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Citations for Thermo Fisher :
1251 - 1300 of 10000+ citations for Recombinant Human High Mobility Group Box 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: Four male mice per group were given IP injections of EdU (Thermofisher Scientific) 24 hr prior to sacrifice ...
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bioRxiv - Cell Biology 2022Quote: ... total cardiac RNA from three hearts per group was isolated using Trizol (Ambion) as per manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: Total RNA was extracted from a group of 5 embryos using Trizol (Invitrogen) as described previously (Chiquet et al. ...
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bioRxiv - Cancer Biology 2023Quote: ... in 5% D-glucose (vehicle control group) (Thermo Fisher Scientific, Waltham, MA, USA), 80 µg (4.0 mg/kg ...
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bioRxiv - Cancer Biology 2023Quote: ... based on the information from the providing groups: Neurobasal medium (Gibco, Darmstadt, Germany) supplemented with 1 x B27 ...
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bioRxiv - Immunology 2022Quote: Recombinant soluble ICAM-1 protein was expressed in a Drosophila Melanogaster cells using pMT/V5-His vector with inducible promotor (Invitrogen), purified by sequential two-step affinity chromatography on anti-ICAM-1 antibody (clone YN1.1 ...
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bioRxiv - Immunology 2021Quote: ... The efficiency of the expression and purification of recombinant proteins were evaluated by 12% SDS-PAGE and Western blotting by using anti-his tag antibodies (1:3,000) (Thermo Scientific) and HRP-conjugated goat anti-mouse IgG (1:10,000 ...
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bioRxiv - Neuroscience 2020Quote: ... for 48 h then transfected for 24 h with 1 ug His-G3BP1 3’UTR constructs using Lipofectamine LTX and Plus reagent (Invitrogen). To determine mRNA stability ...
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bioRxiv - Physiology 2021Quote: ... The resulting cell pellet was resuspended in hypotonic buffer and used for Western blotting using HRP-conjugated anti-His antibody (1: 10,000; Novex, Life technologies).
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bioRxiv - Biochemistry 2019Quote: ... blocked with 5% milk in TBST and probed with 6x-His Tag Monoclonal Antibody (HIS.H8) (ThermoFisher MA1-21315, 1:3000). Blots were incubated with primary antibodies overnight at 4 °C with rocking and were then washed (3×5min ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The presence of AhR and ARNT proteins was verified by Western blot using anti-His-tag (mouse monoclonal, MA1-21315, dilution 1:1000, Invitrogen) anti-FLAG-tag (rabbit monoclonal ...
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bioRxiv - Immunology 2021Quote: ... were coated at room temperature for 8 hours with 1 μg/mL 6x-His tag polyclonal antibody (PA1-983B, ThermoFisher), followed by overnight blocking with blocking buffer containing 5% milk/1x PBS/0.01% Tween-20 at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... with a N-terminal FLAG-tag and a C-terminal 6×His-tag preceded by a two-residues linker were subcloned into pFastBac 1 vector (Gibco). Sequence transposition into bacmid DNA using DH10Bac cells (Gibco ...
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bioRxiv - Cell Biology 2022Quote: ... and were maintained in DMEM high glucose (4500 mg/L) supplemented with 10% fetal bovine serum (FBS) (AlphaFBS-HI, Alphabioregen) and 1% Penicillin/Streptomycin antibiotics (Gibco), incubated at 37 °C with 5% CO2 ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 1% FBS (CPS Serum, Cat. No. FBS-500-HI) and 1X penicillin/streptomycin (Gibco, Cat. No. 15140-122), followed by centrifugation in an Eppendorf® centrifuge (model 5810 R ...
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bioRxiv - Molecular Biology 2023Quote: ... and D1-D4 (domains 1-4) with C-terminal 6x-His or StrepII tags were transiently transfected using the Expi293 expression system (ThermoFisher)7 ...
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bioRxiv - Bioengineering 2019Quote: ... via covalent attachment to COOH groups on the particles via standard EDC chemistry using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (Thermo Fisher Scientific, MA) and N-hydroxysulfosuccinimide sodium salt (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... Flag-tagged BNIP3 was cloned into a pcDNA3.1 vector (Thermo Fisher Scientific) or a pCDH vector (System Biosciences ...
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bioRxiv - Biochemistry 2020Quote: ... Tagged constructs were transfected using Lipofectamine 2000 reagent (Life Technologies, CA, USA) following the manufacturer’s instructions using a 1:3 ratio of DNA:Lipofectamine ...
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bioRxiv - Neuroscience 2020Quote: ... and biotin-tagged DNA was pulled down with streptavidin beads (Invitrogen, 65001) and ligated with Illumina paired-end adapters ...
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bioRxiv - Cell Biology 2022Quote: ... dextran polymers tagged with Alexa Fluor™ 680 (3,000, 10,000 MW, Thermofisher) and dextran tagged with fluorescein (40,000 MW ...
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bioRxiv - Cell Biology 2022Quote: ... GFP-tagged strains were taken from the 4000-GFP yeast library (Thermofisher), while knockout strains were taken from the Yeast Knockout Collection (Invitrogen).
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Enterohepatic Transcription Factor CREB3L3 Protects Atherosclerosis via SREBP Competitive InhibitionbioRxiv - Physiology 2020Quote: ... V5-tagged full-length mouse Creb3l3 cDNA was inserted into pcDNA3.1 (Invitrogen); GFP-tagged full-length mouse Creb3l3 cDNA was inserted into pEGFP (GFP-CREB3L3 ...
