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Citations for Thermo Fisher :
1351 - 1400 of 10000+ citations for Recombinant Human High Mobility Group Box 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... for 48 h then transfected for 24 h with 1 ug His-G3BP1 3’UTR constructs using Lipofectamine LTX and Plus reagent (Invitrogen). To determine mRNA stability ...
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bioRxiv - Physiology 2021Quote: ... The resulting cell pellet was resuspended in hypotonic buffer and used for Western blotting using HRP-conjugated anti-His antibody (1: 10,000; Novex, Life technologies).
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bioRxiv - Biochemistry 2019Quote: ... blocked with 5% milk in TBST and probed with 6x-His Tag Monoclonal Antibody (HIS.H8) (ThermoFisher MA1-21315, 1:3000). Blots were incubated with primary antibodies overnight at 4 °C with rocking and were then washed (3×5min ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The presence of AhR and ARNT proteins was verified by Western blot using anti-His-tag (mouse monoclonal, MA1-21315, dilution 1:1000, Invitrogen) anti-FLAG-tag (rabbit monoclonal ...
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bioRxiv - Immunology 2021Quote: ... were coated at room temperature for 8 hours with 1 μg/mL 6x-His tag polyclonal antibody (PA1-983B, ThermoFisher), followed by overnight blocking with blocking buffer containing 5% milk/1x PBS/0.01% Tween-20 at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... with a N-terminal FLAG-tag and a C-terminal 6×His-tag preceded by a two-residues linker were subcloned into pFastBac 1 vector (Gibco). Sequence transposition into bacmid DNA using DH10Bac cells (Gibco ...
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bioRxiv - Cell Biology 2022Quote: ... and were maintained in DMEM high glucose (4500 mg/L) supplemented with 10% fetal bovine serum (FBS) (AlphaFBS-HI, Alphabioregen) and 1% Penicillin/Streptomycin antibiotics (Gibco), incubated at 37 °C with 5% CO2 ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 1% FBS (CPS Serum, Cat. No. FBS-500-HI) and 1X penicillin/streptomycin (Gibco, Cat. No. 15140-122), followed by centrifugation in an Eppendorf® centrifuge (model 5810 R ...
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bioRxiv - Molecular Biology 2023Quote: ... and D1-D4 (domains 1-4) with C-terminal 6x-His or StrepII tags were transiently transfected using the Expi293 expression system (ThermoFisher)7 ...
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bioRxiv - Cancer Biology 2019Quote: ... tumor necrosis factor α (TNF-α) and TATA box binding protein (TBP) were determined by real☐time PCR (ABI 7900 Prism, Applied Biosystems, US). Sequences used to amplify a fragment of IL-6 were FP ...
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bioRxiv - Microbiology 2021Quote: ... (eastern white pine) wood chips <2 mm at 750 °C in a modified Fischer Scientific Lindberg/Blue M Moldatherm box furnace (Thermo Fisher Scientific, Waltham, MA, USA) fitted with an Omega CN9600 SERIES Autotune Temperature Controller (Omega Engineering Inc. ...
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bioRxiv - Bioengineering 2019Quote: ... via covalent attachment to COOH groups on the particles via standard EDC chemistry using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (Thermo Fisher Scientific, MA) and N-hydroxysulfosuccinimide sodium salt (Thermo Fisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... in a 1:1:1:1 ratio of human receptor:Gαq-Rluc8:β3:GFP2-γ9 DNA constructs prepared in Opti-MEM (Invitrogen) using a 3:1 ratio of TransIT-2020 (Mirus Bio ...
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bioRxiv - Immunology 2019Quote: ... Samples were prepared using the Ion PGM™ Hi-Q™ View OT2 and Ion PGM™ Hi-Q™ View Sequencing kits (ThermoFisher Scientific) and 4 barcoded libraries were combined per Ion 316™ Chip kit (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2020Quote: ... Flag-tagged BNIP3 was cloned into a pcDNA3.1 vector (Thermo Fisher Scientific) or a pCDH vector (System Biosciences ...
