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Citations for Thermo Fisher :
1301 - 1350 of 10000+ citations for Recombinant Human High Mobility Group Box 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... and RNaseOUT Recombinant Ribonuclease Inhibitor (ThermoFisher Scientific 10777019) according to the protocol provided by the manufactures ...
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Evolutionary conservation of maternal RNA localization in fishes and amphibians revealed by TOMO-SeqbioRxiv - Developmental Biology 2021Quote: ... 0.5 μl of recombinant ribonuclease inhibitor (RNaseOUT, Invitrogen), 0.5 μl of 0.1 M DTT (Invitrogen) ...
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bioRxiv - Microbiology 2020Quote: ... and 0.2 ng/mL recombinant EGF (GIBCO, UK). All cells were cultured at 37°C and 5% CO2 ...
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bioRxiv - Molecular Biology 2021Quote: ... Recombinant adenoviruses were produced in HEK293A cells (Invitrogen) and purified by CsCl gradient centrifugation ...
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bioRxiv - Immunology 2022Quote: ... Recombinant proteins were produced in Expi293F cells (ThermoFisher) by transfection with purified DNA using the ExpiFectamine 293 Transfection Kit (ThermoFisher) ...
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bioRxiv - Bioengineering 2022Quote: ... and BMP4 recombinant protein (Gibco; 5 ng/mL). The media was half-changed every day during the differentiation process ...
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bioRxiv - Cell Biology 2022Quote: ... RNaseOUT Recombinant Ribonuclease Inhibitor 0.4U/µL (Thermo Scientific), Nuclease free water (Thermo Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... Recombinant proteins were produced in Expi293F cells (ThermoFisher) by transfection of DNA using the ExpiFectamine 293 Transfection Kit (ThermoFisher) ...
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bioRxiv - Microbiology 2022Quote: ... Recombinant proteins were produced in Expi293F cells (ThermoFisher) by transfection of DNA using the ExpiFectamine 293 Transfection Kit (ThermoFisher) ...
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bioRxiv - Immunology 2022Quote: ... 0.5 U/μL RNaseOUT Recombinant Ribonuclease Inhibitor (Invitrogen) was added to single cell RT master mix during the loading step and 1 μL of ADT and HTO additive primers (0.2 μM stock ...
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bioRxiv - Immunology 2022Quote: ... and RNaseOUT™ recombinant ribonuclease inhibitor (Thermo Fisher Scientific Inc. ...
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bioRxiv - Molecular Biology 2023Quote: ... and 1000 U/mL recombinant mouse LIF (Invitrogen).
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bioRxiv - Molecular Biology 2023Quote: ... Recombinant magnetic protein G beads (Invitrogen, MA, USA) were added for 1 hour at 4 °C ...
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bioRxiv - Developmental Biology 2023Quote: ... 0.5 μl of recombinant ribonuclease inhibitor (RNaseOUT, Invitrogen), 2 μl of 5 × Maxima RTA buffer (Thermo Scientific) ...
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bioRxiv - Immunology 2023Quote: ... 0.5 U/µL RNaseOUT Recombinant Ribonuclease Inhibitor (Invitrogen) was added to single cell RT master mix during the loading step and 1 µL of ADT and HTO additive primers (0.2 µM stock ...
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bioRxiv - Immunology 2023Quote: ... RNaseOUT™ Recombinant Ribonuclease Inhibitor (Thermo Scientific 10777019) and combined HaltTM Protease and Phosphatase Inhibitor EDTA-free (Thermo Scientific 78443)) ...
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bioRxiv - Developmental Biology 2024Quote: ... 50ng/mL recombinant murine EGF (Thermo Fisher; PMG8041), 100ng/mL recombinant human Noggin (VWR International ...
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bioRxiv - Cell Biology 2023Quote: ... before RNaseOUT Recombinant Ribonuclease Inhibitor (Thermo Fisher Scientific) was added to all samples at a final concentration of 8 U·μl-1 with an incubation at room temperature for 5 min ...
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bioRxiv - Cell Biology 2024Quote: ... rabbit recombinant ANTI-FLAG M2 antibody (Invitrogen 710662), guinea pig anti-bovine insulin (Linco ...