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bioRxiv - Microbiology 2019Quote: ... V5 tagged constructs were probed with anti-mouse V5 (Invitrogen #R960-25). Blots were developed using the ECL Plus detection reagent (GE Healthcare ...
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bioRxiv - Molecular Biology 2021Quote: ... These were then tagged using 4 different multiplex TMT isobaric tags (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... corresponding species-specific Alexa-Fluor (488 or 555)-tagged antibodies (Molecular Probes) were incubated for 1h at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... Specific antibody labeling was detected using fluorescence tagged secondary antibodies from Invitrogen. Details of primary antibodies used are as follows ...
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bioRxiv - Immunology 2021Quote: ... was incubated with purified GST-tagged IKKi (catalog # PV4875; Thermo Fisher Scientific) in buffer A containing 20 mM Tris [pH 7.5] ...
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bioRxiv - Bioengineering 2021Quote: ... tagged with Alexa Fluor™ 647 (Microscale Protein Labelling Kit, Thermo Fisher) (HEL-647) ...
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bioRxiv - Molecular Biology 2021Quote: ... Myc-tagged mouse RHBDL4 and GFP were subcloned into pENTR4 vectors (Invitrogen). Recombinant adenovirus plasmids were homologously recombined with pAd/CMV/V5-DEST vectors (Invitrogen) ...
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bioRxiv - Molecular Biology 2020Quote: ... V5-tagged proteins were detected with mouse monoclonal anti-V5 antibody (ThermoFisher) at 0.3 μg/ml ...
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bioRxiv - Molecular Biology 2023Quote: ... GFP-tagged strains were taken from the 4000-GFP yeast library (Thermofisher), RFP-tagged strains were taken from the localization database collection (Huh et al ...
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bioRxiv - Immunology 2023Quote: ... tagged ribosomes were eluted with 1mg/ml HA peptide (Thermo Scientific 26184) for 15 minutes at 30°C ...
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bioRxiv - Cell Biology 2019Quote: ... ELISAs were performed using FBLN5 (Fibulin-5) Human ELISA Kit from Fine Test (EH0772) and Thrombospondin 1 (TSP1) Human ELISA Kit from Invitrogen (BMS2100), following the manufacturer’s protocols ...
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bioRxiv - Genetics 2021Quote: ... H3K4me1 Recombinant Polyclonal Antibody (Cat No. 710795, Invitrogen), normal rabbit and mouse IgG (Santa Cruz Biotechnology)
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bioRxiv - Immunology 2022Quote: ... and recombinant murine IL-18 (Thermo Fisher Scientific) at 1 ng/ml unless otherwise specified ...
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bioRxiv - Biochemistry 2021Quote: ... supplemented with recombinant murine IL-3 (Gibco, PMC0035) at 1 μg/mL (BaF3) ...
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bioRxiv - Microbiology 2021Quote: ... 50 ng/mL recombinant murine EGF (Fisher Scientific), 1X P/S ...
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bioRxiv - Microbiology 2021Quote: ... 50 ng/mL recombinant murine EGF (Fisher Scientific), 1X P/S ...
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bioRxiv - Microbiology 2020Quote: ... 20 units of RNaseOUT recombinant ribonuclease inhibitor (Invitrogen), and 200 units of M-MLV reverse transcriptase in a final reaction volume of 20 μL ...
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bioRxiv - Neuroscience 2020Quote: ... GS or DA recombinant proteins (970-2527aa) (Thermofisher), GST-AAK1 (1-501aa ...
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bioRxiv - Cell Biology 2022Quote: ... and 20 ng/mL recombinant M-CSF (Gibco). BMDM were lifted and seeded into experimental plates for treatment at passage 1.
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Enterohepatic Transcription Factor CREB3L3 Protects Atherosclerosis via SREBP Competitive InhibitionbioRxiv - Physiology 2020Quote: ... Recombinant adenoviruses were produced in 293A cells (Invitrogen) and purified via CsCl gradient centrifugation ...
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bioRxiv - Biochemistry 2021Quote: ... Recombinant active GST-TBK1 was from Thermo Fisher/ Life Technologies (#PV3504) ...
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bioRxiv - Molecular Biology 2021Quote: ... RNaseOUT™ Recombinant Ribonuclease Inhibitor (Thermo Fisher Scientific) and SuperScript® III (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... Recombinant proteins were produced in Expi293F cells (ThermoFisher) by transfection with purified DNA using the ExpiFectamine 293 Transfection Kit (ThermoFisher) ...
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bioRxiv - Molecular Biology 2019Quote: ... 2 U RNaseOUT Recombinant Ribonuclease Inhibitor (Invitrogen, 10777019), 10 U SuperscriptII (Invitrogen ...
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bioRxiv - Cell Biology 2019Quote: ... and RNaseOUT™ Recombinant Ribonuclease Inhibitor (Invitrogen™) as per manufacturer’s recommendations ...
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bioRxiv - Genomics 2021Quote: ... 2 U RNaseOUT Recombinant Ribonuclease Inhibitor (Invitrogen, 10777019), 10 U SuperscriptII (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... Recombinant proteins were coated in PBS buffer (Gibco) at the concentration of 1.2 mg/ml of protein per well ...