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Citations for Thermo Fisher :
1301 - 1350 of 10000+ citations for Dengue Virus Serotype 2 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2020Quote: ... expanded PBMCs were transduced with non-integrating Sendai reprogramming virus (Cytotune 2.0, Thermo Fisher, A16517) according to manufacturer’s instructions added to the Expansion Media ...
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bioRxiv - Neuroscience 2021Quote: ... The viral DNA samples were prepared for quantification by treating the virus with DNaseI (Invitrogen) and Proteinase K ...
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bioRxiv - Microbiology 2020Quote: The nasal swab was initially stored in virus transport medium [Hank’s Balanced Salt Solution (Gibco) containing 2% FCS (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2022Quote: ... qPCRs were set up using TaqMan™ Fast Virus 1-Step Master Mix (Thermo Fisher) and run on an ABI 7500 Fast Real-time PCR System (Applied Biosystems/Thermo Fisher) ...
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bioRxiv - Microbiology 2022Quote: ... the virus inoculum was previously activated with trypsin (200 μg/ml, Gibco Cat. 27250-18) for 1 h at 37 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... The Virus was washed and eluted in Hank’s balanced salt solution (Gibco™, Dublin, Ireland) containing 0.014% Tween-20 (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: Purified virus stocks were diluted in lithium dodecyl sulfate (LDS) sample buffer (Thermo Fisher Scientific) and separated by SDS-PAGE (NuPAGE Novex 10% Bis-Tris gel ...
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bioRxiv - Neuroscience 2022Quote: ... and reverse transcription was performed using Moloney murine leukemia virus reverse transcriptase (Thermo Fisher Scientific). Real-time polymerase chain reaction (PCR ...
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bioRxiv - Synthetic Biology 2023Quote: ... and the precipitated virus pellet was resuspended with 1.1 mL of Opti-MEM (Gibco #31985062) by pipetting and vortexing until fully dissolved ...
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bioRxiv - Microbiology 2023Quote: ... RT-PCR was performed with TaqMan Fast Virus 1-Step Master Mix (Invitrogen, cat. 4444434) in QuantStudio 7 Flex Real-Time PCR System (Thermo Fisher) ...
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bioRxiv - Immunology 2023Quote: ... vRNA was reverse transcribed using the TaqMan Fast Virus 1-Step qRT-PCR kit (Invitrogen) and quantified on a LightCycler 480 instrument (Roche ...
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bioRxiv - Microbiology 2023Quote: ... Virus samples at variable dilutions (1:10 to 1:40,000) were mixed with FluoSphere (Invitrogen) beads (170nm ...
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bioRxiv - Biochemistry 2024Quote: ... Virus generation was performed using the Bac-to-Bac™ expression system (Thermo Fisher Scientific) in Sf9 insect cells ...
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bioRxiv - Microbiology 2024Quote: ... The virus genome was further amplified by PCR (Phusion High-Fidelity DNA Polymerase, ThermoFisher Scientific) with 10 μL cDNA in each 50 μL reaction and MBTuni-12/13 (5′-ACGCGTGATCAGTAGAAACAAGG ...
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bioRxiv - Cell Biology 2020Quote: ... or anti-goat RFC1 antibody (2 μg/sample) coupled to Protein G agarose beads (Thermo Fisher Scientific) for 2 h on an end-over-end rotator at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... proteins were transferred to the PVDF membrane by using iBlot™ 2 Transfer Stacks (Thermo Fisher Scientific) and iBlot™ 2 Gel Transfer Device (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... and the protein concentration was adjusted to 2 mg/ml using the Bradford method (Thermo Scientific, USA). Samples were then reduced using DTT (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2021Quote: ... proteins in the supernatant were reduced with 25 mM tris(2-carboxyethyl)phosphine (TCEP, Thermo Scientific, #20490) for 1 hour at RT ...
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bioRxiv - Synthetic Biology 2019Quote: ... The proteins were transferred from the gel to a nitrocellulose membrane using a iBlot 2 device (ThermoFisher) and the membrane was immunostained using an iBind instrument (ThermoFisher) ...
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bioRxiv - Bioengineering 2021Quote: ... and probed with SARS-CoV/SARS-CoV-2 Spike Protein S2 Monoclonal Antibody (1A9) (ThermoFisher # MA5-35946) and donkey anti-mouse secondary antibodies tagged with an infrared fluorophore (Rockland Immunochemicals) ...
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bioRxiv - Cancer Biology 2022Quote: ... The RNP complex was assembled at 1:2 molar ratio of TrueCut™ Cas9 Protein v2 (Invitrogen) to synthesised gRNA ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were then transferred onto a PVDF membrane using iBlot 2 transfer system (Thermo Fisher Scientific IB24001). Membrane was blocked in 5% Thermo Scientific™ Oxoid™ Skim Milk Powder (Fisher Scientific OXLP0031B ...
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bioRxiv - Microbiology 2019Quote: ... Reacted protein bands were visualized by using Pierce™ ECL 2 Western Blotting Substrate (Thermo scientific, USA) and imaging.
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bioRxiv - Molecular Biology 2021Quote: ... Flag tagged proteins were immunoprecipitated from 2-3mg cellular extracts with Pierce Anti-DYKDDDDK Magnetic Agarose (Invitrogen). Beads were washed 5x with 1ml IP lysis buffer end eluted with 50µl 1xLaemmli buffer ...
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bioRxiv - Developmental Biology 2019Quote: ... TEV protease (a gift from Dirk Görlich) and Histone1-Alexa-488 protein (2 mg/ml, Thermo Fisher) were injected to the desired embryos using Microinjector FemtoJet® (Eppendorf ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with 1% antibiotic-antimycotic and 1% protein-free hybridoma medium II (PFHM 2) (Thermo Fisher Scientific) and the respective growth factors and small molecules (8 µM GSK-3 inhibitor CHIR99021 (R&D Systems) ...