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bioRxiv - Immunology 2019Quote: ... Samples were prepared using the Ion PGM™ Hi-Q™ View OT2 and Ion PGM™ Hi-Q™ View Sequencing kits (ThermoFisher Scientific) and 4 barcoded libraries were combined per Ion 316™ Chip kit (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2020Quote: ... was used at a 1:1000 dilution overnight at 4°C followed by incubation with fluorescently tagged goat anti mouse-A647 and donkey anti-rabbit-A555 secondary antibodies at 1:100 dilution (Invitrogen, Carlsbad, CA). TUNEL staining to detect apoptotic cells was completed prior to antibody stainig per manufactuors instructions (Promega ...
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bioRxiv - Developmental Biology 2020Quote: ... The next day the antibody was washed five times in PBST 0.1% with gentle agitation at room temperature (30 minutes each) and fluorescently-tagged by horseradish peroxidase-catalysed signal amplification (Thermo Scientific, T20913)
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bioRxiv - Cell Biology 2020Quote: ... AHA-labeled proteins were detected with 1 μM alkyne-tagged Alexa Fluor-488 using Click-iT cell detection reagents (Thermo Fisher Scientific) for 45 min at room temperature as previously described (3).
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bioRxiv - Neuroscience 2021Quote: Bacterial expression constructs coding for His6/SUMO-tagged fusion proteins of rat Shank3 (residues 1-348, SPN-Ank) were generated in pET-SUMO (Thermo Fisher Scientific) as described before (17) ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibodies were washed three times with washing buffer (PBS containing 0.7% Triton X-100) before incubation with secondary antibodies (tagged with Cy2, Cy3, or Cy5; dilution 1:500; Life Technologies Inc., USA) for 1 h at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... This was followed by staining with the corresponding fluorescent tagged secondary antibodies for 1 hour at room temperature: goat anti-rabbit Alexa Fluor 647 (Thermo Fisher Scientific), donkey anti-goat Alexa Fluor 488 (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... sections were rinsed in PBS and incubated in AlexaFluor-647-tagged goat anti-mouse IgG (1:100; Thermo Fisher Scientific, catalog #A21039), AlexaFluor-647-tagged donkey anti-mouse IgG ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies were washed three times with washing buffer (PBS containing 0.7% Triton X-100) before incubation with secondary antibodies (tagged with Cy2, Cy3, or Cy5; dilution 1:500; Life Technologies Inc., USA) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... E14.5 Apcdd1 mutant and wildtype control brains were cryosectioned at 16 microns and immunolabeled with Alexa488-tagged Isolectin (1:100; Thermo Fisher Scientific); Calbindin (1:1,1000 ...
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bioRxiv - Neuroscience 2021Quote: ... sections were rinsed in 0.1 M PBS and incubated in Alexa Fluor 647-tagged donkey anti-rabbit IgG (1:500, ThermoFisher, cat# A-31573) for 2 hr at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... or Alexa Fluor 594-tagged goat anti-guinea pig IgG (1:500, Thermo Fisher, cat# A-21202, A-21206 and A-11076) for 1.0 – 1.5 hours at room temperature ...
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bioRxiv - Immunology 2023Quote: ... followed by staining with 1:500 goat anti-rabbit IgG (H+L) secondary antibody tagged with Alexa Fluor 647 (Invitrogen, A-21244) + 1:50 SiglecF-Alexa Fluor 488 (BD ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were transfected with 1μg/well of the HA-tagged Fpn plasmid (Vector Builder, VB220407-1185gaa, Supplemental Figure 1) using Lipofectamine™ 3000 Transfection Reagent (Invitrogen, L3000001).
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bioRxiv - Biochemistry 2023Quote: ... Cells were then again incubated with p65 primary antibody (1:1000) overnight and successively incubated with secondary antibody tagged with Alexa Fluor IgG 555 (#A32732, Thermo Fisher Scientific) as per abovementioned protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... the brain sections were washed for 5 min x3 in 0.1% Tween 20/PBS and incubated in fluorescence tagged secondary antibodies for 2 hours at RT: Alexa Fluor 594 anti-rabbit (1:500, ThermoFisher Scientific, A11037), Alexa Fluor 488 anti-mouse IgG1 (1:500 ...
