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Citations for Thermo Fisher :
1151 - 1200 of 10000+ citations for Recombinant Human High Mobility Group Box 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... Myc-tagged proteins were immunoprecipitated with Protein G Dynabeads (Invitrogen, 10003D) coated with anti-myc antibody (Cell Signaling ...
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bioRxiv - Cell Biology 2019Quote: ... cells were incubated in Alexa tagged secondary antibodies (Life Technologies, Invitrogen) prepared in SG-PBS for 1hr at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... cells were incubated in Alexa tagged secondary antibodies (Life Technologies, Invitrogen) prepared in SG-PBS for 1hr at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... Tagged proteins were visualized using chemiluminescent reagents (Thermo Fisher Scientific 34579).
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bioRxiv - Cell Biology 2023Quote: ... The RFP-tagged organelle markers were purchased from Invitrogen (Carlsbad, CA): CellLight Golgi-RFP BacMam 2.0 (Cat# C10593) ...
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bioRxiv - Molecular Biology 2023Quote: ... 3mM CaCl2 with 250 units of 6xHIS-tagged SUMO protease (ThermoFisher) for scar-free SUMO tag cleavage ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The tagged cDNA fragments were subcloned into expression vector pcDNA3.1(+) (Invitrogen). Transient transfection of HEK293 cells by plasmid DNA was carried out by Lipofectamine 2000 (Invitrogen) ...
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bioRxiv - Biophysics 2024Quote: ... Nitrocellulose membrane was incubated with His6-tagged Monoclonal Antibody - HRP (Invitrogen) with 1:3000 v/v dilution in PBS-T ...
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bioRxiv - Microbiology 2024Quote: ... The bacteria are tagged with FM-4-64 dye (Thermo Scientific). In the confocal microscope study ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then stained with fluorescently tagged secondary antibodies (Thermo Fisher Scientific Cat# A10037 ...
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bioRxiv - Microbiology 2020Quote: ... An aliquot of 50 ng of purified HIV-1 env DNA was used to clone into the pcDNA 3.1D/V5-His-TOPO vector (Invitrogen) and MAX Efficiency Stlb2 competent cells (Life Technology ...
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bioRxiv - Immunology 2020Quote: ... were coated at room temperature for 3 hours with 1 μg/mL PolyRab anti-His antibody (ThermoFisher, PA1-983B), followed by overnight blocking with blocking buffer containing 1x PBS ...
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bioRxiv - Immunology 2021Quote: ... FACS-sorted cells were suspended at 0.5×106 cells/mL in RPMI 1640 medium supplemented with 10% HI-FCS and 1% PenStrep (10378016; Gibco). CD14+HLA-DRneg/low/CD14+HLA-DRhigh monocytes were cultured in 96 well plates (200μL/well ...
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bioRxiv - Neuroscience 2023Quote: The plasmid for heterologous expression of fshr-1 in HEK cells was obtained by directionally cloning the fshr-1 cDNA into the pcDNA3.1/V5-His-TOPO vector (Invitrogen). The cDNA sequence of the fshr-1a gene isoform was amplified by PCR using cDNA from mix-staged wild-type C ...
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bioRxiv - Microbiology 2024Quote: ... Membranes were then incubated for 1 h at room temperature with the following primary antibodies: mouse anti-His-Tag (dilution 1:1,000, clone HIS.H8, Invitrogen MA121315), mouse anti-Flag (dilution 1:1000 ...
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bioRxiv - Biochemistry 2024Quote: ... Recombinant AtGNL-6XHIS was detected using an anti-HIS (C-term)/AP antibody at a 1:2,000 v/v dilution (Invitrogen) and CDP-start ...
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bioRxiv - Molecular Biology 2021Quote: ... the spleens of each group were pooled and washed in PBS (T&I, Gangwon-do, Korea) with 1% Penicillin/Streptomycin (Gibco, MA, USA). A 70 μm cell strainer (VWR life science ...
