL of Grace’s media ...
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bioRxiv - Microbiology 2020Quote: ... Reverse transcription was performed with TaqMan Fast Virus 1-Step Master Mix (ThermoFisher, Catalog Numbers 4444432). Briefly ...
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bioRxiv - Microbiology 2020Quote: ... Genomic DNA was isolated from purified virus using the GeneJET genomic DNA purification kit (Thermo Fisher), and the fidelity of the changed regions was verified by Sanger sequencing ...
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bioRxiv - Genetics 2021Quote: RNA was reverse transcribed into cDNA using the Moloney Murine Leukemia Virus (M-MLV; Thermo Fisher) according to the manufacturer’s instructions ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... DEPC treated water and Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) kit (Applied Biosystems, 850 Lincoln Centre Drive ...
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bioRxiv - Immunology 2021Quote: Freshly isolated thymocytes or virus-transduced thymocytes were incubated with 4 μM Indo-1-AM (Invitrogen) in TCM at 37°C for 40 min ...
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bioRxiv - Microbiology 2021Quote: ... Five μL RNA was tested with TaqMan™ Fast Virus One-Step Master Mix (Applied Biosystems) using QuantStudio 3 Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Microbiology 2021Quote: ... Five μL RNA was tested with TaqMan™ Fast Virus One-Step Master Mix (Applied Biosystems) using QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Microbiology 2020Quote: ... Clarified virus working stock supernatant was overlaid onto a 25% sucrose-NTE (100nM NaCl (ThermoFisher Scientific), 10mM Tris (Promega ...
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bioRxiv - Microbiology 2021Quote: ... Extracted RNA was amplified using TaqMan™ Fast Virus 1-Step Master Mix (Thermo Fisher Scientific) according to the manufacturers’ protocol ...
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bioRxiv - Neuroscience 2021Quote: ... Reprogramming to pluripotency was carried out using the CytoTune 2.0 sendai virus kit (Thermo-Fisher Scientific) to overexpress four transcription factors (OCT4 ...
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bioRxiv - Microbiology 2020Quote: ... unbound virus was removed by washing three times with PBS (Life Technologies, Carlsbad, California, United States). Next ...
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bioRxiv - Microbiology 2020Quote: ... The virus in the growth medium was recovered by Polyethylene Glycol 8000 (PEG-8000, Fisher Scientific) precipitation ...
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bioRxiv - Biophysics 2020Quote: Virus was attached to a gold-coated probe using dithiobis succinimidyl propionate (DSP) linkers (Thermo Scientific). Dithiobis succinimidyl propionate (DSP ...
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bioRxiv - Cell Biology 2020Quote: ... and sendai-virus iPSCs were cultured on matrigel-coated plates (Geltrex™ LDEV-Free, ThermoFisher Scientific) in Essential 8™ medium (ThermoFisher Scientific ...