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Citations for Thermo Fisher :
1501 - 1550 of 10000+ citations for Recombinant Human High Mobility Group Box 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... with or without recombinant IL-1β (50ng/ml)(Invitrogen; Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... 50 ng/ml recombinant EGF (PMG8041, Thermo Fisher Scientific), 10% conditioned medium from a cell line producing Noggin (kind gift from Hans Clevers) ...
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bioRxiv - Immunology 2023Quote: ... Recombinant mouse (rm)IFN-γ was purchased from ThermoFisher Scientific.
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bioRxiv - Immunology 2024Quote: Recombinant proteins were expressed in Expi293F cells (Life technologies). Full-length spike proteins were cloned into the pCAGGS mammalian expression vector as previously described79,80 ...
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bioRxiv - Plant Biology 2019Quote: ... with induction by 0.5 mM IPTG (Isopropyl β-D-1-Thiogalactopyranoside, 16°C, 12 h) and then purified using Ni-NTA His binding resin (Thermo Scientific) according to the manufacturer’s manual ...
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bioRxiv - Synthetic Biology 2023Quote: ... Membranes were incubated overnight (50 rpm, 4°C) with either mouse anti-His monoclonal (Thermo Fisher; 1:3000 in 5% skimmed milk) or anti-IFNa2 primary antibody (abcam ...
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bioRxiv - Molecular Biology 2019Quote: ... high glucose (4.5 g/L) + GlutaMAX and 1 mM sodium pyruvate (Thermo Fisher Scientific) supplemented with 10% Fetal bovine serum (Tetracycline free ...
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bioRxiv - Immunology 2022Quote: ... 1:5,000 diluted Pierce™ High Sensitivity NeutrAvidin™-HRP (Thermo Fisher cat# 31030) or 1:7,500 diluted T7-tag polyclonal antibody-HRP (Thermo Fisher ...
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bioRxiv - Neuroscience 2022Quote: ... Pierce™ High Sensitivity Streptavidin-HRP (Thermo Fisher scientific; western blot 1:30 000) was used to detect biotin in western blots ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Indexing PCRs (25 μL) were performed using 1 x High Fidelity PCR Buffer (Invitrogen), 2 mM MgSO4 ...
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bioRxiv - Molecular Biology 2022Quote: ... and high dose RNAse I (Thermo Fisher #AM2295; 1:5 diluted in cold DPBS) or low dose RNAse I (1:50 dilution for neurons ...
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bioRxiv - Genomics 2023Quote: ... 1 μL of Novex high density TBE sample buffer (cat. LC6678, Thermo Fisher Scientific) was added and 10μL of mixture was loaded into 6% DNA Retardation Gels (cat ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were lysed in lysis buffer containing 1% Triton X-100 (high purity, ThermoFisher), 150 mM NaCl ...
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bioRxiv - Evolutionary Biology 2023Quote: ... ∼1 kilobase homology arms were amplified using Phusion High-Fidelity DNA Polymerase (Thermo Scientific) from genomic DNA of the injection strain w 1118 ...
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bioRxiv - Immunology 2021Quote: ... plate was incubated with HRP conjugated anti-human IgA (1:1000, Thermo Scientific) for 1hour in TBST with 1% non-fat dry milk ...
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bioRxiv - Immunology 2021Quote: ... Anti-human Perforin eF450 (1:100, clone dG9, Invitrogen, cat # 48-9994-42). Cells were analyzed on a BD LSR Fortessa flow cytometer ...
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Targeting MOG to skin macrophages prevents EAE in macaques through TGFβ-induced peripheral tolerancebioRxiv - Immunology 2019Quote: ... Cells were incubated with AF488 goat anti-human IgG1 (1:500, A10631, Invitrogen) for 30 min at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... goat anti-human Alexa-555 (1:1000, Cat. #A-21433, Thermo Fisher Scientific), donkey anti-mouse Alexa-488 (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... B2-Fab was stained with biotinylated goat anti-human IgG (1:1,000, Invitrogen) followed by streptavidin-PE staining ...
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bioRxiv - Bioengineering 2021Quote: ... anti-CD81 (1:300, mouse monoclonal anti-human CD81 antibody, Invitrogen, MA5-13548); anti-CD9 (1:500 ...
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bioRxiv - Bioengineering 2022Quote: ... and nonspecific competitor (0.1 mg/mL human Cot-1 DNA [Thermo Fisher Scientific]). Hybridization was performed in a humidified chamber at 37C overnight ...
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bioRxiv - Microbiology 2021Quote: ... cells were incubated with human anti-mouse ZO-1 (AlexaFluor-488 conjugate; ThermoFisher)(5 μg/mL ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated with goat anti-human IgG Alexa555 (Invitrogen, A-21433, 1:500) in 0.3% BSA/PBS for 30 min at RT ...
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bioRxiv - Microbiology 2022Quote: ... 1-mL aliquots of control human serum (BP2657100, Fisher Scientific, Hampton, NH, USA) were stored at −20 °C and thawed only once prior to use ...
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bioRxiv - Immunology 2022Quote: ... or anti-human kappa goat antibody-HRP conjugated (Invitrogen, A18853, 1:3000 dilution) was added to detect binding of either Fab or IgG ...