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bioRxiv - Bioengineering 2020Quote: ... 30 and 60 min after induction of neutralization of Col 1 and enzymatic gelation (n=2-5 samples/group) of freshly prepared materials with a Multiskan™ GO Microplate Spectrometer (Thermo Scientific). The following solutions were analyzed ...
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bioRxiv - Biophysics 2023Quote: ... we cryogenically stored the skin samples from each group at −80 °C in a 9:1 ratio of DMEM:DMSO with protease inhibitor (ThermoFisher, A32953, Weltham, MA) until assayed ...
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bioRxiv - Microbiology 2024Quote: ... BMDMs were plated a day in advance on a coverslip lined 24-well plate at 8 x105 cells in 1mL per well, in “BMDM media” supplemented with 10% L929 (20% HI FBS, 1% penicillin-streptomycin, 1% non-essential amino acids (ThermoFisher, cat#11-140-050), 1% sodium pyruvate) ...
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bioRxiv - Developmental Biology 2021Quote: ... fibroblast outgrowths were dissociated and split 1:4 using a recombinant trypsin-like enzyme (TrypLE Select, Invitrogen). Additional fibroblast preparations were commercially available ...
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bioRxiv - Biochemistry 2023Quote: ... 1 mg/mL of recombinant protein was mixed with SYPRO Orange at a 5X final concentration (Invitrogen), and reactions were supplemented with 50 mM HEPES ...
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bioRxiv - Bioengineering 2024Quote: ... Sections were subsequently incubated by primary antibody 1:500 (GFP recombinant rabbit monoclonal antibody; G10362, Life Technologies) for 48 hours at 4°C followed by three PBS washes ...
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bioRxiv - Molecular Biology 2021Quote: ... Then recombinant dsDNA fragment (add to > 400 ng/μl final concentration) or recombinant single-strand DNA (ordered from Invitrogen or IDT ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were transfected with V5-tagged WNT11 and 24 h post transfection proteins were crosslinked for 30 min with 1 mM DSS (Thermo Fisher) in 1 ml PBS + 1mM MgCl2 ...
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bioRxiv - Biochemistry 2021Quote: ... For staining FLAG tagged receptors we used anti-FLAG M1 antibody (1:100) labelled with DyLight594 (Thermo Scientific, Cat. no. 46412) dye in presence of 2 mM CaCl2 ...
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bioRxiv - Cell Biology 2022Quote: ... cells were incubated at RT for 1 hr with an anti-mouse secondary antibody tagged with AlexaFluor488 (Thermo Fisher Scientific, A11001) used at 2 μg/mL in blocking buffer ...
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bioRxiv - Neuroscience 2022Quote: ... 2 × 5 min) and incubated with secondary antibody (AlexaFluor 488-tagged goat anti-rabbit Ab, 1:1000, ThermoFisher Scientific, A-11008) and with Alexa Fluor 633-conjugated Streptavidin (1:400 ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were washed twice with filtered PBS and then incubated with AlexaFluor 555-tagged donkey-anti-rabbit (Molecular Probes, 1:1000) and AlexaFluor 488-tagged donkey-anti-mouse (Molecular Probes ...
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bioRxiv - Biochemistry 2023Quote: ... 24 hours later cells were transfected with 2 ug DNA (mCherry tagged PPP1R15A DNA and empty carrier DNA at a ratio of 1:19) using the lipofectamine LTX system (Life Technologies) at a ratio of 3 μl lipofectamine LTX to 1 μl Plus reagent for every nanogram of DNA in 200 μl of Opti-MEM (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2023Quote: Fifty wildtype or myc-tagged Sox3 expressing animal pole cell explants were crosslinked for 15 minutes with 1% methanol-free formaldehyde (Life Technologies) and the reaction was quenched with 125 mM glycine in 0.5% PBST (Triton X-100) ...
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bioRxiv - Bioengineering 2019Quote: ... Samples were rinsed and re-suspended in flow buffer containing 25 μL mL−1 of each of the following monoclonal anti-human antibodies per sample: anti-human CD3-AF700 (56-0037-42, ThermoFisher), - CD40L/CD154-FITC (11-1548-42 ...