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bioRxiv - Microbiology 2021Quote: ... The purified protein was finally buffered with PBS and quantified using Qubit 2 Fluorometer (Thermo Fisher Scientific), and stored at −80 °C until use.
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bioRxiv - Cell Biology 2022Quote: ... was followed by protein transference to PVDF membranes using an iBlot 2 transfer system (Thermo Fisher Scientific). Membranes were incubated for 45 min in Rockland blocking buffer (Rockland ...
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bioRxiv - Plant Biology 2022Quote: ... Proteins were reduced and alkylated by adding tris(2-carboxyethyl)phosphine (TCEP) (77720, Thermo Scientific, United States) and iodoacetamide (IAA ...
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bioRxiv - Biochemistry 2023Quote: ... and rabbit anti-SARS-CoV-2 spike protein (RBD) polyclonal antibody (Thermo Fisher Scientific; catalog # PA5-114451).
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bioRxiv - Systems Biology 2023Quote: ... Proteins were transferred to PVDF membranes using iBlot 2 dry blotting system (Thermo Fisher Scientific Cat# IB21001). Primary antibodies were used at concentrations recommended by the vendor (see Supplementary Table 3 for antibody information) ...
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bioRxiv - Cell Biology 2023Quote: ... followed by 2 hour incubation at 4℃ with 50 µL of magnetic Dynabeads Protein G (Invitrogen, #10004D). Beads were washed with 1x TBS Tween-20 washing buffer (Thermo Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were transferred onto nitrocellulose membranes using an iBlot™ 2 Dry Blotting System (Thermo Fisher Scientific). Total protein profile on the membranes was stained using Revert™ 700 Total Protein Stain following instruction (LI-COR ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were then transferred to a nitrocellulose membrane using the Invitrogen iBlot 2 transfer device (ThermoFisher: IB21001), blocked for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... #2 or IgG (rabbit; self-made) were coupled per µl magnetic Protein G dynabeads (Life Technologies 10004D) for 1 h at RT ...
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bioRxiv - Neuroscience 2023Quote: ... primary antibodies for mouse anti-MAP2 (microtubule-associated protein 2; Invitrogen, Catalog No. 13-1500, 1:1000) and guinea pig anti-synapsin1/2 (Synaptic Systems ...
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bioRxiv - Systems Biology 2023Quote: ... proteins were transferred onto an iBlot membrane using an iBlot 2 Western Blot Transfer System (Thermo Fisher). Blots were incubated in skim milk solution (50 g/L skim milk powder in TBS-T ...
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bioRxiv - Neuroscience 2020Quote: We dissected brains and ventral nerve cords of 5-7 days old males in Schneider’s insect medium (Thermo Fisher Scientific, Waltham, MA) and fixed in 4% paraformaldehyde (PFA ...
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bioRxiv - Microbiology 2020Quote: ... Bovine blood intake by insects was determined using a SYBR green assay (PowerUp™ SYBR™ Green Master Mix, A25779, Life Technologies) for the detection of bovine mitochondrial cytochrome b as described by Van Der Saag et al ...
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bioRxiv - Immunology 2022Quote: ... Primary B cells were plated at 2-3×106 cells/ml in primary B cell medium (DMEM (Gibco) containing 10% FBS (Sigma) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... media was removed and cells were incubated with standard bath solution (SBS) containing 2 μM Fura-2 acetoxymethyl ester (Fura-2 AM; Thermo Fisher Scientific, Paisley, UK) and 0.01% pluronic acid for 1 h at 37 °C ...
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bioRxiv - Cell Biology 2022Quote: ... cells were prepared using the Subcellular Protein Fractionation Kit for Cultured Cells (78840 from ThermoFisher Scientific), according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: Cells were lysed and proteins were extracted from fresh fibroblast cells with RIPA buffer (Thermo Scientific) supplemented with protease and phosphatase inhibitor mini-tablet (Pierce ...
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bioRxiv - Neuroscience 2019Quote: Isolated taste cells were incubated for 20 minutes in 2 μM Fura 2-AM (Invitrogen, Eugene, OR, USA) and nonionic dispersing agent ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were resuspended in MACS buffer containing 2 µg/mL 4’,6-diamidino-2-phenylindole (DAPI; Life Technologies). A BD FACSAria III Cell Sorter (BD Biosciences ...
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bioRxiv - Neuroscience 2019Quote: ... Cells were passaged 1:2-1:6 every 2-5 days by being rinsed once with DPBS (Gibco) and dissociated using 0.5 mM EDTA (75 μl/cm2 ...
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bioRxiv - Genetics 2020Quote: ... Cells were passaged 1:2-1:6 every 2-4 days by being rinsed once with DPBS (Gibco) and dissociated using 0.5 mM EDTA (75 µl/cm2 ...
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bioRxiv - Microbiology 2022Quote: ... cells were grown in NB medium with Fe0 granules (2 g;1 to 2 mm diameter; Thermo Scientific) as the sole electron donor ...
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bioRxiv - Microbiology 2023Quote: 293T cells (2 × 106) were transfected with 2 μg spike expression vector by lipofection using lipofectamine 2000 (Invitrogen). One day post-transfection ...
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bioRxiv - Microbiology 2023Quote: ... and every 2 hrs a 2 mL treatment culture was stained with Sytox blue dead cell stain (Invitrogen) at a concentration of 1 uM per manufacturer’s protocol and incubated at room temperature for 5 min ...