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bioRxiv - Biochemistry 2020Quote: ... Tagged constructs were transfected using Lipofectamine 2000 reagent (Life Technologies, CA, USA) following the manufacturer’s instructions using a 1:3 ratio of DNA:Lipofectamine ...
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bioRxiv - Neuroscience 2020Quote: ... and biotin-tagged DNA was pulled down with streptavidin beads (Invitrogen, 65001) and ligated with Illumina paired-end adapters ...
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bioRxiv - Cell Biology 2022Quote: ... dextran polymers tagged with Alexa Fluor™ 680 (3,000, 10,000 MW, Thermofisher) and dextran tagged with fluorescein (40,000 MW ...
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bioRxiv - Cell Biology 2022Quote: ... GFP-tagged strains were taken from the 4000-GFP yeast library (Thermofisher), while knockout strains were taken from the Yeast Knockout Collection (Invitrogen).
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Enterohepatic Transcription Factor CREB3L3 Protects Atherosclerosis via SREBP Competitive InhibitionbioRxiv - Physiology 2020Quote: ... V5-tagged full-length mouse Creb3l3 cDNA was inserted into pcDNA3.1 (Invitrogen); GFP-tagged full-length mouse Creb3l3 cDNA was inserted into pEGFP (GFP-CREB3L3 ...
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bioRxiv - Microbiology 2019Quote: ... V5 tagged constructs were probed with anti-mouse V5 (Invitrogen #R960-25). Blots were developed using the ECL Plus detection reagent (GE Healthcare ...
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bioRxiv - Molecular Biology 2021Quote: ... These were then tagged using 4 different multiplex TMT isobaric tags (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... corresponding species-specific Alexa-Fluor (488 or 555)-tagged antibodies (Molecular Probes) were incubated for 1h at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... Specific antibody labeling was detected using fluorescence tagged secondary antibodies from Invitrogen. Details of primary antibodies used are as follows ...
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bioRxiv - Immunology 2021Quote: ... was incubated with purified GST-tagged IKKi (catalog # PV4875; Thermo Fisher Scientific) in buffer A containing 20 mM Tris [pH 7.5] ...
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bioRxiv - Bioengineering 2021Quote: ... tagged with Alexa Fluor™ 647 (Microscale Protein Labelling Kit, Thermo Fisher) (HEL-647) ...
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bioRxiv - Molecular Biology 2021Quote: ... Myc-tagged mouse RHBDL4 and GFP were subcloned into pENTR4 vectors (Invitrogen). Recombinant adenovirus plasmids were homologously recombined with pAd/CMV/V5-DEST vectors (Invitrogen) ...
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bioRxiv - Molecular Biology 2020Quote: ... V5-tagged proteins were detected with mouse monoclonal anti-V5 antibody (ThermoFisher) at 0.3 μg/ml ...
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bioRxiv - Molecular Biology 2023Quote: ... GFP-tagged strains were taken from the 4000-GFP yeast library (Thermofisher), RFP-tagged strains were taken from the localization database collection (Huh et al ...
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bioRxiv - Immunology 2023Quote: ... tagged ribosomes were eluted with 1mg/ml HA peptide (Thermo Scientific 26184) for 15 minutes at 30°C ...
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bioRxiv - Pathology 2024Quote: ... Wash with PBS and stain fluorescence-tagged rabbit secondary antibodies (Invitrogen, USA) for visualization ...
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bioRxiv - Cell Biology 2020Quote: ... was used at a 1:1000 dilution overnight at 4°C followed by incubation with fluorescently tagged goat anti mouse-A647 and donkey anti-rabbit-A555 secondary antibodies at 1:100 dilution (Invitrogen, Carlsbad, CA). TUNEL staining to detect apoptotic cells was completed prior to antibody stainig per manufactuors instructions (Promega ...
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bioRxiv - Developmental Biology 2020Quote: ... The next day the antibody was washed five times in PBST 0.1% with gentle agitation at room temperature (30 minutes each) and fluorescently-tagged by horseradish peroxidase-catalysed signal amplification (Thermo Scientific, T20913)
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bioRxiv - Cell Biology 2020Quote: ... AHA-labeled proteins were detected with 1 μM alkyne-tagged Alexa Fluor-488 using Click-iT cell detection reagents (Thermo Fisher Scientific) for 45 min at room temperature as previously described (3).