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bioRxiv - Neuroscience 2020Quote: ... and mouse anti-human α-synuclein 1:1000 (Thermo Scientific; Rockford, IL, USA) or goat anti-luciferase 1:250 (Novus Biological ...
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bioRxiv - Immunology 2020Quote: ... goat anti-human IgG/HRP (Thermo Fisher: cat. 62-8420, 1:6’000 dilution), or rabbit anti-human IgA/HRP (Dako ...
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bioRxiv - Cell Biology 2019Quote: ... immortalized human myoblasts C25 were grown in GM: 1 Vol medium 199 (Gibco) + 4 Vol DMEM GlutaMAX (Gibco) ...
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bioRxiv - Cell Biology 2022Quote: We used the 1-Step Human Coupled IVT Kit (Thermo Fisher Scientific #88881) to produce Pkd2 protein in vitro ...
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bioRxiv - Neuroscience 2023Quote: ... Blots were probed with mouse anti-human TAU (HT7; 1:50,000; Invitrogen MN1000), chicken anti-GFP (1:1,000 ...
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bioRxiv - Cell Biology 2023Quote: ... and goat anti-human conjugated to Alexa Fluor 647 (1:400, Invitrogen A21445).
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bioRxiv - Cell Biology 2023Quote: ... and Alexa Fluor 647 goat anti-human (Thermo Fisher Scientific; #A21445; 1:1000). Finally ...
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bioRxiv - Cancer Biology 2023Quote: ... 20 ng ml- 1 Human FGF (fibroblast growth factor) basic (all from Gibco); 50 ng ml-1 recombinant human EGF (epidermal growth factor ...
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bioRxiv - Immunology 2023Quote: ... or HRP-conjugated rabbit anti-human IgG Fc antibodies (Invitrogen, 31423, 1:5,000) diluted in blocking buffer were added to the wells incubated with phages or recombinant proteins ...
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An endothelial SOX18-mevalonate pathway axis enables repurposing of statins for infantile hemangiomabioRxiv - Molecular Biology 2024Quote: ... Cells were labelled with 1:300 dilution of anti-human HMGCR (ThermoFisher, PA537367) or HMGCS1 (ThermoFisher ...
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bioRxiv - Immunology 2024Quote: ... 20:1 ratio of Human T-Activator CD3/CD28 Dynabeads (Thermo Fisher Scientific) for 3 days ...
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bioRxiv - Bioengineering 2019Quote: Microparticles of iron oxide (MPIO; diameter 1.08 μm) with p-toluenesulphonyl reactive surface groups (Invitrogen) were covalently conjugated to purified monoclonal rat anti-mouse monoclonal antibodies for MAdCAM-1 ...
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bioRxiv - Neuroscience 2022Quote: RNA from freshly isolated primary microglia in various groups was extracted with TRIzol reagent (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... were from Proteintech Group Inc (Chicago, IL). pAb against claudin-5 (cat. no. 34-1600) was from Invitrogen and mAb against β-actin (cat ...
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bioRxiv - Physiology 2019Quote: RNA was isolated from WT and DKO livers (n=5 per group) using TRIzol (Invitrogen), reverse-transcribed ...
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bioRxiv - Cancer Biology 2020Quote: Differences in proteomes between treatment groups were analyzed by Proteome Discoverer version 2.4 (Thermo Fisher). Comparisons were made between matching sample types and fractions ...
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bioRxiv - Bioengineering 2020Quote: ... cell lysates from different treatment groups were resolved on SDS-PAGE gels (Thermo Fisher Scientific) and transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Genomics 2019Quote: Total RNA was extracted individually (three animals per group) using Trizol (Invitrogen, Carlsbad, CA, USA) followed by DNase digestion and Qiagen RNeasy column purification (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... Olufunmilayo Olopade’s group in RPMI-1640 supplemented with 10% FBS and antibiotic-antimycotic solution (Gibco). Cells were handled and treated (with TM- and/or DMSO ...
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bioRxiv - Cell Biology 2024Quote: One pellet per group and donor was digested by 0.1mg/mL proteinase K (Thermo Fisher) in Tris hydrochloride (50mM ...
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bioRxiv - Cancer Biology 2023Quote: ... Tissues were further incubated for 2 h at room temperature with HRP-tagged secondary antibody of goat anti-mouse (1:1000) (Life Technologies, Thermo Scientific, USA). The sections were then washed with TBST three times ...
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bioRxiv - Genomics 2022Quote: ... the HA-tagged protein of interest was detected with 1:500 diluted HA Tag Alexa Fluor 647 conjugated mouse antibody (ThermoFisher, Catalog no. 26183-A647). After antibody incubation ...
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bioRxiv - Cell Biology 2022Quote: ... incubated overnight with primary antibodies (1:5000 α-TNY1, 1:10,000 Roche Rat-anti-HA, high affinity 3F10, or 1:50,000 Invitrogen α-Histone H3) in 5% non-fat dry milk ...
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bioRxiv - Physiology 2023Quote: ... the medium was changed to maintenance medium (DMEM high glucose #1-26P02-K, M199 EBS #1-21F01-I; both from BioConcept, 1% horse serum (#16050130; Thermo Fisher Scientific), 2% l-glutamine (#25030149 ...
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bioRxiv - Plant Biology 2019Quote: ... The mixture was analyzed by immunoblotting anti-6×His (Invitrogen, #MA1-135), anti-MBP (Santa Cruz Biotechnology ...