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bioRxiv - Molecular Biology 2023Quote: ... brain slices were washed 3X in 1X TBS and then incubated for 2 hours at RT with a fluorescent-tagged Alexa Fluor 568 anti-mouse secondary antibody (1:500; Life Technologies A11004). Brain sections were then washed 3X in 1X TBS ...
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bioRxiv - Microbiology 2023Quote: ... Mice were anesthetized with a mixture of ketamine/xylazine and then infected via retroorbital injection of 100µl containing 1×105 genome equivalents (G.E.) of recombinant NrHV diluted in DPBS (Gibco) (N = 5/strain) ...
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bioRxiv - Biochemistry 2023Quote: ... alanine derivatives were used in a concentration of 10 mM and RNaseOUT recombinant ribonuclease inhibitor (1 µl) (Invitrogen) was added ...
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bioRxiv - Genomics 2021Quote: ... The over-expression construct of human LY6 was created by PCR amplification of cDNA by Phusion® High-Fidelity DNA polymerase (Thermo Fisher Scientific), using the following primers LY-6S-Pac1-S-5’-GGAACGTTAATTAACGCTGAAGTTTGTCTGTGCACTA-3’ ...
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bioRxiv - Cell Biology 2023Quote: ... Human embryonic kidney (HEK) 293T cells were from ATCC (cat. #CRL-3216) and were cultured in high-glucose DMEM (Gibco, cat. #41966-052) supplemented with 10% FBS and 1% Penicillin-Streptomycin.
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bioRxiv - Cell Biology 2023Quote: ... were grown in high-glucose DMEM with supplements (1% penicillin/streptomycin, 15% FBS, 1% NEAA, 1 mM sodium pyruvate [Gibco] ...
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bioRxiv - Cell Biology 2023Quote: ... Shinkai) were grown in high-glucose DMEM with supplements (1% penicillin/streptomycin, 10% FBS, 1% NEAA, 1% L-glutamine [Gibco] ...
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bioRxiv - Pathology 2022Quote: ... human CTRP3 (Invitrogen, PA5-115061, rabbit anti-human), α-SMA-Cy3 (Sigma ...
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bioRxiv - Bioengineering 2019Quote: MDA-MB-231 human breast cancer cells were cultured using 1:1 DMEM:F12 medium (Life Technologies) supplemented with 50 µg/ml gentamicin (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2022Quote: Huh7 human hepatocellular carcinoma cells were grown in DMEM/F-12 1:1 medium (Thermo Fisher) supplemented with 10% fetal bovine serum ...
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bioRxiv - Cell Biology 2021Quote: ... Anti-Hec1 (1:1000, human monoclonal) and Anti-phospho-Paxillin (1:100, rabbit polyclonal, pTyr31, Invitrogen). Secondary antibodies diluted in antibody dilution buffer was added along with the conjugated Phalloidin-TRITC (Santa Cruz Biotechnology ...
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bioRxiv - Cell Biology 2021Quote: ... Embryos were stained with mouse-anti-human-ZO-1 1:500 (Thermo Fisher Scientific, 33-9100) or guinea pig-anti-zebrafish-VE-cadherin 1:500 (Paatero ...
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bioRxiv - Plant Biology 2019Quote: ... benthamiana plants were inverted and all aboveground tissues were submerged into a Magenta™ Ga-7 Plant Culture Box (Fisher Scientific, Catalog No. 50-255-176) filled with the Agrobacterium suspension ...
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bioRxiv - Immunology 2022Quote: ... and against human CXCL4L1 (rabbit anti-CXCL4L1, PA5-21944, Invitrogen; 1:50). Samples were then prepared for microscopy using Duolink® mounting medium with DAPI ...
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bioRxiv - Genomics 2020Quote: ... human Malat1 (positive control, Type 1 Probe, VA1-11317, Thermo Fisher Scientific), and bacterial DapB (negative control ...