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bioRxiv - Neuroscience 2021Quote: Bacterial expression constructs coding for His6/SUMO-tagged fusion proteins of rat Shank3 (residues 1-348, SPN-Ank) were generated in pET-SUMO (Thermo Fisher Scientific) as described before (17) ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibodies were washed three times with washing buffer (PBS containing 0.7% Triton X-100) before incubation with secondary antibodies (tagged with Cy2, Cy3, or Cy5; dilution 1:500; Life Technologies Inc., USA) for 1 h at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... This was followed by staining with the corresponding fluorescent tagged secondary antibodies for 1 hour at room temperature: goat anti-rabbit Alexa Fluor 647 (Thermo Fisher Scientific), donkey anti-goat Alexa Fluor 488 (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... sections were rinsed in PBS and incubated in AlexaFluor-647-tagged goat anti-mouse IgG (1:100; Thermo Fisher Scientific, catalog #A21039), AlexaFluor-647-tagged donkey anti-mouse IgG ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies were washed three times with washing buffer (PBS containing 0.7% Triton X-100) before incubation with secondary antibodies (tagged with Cy2, Cy3, or Cy5; dilution 1:500; Life Technologies Inc., USA) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... E14.5 Apcdd1 mutant and wildtype control brains were cryosectioned at 16 microns and immunolabeled with Alexa488-tagged Isolectin (1:100; Thermo Fisher Scientific); Calbindin (1:1,1000 ...
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bioRxiv - Neuroscience 2021Quote: ... sections were rinsed in 0.1 M PBS and incubated in Alexa Fluor 647-tagged donkey anti-rabbit IgG (1:500, ThermoFisher, cat# A-31573) for 2 hr at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... or Alexa Fluor 594-tagged goat anti-guinea pig IgG (1:500, Thermo Fisher, cat# A-21202, A-21206 and A-11076) for 1.0 – 1.5 hours at room temperature ...
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bioRxiv - Immunology 2023Quote: ... followed by staining with 1:500 goat anti-rabbit IgG (H+L) secondary antibody tagged with Alexa Fluor 647 (Invitrogen, A-21244) + 1:50 SiglecF-Alexa Fluor 488 (BD ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were transfected with 1μg/well of the HA-tagged Fpn plasmid (Vector Builder, VB220407-1185gaa, Supplemental Figure 1) using Lipofectamine™ 3000 Transfection Reagent (Invitrogen, L3000001).
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bioRxiv - Biochemistry 2023Quote: ... Cells were then again incubated with p65 primary antibody (1:1000) overnight and successively incubated with secondary antibody tagged with Alexa Fluor IgG 555 (#A32732, Thermo Fisher Scientific) as per abovementioned protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... brain slices were washed 3X in 1X TBS and then incubated for 2 hours at RT with a fluorescent-tagged Alexa Fluor 568 anti-mouse secondary antibody (1:500; Life Technologies A11004). Brain sections were then washed 3X in 1X TBS ...
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bioRxiv - Molecular Biology 2023Quote: ... the brain sections were washed for 5 min x3 in 0.1% Tween 20/PBS and incubated in fluorescence tagged secondary antibodies for 2 hours at RT: Alexa Fluor 594 anti-rabbit (1:500, ThermoFisher Scientific, A11037), Alexa Fluor 488 anti-mouse IgG1 (1:500 ...
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bioRxiv - Genetics 2021Quote: ... H3K4me1 Recombinant Polyclonal Antibody (Cat No. 710795, Invitrogen), normal rabbit and mouse IgG (Santa Cruz Biotechnology)
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bioRxiv - Immunology 2022Quote: ... and recombinant murine IL-18 (Thermo Fisher Scientific) at 1 ng/ml unless otherwise specified ...
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bioRxiv - Biochemistry 2021Quote: ... supplemented with recombinant murine IL-3 (Gibco, PMC0035) at 1 μg/mL (BaF3) ...