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bioRxiv - Genomics 2021Quote: Human embryonic kidney 293 cells (HEK 293) were maintained in high-glucose Dulbecco’s Modified Eagle Medium (DMEM) (Thermo Fisher Scientific, Waltham, Massachusetts) supplied with 10% fetal bovine serum ...
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bioRxiv - Cancer Biology 2020Quote: Tumor samples with sufficient high-quality RNA (n=5) were analyzed on the Affymetrix GeneChip Human Genome U133 Plus (v.2.0) Array (Affymetrix, Santa Clara, USA) at the Microarray Department of the University of Amsterdam ...
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bioRxiv - Cancer Biology 2021Quote: ... anti-human CD14 (11-0149-42, Thermo Fisher Scientific, 1:100), anti-human CD117 known also as cKIT (12-1178-42 ...
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bioRxiv - Bioengineering 2021Quote: ... including Human PD-L1 and PD-1 were purchased from Invitrogen. Human LAG-3 and Na+K+ATPase antibodies were purchased from Cell Signaling Technology and Santa Cruz Biotechnology Inc ...
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bioRxiv - Cell Biology 2021Quote: ... Anti-Human/Mouse Myod1 (5.8A) (1:100, Thermo Fisher Scientific, MA512902), Anti-Mouse Myosin Heavy Chain (1:1000 ...
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bioRxiv - Cell Biology 2019Quote: ... 5 primary and anti-human Alexa 633 (Thermo Fisher; 1:200) secondary antibodies.
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bioRxiv - Microbiology 2021Quote: ... Secondary antibody (100µL 1:500 anti-human IgG HRP, Invitrogen 31410) was added and plates were incubated for 10 minutes at room temperature shaking at 60rpm ...
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bioRxiv - Microbiology 2021Quote: ... The 1/1000 diluted human anti-RBD monoclonal antibody (Thermo Fisher) was added to cells and incubated in a humidified chamber for 1 hr at RT or overnight at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... Alexa-Fluor-488-labelled anti-human (Molecular Probes; 1:200 dilution), and 5μg/ml Hoechst 33342 (Molecular Probes) ...
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bioRxiv - Neuroscience 2019Quote: ... polyclonal rabbit anti-mouse/human claudin-5 (1:300; Life Technologies), polyclonal rabbit anti-mouse/human ZO-1 (1:200 ...
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bioRxiv - Biochemistry 2021Quote: ... monoclonal mouse anti-human Rpt6 (PSMC5) (Invitrogen, 2SU-1B8, 1:10000), monoclonal mouse anti-human Rpt4 (Enzo ...
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bioRxiv - Cancer Biology 2021Quote: ... supplemented with 1 % human melanocyte growth supplement-2 (0165, Gibco™). All cell lines were cultured in a humidified 37 °C hypoxic cell culture incubator (Xvivo System Model X3 ...
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bioRxiv - Immunology 2020Quote: ... with 1% (v/v) Human AB serum (Invitrogen, Carlsbad, CA, USA) and 2 mM EDTA (Sigma-Aldrich ...
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bioRxiv - Biophysics 2022Quote: ... or monoclonal anti-human NPM1 (IgG1, mouse, 1:500) (325200, Invitrogen) in blocking/permeabilization buffer for 120 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 1% Human Keratinocyte Growth Supplement (HKGS, #10761364 Fisher Scientific) and 1% Pen/Strep.
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bioRxiv - Cancer Biology 2023Quote: ... rabbit monoclonal anti-human p53 protein SP5 (1:200) (Invitrogen, USA) and mouse monoclonal anti-p63 antibody (1:500 ...
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bioRxiv - Bioengineering 2023Quote: ... anti-Human/Mouse MyoD (5.8A) (1:200, Thermo Fisher Scientific, MA512902) and anti-myogenin (F5D ...
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bioRxiv - Microbiology 2023Quote: ... containing 1:400 of Human BD Fc Block (ThermoFisher Scientific, BDB564219) per condition ...